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Contents and bioconversion of B-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh. Repositório Alice
BORGES, C. W. C.; CARRÃO-PANIZZI, M. C.; MANDARINO, J. M. G.; SILVA, J. B. da; BENEDETTI, S.; IDA, E. I..
The objective of this work was to evaluate the effect of the processing conditions of soybean tempeh on the contents of ??glycoside isoflavones and on their bioconversion into aglycones. Different times of soaking (6, 12, and 18 hours), cooking (15, 30, and 45 minutes), and fermentation (18, 24, and 30 hours) with Rhizopus oligosporus at 37°C were evaluated for tempeh preparation. Grains from the cultivar 'BRS 267' were used, and the experiment was carried out according to a central composite design (23). The response functions comprised the contents of genistin, malonyldaidzin, malonylgenistin, daidzein, and genistein, quantified by ultraperformance liquid chromatography (UPLC). Soaking, cooking, and fermentation times change the content, profile, and...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Alimento funcional; Daidzeína; Genisteína; Soja; Soja fermentada; Fermented soybean; Glycine max; Rhizopus microsporus var oligosporus; Glycine max; Daidzein; Functional foods; Genistein.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1049699
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Contents and bioconversion of B-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh. Repositório Alice
BORGES, C. W. C.; CARRÃO-PANIZZI, M. C.; MANDARINO, J. M. G.; SILVA, J. B. da; BENEDETTI, S.; IDA, E. I..
ABSTRACT: The objective of this work was to evaluate the effect of the processing conditions of soybean tempeh on the contents of β‑glycoside isoflavones and on their bioconversion into aglycones. Different times of soaking (6, 12, and 18 hours), cooking (15, 30, and 45 minutes), and fermentation (18, 24, and 30 hours) with Rhizopus oligosporus at 37°C were evaluated for tempeh preparation. Grains from the cultivar 'BRS 267' were used, and the experiment was carried out according to a central composite design (23). The response functions comprised the contents of genistin, malonyldaidzin, malonylgenistin, daidzein, and genistein, quantified by ultraperformance liquid chromatography (UPLC). Soaking, cooking, and fermentation times change...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Soja; Daidzeína; Soja fermentada; Alimento funcional; Genisteína; Soja; Nutrição humana; Rhizopus; Fungo; Alimento fermentado; Soybeans; Human nutrition; Daidzein; Fermented foods; Functional foods; Genistein.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1044976
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Contents and bioconversion of β-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh PAB
Borges,Cristiane Wing Chong; Carrão-Panizzi,Mercedes Concórdia; Mandarino,José Marcos Gontijo; Silva,Josemeyre Bonifácio da; Benedetti,Silvia; Ida,Elza Iouko.
Abstract: The objective of this work was to evaluate the effect of the processing conditions of soybean tempeh on the contents of β-glycoside isoflavones and on their bioconversion into aglycones. Different times of soaking (6, 12, and 18 hours), cooking (15, 30, and 45 minutes), and fermentation (18, 24, and 30 hours) with Rhizopus oligosporus at 37°C were evaluated for tempeh preparation. Grains from the cultivar 'BRS 267' were used, and the experiment was carried out according to a central composite design (23). The response functions comprised the contents of genistin, malonyldaidzin, malonylgenistin, daidzein, and genistein, quantified by ultraperformance liquid chromatography (UPLC). Soaking, cooking, and fermentation times change the content,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Rhizopus oligosporus; Daidzein; Fermented soybean; Functional food; Genistein.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000300271
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Disruption of reproductive development in male rat offspring following gestational and lactational exposure to di-(2-ethylhexyl) phthalate and genistein Biol. Res.
Zhang,Lian-Dong; Deng,Qian; Wang,Zi-Ming; Gao,Ming; Wang,Lei; Chong,Tie; Li,He-Cheng.
Studies of developmental effects of mixtures of endocrine disrupters on the male reproductive system are of great concern. In this study, the reproductive effects of the co-administration of di-2-(ethylhexyl) phthalate (DEHP) and genistein (GEN) during pregnancy and lactation were studied in male rat offspring. Pregnant Sprague-Dawley rats were gavaged from gestation day 3 to postnatal day 21 with vehicle control, DEHP 250 mg/kg body weight (bwyday, GEN 50 mg/kg bwday, GEN 400 mg/kg bwday, and two combinations of the two compounds (DEHP 250 mg/kg bwday + GEN 50 mg/kg bwday, DEHP 250 mg/kg bwday + GEN 400 mg/kg bwday). The outcomes studied were general morphometry (weight, AGD), testicular histology, testosterone levels, and expression at the mRNA level of...
Tipo: Journal article Palavras-chave: Di-2-(ethylhexyl) phthalate; Genistein; Pregnancy; Lactation; Reproductive system.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200004
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Effect of genistein-enriched diets on the endocrine process of gametogenesis and on reproduction efficiency of the rainbow trout Oncorhynchus mykiss ArchiMer
Bennetau Pelissero, Catherine; Breton, Bernard; Bennetau, Bernard; Corraze, Genevieve; Le Menn, Françoise; Davail Cuisset, Blandine; Helou, Chantal; Kaushik, Sadasivam.
Three practical diets were formulated to contain 0, 500, or 1000 ppm genistein. The three diets were distributed for 1 year to groups of rainbow trout undergoing their first gametogenesis and until spawning. Growth performance of rainbow trout was not affected by dietary treatments. Plasma cholesterol levels were equivalent between groups. In males, a slight but constant induction of vitellogenin (VTG) synthesis and a decrease in testosterone levels were observed. A slight decrease in plasma levels of beta FSH and beta LH was noticed at the end of spermatogenesis in the male fish fed a diet with 500 ppm (genistein) (from 2.16 +/- 0.39 to 1.47 +/- 0.23 for beta FSH and from 0.44 +/- 0.09 to 0.31 +/- 0.09 for beta LH). There was a significantly reduced 17...
Tipo: Text Palavras-chave: Diet; Reproduction; GTH; Vitellogenin; Steroid; Trout; Genistein.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-738.pdf
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Effect of storage period on isoflavone content and physiological quality of conventional and transgenic soybean seeds Rev. bras. sementes
Ávila,Marizangela Rizzatti; Braccini,Alessandro de Lucca; Albrecht,Leandro Paiola; Scapim,Carlos Alberto; Mandarino,José Marcos Gontijo; Bazo,Gabriel Loli; Cabral,Yara Camila Fabrin.
The objective in this research was to evaluate the isoflavone content and the physiological quality of seed from conventional and transgenic soybean cultivars before and after 180 days of storage. Twenty one soybean cultivars: CD 202, CD 206, CD 208, CD 213RR, CD 214RR, CD 215, CD 216, CD 217, CD 218, CD 221, BRS 184, BRS 185, BRS 214, BRS 244RR, BRS 245RR, BRS 246RR, BRS 255, BRS 257, BRS 258, BRS 261 and BRS 262, grown in the 2005/2006 crop season, were assayed. The seeds were packed in Kraft paper bags and stored at room temperature under laboratory conditions. Seeds were evaluated with respect to their germination and vigor (first germination count, accelerated aging and tetrazolium test) and their total isoflavone contents and respective aglycon forms...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aglycon; Glycine max; Daidzein; Genistein; Vigour.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222011000100017
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Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats Biol. Res.
AJDŽANOVI,VLADIMIR Z; ŠOŠI-JURJEVI,BRANKA T; FILIPÓVI,BRANKO R; TRIFUNOVI,SVETLANA L; BRKIC,DEJAN D; SEKULI,MILKA I; MILOSEVI,VERICA LJ.
The soybean phytoestrogen, genistein, is increasingly consumed as an alternative therapeutic for age-related diseases, namely cardiovascular conditions, cancer and osteoporosis. However, despite the beneficial effects on health, concern has been raised that this isoflavone also acts as an endocrine-disrupting chemical. The aim of this study was to examine the effects of genistein on immunohistomorphometric features of pituitary adrenocorticotropic cells (ACTH) and blood concentrations of ACTH and corticosterone in orchidectomized middle-aged male rats. Sixteen-month-old Wistar rats were divided into sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30mg/kg/day) was administered subcutaneously for...
Tipo: Journal article Palavras-chave: ACTH cells; Corticosterone; Genistein; Middle-age; Orchidectomy; Rats.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100002
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Interruption of the blood-stage cycle of the malaria parasite, Plasmodium chabaudi, by protein tyrosine kinase inhibitors BJMBR
Gazarini,M.L.; Garcia,C.R.S..
Malaria is a devastating disease caused by a unicellular protozoan, Plasmodium, which affects 3.7 million people every year. Resistance of the parasite to classical treatments such as chloroquine requires the development of new drugs. To gain insight into the mechanisms that control Plasmodium cell cycle, we have examined the effects of kinase inhibitors on the blood-stage cycle of the rodent malaria parasite, Plasmodium chabaudi. In vitro incubation of red blood cells for 17 h at 37ºC with the inhibitors led to a decrease in the percent of infected cells, compared to control treatment, as follows: genistein (200 µM - 75%), staurosporine (1 µM - 58%), R03 (1 µM - 75%), and tyrphostins B44 (100 µM - 66%) and B46 (100 µM - 68%). All these treatments were...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Malaria; Plasmodium chabaudi; Kinase inhibitors; Signal transduction; Genistein; Staurosporin.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100003
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Involvement of a Novel Genistein-Inducible Multidrug Efflux Pump of Bradyrhizobium japonicum Early in the Interaction with Glycine max (L.) Merr OAK
Takeshima, Keisuke; Hidaka, Tatsuo; Min Wei2); Yokoyama, Tadashi; Minamisawa, Kiwamu; Mitsui, Hisayuki; Itakura, Manabu; Kaneko, Takakazu; Tabata, Satoshi; Saeki, Kazuhiko; Oomori, Hirofumi; Tajima, Shigeyuki; Uchiumi, Toshiki; Abe, Mikiko; Tokuji, Yoshihiko; Ohwada, Takuji; 得字, 圭彦; 大和田, 琢二.
The early molecular dialogue between soybean and the bacterium Bradyrhizobium japonicum is crucial for triggering their symbiotic interaction. Here we found a single large genomic locus that is widely separated from the symbiosis island and was conspicuously induced within minutes after the addition of genistein. This locus (named BjG30) contains genes for the multidrug efflux pump, TetR family transcriptional regulator, and polyhydroxybutyrate (PHB) metabolism. The induction of BjG30 by genistein was competitively inhibited by daidzein, although both genistein and daidzein are soybean-derived inducers of nodulation (nod) genes. Such a differential expression pattern is also observed in some legume-derived flavonoids, which structurally differ in the...
Palavras-chave: Bradyrhizobium; Genistein; Multidrug efflux pump; TetR family transcriptional regulator.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3945
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The use of HPLC identification and quantification of isoflavones content in samples obtained in pharmacies - doi: 10.4025/actascibiolsci.v33i1.6876 Biological Sciences
Mantovani, Daniel; Universidade Federal do Paraná; Cardozo Filho, Lúcio; Universidade Estadual de Maringá; Santos, Leonilda Correia; Unversidade Estadual do Oeste do Paraná; Souza, Vera Lúcia Ferreira de; Universidade Estadual de Maringá; Watanabe, Cecilia Sumie; Universidade Estadual de Maringá.
Nowadays, there is a great interest in the research and the production of functional foods, such as isoflavones. Which present proven action on the prevention of health problems, such as cancer and cardiovascular diseases. Considering the importance of soybean supplements standardization, this study aimed identifying and quantifying the isoflavones in products sold at different pharmacies. Isoflavones samples were acquired in six different pharmacies specialized in the production of phytotherapic medications. The isoflavones were extracted, quantified and identified in HPLC. None of the samples, from different pharmacies, presented the isoflavones content presented on the label, i.e., 20 mg. In relation to the isoflavones profile, the highest levels found...
Palavras-chave: 2.08.00.00-2 Daidzein; Genistein; Functional compounds; Soybean; Quantity bioquimica Daidzein; Genistein; Functional compounds; Soybean; Quantity.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6876
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