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CRZ1 regulator and calcium cooperatively modulate holocellulases gene expression in Trichoderma reesei QM6a Genet. Mol. Biol.
Martins-Santana,Leonardo; Paula,Renato Graciano de; Silva,Adriano Gomes; Lopes,Douglas Christian Borges; Silva,Roberto do Nascimento; Silva-Rocha,Rafael.
Abstract Trichoderma reesei is the main filamentous fungus used in industry to produce cellulases. Here we investigated the role of CRZ1 and Ca2+signaling in the fungus T. reesei QM6a concerning holocellulases production. For this, we first searched for potential CRZ1 binding sites in promoter regions of key genes coding holocellulases, as well as transcriptional regulators and sugar and calcium transporters. Using a nearly constructed T. reeseiAcrz1 strain, we demonstrated that most of the genes expected to be regulated by CRZ1 were affected in the mutant strain induced with sugarcane bagasse (SCB) and cellulose. In particular, our data demonstrate that Ca2+ acts synergistically with CRZ1 to modulate gene expression, but also exerts CRZ1-independent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene regulatory network; Cellulase expression; Transcriptional regulation; Calcium sensing.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400401
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Msxb is a core component of the genetic circuitry specifying the dorsal and ventral neurogenic midlines in the ascidian embryo ArchiMer
Roure, Agnes; Darras, Sebastien.
The tail ascidian larval peripheral nervous system is made up of epidermal sensory neurons distributed more or less regularly in ventral and dorsal midlines. Their formation occurs in two-steps: the ventral and dorsal midlines are induced as neurogenic territories by Fgf9/-16/20 and Admp respectively. The Delta2/Notch interaction,then controls the number of neurons that form. The genetic machinery acting between the inductive processes taking place before gastrulation and neuron specification at tailbud stages are largely unknown. The analysis of seven transcription factors expressed in the forming midlines revealed an unexpected complexity and dynamic of gene expression. Their systematic overexpression confirmed that these genes do not interact following...
Tipo: Text Palavras-chave: Ascidian; Gene regulatory network; Transcription factors; Peripheral nervous system; Neurogenic.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00374/48557/48984.pdf
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