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A comparison of RAPD and ISSR markers reveals genetic diversity among sweet potato landraces (Ipomoea batatas (L.) Lam.) - doi: 10.4025/actasciagron.v34i2.12616 Agronomy
Moulin, Monique Moreira; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Rodrigues, Rosana; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Gonçalves, Leandro Simões Azeredo; Universidade Estadual do Norte Fluminense; Sudré, Cláudia Pombo; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Pereira, Messias Gonzaga; Universidade Estadual do Norte Fluminense Darcy Ribeiro.
Small farmers have played an important role in maintaining genetic resources of traditional varieties. Collecting and characterizing germplasm, especially landraces and heirloom varieties, are strategic activities that would create a knowledge base describing the traditional varieties of a given region and reduce the risk of genetic erosion. The objectives of this work were as follows: collect sweet potato roots from rural properties and local markets in Rio de Janeiro State, Brazil; characterize the accessions using RAPD and ISSR molecular markers, comparing the efficiency in discrimination of these two types of molecular markers; and estimate the genetic variability of the population. Fifty-nine samples from rural properties and 19 from local markets...
Palavras-chave: Genetic resources; Germplasm characterization; Genetic variability; Heirloom seeds.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12616
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A NEW WORLD MONKEY MICROSATELLITE (AP74) HIGHLY CONSERVED IN PRIMATES Acta biol.Colomb.
OKLANDER,LUCIANA INÉS; STEINBERG3,ELIANA RUTH; DOLORES MUDRY,MARTA.
Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Primates; Microsatellite markers; PCR amplification; Non-invasive sampling; Genomic conservation; Genetic variability.
Ano: 2012 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000100007
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A study of the diversity of whitefly-trnasmitted geminivirus in the Submedio of San Francisco Valley- Brazil using PCR. Repositório Alice
SILVEIRA, F. Q. de P.; ROCHA, H. G. C.; LIMA, M. F.; RESENDE, L. V.; AVILA, A. C. de; BEZERRA, I. C..
Suplemento. Edição dos Resumos do 31 Congresso Brasileiro de Fitopatologia, Fortaleza, 1998.
Tipo: Artigo de periódico Palavras-chave: Geminiviruws; Diversidade; Variabilidade genetica; Submedio Sao Francisco; White flies; Diversity; Genetic variability; Mosca Branca; Inseto Para Controle Biológico; Praga de Planta.
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/133384
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Aggressiveness between genetic groups I and II of isolates of Cercospora zeae-maydis Scientia Agricola
Mathioni,Sandra Marisa; Carvalho,; Brunelli,Kátia Regiane; Beló,André; Camargo,Luis Eduardo Aranha.
For many years, the gray leaf spot disease (GLS) caused by the fungus Cercospora zeae-maydis Tehon & Daniels, was not considered an important pathogen of maize (Zea mays, L.) in Brazil. However, the recent adoption of agronomical practices such as no-tillage and cultivation under central pivot irrigation systems increased the incidence and severity to the extent that GLS is now one of the most important diseases of maize. Isolates of C. zeae-maydis can be distinguished by two genetic groups (I and II) based on AFLP markers and on polymorphisms of the ITS and 5.8S rDNA regions. Until now, however, the biological implications of this distinction remain unclear. This study investigated whether isolates from the two genetic groups differ in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Disease resistance; Gray leaf spot; Genetic variability; Pathogenicity.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000600006
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Agro-morphological characterization and genetic diversity of Paullinia cupana var. sorbilis. Repositório Alice
OZORIO, P. R. da S.; ATROCH, A. L.; NASCIMENTO FILHO, F. J. do.
Caracterização morfoagronômica e diversidade genética entre cultivares de guaranazeiro: este trabalho objetivou caracterizar e estimar a diversidade genética entre dezoito cultivares de guaraná (Paullinia cupana var. sorbilis) com base em descritores morfoagronômicos, para identificar cultivares para uso em cruzamentos no programa de melhoramento genético.
Tipo: Artigo de periódico Palavras-chave: Genetic variability; Paullinia Cupana; Guaraná.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118222
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Agro-morphological characterization of upland rice accessions Scientia Agricola
Nascimento,Wellington Ferreira do; Silva,Edson Ferreira da; Veasey,Elizabeth Ann.
The agro-morphological characterization is fundamental in order to provide information for plant breeding programs. The aim of the present study was to characterize 146 accessions of upland rice (Oryza sativa L.), based on qualitative and quantitative agro-morphological descriptors. The experiment was conducted in Recife, state of Pernambuco, Brazil, using a randomized block design with three replicates. Polymorphism was observed among 12 of 14 qualitative characters evaluated, whereas significant differences (p < 0.05) were observed for 11 of the 14 analysed quantitative traits. Genetic variance was higher than environmental variance and the average inheritability coefficients were above 80 % for all characters, which ensures the predominance of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; Germplasm; Ex-situ conservation; Pre-breeding.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000600008
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Agronomic potential and selection of okra hybrids to obtain potential genitors Horticultura Brasileira
Maciel,Gabriel M; Finzi,Rafael R; Marra,Alexandre William C; Carvalho,Fábio J; Nogueira,Ana Paula O.
ABSTRACT Evaluation of pre-commercial hybrids in a germplasm bank is essential for determining its commercial potential or its utility as a potential genitor in a breeding program. The objective of this study was to determine genetic divergence and per se behavior of 47 pre-commercial hybrids from okra germplasm bank of the Universidade Federal de Uberlândia. Precocity index (%), number of fruits (fruits per plant), average fruit mass (g) and productivity (g per plant) were evaluated. Analysis of genetic divergence was performed by multivariate analysis using Mahalanobis distance with different clustering methods (UPGMA and canonical analysis). The performance of hybrids was compared by Scott-Knott (p= 0.05). A significant genetic variability among okra...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abelmoschus esculentus; Genetic variability; Heterosis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000100112
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Agrupamento de acessos e cultivares de três espécies de Brachiaria por RAPD - DOI: 10.4025/actasciagron.v30i4.5298 Agronomy
Ambiel, Ana Claudia; UNOESTE; Guaberto, Luciana Machado; UNOESTE; Vanderlei, Talita Marques; UNOESTE; Machado Neto, Nelson Barbosa; UNOESTE.
O propósito deste trabalho foi determinar a similaridade genética entre acessos de germoplasma e cultivares comerciais de três espécies de Brachiaria (inter e intraespecífica), por meio de marcadores RAPD. Sementes foram utilizadas como fonte de DNA. 10 “primers” decâmeros foram selecionados de 120 “primers” avaliados, produzindo 107 bandas polimórficas, as quais foram utilizadas para a análise de variância molecular (Amova), o coeficiente de similaridade de Jaccard e índices de fixação gênica. 24,40% da variabilidade genética total estão contidas entre as espécies e 75,60% dentro destas, com índice de fixação gênica (FST) 0,24. No dendrograma, houve a formação de dois ramos, um formado por P. maximum e B. arrecta e o outro subdividido em três: todas...
Palavras-chave: 5.02.01.03-4 Genética e Melhoramento Florestal Brachiaria; Marcadores moleculares; Variabilidade genética; Dendrograma; Índice de fixação gênica. Brachiaria; Molecular markers; Genetic variability; Dendrogram; Genetic fixation index.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5298
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Allozyme analysis of the four species of Hypostomus (Teleostei: Loricariidae) from the Ivaí river, upper Paraná river basin, Brazil - doi: 10.4025/actascibiolsci.v35i4.16355 Biological Sciences
Paiva, Suzana de; Universidade Estadual de Maringá; Zawadzki, Claudio Henrique; Universidade Estadual de Maringa; Ruvulo-Takasusuki, Maria Claudia Colla; Universidade Estadual de Maringá; Lapenta, Ana Silvia; Universidade Estadual de Maringá; Renesto, Erasmo; Universidade Estadual de Maringá.
Allozyme electrophoresis analysis were performed in four species of Hypostomus (Loricariidae), H. albopunctatus, H. hermanni, H. regani, e Hypostomus sp. 1/NUP 5612 from the Ivaí river, a tributary of the upper Paraná river. The study of 14 loci revealed diagnostic characters and exclusive alleles in a low frequency. The heterozygosity ranged from 0.000 in H. albopunctatus to 0.199 in H. hermanni, which was higher than the heterozygosity in other samples of Hypostomus in literature, as well as in other fish groups. Hypostomus albopunctatus and H. regani revealed higher similarity (I = 0.804), while H. hermanni and Hypostomus sp. 1/NUP 5612 showed the least genetic identity (I = 0.569). All samples were genetically distinguished, despite there were several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.02.00.00-5 catfish; Heterozygosity; Neotropical fishes; Genetic variability; Siluriformes Genética.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/16355
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Allozyme differentiation of two populations of the genus Neoplecostomus Eigenmann &amp; Eigenmann, 1888 (Teleostei, Loricariidae) from the upper Paraná River basin, Brazil Genet. Mol. Biol.
Reusing,Ana Flávia; Renesto,Erasmo; Roxo,Fábio F.; Zawadzki,Cláudio H..
Allozyme electrophoresis was used to examine 12 enzymatic systems in two populations of the genus Neoplecostomus from the Paraná River basin. Samples of Neoplecostomus sp. 1 were collected in Paraitinguinha stream of the Tietê River basin, in the municipality of Salesópolis, São Paulo State, and those of Neoplecostomus sp. 2 from São Domingos stream of the Rio Grande River basin, in the municipality of Muzambinho, Minas Gerais State. The genetic variability of the two populations was estimated by Nei's expected heterozygosity and was considered lower than average for populations of freshwater fish. The proportion of polymorphic loci was low (only 5.26% for the locus Idh). The low frequency of heterozygosity for both populations revealed a high fixation of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Genetic variability; Neoplecostominae; Neotropical fishes.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300021
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Allozyme variation among three populations of the armored catfish Hypostomus regani (Ihering, 1905) (Siluriformes, Loricariidae) from the Paraná and Paraguay river basins, Brazil Genet. Mol. Biol.
Zawadzki,Cláudio H.; Renesto,Erasmo; Peres,Maria Dolores; Paiva,Suzana.
Three Brazilian populations of the armored catfish Hypostomus regani (Ihering, 1905) were sampled, one from the Corumbá Reservoir in Goiás state, another from the Itaipu Reservoir in Paraná state and a third from the Manso Reservoir in Mato Grosso state. Allozyme electrophoresis was used to establish the genetic structure of the species, with the analysis of liver, heart and muscles tissues allowing the scoring of 25 loci from 14 enzymatic systems. Although no diagnostic loci were found, some exclusive rare alleles were recorded for the three populations. The genetically most similar populations were those from Corumbá and Itaipu, and the most distant were the populations from Manso and Corumbá. The allozyme data showed three structured populations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Genetic variability; Hypostomus regani; Loricariidae; Paraguay and Paraná Rivers.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400025
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Análise da variabilidade genética de populações de Spdoptera frugiperda (lepidoptera:Noctuidae) em culturas de milho e algodão no Brasil. Repositório Alice
RAMIRO, C. A.; QUEIROZ, P. R.; MONNERAT, R. G..
Tipo: Anais e Proceedings de eventos Palavras-chave: Insect; Variabilidade genética; Genetic variability; Brasil.; Algodão; Controle Biológico; Lagarta; Milho; Praga; Spodoptera Frugiperda.; Cotton; Corn; Biological control; Brazil; Pests..
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/188667
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Análise discriminante das características físicas e químicas de frutos de pupunha (Bactris gasipaes Kunth) do alto Rio Madeira, Rondônia, Brasil. Repositório Alice
SANTOS, B. W. C. dos; FERREIRA, F. M.; SOUZA, V. F. de; CLEMENT; ROCHA, R. B..
O objetivo deste trabalho foi caracterizar as propriedades físicas e químicas dos frutos de pupunha visando a quantificar a variabilidade genética e a associação dos caracteres com a classificação tradicional estabelecida pela massa dos frutos [microcarpa (10 a 20 g), mesocarpa (20 a 70 g) e macrocarpa (>70 g)].
Tipo: Separatas Palavras-chave: Bromatology; Genetic variability; Melhoramento de plantas; Variabilidade genética.; Bromatologia.; Plant breeding..
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1087180
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Analysis of diversity among six populations of Colombian mango (Mangifera indica L. cvar. Hilacha) using RAPDs markers Electron. J. Biotechnol.
Díaz-Matallana,Marcela; Schuler-García,Ingrid; Ruiz-García,Manuel; Hodson de Jaramillo,Elizabeth.
Currently in Colombia, there are only records of morph-agronomic characterizations of Mangifera indica cvar. Hilacha; molecular studies on this mango variety have not been carried out. The aim of this work was to identify the genetic diversity of six populations of mango Hilacha by RAPDs markers, as a fundamental base for breeding programs, conservation and selection of promissory materials for the fruit industry at the national level. From 60 primers evaluated in the populations, five primers were selected and were launched in the six populations. Polymorphic bands of RAPDs were transformed into binary matrices, which were then processed with NTSYS-PC, POPGENE and TFPGA softwares. The overall genetic diversity, H T = 0.468 ± 0.0016, is very similar to the...
Tipo: Journal article Palavras-chave: Genetic variability; Mango Hilacha; Organoleptic quality; RAPDs.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300001
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Analysis of genetic variability of commercial melon cultivars using SSR molecular markers. Repositório Alice
CARVALHO, N.; CANELA, F. M.; LEITE, P. H. S.; FERREIRA, M. A.; OLIVEIRA, V. R.; SANTOS, M. F.; SOUZA, N. O. S.; BUSO, G. S. C..
Tipo: Separatas Palavras-chave: Commercial cultivars; SSR; Molecular markers.; Genetic variability; Melão; Marcador molecular; Cucumis melo.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079736
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Analysis of phenotypes altered by temperature stress and hipermutability in Drosophila willistoni Iheringia, Sér. Zool.
D'Ávila,Marícia F.; Garcia,Rosane N.; Loreto,Elgion L. S.; Valente,Vera Lúcia da S..
Drosophila willistoni (Sturtevant, 1916) is a species of the willistoni group of Drosophila having wide distribution from the South of USA (Florida) and Mexico to the North of Argentina. It has been subject of many evolutionary studies within the group, due to its considerable ability to successfully occupy a wide range of environments and also because of its great genetic variability expressed by different markers. The D. willistoni 17A2 strain was collected in 1991 in the state of Rio Grande do Sul, Brazil (30°05'S, 51°39'W), and has been maintained since then at the Drosophila laboratory of UFRGS. Different to the other D. willistoni strains maintained in the laboratory, the 17A2 strain spontaneously produced mutant males white-like (white eyes) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: D. willistoni; 17A2 strain; Temperature stress; Genetic variability; Hypermutability.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212008000300009
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Aplicação de marcadores moleculares no melhoramento genético. Repositório Alice
LANZA, M. A.; GUIMARAES, C. T.; SCHUSTER, I..
O melhoramento genetico tem sido um dos grandes responsaveis pelos avancos na agricultura, com o desenvolvimento de cultivares superiores, quer pela maior produtividade, quer pela melhor adaptacao aos ambientes adversos. O sucesso de um programa de melhoramento genetico depende fundamentalmente de algumas etapas como a escolha de genitores que produzam individuos com a melhor combinacao de alelos favoraveis e a selecao de genotipos superiores em populacoes segregantes. Uma potente ferramenta para auxiliar o melhoramento sao os marcadores moleculares, que fornecem um numero ilimitado de polimorfismos com base do DNA e sao independentes dos efeitos ambientais e do estadio fisiologico da planta, permitindo a identificacao precoce e precisa de individuos com...
Tipo: Artigo de periódico Palavras-chave: Alelos; Variabilidade genetica; Genetic variability; Molecular marker.; Marcador Molecular; Polimorfismo.; Genome..
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/484109
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Assessment of Genetic Variability of Fish Personality Traits using Rainbow Trout Isogenic Lines ArchiMer
Millot, Sandie; Pean, Samuel; Labbe, Laurent; Kerneis, Thierry; Quillet, Edwige; Dupont-nivet, Mathilde; Begout, Marie-laure.
The study of inter-individual variability of personality in fish is a growing field of interest but the genetic basis of this complex trait is still poorly investigated due to the difficulty in controlling fish genetic origin and life history. When available, isogenic lines that allow performing independent tests on different individuals having identical genotype constitute a very relevant experimental material to disentangle the genetic and environmental components of behavioural individuality. We took advantage of heterozygous isogenic lines to investigate the personality in rainbow trout through the analysis of their reactions to different experimental situations. To this end, seven to ten rainbow trout isogenic lines were screened for their spatial...
Tipo: Text Palavras-chave: Personality; Isogenic lines; Genetic variability; Risk taking; Spatial exploration; Oncorhynchus mykiss.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00194/30496/29976.pdf
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Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers Genet. Mol. Biol.
Silveira,Sheila Recepute; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Sera,Tumoru; Carvalho,Valdemar de Paula; Coelho,Alexandre Siqueira Guedes.
The RAPD technique associated with restriction digestion of genomic DNA was used to assess the genetic variability within and among nine populations of Coffea arabica, including six progenies belonging to the Sarchimor germplasm, the progeny PR 77054-40-10 (Catuaí Vermelho IAC 81 x Icatu), and two commercial cultivars (IAPAR 59 and Catuaí Vermelho IAC-81). These populations were evaluated using analysis of molecular variance (AMOVA), genetic similarity among progenies, and percentage of polymorphic loci. A total of 99 RAPD markers were evaluated of which 67 (67.67%) were polymorphic. AMOVA showed that 38.5% and 61.5% of the genetic variation was distributed among and within populations, respectively. The fixation index (F ST) of the genotypes was 0.385....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee breeding; Genetic variability; Molecular variance; RAPD markers.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300018
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Association between BMP4 gene polymorphism and in vitro embryo production traits in Gyr cows. Repositório Alice
ORTIZ, W. H.; QUIRINO, C. R.; SILVA, A.; OLIVEIRA, C. S.; SERAPIÃO, R. V.; PACHECO, A.; BARTHOLAZZI, A..
Tipo: Separatas Palavras-chave: Genetic variability; Molecular markers; OPU-IVP; SNPs.; Mutation..
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035211
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