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In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC. Electron. J. Biotechnol.
Piña-Escutia,José Luis; Vázquez-García,Luis Miguel; Arzate-Fernández,Amaury Martín.
Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.
Tipo: Journal article Palavras-chave: Genetic integrity; Micropropagation; Oceloxóchitl.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100007
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Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials Electron. J. Biotechnol.
Mezzalama,Monica; Crouch,Jonathan H; Ortiz,Rodomiro.
The use of transgenic crops is steadily increasing around the world, led by soybean (based on total area) and maize (in terms of total number of countries). Transgenic maize is grown in at least 17 countries across four continents: Africa, America, Asia and Europe. The comprehensive global spread of transgenic maize has significant implications for organizations involved in germplasm conservation and genetic enhancement; particularly as some countries require a GMO-free declaration when receiving shipments of maize germplasm. This article describes the protocol used by the International Maize and Wheat Improvement Center (CIMMYT) for monitoring unintentional transgene flow in maize genebank and breeding plots. The protocol is based on polymerase chain...
Tipo: Journal article Palavras-chave: Biosafety; Genetic integrity; Germplasm enhancement; GM-maize.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200006
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