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Identification and validation of novel EST-SSR markers in olives Scientia Agricola
Arbeiter,Alenka Baruca; Hladnik,Matjaž; Jakše,Jernej; Bandelj,Dunja.
ABSTRACT: The olive (Olea europaea L.) is a leading oil crop in the Mediterranean area. Limited information on the inheritance of agronomic significant traits hinders progress in olive breeding programs, which encourages the development of markers linked to the traits. In this study, we report on the development of 46 olive simple sequence repeat (SSR) markers, obtained from 577,025 expressed sequence tags (ESTs) in developing olive fruits generated in the framework of the Slovenian national olive transcriptome project. Sequences were de novo assembled into 98,924 unigenes, which were then used as a source for microsatellites searching. We identified 923 unigenes that contained 984 SSRs among which dinucleotide SSRs (36 %) were the most abundant, followed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Expressed sequence tags; Microsatellites; Genic markers; Genetic diversity; Functional annotation.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000300215
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