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Registros recuperados: 7
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A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach Anais da ABC (AABC)
Alves-Costa,Fernanda A.; Figueiredo,Márcio A.; Lanes,Carlos F.C.; Almeida,Daniela V.; Marins,Luis F.; Wasko,Adriane P..
The presence of higher level of exogenous growth hormone (GH) in transgenic animals could lead to several physiological alterations. A GH transgenic zebrafish (Danio rerio) line was compared to nontransgenic (NT) samples of the species through a DDRT-PCR approach, with the goal of identifying candidate differentially expressed transcripts in brain tissues that could be involved in GH overexpression. Densitometric analyses of two selected amplification products, p300 and ADCY2, pointed to a significant lower gene expression in the transgenic zebrafish (104.02 ± 57.71; 224.10 ± 91.73) when compared to NT samples (249.75 ± 30.08; 342.95 ± 65.19). The present data indicate that p300 and ADCY2 are involved in a regulation system for GH when high circulating...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential display; GH; Growth hormone; Transgenic; Zebrafish.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200017
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Análise de polimorfismos do GH em bovinos da raça Canchim. Repositório Alice
SILVEIRA, L. G. G.; SUGUISAWA, L.; MARTINS, C. L.; REGITANO, L. C. de A.; ALENCAR, M. M. de; FURLAN, L. R.; OLIVEIRA, H. N..
2003
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Bovinos; Gene candidato; GH; Gado de corte; Polimorfismo.
Ano: 2003 URL: http://www.alice.cnptia.embrapa.br/handle/doc/46681
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Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles BJMBR
Martins,F.S.; Celestino,J.J.H.; Saraiva,M.V.A.; Chaves,R.N.; Rossetto,R.; Silva,C.M.G.; Lima-Verde,I.B.; Lopes,C.A.P.; Campello,C.C.; Figueiredo,J.R..
The objective of this study was to determine the effects of GDF-9, IGF-I, and GH alone or combined on preantral follicle survival, activation and development after 1 and 7 days of in vitro culture. Either fresh (non-cultured) or cultured ovarian tissue was processed for histological and fluorescence analysis. For all media tested, the percent of normal follicles was greater when compared to minimum essential medium supplemented (MEM+) alone, except when ovarian tissue was cultured with GDF-9/IGF-I or GDF-9/GH (P < 0.05). Fluorescence analysis showed that the percent of viable follicles after 7 days of culture was similar for non-cultured tissue and for all treatments tested. The percent of primordial follicles was reduced (P < 0.05) and there was a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GDF-9; IGF-I; GH; Culture; Follicle activation; Goat.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800006
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Les manipulations génétiques : comment améliorer la croissance Inra
Houdebine, L.M..
Les récents développements de la génétique moléculaire offrent désormais la possibilité d’isoler virtuellement n’importe quel gène, de le muter in vitro, de modifier ses éléments régulateurs, de le réintroduire dans des cellules ou des organismes entiers (transgénèse) et donc, de moduler à volonté une fonction physiologique donnée. Ces possibilités ont été et sont encore appliquées à la fonction de croissance. Ainsi, les gènes du GRF (Growth Releasing Factor), de la GH (Growth Hormone) et de l’IGF1 (Insulin-like Growth Factor) ont été introduits dans des embryons de divers animaux. Ces transgènes se sont exprimés et ont conduit à une accélération de la croissance et à une augmentation de la taille des animaux accompagnée d’un assez profond changement de...
Tipo: Journal Article Palavras-chave: IGF; GH; EXPRESSION DU GENE; HORMONE DE CROISSANCE; GHRH.
Ano: 1990 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9300034083032453&uri=/notices/prodinra1/2008/03/
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Metabolic and hormone profiles of Holstein x Gyr cows during pre- and postpartum PAB
Franzoni,Ana Paula Saldanha; Gloria,Joana Ribeiro da; Costa,Anna Luiza Belli de Souza Alves; Martins,Ronaldo Alves; Amaral,Thiago Fernandes; Azevedo,Rafael Alves de; Campos,Ernane Ferreira; Coelho,Sandra Gesteira.
Abstract: The objective of this work was to describe the effect of genetic composition on body condition score (BCS), on total lactation production, and on metabolic and hormone profiles of Holstein x Gyr cows under the same pre- and postpartum management conditions. The genetic groups ½ (n=8), – (n=10), and ≥⅞ (n=10) Holstein x Gyr (HG) were evaluated within the same farm, and all of them showed typical lactation curves. The time required to reach peak production did not differ between groups. The groups also did not differ as to time to reach the lowest BCS after calving or to recover BCS after calving, nor as to their BCS values in the week of first ovulation, number of weeks required for first ovulation, and service period. However, ½ HG cows had...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bos indicus; Bos taurus; GH; IGF-1; Lactation curve; Transition period.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300371
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Qualidade do corpo lúteo e do embrião de ovelhas Santa Inês superovuladas com FSH associado à rbST Arq. Bras. Med. Vet. Zootec.
Rocha do Nascimento,I.M.; Sousa Júnior,A.; Castelo Branco,M.A.; Carvalho,Y.N.T.; Mota,L.H.C.M.; Ferreira,S.B.; Souza,I.O.T; Moraes Júnior,F.J.; Leal,T.M.; Souza,J.A.T..
Avaliou-se o efeito da somatotropina bovina recombinante (rbST) sobre a qualidade do corpo lúteo e do embrião e sobre a concentração de progesterona (P4), em ovelhas da raça Santa Inês doadoras de embriões. Foram utilizadas 13 doadoras, distribuídas aleatoriamente em dois grupos, G1 (n=7) e G2 (n=6). Em ambos os grupos, as doadoras foram sincronizadas com implante vaginal por 14 dias, receberam 100µg de PGF2α (Ciosin®) no sétimo dia e foram superovuladas, a partir do 12º dia, com 250UI de FSH (Folltopin-V®) em seis doses decrescentes. No dia da retirada do implante, as doadoras do G2 receberam 125mg de rbST (Boostin®) e foram cobertas por macho Santa Inês a cada 12 horas até o final do estro. No sexto dia após a primeira cobrição, foram avaliadas, por...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovelha; Embrião; GH.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500015
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Single-nucleotide polymorphism analysis of GH, GHR, and IGF-1 genes in minipigs BJMBR
Tian,Y.G.; Yue,M.; Gu,Y.; Gu,W.W.; Wang,Y.J..
Tibetan (TB) and Bama (BM) miniature pigs are two popular pig breeds that are used as experimental animals in China due to their small body size. Here, we analyzed single-nucleotide polymorphisms (SNPs) in gene fragments that are closely related to growth traits [growth hormone (GH), growth hormone receptor (GHR), and insulin-like growth factor (IGF)-1)] in these pig breeds and a large white (LW) control pig breed. On the basis of the analysis of 100 BMs, 108 TBs, and 50 LWs, the polymorphic distribution levels of GH, GHR, and IGF-1 were significantly different among these three pig breeds. According to correlation analyses between SNPs and five growth traits - body weight (BW), body length (BL), withers height (WH), chest circumference (CC), and abdomen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Minipigs; SNP; GH; GHR; IGF-1.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900753
Registros recuperados: 7
Primeira ... 1 ... Última
 

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