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SOUSA, A. C. B.; JUNGMANN, L.; CAMPOS, T. de; SFORÇA, D. A.; BOAVENTURA, L. R.; SILVA, G. M. B.; ZUCCHI, M. I.; JANK, L.; SOUZA, A. P.. |
The Guineagrass (Panicum maximum Jacq.) is one of the most important tropical forage grasses, but genetic knowledge and tools regarding this species are still limited. Therefore, 20 novel polymorphic microsatellite markers were developed, validated, and employed in estimating genetic relationships among 25 P. maximum genotypes selected from a Brazilian germplasm collection. In addition, they were tested for cross-species amplification in four other forage grass species. The number of alleles observed for each locus ranged from 4 to 12 (average 6.7). The values of polymorphism information content (PIC) varied from 0.41 to 0.83 (average 0.61) and the discriminating power (D) ranged from 0.53 to 0.98 (average 0.72). Cross-amplification demonstrated the... |
Tipo: Artigo de periódico |
Palavras-chave: Capim Tanzânia; Guineagrass; Marcador microssatélite; Amplificação cruzada; Fitomejoramiento; Repeticiones de microsatélite; Pastos forrajeros; Marcadores genéticos; Variación genética.; Melhoramento genético vegetal; Gramínea forrageira; Capim Colonião; Panicum maximum; Seleção genótipa; Variação genética; Marcador genético; Polimorfismo genético; Genótipo.; Plant breeding; Forage grasses; Megathyrsus maximus; Genotype; Genetic variation; Genetic markers; Microsatellite repeats; Genetic polymorphism.. |
Ano: 2011 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/901385 |
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PERSEGUINI, J. M. C. K.; CHIORATTO, A. F.; ZUCCHI, M. I.; COLOMBO, C. A.; CARBONELL, S. A. M.; MONDEGO, J. M. C.; GAZAFFI, R.; GARCIA, A. A. F.; CAMPOS, T. de; SOUZA, A. P. de; RUBIANO, L. B.. |
A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger?s modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were... |
Tipo: Artigo de periódico |
Palavras-chave: Marcador microssatélite; Feijão carioca; Frijoles; Variación genética.; Repeticiones de microsatélite; Marcadores genéticos; Fitomejoramiento; Melhoramento genético vegetal; Feijão; Phaseolus vulgaris; Variação genética; Marcador genético; Polimorfismo genético; Plant breeding; Beans; Genetic variation; Genetic markers; Microsatellite repeats; Genetic polymorphism.. |
Ano: 2011 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/902577 |
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