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A hemocyanin-derived antimicrobial peptide from the penaeid shrimp adopts an alpha-helical structure that specifically permeabilizes fungal membranes ArchiMer
Petit, Vanessa W.; Rolland, Jean-luc; Blond, Alain; Cazevieille, Chantal; Djediat, Chakib; Peduzzi, Jean; Goulard, Christophe; Bachere, Evelyne; Dupont, Joelle; Destoumieux-garzon, Delphine; Rebuffat, Sylvie.
Background. Hemocyanins are respiratory proteins with multiple functions. In diverse crustaceans hemocyanins can release histidine-rich antimicrobial peptides in response to microbial challenge. In penaeid shrimp, strictly antifungal peptides are released from the C-terminus of hemocyanins. Methods. The three-dimensional structure of the antifungal peptide PvHCt from Litopenaeus vannamei was determined by NMR. Its mechanism of action against the shrimp pathogen Fusarium oxysporum was investigated using immunochemistry, fluorescence and transmission electron microscopy. Results. PvHCt folded into an amphipathic α-helix in membrane-mimicking media and displayed a random conformation in aqueous environment. In contact with F. oxysporum, PvHCt bound...
Tipo: Text Palavras-chave: Antimicrobial peptide; Amphipathic helix; Fungi; Membrane bilayer; Nuclear magnetic resonance (NMR); Fluorescence microscopy.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00301/41202/40362.pdf
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A procedure to evaluate the efficiency of surface sterilization methods in culture-independent fungal endophyte studies BJM
Burgdorf,R.J.; Laing,M.D.; Morris,C.D.; Jamal-Ally,S.F..
Extraneous DNA interferes with PCR studies of endophytic fungi. A procedure was developed with which to evaluate the removal of extraneous DNA. Wheat (Triticum aestivum) leaves were sprayed with Saccharomyces cerevisiae and then subjected to physical and chemical surface treatments. The fungal ITS1 products were amplified from whole tissue DNA extractions. ANOVA was performed on the DNA bands representing S. cerevisiae on the agarose gel. Band profile comparisons using permutational multivariate ANOVA (PERMANOVA) and non-metric multidimensional scaling (NMDS) were performed on DGGE gel data, and band numbers were compared between treatments. Leaf surfaces were viewed under variable pressure scanning electron microscopy (VPSEM). Yeast band analysis of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophyte; Fungi; DNA; Surface sterilization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300030
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A test of community assembly rules using foliar endophytes from a tropical forest canopy ArchiMer
Donald, Julian; Roy, Mélanie; Suescun, Uxue; Iribar, Amaia; Manzi, Sophie; Péllissier, Léonie; Gaucher, Philippe; Chave, Jérôme.
1.Community assembly theory assumes that ecological communities are spatially delimited into patches. Within these patches, coexistence results from environmental filtering, competition, and immigration. Truly delineated communities exist in laboratory studies of microbial cultures in Petri dishes, yet empirical tests conducted in continuous environments often use patches defined by convention as opposed to realised boundaries. 2.Here we perform a test of ecological community assembly rules using foliar endophyte communities from a tropical rainforest, where leaves are considered as patches for both fungal and bacterial communities. We determined the diversity of fungal and bacterial endophytes using environmental DNA sequencing of 365 top‐canopy leaves,...
Tipo: Text Palavras-chave: Bacteria; Distribution; French Guiana; Fungi; Metabarcoding; Plant–pathogen interactions; Rainforest.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00606/71808/70296.pdf
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Abiotic effects on the cover and richness of corticolous lichens on Araucaria angustifolia trunks Acta Botanica
Nunes,Lucas; Burle,Giulia; Gumboski,Emerson Luiz; Dechoum,Michele.
ABSTRACT Lichens play a key role in natural ecosystems, as they can function as primary producers, recycle minerals and fix nitrogen. Despite their environmental importance, little is known about lichen ecology in Brazil, and especially about how abiotic factors may influence their spatial distribution. In this study, we aimed to verify how the cover and richness of corticolous lichens on Araucaria angustifolia trunks vary between two different habitats (Forest and Grassland). The photoquadrat sampling method was applied to A. angustifolia trunks. The Coral Point Count software with Excel extensions (CPCe) was used to analyze photographs for lichen cover and richness. Additionally, a redundancy analysis was conducted to estimate how five abiotic and two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascomycota; Corticolous lichens; Environmental selectivity; Fungi; Lichen ecology; Niche overlap.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000100021
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Achieving high groundnut yields Open Agri
C.L.L..
Palavras-chave: Groundnuts; Electrophoresis; Leaf area; Oilseeds; Area; Genes; Tropical zones; Seasons; Fungi; Insecticides.
Ano: 1996 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4186
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Acremonium kiliense infection in a child with chronic granulomatous disease BJID
Pastorino,Antonio Carlos; Menezes,Ulissis Pádua de; Marques,Heloisa Helena de Souza; Vallada,Marcelo G.; Cappellozi,Vera Lúcia; Carnide,Eugénia Maria Grilo; Jacob,Cristina Miuki Abe.
Infection by unusual microorganisms can be one of the clinical manifestations of primary immunodeficiency (PID). We report on a four-month-old child with pneumonia caused by the fungus Acremonium kiliense as the first clinical manifestation of chronic granulomatous disease. We emphasize the importance of an active search for unusual organisms in immunodeficient patients, and a precise diagnosis and early institution of specific treatment against such microorganisms for the reduction of the morbidity and mortality of these patients.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Infancy; Immunodeficiency; Chronic granulomatous disease; Fungi; Acremonium.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600014
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Addition of Amylase from Aspergillus Awamori to the Diet of Broiler Chickens Rev. Bras. Ciênc. Avic.
Morgado,HS; Cysneiros,CSS; Sousa,CM; Stringhini,JH; Ulhoa,CJ; Silva,AS; Fabino Neto,R; Freitas,PVDX; Oliveira,HP; Batista,LHC.
ABSTRACT Two experiments were performed to evaluate the hematological and blood biochemistry parameters, biometry of digestive organs, enzyme activities, protein content and absolute weight of the pancreas of broilers fed pre-starter and pre-starter diets supplemented or not with amylase from Aspergillus awamori. In total, 120 male Cobb chicks were housed in heated cages in each experiment. A completely randomized experimental design, with two treatments (feed with and without amylase) and six replicates per treatment of 10 birds each was applied. The data were subjected to analysis of variance using the F-test at 5% probability level. The dietary amylase addition did not affect hematological and blood biochemistry parameters and the biometry of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; Chickens; Fungi; Nutrition.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400725
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Aeromycological study at the intensive care unit of the "Dr. Manuel Gea Gonzalez" General Hospital BJID
Ríos-Yuil,José Manuel; Arenas,Roberto; Fernández,Ramón; Calderón-Ezquerro,María; Rodriguez-Badillo,Raymundo.
INTRODUCTION: An aeromycological study verifies the presence and quantifies the concentration of fungal propagules in the air. It is very important in the hospital setting because of the increasing numbers of immunosuppressed and severely ill patients. The objective of this study was to determine the concentration of fungi in the air of the intensive care unit (ICU) of "Dr. Manuel Gea González" General Hospital. METHODS: This is a descriptive, observational cross-sectional study. Air samples were obtained with a single stage Thermo-Andersen Viable Particle Sampler (Thermo Electron Corporation -Massachusetts, U.S.A.) in a Petri dish with potato dextrose agar for 15 minutes at two different times (morning and afternoon) and heights (1 and 1.5 meters). The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Air; Sampling; Colony-forming units.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500005
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Agroclimatology of Asian Grain Legumes Chickpea, Pigeonpea, and Groundnut Open Agri
S.M..
Palavras-chave: Pigeonpeas; Chickpeas; Groundnuts; Genetic soil types; Agroclimatic zones; Sole cropping; Land management; Fungi; Grain legumes.
Ano: 1991 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4089
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"Aids" do marandu tem raízes na umidade do solo. Infoteca-e
VALENTIM, J. F..
Quando gostam de um capim, os pecuaristas costumam lhe conferir falsa "universalidade", plantando-o até mesmo em regiões inadequadas a seu cultivo. Foi o que aconteceu com a Brachiaria brizantha cultivar Marandu, lançada pela Embrapa em 1984 e batizada popularmente de braquiarão. Produtiva, rústica e de fácil manejo, ela tornou-se uma das "coqueluches" da pecuária nas últimas décadas e hoje constitui verdadeira monocultura, ocupando cerca de 60 milhões de hectares no Centro-Oeste e Norte do País. Mas como qualquer outro capim, o Marandu possui suas limitações; aliás, uma especialmente importante: ela não tolera excesso de umidade.
Tipo: Artigo de divulgação na mídia (INFOTECA-E) Palavras-chave: Brachiaria bizantha cv Marandu; Capim Marandu; Morte do braquiarão; Humedad; Suelo.; Pastos forrajeros; Brachiaria bizantha; Enfermedades y desórdenes de las plantas; Gramínea forrageira; Doença de planta; Fungo; Umidade do solo.; Forage grasses; Plant diseases and disorders; Fungi; Soil; Humidity..
Ano: 2006 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/505444
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An analysis of the spontaneous mutation rate measurement in filamentous fungi Genet. Mol. Biol.
Baracho,Marta S.; Baracho,Ivanhoé R..
Mutations related to gene methG1 of Aspergillus nidulans were analyzed, in order to study a mathematical model for the determination of the mutation rate per nucleus per generation, in filamentous fungi. A replica plating technique was used to inoculate, in a single operation, 26 colonies of the strain, into Petri dishes containing culture medium, and the nine central colonies were analyzed for size and number of conidia in each colony. Using this technique, several central colonies were analyzed with regard to the appearance of mutation, and the number and type of reversions were determined for each colony. The frequencies obtained for each reversion were analyzed, in order to verify if their distribution was in accordance with that of Greenwood and Yule....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus nidulans; Mutation rate; Spontaneous mutation; Fungi; Filamentous fungi.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100014
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An annotated bibliography of chickpea diseases Open Agri
Y.L..
Palavras-chave: Crops; Chickpeas; Fungi; Pathogens; Peas; Fungicides; Soil amendments; Diseases; Grain legumes; Biological interaction.
Ano: 1978 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4151
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AN ANNOTATED BIBLIOGRAPHY OF PIGEONPEA Open Agri
B.S..
Palavras-chave: Pigeonpeas; Crops; Legumes; Genes; Peas; Mutants; Grain legumes; Fungi; Cowpeas.
Ano: 1980 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3959
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An approach to the study of the fungal deterioration of a classical art material: Mastic varnish Electron. J. Biotechnol.
Romero-Noguera,Julio; López-Miras,María del Mar; Martín-Sánchez,Inés; Ramos-López,José Miguel; Bolívar-Galiano,Fernando.
Mastic, one of the best natural varnishes, is frequently used as protective and finishing layer or as component of oleo-resinous media in paintings, both in the past and currently. However, this resin is affected by complex deterioration processes which can change its characteristics and thus the visual aspect of works of art. The alteration processes caused by radiation have been widely studied, but there is a lack of information on the biodeterioration of this natural product. In this paper, fungi from collections as well as from oil paintings of the Fine Arts Museum of Granada (Spain) were inoculated onto slides covered with mastic. The samples, after an incubation period of 15 days, were analysed by gas chromatography-mass spectrometry (GC-MS) to...
Tipo: Journal article Palavras-chave: Biodeterioration; Fungi; GC-MS; Mastic.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600001
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Antagonism of Trichoderma species on Cladosporium herbarum and their enzimatic characterization BJM
Barbosa,Maria Angélica G.; Rehn,Kurt Georg; Menezes,Maria; Mariano,Rosa de Lima R..
The verrucose caused by Cladosporium herbarum reduces production and quality of Passion fruit (Passiflora edulis Sims.), a largely consumed tropical fruit. This work aimed to investigate the antagonism of Trichoderma species (T. polysporum, T. koningii, T. viride and T. harzianum) against Cladosporium herbarum, and to study the production of extracellular hydrolytic enzymes by the pathogen and the antagonists. The results showed considerable antagonistic potential for the biocontrol of C. herbarum isolates by all Trichoderma species, except T. koningii. The most distinguished effect was observed for T. polysporum. In relation to the pattern of esterase obtained by electrophoresis in poliacrylamyde gel, the major activity presented by the isolates was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passion fruit; Biological control; Extracellular enzymes; Fungi.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200005
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Antimicrobial effectiveness of maleimides on fungal strains isolated from onychomycosis BABT
Gayoso,Carla Wanderley; Lima,Edeltrudes de Oliveira; Souza,Evandro Leite de; Cechinel Filho,Valdir; Trajano,Vinícius Nogueira; Pereira,Fillipe de Oliveira; Lima,Igara Oliveira.
This study aimed to analyze the effectiveness of maleimides as inhibitors on the growth of fungal strains isolated from onychomycosis by the solid medium diffusion procedure. The results showed a promising antifungical activity of the assayed maleimides with formation of fungal growth inhibition halos oscillating between diameter 10 and 23mm. MIC was 100µg/mL for 3,4-dichloro-N-phenyl-methyl-maleimide and 3,4-dichloro-N-phenyl-propil-maleimide and 200µg/mL for 3,4-dichloro-N-phenyl-maleimide, 3,4-dichloro-N-phenyl-ethyl-maleimide and 3,4-dichloro-N-phenyl-buthyl-maleimide.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Onychomycosis; Fungi; Maleimides; Antifungical activity.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500016
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Antimicrobiales para el control de hongos en una dieta artificial y su impacto en la supervivencia y reproducción de Spodoptera frugiperda (J. E. Smith). Colegio de Postgraduados
Zambrano Gutiérrez, Jorge.
Las dietas artificiales son importantes para establecer crías de insectos bajo condiciones controladas de laboratorio. Sin embargo, en estas condiciones también pueden la contaminación de las dietas con hongos. Para evitar la contaminación de las dietas de insectos se utilizan antimicrobiales que en algunas ocasiones ya están presentes en la dieta o bien se deben agregar a ésta. En el laboratorio de Toxicología de Insecticidas la dieta artificial BioServ Inc., Frenchtown, NJ. se contaminaba con hongos. Así mismo se observó que al controlar la contaminación, ésta se podría utilizar en la cría masiva de Spodoptera frugiperda. El objetivo general del presente trabajo fue estimar la supervivencia, reproducción, duración del ciclo de vida, peso de larvas de...
Palavras-chave: Contaminación; Dietas artificiales; Hongos; Cría masiva; Control; Antimicrobiales; Sobrevivencia; Reproducción; Contamination; Artificials diets; Fungi; Mass rearing; Control; Antimicrobials; Survival; Reproduction; Entomología y Acarología; Maestría.
Ano: 2013 URL: http://hdl.handle.net/10521/2034
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Antracnose de pequizeiro. Infoteca-e
ANJOS, J. de R. N. dos; CHARCHAR, M. J. d'A.; GOMES, A. C..
bitstream/CPAC-2009/24722/1/doc_61.pdf
Tipo: Documentos (INFOTECA-E) Palavras-chave: Planta nativa; Plant diseases; Fungal diseases; Indigenous organisms.; Antracnose; Caryocar Brasiliense; Cerrado; Doença Fúngica; Doença de Planta; Fungo; Patogenicidade; Pequi.; Colletotrichum acutatum; Fungi; Pathogenicity..
Ano: 2002 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/560227
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Aportes a la colección de hongos liquenizados del herbario del Instituto de Botánica Carlos Spegazzini (LPS) Boletín de la Sociedad
Lavornia,Juan M; García,Renato A; Rosato,Vilma G; Kristensen,María J; Chayle,Jorge A; Saparrat,Mario N.
El Instituto de Botánica Carlos Spegazzini (IBCS) (UNLP, La Plata) contiene un herbario de hongos (LPS) de aproximadamente 40000 ejemplares, con 4200 especies tipo. El objetivo de este trabajo fue examinar los ejemplares de hongos liquenizados depositados en el IBCS, actualizar su taxonomía y denominación, revisar las especies con determinación dudosa, identificar aquellos no determinados, e incorporarlos en el Herbario LPS. Se estudiaron 192 ejemplares de 11 provincias de Argentina y de Brasil, Uruguay y Francia y de se determinaron en base a su exo-morfología, cortes histológicos y reacciones histoquímicas. También se realizaron Cromatografías en Capa Delgada (TLC) para estudiar los metabolitos presentes. Se estableció la distribución geográfica en...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Herbario LPS; Líquenes; Argentina.
Ano: 2017 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722017000100001
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Architecture and relevance of several strongly adhered biofilms over a polyester imide (PEI) surface BJM
Soncini-Júnior,Giancarlo; Franchetti,Sandra Mara Martins; Marconato,José Carlos.
A microscopical study was undertaken to analyze the eventual fungi adhesion over a polyester imide surface in enameled copper wires. Scanning electron microscopy allowed to observe in these adhered biofilms, a high amount of pigments, hyphae and its enzymatic arsenal probably acting in the surface of the polymer. Due to the highly aromatic nature of this polyester and the residual phenol derivates still present in the reticulated polymer, an antifungal activity could be expected, but no significant changes were noted in fungal growing and in the adhesion process. Additionally, the wires showed completely failure in all insulation properties. The studies aimed to understand and evaluate the great fungi potential that could be explored in biodeterioration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Biofilms; Hyphae; Pigments; Adhesion; Scanning electron microscopy; Polymeric surface; Polyester-imide; Enameled copper wires.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500036
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