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A gasometric method to determine erythrocyte catalase activity 56
Siqueira,A.J.S.; Remião,J.O.; Azevedo,A.M.P.; Azambuja,C.R.J..
We describe a new gasometric method to determine erythrocyte catalase activity by the measurement of the volume of oxygen produced as a result of hydrogen peroxide decomposition in a system where enzyme and substrate are separated in a special reaction test tube connected to a manometer and the reagents are mixed with a motor-driven stirrer. The position of the reagents in the test tube permits the continuous measurement of oxygen evolution from the time of mixing, without the need to stop the reaction by the addition of acid after each incubation time. The enzyme activity is reported as KHb, i.e., mg hydrogen peroxide decomposed per second per gram of hemoglobin (s-1 g Hb-1). The value obtained for catalase activity in 28 samples of hemolyzed human blood...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Erythrocyte; Catalase assay; Gasometry.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900006
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Response activity of alveolar macrophages in pulmonary dysfunction caused by Leptospira infection 80
Marinho,M.; Oliveira-Júnior,I. S.; Perri,S. H.V; Peiró,J. R.; Pavanelli,T. F.; Salomão,R..
Leptopspirosis is a syndrome with different clinical manifestations including the most severe and often fatal forms of pulmonary disease of unknown etiology. Pulmonary injury during the inflammatory process has been associated with the excessive number of alveolar macrophages (AMs) and polymorphonuclear leukocytes stimulated in the lungs and with the production of reactive oxygen and nitrogen intermediates and other inflammatory mediators. The aim of the present work was to evaluate the cellular immune response of AMs or inflammatory cells of hamsters during leptospirosis. The activity of AMs was determined by measuring nitric oxide (NO) and protein production as well as inflammatory cell infiltration in bronchoalveolar lavage (BAL) fluid. Pulmonary...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alveolar macrophages; Bronchoalveolar lavage; Gasometry; Pulmonary dysfunction; Hamsters; Leptospira.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100005
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