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Burgeot, Thierry; Bocquene, Gilles; Porte, C; Dimeet, Joel; Santella, Rm; Garcia De La Parra, L.m.; Pihol-leszkowicz, A.; Raoux, C.; Galgani, Francois. |
Several bioindicators were used to evaluate the biological and genotoxic effects of marine pollutants near large coastal cities in the northwestern part of the Mediterranean Sea. Three target species of teleosts were selected: red mullet Mullus barbatus and 2 types of comber (Serranus hepatus and S. cabrilla). Induction of ethoxyresorufin-O-deethylase (EROD) activity specific for polycyclic aromatic hydrocarbons (PAH) and polychlorinated biphenyls (PCB) was measured in the livers of the fish, and inhibition of acetylcholinesterase (AChE) by organophosphorus insecticides and carbamates was measured in their muscle tissues. Maximal EROD activities (16.8 +/- 2.7 to 19.4 +/- 4.2 pmol min(-1) mg protein(-1)) recorded in red muller near Barcelona (Spain),... |
Tipo: Text |
Palavras-chave: EROD; GST; AChE; Antioxidant enzymes; Biomonitoring; DNA adducts; PAH; Mullus barbatus. |
Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00183/29410/27779.pdf |
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Berger, Nicholas; Anderson, Kym. |
Virtually all countries tax the consumption of wine (and other alcoholic beverages). However, the rates of taxation, and the tax instruments used, vary enormously between countries. This paper details for all OECD and some other countries the consumer tax rates as of 1996, showing specific or ad valorem excise or wholesale sales taxes, import tariffs, export subsidies and value-added or goods-and-services taxes. It also aggregates them into an ad valorem consumer tax equivalent (CTE) at various wine price levels (since many are specific taxes and so their CTE varies with the price). T he consumer tax equivalent tends to be lower the greater a country's per capita production of wine, especially for premium wine. Australia and New Zealand are shown to have... |
Tipo: Presentation |
Palavras-chave: Consumer wine taxation; GST; Excise taxes; Consumer/Household Economics; H21; H22; H23; F13. |
Ano: 1999 |
URL: http://purl.umn.edu/123770 |
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Tsangaris, C.; Kormas, K.; Strogyloudi, E.; Hatzianestis, I.; Neofitou, C.; Andral, Bruno; Galgani, Francois. |
A suite of biomarkers was measured in caged mussels at areas impacted by different anthropogenic activities along the Greek coastline to assess biological effects of environmental pollution. Mussels were caged at coastal sites in the vicinity of major cities, in areas influenced by major industries, agricultural practices and in islands away from known sources of pollution. Biomarkers indicative of neurotoxicity (acetylcholinesterase, AchE), oxidative stress (catalase, CAT), phase 11 biotransformation of xenobiotics (glutathione S-transferase, GST), metal exposure (metallothioneins, MTs) and protein synthesis (RNA:DNA ratio) were measured to assess effects of various types of pollutants. AchE activity proved to be the most responsive biomarker with... |
Tipo: Text |
Palavras-chave: Biomarkers; AchE; CAT; GST; MTS; RNA:DNA ratio; Mytilus galloprovincialis; Mediterranean. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11286/7890.pdf |
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Garcia,Luciano de O.; Okamoto,Marcelo H.; Riffel,Ana Paula K.; Saccol,Etiane M.; Pavanato,Maria A.; Sampaio,Luís André N.. |
The aim of this study was to determine oxidative stress parameters in the liver and gill of Brazilian flounder juveniles (307.0 ± 16.0 g and 30.0 ± 4.0 cm) submitted to different water temperature (17.1, 23.0 and 28.8ºC) for 72 h and maintained at salinity 25‰. After the acclimation of 7 days, in 23ºC, fish were transferred to 200 L tanks containing seawater (salinity 25‰) at 28.8ºC (heat shock), 17.1ºC (cold shock) or 23.0ºC (control), five replicates (five fish tank-1). The sampled collection occurred in 0 (pre-challenge), 3, 24, 48 and 72 h after temperature shock. Flounder exposed to 17.1ºC and 28.8ºC showed significantly higher TBARS levels and GST activity in the liver post-exposition (PE) in relation to the control (23ºC). CAT activity in liver... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catalase; GST; Lipid peroxidation; TBARS; Thermal shocks. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300607 |
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Maciel,Marcos Euzébio; Oliveira,Fausto Koga; Propst,Gustavo Bonfim; Bicalho,Maria da Graça; Cavalli,Iglenir João; Ribeiro,Enilze Maria de Souza Fonseca. |
Individual variability in xenobiotic metabolism has been associated with susceptibility to developing complex diseases. Genes involved in xenobiotic metabolism have been evaluated in association studies; the difficulty of obtaining accurate gene frequencies in mixed populations makes interpretation of the results difficult. We sought to estimate population parameters for the cytochrome P450 and glutathione S-transferase gene families, thus contributing to studies using these genes as markers. We describe the frequencies of six genes (CYP1A1, CYP2D6, CYP2E1, GSTM1, GSTT1, and GSTP1) and estimate population parameters in 115 Euro-descendants and 196 Afro-descendants from Curitiba, South of Brazil. PCR-based methods were used for genotyping, and statistical... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CYP; GST; Population study. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400010 |
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Sato,N.S.; Suzuki,T.; Ueda,T.; Watanabe,K.; Hirata,R.D.C.; Hirata,M.H.. |
Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and supplementation with arginine tRNA for the AGR codon was needed to obtain GST-rTp15 overexpression. Purified fusion protein yields were 1.9, 1.7 and 5.3 mg/l of cell culture for GST-rTp47, GST-rTp17 and GST-rTp15, respectively. The identities of the antigens obtained were confirmed by automated DNA sequencing using ABI Prism 310 and peptide mapping by Finningan LC/MS. These recombinant antigens were evaluated by immuno-slot blot techniques applied to 137 serum samples from patients with a clinical and laboratory diagnosis of syphilis (61 samples), from healthy... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Treponema pallidum; Cloning; Recombinant antigen; Syphilis; GST. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700002 |
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