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A biolistic process for in vitro gene transfer into chicken embryos BJMBR
Ribeiro,L.A.; Mariani,P.D.S.C.; Azevedo,J.L.; Rech,E.L.; Schmidt,G.S.; Coutinho,L.L..
Chicken embryos kept in culture medium were bombarded using a high helium gas pressure biolistic device. To optimize the factors that affect transformation efficiency, the lacZ gene under control of the human cytomegalovirus immediate early enhancer/promoter was used as a reporter gene. There was an inverse relationship between survival rate and transformation efficiency. The best conditions obtained for high embryo survival and high transformation efficiency were achieved with 800 psi helium gas pressure, 500 mmHg vacuum, gold particles, an 8 cm DNA-coated microparticle flying distance to the embryo and embryo placement 0.5 cm from the center of the particle dispersion cone. Under these conditions, transformation efficiency was 100%, survival rate 25% and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biolistic process; Chicken embryo; Gene transfer; SS-galactosidase; Green fluorescent protein; GFP.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900003
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A snapshot of gene therapy in Latin America Genet. Mol. Biol.
Linden,Rafael; Matte,Ursula.
Gene therapy attempts the insertion and expression of exogenous genetic material in cells for therapeutic purposes. Conceived in the 1960s, gene therapy reached its first clinical trial at the end of the 1980s and by December 2013 around 600 genuine open clinical trials of gene therapy were registered at NIH Clinical Trials Database. Here, we summarize the current efforts towards the development of gene therapy in Latin America. Our survey shows that the number of scientists involved in the development of gene therapy and DNA vaccines in Latin America is still very low. Higher levels of investment in this technology are necessary to boost the advancement of innovation and intellectual property in this field in a way that would ease both the social and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene therapy; Gene transfer; Gene delivery; Viral vector; South America.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200015
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Agronomic performance, chromosomal stability and resistance to velvetbean caterpillar of transgenic soybean expressing cry1Ac gene. Repositório Alice
HOMRICH, M.S.; PASSAGLIA, L.M.P.; PEREIRA, J.F.; BERTAGNOLLI, P.F.; SALVADORI, J.R.; NICOLAU, M.; KALTCHUK-SANTOS, E.; ALVES, L.B.; BODANESE-ZANETTINI, M.H..
The objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Anticarsia gemmatalis; Glycine max; Plant resistance to insects; Gene transfer; Resistência de plantas a insetos; Transferência de genes.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122096
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Agronomic performance, chromosomal stability and resistance to velvetbean caterpillar of transgenic soybean expressing cry1Ac gene. Repositório Alice
HOMRICH, M. S.; PASSAGLIA, L. M. P.; PEREIRA, J. F.; BERTAGNOLLI, P. F.; SALVADORI, J. R.; NICOLAU, M.; KALTCHUK-SANTOS, E.; ALVES, L. B.; BODANESE-ZANETTINI, M. H..
2008
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Anticarsia gemmatalis; Gene transfer; Glycine max; Plant resistance to insects; Soja.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/823033
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Agronomic performance, chromosomal stability and resistance to velvetbean caterpillar of transgenic soybean expressing cry1Ac gene PAB
Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Salvadori,José Roberto; Nicolau,Marcio; Kaltchuk-Santos,Eliane; Alves,Laura Bianchessi; Bodanese-Zanettini,Maria Helena.
The objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticarsia gemmatalis; Glycine max; Plant resistance to insects; Gene transfer.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700003
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Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety BABT
Tan,Dao Xuan; Tuong,Ho Manh; Thuy,Vu Thi Thu; Son,Le Van; Mau,Chu Hoang.
ABSTRACTThis work studied the amplification, cloning and determination of the GmDREB2 gene from the soybean cultivar DT2008 and five Vietnamese local soybean cultivars (DT26, DT51, DVN5, CB, CBD) and designed the vector carrying the structure containing GmDREB2 gene from cultivar DT2008 (best drought tolerant). The coding region of GmDREB2 gene isolated from six soybean cultivars was 480 nucleotides in length, encoding 159 amino acids. The recombinant structure was designed as 35S- GmDREB2- c-myc and its expression was analysed in transgenic tobacco plants. Recombinant DREB2 protein was expressed in five transgenic tobacco lines with molecular weights close to 20 kDa. During the drought conditions, the proline accumulation of the transgenic tobacco lines...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drought-resistant; GmDREB2 gene; Gene transfer; Soybean; Transcription factors.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500651
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Detection and transfer of antimicrobial resistance gene integron in Salmonella Enteritidis derived from avian material Rev. Bras. Ciênc. Avic.
Okamoto,AS; Andreatti Filho,RL; Rocha,TS; Menconi,A; Marietto-Gonçalves,GA.
The expansion of global poultry production has increased the need to reduce or control the agents responsible for economic losses, including Salmonella spp. These bacteria are also of public health concern due to their potential to cause food poisoning, and, more recently, due to the antimicrobial resistance presented by these bacteria. Molecular biology is an important tool currently used in the diagnosis and research studies of main poultry diseases. The present studied analyzed 100 samples of Salmonella Enteritidis (SE) isolated from avian material aiming at detecting the class 1 integron gene, Integroninvolved in antimicrobial resistance, by means of polymerase chain reaction (PCR), and comparing it with plate inhibition test. Subsequently, SE samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Integron; Gene transfer; Resistance to antimicrobials; Salmonella Enteritidis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000300009
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Exploitation of wild relatives of pigeonpea and chickpea for resistance to Helicoverpa armigera Open Agri
Mallikarjuna, N.,( Nalini Mallikarjuna ).
Palavras-chave: Pigeonpeas; Chickpeas; Hybridization; Genes; Gene pools; Interspecific hybridization; Hybrids; Genomes; Gene transfer; Polyploidy.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3283
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In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA Biol. Res.
RIOS,MARIANA; VENEGAS,ALEJANDRO; CROXATTO,HORACIO B.
Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (<A HREF="#rios97">Ríos et al., 1997</A>). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 µg of pure ß-galactosidase (ß-gal) mRNA, 50 µg of pure DNA from the reporter gene ß-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the ß-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-ß-D-galactopyranoside as a substrate. The administration of ß-gal mRNA and pSVBgal...
Tipo: Journal article Palavras-chave: SS-galactosidase; Gene transfer; Oviduct.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300007
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Laboratory Protocols 2008: Maize Nutrition Quality and Plant Tissue Analysis Laboratory AgEcon
Gallicia, L.; Nurit, E.; Rosales, A.; Palacios-Rojas, N..
Tipo: Report Palavras-chave: Zea mays; Maize; Wheats; Triticum; Transgenic plants; Genetic engineering; Gene transfer; Genetic transformation; DNA hybridization; Phenotypes; Selection; Tissue culture; In vitro culture; Biolistics; Research projects; Methods; Molecular cloning; PCR; Crop Production/Industries; F02; F30.
Ano: 2009 URL: http://purl.umn.edu/56177
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Optimization in Agrobacterium-mediated transformation of Anthurium andraeanum using GFP as a reporter Electron. J. Biotechnol.
Zhao,Qing; Jing,Ji; Wang,Gang; Wang,Jie Hua; Feng,Yuan Yuan; Xing,Han Wen; Guan,Chun Feng.
Although Agrobacterium-mediated transformation protocols for many economically important plant species have been well established, protocol for a number of flowering plants including Anthurium andraeanum remains challenging. In this study, we report success in generating transgenic Anthurium andraeanum cv Arizona using Agrobacterium GV3101 strain harboring a binary vector carrying gfp as a reporter gene. The possibility of facilitating the screening process for transgenic plants expressing functional proteins using gfp marker was explored. In order to realize high transformation efficiency, different explant sources including undifferentiated callus pieces and petioles were compared for their regeneration efficiency and susceptibility to...
Tipo: Journal article Palavras-chave: Cocultivation; Gene transfer; Green fluorescent protein; Regeneration.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500009
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Producing recombinant commercial proteins ArchiMer
Cadoret, Jean-paul; Bardor, M; Lerouge, P; Cabigliera, M; Henriquez, Vitalia; Carlier, Aude.
Extraction of natural substances and chemical synthesis are the main sources of pharmaceutical molecules. When possible, one may transfer the gene of the molecule in living cells creating individual factories producing on demand and in a safe way the requested molecule. Today, bacteria, yeast, mammalian cells and plants constitute the main plate-forms for various commercial products. Microalgae present numerous advantages and could offer a powerful tool for the production of commercial molecules in a near future.
Tipo: Text Palavras-chave: Genetics; Recombinant; Algae; Pharmaceuticals; Gene transfer; Biotechnology; Génétique; Recombinant; Algues; Pharmaceutique; Transfert de gène; Biotechnologie.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4045.pdf
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Retroviral-mediated overexpression of human bone morphogenetic protein 2 affects human mesenchymal stem cells during monolayer proliferation: A cautionary note Electron. J. Biotechnol.
Neumann,Alexander J; Alini,Mauro; Archer,Charles W; Stoddart,Martin J.
Background: Retroviral vectors are commonly used for gene transfer applications and they represent an effective way to provide a sustained delivery of a bioactive factor in basic research and tissue engineering applications. Cells that have been transduced with retroviral vectors ex vivo are usually amplified on tissue culture plastic, for a prolonged period of time, in order to obtain sufficient cell numbers prior to the experiment of interest. However, the effect of the transgene product on the transduced cells, during this period of time, is rarely, if ever, investigated. The current study investigated if transduction with a VSG.G pseudotyped retroviral vector expressing human bone morphogenetic protein 2 (Rv.BMP-2) influences the gene expression...
Tipo: Journal article Palavras-chave: Constitutive expression; Controls; Gene transfer; Monolayer expansion; Retrovirus.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200007
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The challenge of vector development in gene therapy BJMBR
Dani,S.U..
Gene therapy is the treatment of diseases based on the transfer of genetic information. Agents that carry or deliver DNA to target cells are called vectors (Latin vector: carrier, deliverer). Ideally, a vector should accommodate an unlimited amount of inserted DNA, lack the ability of autonomous replication of its own DNA, be easily manufactured, and be available in concentrated form. Secondly, it should have the ability to target specific cell types or to limit its gene expression to specific cell types, and to achieve sustained gene expression in the long term or in a controlled fashion. Finally, it should not be toxic or immunogenic. Such a vector does not exist and none of the DNA delivery systems so far available for in vivo gene transfer is perfect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene therapy; Gene transfer; DNA delivery; Viral vectors; Diposomes; Vector development.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200001
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Tissue culture parameters in sweet orange cultivars. Repositório Alice
MENDES, A.F. da S.; CIDADE, L.C.; MANZOLI, G.N.; OTONI, W.C.; SOARES FILHO, W. dos S.; COSTA, M.G.C..
The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotype-specific. Epicotyl explants from 35-day-old seedlings produced significantly more shoots per explant in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was significantly higher in medium with NAA and IBA than with NAA alone.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Citrus sinensis; Biotechnology; Gene transfer; Morphogenesis; Regeneration; Biotecnologia; Transferência gênica; Morfogênese; Regeneração.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122156
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Tissue culture parameters in sweet orange cultivars PAB
Mendes,Amanda Ferreira da Silva; Cidade,Luciana Cardoso; Manzoli,Gabrielle Novais; Otoni,Wagner Campos; Soares Filho,Walter dos Santos; Costa,Marcio Gilberto Cardoso.
The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotype-specific. Epicotyl explants from 35-day-old seedlings produced significantly more shoots per explant in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was significantly higher in medium with NAA and IBA than with NAA alone.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Biotechnology; Gene transfer; Morphogenesis; Regeneration.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800020
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Transfer of tolerance for abiotic stress from African rice (Oryza glaberrima Steud.) into Asian rice (Oryza sativa L.) and molecular characterization of introgression International Rice Research Institute
Ramanantsoanirina, Alain M. J..
xvi, 144 l. : ill. Thesis (Ph.D.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Abiotic stress; Stress tolerance; Gene transfer; Introgression; Oryza glaberrima.
Ano: 2002 URL: http://hdl.handle.net/123456789/420
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Translocações cromossômicas entre trigo e espécies relacionadas e seu potencial uso no melhoramento genético. Repositório Alice
CASASSOLA, A.; BRAMMER, S. P..
2012
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Triticum aestivum; Estresses bióticos; Rearranjo cromossômico; Transferência de genes; Biotic stress; Chromosomal rearrangement; Gene transfer; Wheat.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/946042
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Unveiling crucivirus diversity by mining metagenomic data. Repositório Alice
DE LA HIGUERA, I.; KASUN, G. W.; TORRANCE, E. L.; PRATT, A. A.; MALUENDA, A.; COLOMBET, J.; BISSEUX, M.; RAVET, V.; DAYARAM, A.; STAINTON, D.; KRABERGER, S.; SAWAR-REZA, P.; GOLDSTIEN, S.; BRISKIE, J. V.; WHITE, R.; TAYLOR, H.; GOMEZ, C.; AINLEY, D. G.; HARDING, J. S.; FONTENELE, R. S.; SCHRECK, J.; RIBEIRO, S. G.; OSWALD, S. A.; ARNOLD, J. M.; ENAULT, F.; VARSANI, A.; STEDMAN, K. M..
bitstream/item/216483/1/mBio-2020-de-la-Higuera-e01410-20.full.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Crucivirus; CRESS-DNA viruses; Recombination; Virus evolution; Environmental virology; Gene transfer.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125352
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ปัจจัยต่างๆ ที่มีผลต่อการถ่ายยีนเข้าสู่ปาล์มน้ำมัน Thai Agricultural
Potjamarn Suraninpong; Supawadee Tawaro; Sompong Te-chato.
Oil palm is a highly producing oil plant. The most cultivation area is in the southern part of Thailand. Expended area of oil palm in the others part needs suitable variety. Thus, the object of this study was to improvement of oil palm via gene transfer technique. Embryogenic callus inducing from young leaves of high yield oil palm were cultured on various kinds and concentrations of antibiotics for screening of suitable concentration using for gene transformation. The result showed that hygromycin, kanamycin and phosphinotricin at 20, 200 and 5 mg/l, respectively, were suitable for transformed selection. Cefotaxime concentration at 200 mg/l was suitable for killing of agrobacterium. The study of inoculation time, co-culture time and co-culture...
Tipo: Collection Palavras-chave: Agrobacterium tumefaciens; Oil palm; Gene transfer; Embryogenic callus; Antibiotic; ปาล์มน้ำมัน; การถ่ายยีน; สารปฏิชีวนะ; เอ็มบริโอเจนิคแคลลัส; ระดับความเข้มข้น; แถบดีเอ็นเอ; ใบอ่อน.
Ano: 2011 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5166
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