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Registros recuperados: 39
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ADRB2 polymorphisms predict the risk of myocardial infarction and coronary artery disease Genet. Mol. Biol.
Wang,Dong-Wei; Liu,Min; Wang,Ping; Zhan,Xiang; Liu,Yu-Qing; Zhao,Luo-Sha.
Abstract Recently, the rs1042713 G > A and rs1042714 C > G polymorphisms in the beta-2 adrenergic receptor (ADRB2) gene were shown to be related to atherosclerosis diseases. Therefore, we performed a systemic meta-analysis to determine whether the two functional polymorphisms are related to the risk of myocardial infarction (MI) and coronary artery disease (CAD). We identified published studies that are relevant to our topic of interest. Seven case-control studies, with a total of 6,843 subjects, were incorporated into the current meta-analysis. Our analysis showed a higher frequency of rs1042713 G > A variant in patients with MI or CAD compared to healthy controls. A similar result was also obtained with the rs1042714 C > G variant under both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beta-2 adrenergic receptor; Genetic polymorphism; Myocardial infarction; Coronary artery disease; Meta-analysis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400433
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Analysis of apolipoprotein E genetic polymorphism in a large ethnic Hakka population in southern China Genet. Mol. Biol.
Zhong,Zhixiong; Wu,Heming; Wu,Hesen; Zhao,Pingsen.
Abstract There is currently no data about the genetic variations of APOE in Hakka population in China. The aim of this study was to analyze the allelic and genotypic frequencies of APOE gene polymorphisms in a large ethnic Hakka population in southern China. The APOE genes of 6,907 subjects were genotyped by the gene chip platform. The allele and genotype frequencies were analyzed. Results showed that the ∊3 allele had the greatest frequency (0.804) followed by ∊2 (0.102), and ∊4 (0.094), while genotype ∊3/∊3 accounted for 65.43% followed by ∊2/∊3 (15.85%), ∊3/∊4 (14.13%), ∊2/∊4 (3.01%), ∊4/∊4 (0.84%), and ∊2/∊2 (0.74%) in all subjects. The frequencies of the ∊4 allele in Chinese populations were lower than Mongolian and Javanese, while the frequencies of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apolipoprotein E; Genetic polymorphism; Hakka; Southern China; Genotyping.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500742
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Analysis of genetic susceptibility to mercury contamination evaluated through molecular biomarkers in at-risk Amazon Amerindian populations Genet. Mol. Biol.
Klautau-Guimarães,Maria de Nazare; D’Ascenção,Renata; Caldart,Fabiana A.; Grisolia,Cesar K.; Souza,Jurandir R. de; Barbosa,Antonio C.; Cordeiro,Célia M.T.; Ferrari,Iris.
We investigated Individual differences in susceptibility to methylmercury (MeHg) contamination and its relationship with polymorphisms of the detoxifying enzyme glutathione S-transferase (GST). In Brazil, some Amerindian tribes from the Amazon region have an increased level of mercury in their hair. Samples of hair and blood were taken from inhabitants of two villages in the Kayabi and Munduruku Amerindian communities to investigate mercury levels in association with genetic polymorphism of GSTs. Other molecular biological markers were also studied, such as hemoglobin, haptoglobin and glucose 6-phosphate dehydrogenase (G-6-PDH). Higher levels of mercury contamination were found in the Kayabi villagers, who had a null genotype (GSTM1 0/0, also denominated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amerindian; Genetic polymorphism; Glutathione S-transferase; Mercury.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500027
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ANRIL rs1333049 C/G polymorphism and coronary artery disease in a North Indian population - Gender and age specific associations Genet. Mol. Biol.
Kaur,Naindeep; Singh,Jagtar; Reddy,Sreenivas.
Abstract Many studies conducted worldwide substantiate a role of genetic polymorphisms in non-coding regions linked with coronary artery disease (CAD). One such single nucleotide polymorphism (SNP) of a non-coding RNA in the INK4 locus (ANRIL) i.e. rs1333049 C/G in the vicinity of cell cycle regulating genes is documented to have a role in CAD risk. In this study we aimed to determine the association of ANRIL rs1333049 C/G with CAD in a North Indian population. Five hundred disease free controls and 500 CAD patients were genotyped using allele specific ARMS-PCR method. High risk association of rs1333049 was seen in both heterozygous and mutant genotypes (OR=2.883, 95% CI=1.475-5.638 and p=0.002 and OR=6.717, 95% CI=3.444-13.102 and p < 0.001...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic polymorphism; Coronary artery disease; North Indian Population; ARMS-PCR; Epidemiology study.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100114
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Arachis hoehnei, the probable B genome donor of Arachis hypogaea based on crossability, cytogenetical and molecular studies Open Agri
Mallikarjuna, N..
Palavras-chave: Genomes; Hybrids; Polyploidy; Cytogenetics; DNA; Groundnuts; Mosses; Genetic polymorphism; Polymorphism; Genetic improvement.
Ano: 2006 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3177
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Association between serotonin 2C receptor gene (HTR2C) polymorphisms and psychopathological symptoms in children and adolescents BJMBR
Paes,L.A.; Torre,O.H. Della; Henriques,T.B.; de Mello,M.P.; Celeri,E.H.R.V.; Dalgalarrondo,P.; Guerra Júnior,G.; dos Santos Júnior,A..
Serotonin 2C receptors (5HT2C) are involved in serotonin-driven dynamic equilibrium adjustments responsible for homeostatic stability in brain structures that modulate behavior and emotions. Single nucleotide polymorphisms (SNPs) from the serotonin 2C receptor gene (HTR2C) have been associated with several neurological and mental disorders, including abnormalities in cognitive and emotional processes. The aim of this study was to evaluate the association between the rs6318 SNP of the HTR2C gene and behavioral characteristics exhibited by children and adolescents based on the Child Behavior Checklist (CBCL/6-18) inventory. Eighty-five psychiatric outpatients between 8 and 18 years of age underwent genotyping of the rs6318 SNP. The CBCL/6-18 scale was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic polymorphism; Serotonin; CBCL/6-18; Children; Adolescents; HTR2C.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800611
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Bovine kappa-casein gene polymorphism and its association with milk production traits Genet. Mol. Biol.
Rachagani,Satyanarayana; Gupta,Ishwar Dayal.
Point mutations in exon IV of the bovine κ-casein (CSN3) gene determine two allelic variants, A and B. These variants were distinguished by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis in the indigenous Sahiwal and Tharparkar cattle breeds. DNA samples (252 Sahiwal and 56 Tharparkar) were analyzed for allelic variants of the CSN3 gene. Polymorphism was detected by digestion of PCR-amplified products with HindIII, HhaI and HaeIII restriction enzymes, followed by separation on 3% agarose gels, and resolved by ethidium bromide staining. Allele A of the κ-casein gene occurred at a higher frequency than allele B, in both Sahiwal and Tharparkar breeds. The genotypic frequencies of AA, AB, and BB in the Sahiwal and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Indian cattle; Κ-casein; Genetic polymorphism; PCR-RFLP.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500015
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Caractérisation de marqueurs génétiques fonctionnels de la nutrition et/ou de l'adaptation (les amylases) chez l'huître creuse Crassostrea gigas : intérêts pour la sélection ArchiMer
Prudence, Marie.
Two amylase genes, A and B, from the oyster Crassostrea gigas were characterized. Using PCR-RFLP, 6 and 4 alleles, respectively, were described for the amylase genes A and B. The roles of A and B amylase genes were investigated experimentally. They are expressed during larval and adult stages, and A transcripts are more abundant than B. The A transcript increases significantly with temperature, in high trophic conditions. However, A and B transcript levels do not change when food quantity increases although amylase activity augments. The level of B is correlated with dietary starch quantities, whereas the amount of A appears to remain constant ; simultaneously amylase activity decreases and the KM increases. These results suggest that expression of B is...
Tipo: Text Palavras-chave: Enzymatic analyse; Genetic polymorphism; Genes; Nutrition; Growth; Environmental effects; Oysters; Amylases; Amylases; Analyse enzymatique; Polymorphisme génétique; Gènes; Nutrition; Croissance; Effets de l'environnement; Huîtres; Amylases; Amylases.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-1700.pdf
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CCR5D32 mutation in three Brazilian populations of predominantly Sub-Saharan African ancestry Genet. Mol. Biol.
Carvalho,Mônica W.P.; Leboute,Ana P.M.; Oliveira,Silviene F.; Sousa,Sandra M.B.; Klautau-Guimarães,Maria de Nazaré; Simões,Aguinaldo L..
This study reports the frequencies of the CCR5D32 mutation of the beta-chemokine 5 gene and discusses the possible effects of past and recent gene flow in three quilombo remnants (Brazilians communities with anthropological African ancestry whose ancestors were escaped slaves): Rio das Rãs, Mocambo, and São Gonçalo in the northeastern region of Brazil. The CCR5D32 allele frequency of the Mocambo population was significantly higher (5.6%) than that found in the Rio das Rãs (1%) and São Gonçalo (0.9%) populations. These differences may reflect different proportions of parental populations in the founders individuals, a founder-effect and/or different histories of inter-ethnic contact. The frequency of the CCR5D32 allele in the Mocambo sample is similar to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CCR5; Genetic polymorphism; Sub-Saharan Afro-derived Brazilian populations; Ethnic-grouspecific marker.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300002
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Characterization of casein gene complex and genetic diversity analysis in indian goats Inra
Rout, P.K.; Kumar, A.; Mandal, A.; Laloë, D.; Singh, S.K.; Roy, R..
Milk protein polymorphism plays an important role in genetic diversity analysis, phylogenetic studies, establishing geographical diversity, conservation decision, and improving breeding goals. Milk protein polymorphism in Indian goat breeds has not been well studied; therefore, an investigation was carried out to analyze the genetic structure of the casein gene and milk protein diversity at six milk protein loci in nine Indian goat breeds/genetic groups from varied agro-climatic zones. Milk protein genotyping was carried out in 1098 individual milk samples by SDS-PAGE at aS1-CN (CSN1S1), b-CN (CSN2), aS2-CN (CSN1S2), j-CN (CSN3), b-LG, and a-LA loci. Indian goats exhibited aS1-casein A allele in higher frequency in the majority of breeds except Ganjam and...
Tipo: Journal Article Palavras-chave:  Casein haplotype; Genetic polymorphism; Genetic structure; Goat.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201140d0a011&uri=/notices/prodinra1/2011/06/
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Combined association of Presenilin-1 and Apolipoprotein E polymorphisms with maternal meiosis II error in Down syndrome births Genet. Mol. Biol.
Bhaumik,Pranami; Ghosh,Priyanka; Ghosh,Sujay; Feingold,Eleanor; Ozbek,Umut; Sarkar,Biswanath; Dey,Subrata Kumar.
Abstract Alzheimer's disease and Down syndrome often exhibit close association and predictively share common genetic risk-factors. Presenilin-1 (PSEN-1) and Apolipoprotein E (APOE) genes are associated with early and late onset of Alzheimer's disease, respectively. Presenilin −1 is involved in faithful chromosomal segregation. A higher frequency of the APOE ε4 allele has been reported among young mothers giving birth to Down syndrome children. In this study, 170 Down syndrome patients, grouped according to maternal meiotic stage of nondisjunction and maternal age at conception, and their parents were genotyped for PSEN-1 intron-8 and APOE polymorphisms. The control group consisted of 186 mothers of karyotypically normal children. The frequencies of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome; Genetic polymorphism; Karyotype; Meiosis; Microsatellite markers.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400577
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Distribution of N-acetyltransferase Type 1 (NAT1) genotypes and alleles in a Turkish population Genet. Mol. Biol.
Arslan,Serdal; Degerli,Naci; Bardakci,Fevzi.
NAT1 is an intronless gene on chromosome 8p21.3 encoding a 290-amino-acid-long protein showing acetyltransferase activity. Some 26 alleles of NAT1 gene have been identified in human populations. In the present study we determined the distributions of NAT1 genotypes and alleles in a sample of 201 individuals from the Turkish population in Central Anatolia. The most frequent genotypes were NAT1*4/NAT1*4 (51.74%), NAT1*10/NAT1*4 (22.39%), NAT1*11/NAT1*4 (7.46), NAT1*10/NAT1*10 (3.98%). Frequencies of NAT1*3, *4 (wild-type), *10 and *11 alleles were 3.73%, 69.6%, 17.66% and 7.2%, respectively. The frequency of NAT1*11 was the highest amongst the populations studied so far, the other allele frequencies being close to those described in Caucasian populations.
Tipo: Info:eu-repo/semantics/article Palavras-chave: NAT1 gene; Genetic polymorphism; Molecular epidemiology; Turkish population.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200005
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Distribution of the CCR5delta32 allele (gene variant CCR5) in Rondônia, Western Amazonian region, Brazil Genet. Mol. Biol.
Farias,Josileide Duarte de; Santos,Marlene Guimarães; França,Andonai Krauze de; Delani,Daniel; Tada,Mauro Shugiro; Casseb,Almeida Andrade; Simões,Aguinaldo Luiz; Engracia,Vera.
Since around 1723, on the occasion of its initial colonization by Europeans, Rondonia has received successive waves of immigrants. This has been further swelled by individuals from northeastern Brazil, who began entering at the beginning of the twentieth century. The ethnic composition varies across the state according to the various sites of settlement of each wave of immigrants. We analyzed the frequency of the CCR5L32 allele of the CCR5 chemokine receptor, which is considered a Caucasian marker, in five sample sets from the population. Four were collected in Porto Velho, the state capital and the site of several waves of migration. Of these, two, from the Hospital de Base were comprised of HB Mothers and HB Newborns presenting allele frequencies of 3.5%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CCR5; CCR5Δ32; Rondônia; Genetic polymorphism; Allele frequency.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100003
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Genetic Analysis of Izoenzymes Polymorphisms in Silkworm (Bombyx mori L.) Strains - doi: 10.4025/actascibiolsci.v35i2.13102 Biological Sciences
Ronqui, Ludimilla; Fundação Universidade Federal de Rondônia; Fernandez, Maria Aparecida; Universidade Estadual de Maringá; Takasusuki, Maria Claudia Colla Ruvolo; Universidade Estadual de Maringá.
This work carried out to evaluates the polymorhism in the silkworm of different lineages using the isoenzymes electrophoresis to detect biochemical markers and to investigate the genetics of populations for those lineages. They were used as samples individual extracts of silk glands of second day old larvas of the fifth instar, originating from seven Japanese lineages and eight pure Chinese lineages maintained by the Cocamar-Cooperativa Agroindustrial de Maringá. The isozymes acid phosphatase (ACP), alkaline phosphatase (AKP) and carbonic anhydrase (CA) they were submitted to the electrophoresis in starch gels 14%. The esterases (EST) were analyzed in polyacrylamide gels to 10% and stained with α and b-naphtyl acetate. The total of 21 loci was detected,...
Palavras-chave: 2.02.04.00-0 silkworm; Genetic polymorphism; Isoenzymes Genética animal.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/13102
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Genetic differentiation in natural populations of Lutzomyia longipalpis (Lutz &amp; Neiva) (Diptera: Psychodidae) with different phenotypic spot patterns on tergites in males Neotropical Entomology
Silva,MH; Nascimento,MDSB; Leonardo,FS; Rebêlo,JMM; Pereira,SRF.
Entomological surveys in the state of Maranhão have recorded morphologically distinct populations of Lutzomyia longipalpis (Lutz &amp; Neiva). Some populations have one pair of spots (1S) on the fourth tergite, while others have two pairs (2S) on the third and fourth tergites of males. In the present study we investigated the degree of genetic polymorphism among four populations in the municipalities of Caxias, Codó and Raposa, in the state of Maranhão, Brazil, by using RAPD (Random Amplified Polymorphic DNA) markers. A total of 35 loci were identified, of which 30 were polymorphic. The highest polymorphism was observed with primer OPA 4, which produced 11 different profiles. Genetic diversity was assessed using grouping methods that produced a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abdominal spot; Genetic polymorphism; Leishmaniasis; RAPD-PCR; Sand fly.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000400015
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Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS BABT
Bittencourt-Oliveira,Maria do Carmo; Cunha,Maristela Casé Costa; Moura,Ariadne do Nascimento.
The escalating occurrence of cyanobacterial toxic blooms demands a better understanding of genetic variability as an auxiliary expedient in species identification, collaborating with the monitoring of water destined to public supply. This study aimed at the unraveling of genetic polymorphism in the toxic and nontoxic strains of Microcystis (Cyanobacteria) species, isolated from diverse Brazilian localities through the RFLP-PCR technique applied to the c-phycocyanin encoding operon and its intergenic spacer (cpcBA-IGS). Eighteen strains belonging to M. aeruginosa, M. panniformis, M. protocystis and M. wesenbergii, plus two other unidentified strains, were analyzed by means of the morphological and molecular data. The molecular data constituted three groups...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyanobacteria; CpcBA-IGS; Genetic polymorphism; Microcystin; Microcystis; RFLP.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400014
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Genetic polymorphism in eight Chilean strains of the carotenogenic microalga Dunaliella salina Teodoresco (Chlorophyta) Biol. Res.
GOMEZ,PATRICIA I; GONZALEZ,MARIELA A.
Eight Chilean strains of Dunaliella salina obtained within a restricted geographic range, but exhibiting a high variability in their morphology, rate of growth and carotenogenic capacity, were analyzed by Random Amplified Polymorphic DNA (RAPD-PCR). Twenty of the 50 random primers (D, P, OPA and OPD series) that were tested amplified reproducible bands and were useful for comparative analysis of the strains. Of 107 polymorphic genetic markers, 49 were strain-specific. A great genetic variability was found among the strains in spite of their geographic proximity. In addition, phenetic analysis of the data showed close agreement between the morpho-physiological attributes and the genetic diversity of the strains
Tipo: Journal article Palavras-chave: SS-carotene; Dunaliella salina; Genetic polymorphism; RAPD.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100012
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Genetic polymorphism of Streptococcus mutans in Brazilian family members BJM
Spolidorio,Denise Madalena Palomari; Höfling,José Francisco; Pizzolitto,Antônio Carlos; Rosa,Edvaldo Antonio; Negrini,Thaís de Cássia; Spolidorio,Luís Carlos.
The aim of this study was to determine whether random amplified polymorphic DNA (AP-PCR) analysis is able to differentiate genetically different clones of mutans streptococci, in 22 Brazilian family members. Stimulated saliva samples were collected from fathers, mothers and infants. For 5-18 months babies with erupting primary dentition, plaque samples were collected using sterile tooth pick tips. From these samples, mutans streptococci were isolated on SB-20 agar plates. After growth, representative colonies were identified by biochemical methods on the basis of carbohydrate fermentation. Streptococcus mutans isolates were obtained from all family members and AP-PCR typed separately with a random primer (OPA-13). Bacterial cell lysates were used as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus mutans; AP-PCR; Genetic polymorphism.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300006
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Genetic relationship among Camponotus rufipes Fabricius (Hymenoptera: Formicidae) nests by RAPD molecular markers - doi: 10.4025/actascibiolsci.v35i1.10913 Biological Sciences
Matta, Sara Lívia da Silva Fernandes da; Universidade de Mogi das Cruzes; Morini, Maria Santina de Castro; Universidade de Mogi das Cruzes; Hilsdorf, Alexandre Wagner Silva; Universidade de Mogi das Cruzes.
Random amplified polymorphic DNA (RAPD) markers were used to investigate the genetic relationship among nests of the carpenter ant, Camponotus rufipes, located in the same area. Five random oligodecamers were used to amplify DNA from 108 ant workers collected from six nests. A total of 47 RAPD markers were identified, which revealed low levels of genetic differentiation among nests (Fst = 0.00218) and a low average Shannon index (0.3727) among workers within nests. These results together suggest that the C. rufipes nest may be formed by a single, once-mated queen and that nests produced by queens that are genetically related tend to keep their nests in close proximity to one other.
Palavras-chave: 2.02.02.00-8 carpenter ants; Monogyne; Genetic polymorphism; Genetic distance genética.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/10913
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Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants PAB
Gomes,Eliane Aparecida; Jardim,Sílvia Neto; Guimarães,Claudia Teixeira; Souza,Isabel Regina Prazeres de; Oliveira,Elizabeth de.
The objective of this work was to characterize the genetic variability of phytoplasma and Spiroplasma kunkelii isolated from maize plants showing symptoms of stunt collected from different Brazilian geographic regions. A DNA fragment of 500 base pairs (bp) was amplified from the spiralin gene in S. kunkelii and one fragment of 1,200 bp was generated from 16S rDNA gene in phytoplasma. The partial sequences of the spiralin gene showed similarity of 98% among the isolates of S. kunkelii analyzed. These sequences were compared with the sequence of the spiralin gene from other Spiroplasma species deposited in the GenBank, resulting in a similarity varying from 76.9% to 88.1%. The 16S rDNA sequence from the phytoplasma were completely similar within the...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Zea mays; Microorganisms; Spiralin; Mollicutes; Genetic polymorphism; Gene sequence.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000100009
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