|
|
| FAJARDO,THOR V. M.; NISHIJIMA,MARTA; BUSO,JOSÉ A.; TORRES,ANTÔNIO C.; ÁVILA,ANTÔNIO C.; RESENDE,RENATO O.. |
| Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: OYDV-G; LYSV; GCLV; Detection; Coat protein; PCR; Probes; Epidemiology. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300007 |
| |
