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| Registros recuperados: 45 | |
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| Gomes,Regina Lucia Ferreira; Costa,Marcones Ferreira; Alves-Pereira,Alessandro; Bajay,Miklos Maximiliano; Viana,João Paulo Gomes; Valente,Sérgio Emílio dos Santos; Lopes,Ângela Celis de Almeida; Zucchi,Maria Imaculada; Pinheiro,José Baldin. |
| ABSTRACT Some germplasm collections have a high number of accessions, which makes it difficult to explore the genetic variability present in the germplasm bank due to the redundancy and the difficulty of detailed analysis of all conserved accessions. Therefore, our study aimed to analyze the genetic diversity of 153 lima bean (Phaseolus lunatus) accessions for the purpose of constructing a core collection. Eleven SSRs were used for this purpose. The 153 lima bean accessions can be represented by low redundancy using a minimum of 34 accessions, thus representing 22 % of the size of the entire germplasm bank. The core collection had a higher Shannon diversity index and expected heterozygosity (1.906 and 0.811, respectively) than those presented by the entire... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Germplasm; Genetic diversity; Genetic structure; Microsatellite markers. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201100 |
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| Silvestre,Ellida de Aguiar; Costa,Márcio da Silva; Silva,Pollyana Oliveira da; Bajay,Miklos Maximiliano; Pinheiro,José Baldin; Zucchi,Maria Imaculada; Campelo,José Elivalto Guimarães; Britto,Fábio Barros. |
| The objective of this study was to characterize the genetic variability of Anglo-Nubian goats using microsatellite markers. The study was conducted using herds from four municipalities of Central-Northern Piauí (Teresina, José de Freitas, Campo Maior, and Angical), where technical information is scarce. Seven markers suggested by FAO were used (ILSTS11, McM527, INRA23, ETH10, OarfCB304, OarfCB48, and MAF209). The samples were genotyped using a 7% polyacrylamide gel. The average number of alleles per locus was 4.0, with observed and expected heterozygosity of 0.38 and 0.55, respectively. Few deviations from the Hardy-Weinberg equilibrium were observed for each population. Only two loci deviated significantly in two localities. The coefficient of gene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endogamy; Genetic structure; Microsatellite; Molecular markers. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015000400155 |
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| GALLARDO,M.H; GONZÁLEZ,C; MENA,C; LOMOVASKY,B; MORRICONI,E; CLASING,E. |
| The correspondence between allozymic variation and specific differentiation was studied in four populations corresponding to the three nominal, allopatric species of clam genus Eurhomalea (E. rufa, E. lenticularis, E. exalbida) described for southern South America. Allozyme variation scored in 12 loci was high as indicated by heterozygosity levels (15.8-20.7 %) and by the presence of only three monomorphic loci (Hk-2, Icd-2, and Xdh-1). These high estimates of allelic variability were influenced by the low levels of interspecific genetic similarity (I = 0.64) and for the high conspecific values of genetic identity observed. The high estimates of substructuring found at the species level (F ST = 0.39) contrasted with the low differentiation (F ST = 0.027)... |
| Tipo: Journal article |
Palavras-chave: Allozymic variation; Genetic structure; Taxonomic status. |
| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2003000300013 |
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| Leuzzi,Maria Sueli Papa; Almeida,Fernanda Simões de; Orsi,Mário Luís; Sodré,Leda Maria Koelblinger. |
| In this study, the RAPD technique was used to analyze the genetic structure of populations of the fish Astyanax altiparanae (Characidae, Tetragonopterinae) living in the lower, middle and upper Paranapanema River, Brazil. The aim was to assess this structure regarding fish handling and conservation programs. The genetic variability (P) was found to be 42.64%, 75% and 75% in the low, middle and upper reaches, respectively. The dendrogram of genetic similarity, obtained by comparative analysis of the sets of samples from the three sites, showed the formation of three clusters. All of the genetic parameters used indicate that the population in the lower Paranapanema is genetically different from those in the middle and upper sections. The theta P test shows... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Astyanax altiparanae; RAPD; Genetic structure; Genetic conservation; Fish. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300009 |
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| Borba,Tereza Cristina de Oliveira; Brondani,Rosana Pereira Vianello; Breseghello,Flávio; Coelho,Alexandre Siqueira Guedes; Mendonça,João Antônio; Rangel,Paulo Hideo Nakano; Brondani,Claudio. |
| Association analysis was applied to a panel of accessions of Embrapa Rice Core Collection (ERiCC) with 86 SSR and field data from two experiments. A clear subdivision between lowland and upland accessions was apparent, thereby indicating the presence of population structure. Thirty-two accessions with admixed ancestry were identified through structure analysis, these being discarded from association analysis, thus leaving 210 accessions subdivided into two panels. The association of yield and grain-quality traits with SSR was undertaken with a mixed linear model, with markers and subpopulation as fixed factors, and kinship matrix as a random factor. Eight markers from the two appraised panels showed significant association with four different traits,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Association analysis; Core collection; Genetic structure. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300023 |
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| Vendrami, David L. J.; Houston, Ross D.; Gharbi, Karim; Telesca, Luca; Gutierrez, Alejandro P.; Gurney-smith, Helen; Hasegawa, Natsuki; Boudry, Pierre; Hoffman, Joseph I.. |
| Cultivated bivalves are important not only because of their economic value, but also due to their impacts on natural ecosystems. The Pacific oyster (Crassostrea gigas) is the world's most heavily cultivated shellfish species and has been introduced to all continents except Antarctica for aquaculture. We therefore used a medium-density single nucleotide polymorphism (SNP) array to investigate the genetic structure of this species in Europe, where it was introduced during the 1960s and has since become a prolific invader of coastal ecosystems across the continent. We analyzed 21,499 polymorphic SNPs in 232 individuals from 23 localities spanning a latitudinal cline from Portugal to Norway and including the source populations of Japan and Canada. We confirmed... |
| Tipo: Text |
Palavras-chave: Aquaculture; Crassostrea gigas; Genetic structure; High-density genotyping array; Inbreeding; Pacific oyster; Restriction site-associated DNA (RAD) sequencing; Single nucleotide polymorphism (SNP). |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00484/59561/62557.pdf |
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| Perseguini,Juliana Morini Küpper Cardoso; Silva,Gliciane Micaele Borges; Rosa,João Ricardo Bachega Feijó; Gazaffi,Rodrigo; Marçal,Jéssica Fernanda; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando; Zucchi,Maria Imaculada; Garcia,Antonio Augusto Franco; Benchimol-Reis,Luciana Lasry. |
| Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; Genetic diversity; Genetic structure; Microsatellites; Phaseolus vulgaris L. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100067 |
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| Guzmán Valencia, Stephanie. |
| La identidad y estructura genética de poblaciones de Oligonychus punicae y O. perseae (Acari: Tetranychidae) fue estudiada en siete localidades productoras de aguacate Persea americana var. Hass. La identidad de los ácaros fue determinada morfológica y molecularmente mediante la secuenciación de una fracción del gen Citocromo Oxidasa subunidad I (COI). La variación genética intraespecífica de diferentes poblaciones de ambas especies de ácaros fue estudiada mediante la secuenciación de las regiones ITS1 e ITS2 del rADN. El análisis filogenético de las secuencias parciales del gen COI separó adecuadamente las dos especies de ácaros, confirmando los resultados de la identificación morfológica. El análisis de las secuencias de las regiones ITS1 e ITS2 del... |
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Palavras-chave: COI; ITS1; ITS2; Estructura genética; Entomología y Acarología; Maestría; Genetic structure. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1765 |
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| Sosa-Gomez,Daniel R.; Delpin,Katiaíres E.; Almeida,Alvaro M.R.; Hirose,Edson. |
| The Neotropical Brown Stink Bug (NBSB), Euschistus heros (Fabricius), has a wide distribution in Brazilian soybean fields, being more important in the central region of the country. The species is the main target of insecticide applications for stink bug pest control. We determined the variability among and within populations of the NBSB by random amplification of polymorphic DNA (RAPD) analysis. Samples of NBSB were collected in soybean fields from Ubiratã (PR), Londrina (PR), Centenário do Sul (PR), Cândido Mota (SP), Ponta Porã (MS) and Sapezal (MT). Genomic DNA was extracted from the head to minimize DNA contamination by endoparasites. DNA was amplified with 10 mer primers. Fifteen primers produced 246 bands. The genetic similarity matrix was obtained,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAPD; Molecular marker; Genetic structure; Gene flow. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000200009 |
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| Perseguini,Juliana Morini Küpper Cardoso; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Colombo,Carlos Augusto; Carbonell,Sérgio Augusto Moraes; Mondego,Jorge Mauricio Costa; Gazaffi,Rodrigo; Garcia,Antonio Augusto Franco; Campos,Tatiana de; Souza,Anete Pereira de; Rubiano,Luciana Benchimol. |
| A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLPs; Genetic structure; Genetic variability; Phaseolus vulgaris L.; SSRs. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017 |
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| Cardona,Christian Camilo Castañeda; Coronado,Yacenia Morillo; Conronado,Ana Cruz Morillo; Ochoa,Iván. |
| ABSTRACT The oil palm (Elaeis guineensis Jacq) plays an important role in the economy of some tropical countries; Colombia ranks first for production in Latin America and fifth in the world. The aim of this research was to characterize the genetic variability of 51 oil palm genotypes from the Congo with seven randomly amplified microsatellite markers RAM. As a result, 241 alleles were generated andthe number of polymorphic loci ranged from 46 to 14 for the ACA and CGA primers, respectively. High genetic diversity was found, with a total heterozygosity of 0.64, along with a high percentage of polymorphic loci, 89%. The coefficient of genetic differentiation (Fst) was 0.15, indicating that there was moderate genetic differentiation. The genetic diversity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breeding; Genetic markers; Germplasm; Genetic structure. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000400546 |
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| Soares,Sabrina Delgado; Bandeira,Ludmila Ferreira; Ribeiro,Stela Barros; Telles,Mariana Pires de Campos; Silva,João Augusto da; Borges,Canrobert Tormin; Coelho,Alexandre Siqueira Guedes; Novaes,Evandro. |
| Abstract Given its high-valued wood, the African mahogany (Khaya grandifoliola) has been envisaged as a renewable source of tropical hardwoods in Brazil. However, there are concerns about the hypothesized low diversity among the few K. grandifoliola germplasm sources introduced in the country. Using eight microsatellite markers, we evaluated the genetic diversity and divergence among 53 superior trees selected from three provenances of K. grandifoliola located in the state of Para. These populations are among the oldest plantations (>15 years) in Brazil and, therefore, the country's main seed sources. The average number of alleles per locus was 5.9, expected heterozygosity was moderate (^=0.56) and lower than the high observed heterozygosity (HO=0.74).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellites; SSR; Genetic structure; Genetic divergence; Hardwood. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400301 |
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| Registros recuperados: 45 | |
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