|
|
|
| Registros recuperados: 42 | |
|
|
| Li,Biaoru. |
| Recent advances in functional genomics allow us to estimate the expression of several thousands of genes in the mammalian genome. Techniques such as microarrays, expressed tag sequencing (EST), serial analysis of gene expression (SAGE), subtractive cloning and differential display (DD), and two-dimensional electrophoresis gel have been extensively used to screen and analyze parallel gene expression. Some pathological processes, for example, tumorigenesis and solid tumour growth, in which the former is derived from a single-cell and the latter has a mixed-cell problem, present new challenges to the limit of these functional genomic techniques. To fully understand the functions of cells in tumorigenesis or in heterogeneous solid tumour masses, it is... |
| Tipo: Journal article |
Palavras-chave: Genome; Genomic expression; Genomic expression analysis at single cell; Single cell. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100011 |
| |
|
|
| Rekaya,Romdhane; Robbins,Kelly. |
| In recent years there has been much focus on the use of single nucleotide polymorphism (SNP) fine genome mapping to identify causative mutations for traits of interest; however, many studies focus only on the marginal effects of markers, ignoring potential gene interactions. Simulation studies have show that this approach may not be powerful enough to detect important loci when gene interactions are present. While several studies have examined potential gene interaction, they tend to focus on a small number of SNP markers. Given the prohibitive computation cost of modeling interactions in studies involving a large number SNP, methods need to be develop that can account for potential gene interactions in a computationally efficient manner. This study adopts... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genome; Simulation; SNP. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300011 |
| |
|
|
| Brandão,José Henrique S. G.; Rodrigues,Nureyev F.; Eguiluz,Maria; Guzman,Frank; Margis,Rogerio. |
| Abstract Araucaria angustifolia is endemic to southern Brazil. Known as Brazilian pine, A. angustifolia is the only native conifer species with economic and social relevance in this country. Due to massive exploitation, it has suffered a significant population decline and currently is classified as critically endangered. This encouraged the scientific community to investigate genetic features in Brazilian pine to increase resources for management and preservation. In this work, RNA-Seq data was used to determine the complete nucleotide sequence of the A. angustifolia chloroplast genome (cpDNA). The cpDNA is 146,203 bp in length and contains 122 genes, including 80 protein-coding genes, 5 ribosomal RNA genes, and 37 tRNA genes. Coding regions comprise... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian pine; Plastid; Genome; CpDNA; Conservation. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400671 |
| |
|
|
| Rodríguez Yunta,Eduardo; Valdebenito Herrera,Carolina; Misseroni,Adelio; Fernández Milla,Lautaro; Outomuro,Delia; Schiattino Lemus,Irene; Ferrer Lues,Marcela; Lolas Stepke,Fernando. |
| The present reflection refers to data obtained about the social representations of genomic research and its applications through interviews with legislators and lawyers, biomedical researchers and civilians and the review of scientific and legal literature in four Latin American countries: Argentine, Chile, Mexico and Peru. Several issues are addressed: little access to prevention and therapeutic methods, lack of equity in health benefits, commercialization of gene sequences through patents which leads to commercial exploitation of underdeveloped countries, the possibility of physical or psychological damage or genetic discrimination, the possibility of genetic modifications or abortion for eugenic reasons, the necessity of safeguarding confidentiality,... |
| Tipo: Journal article |
Palavras-chave: Cloning; Genome; Social representations; Transgenic. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300003 |
| |
|
| |
|
|
| Vianna,Juliana A.; Noll,Daly; Mura-Jornet,Isidora; Valenzuela-Guerra,Paulina; González-Acuña,Daniel; Navarro,Cristell; Loyola,David E.; Dantas,Gisele P. M.. |
| Abstract Microsatellites are valuable molecular markers for evolutionary and ecological studies. Next generation sequencing is responsible for the increasing number of microsatellites for non-model species. Penguins of the Pygoscelis genus are comprised of three species: Adélie (P. adeliae), Chinstrap (P. antarcticus) and Gentoo penguin (P. papua), all distributed around Antarctica and the sub-Antarctic. The species have been affected differently by climate change, and the use of microsatellite markers will be crucial to monitor population dynamics. We characterized a large set of genome-wide microsatellites and evaluated polymorphisms in all three species. SOLiD reads were generated from the libraries of each species, identifying a large amount of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellite markers; Penguin; Genome; Polymorphism; Tetranucleotide. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400676 |
| |
|
| |
|
|
| Ren, Weicheng; Chen, Haixia; Renault, Tristan; Cai, Yuyong; Bai, Changming; Wang, Chongming; Huang, Jie. |
| Acute viral necrosis virus (AVNV) is the causative agent of a serious disease resulting in high mortality in cultured Chinese scallops, Chlamys farreri. We have sequenced and analyzed the complete genome of AVNV. The AVNV genome is a linear, double-stranded DNA molecule of 210,993 bp with a nucleotide composition of 38.5% G + C. A total of 123 open reading frames were predicted to encode functional proteins, ranging from 41 to 1,878 amino acid residues. The DNA sequence of AVNV is 97% identical to that of ostreid herpesvirus 1 (OsHV-1), and the amino acid sequences of the encoded proteins of these two viruses are 94-100% identical. The genomic organization of AVNV is similar to that of OsHV-1, and consists of two unique regions (170.4 kb and 3.4 kb,... |
| Tipo: Text |
Palavras-chave: Acute viral necrosis virus; Herpesvirus; OsHV-1; Genome; Scallop; Chlamys farreri; AVNV. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00135/24620/22650.pdf |
| |
|
| |
|
|
| Alcántara Mendoza, Susana. |
| Una nueva enfermedad del maíz apareció en el estado de Veracruz, México durante 2003-2004. Síntomas de amarillamiento, enrojecimiento y posterior quemado de hojas, proliferación de mazorcas, jilotes raquíticos, falta de fecundación y granos vanos fueron parte del síndrome de la enfermedad. El fitoplasma Maize Bushy Stunt (MBS) se identifico por PCR se detectó en plantas con los síntomas antes descritos, en los municipios de Tlalixcoyan, Cosoleacaque y Paso de Ovejas en 2006 y 2007. Los genotipos CP-562, 30F83, 30F92, Orca, 30F96, Nutria y Asgrow 7573 resultaron positivos a la presencia del MBS, mientras que en 3086, 30F97 y CP-560 no se detectó la presencia del MBS en una plantación experimental en plantas sintomáticas en Tlalixcoyan, Ver. en 2006 y 2007.... |
|
Palavras-chave: Maize Bushy Stunt; PCR; Incidencia; Resistencia genética; Genoma; Homología; Incidence; Genetic resistance; Genome; Homology; Maestría en Ciencias; Postgrado en Fitosanidad; Fitopatología. |
| Ano: 2009 |
URL: http://hdl.handle.net/10521/53 |
| |
|
|
| Alcántara Mendoza, Susana. |
| Una nueva enfermedad del maíz apareció en el estado de Veracruz, México durante 2003-2004. Síntomas de amarillamiento, enrojecimiento y posterior quemado de hojas, proliferación de mazorcas, jilotes raquíticos, falta de fecundación y granos vanos fueron parte del síndrome de la enfermedad. El fitoplasma Maize Bushy Stunt (MBS) se identifico por PCR se detectó en plantas con los síntomas antes descritos, en los municipios de Tlalixcoyan, Cosoleacaque y Paso de Ovejas en 2006 y 2007. Los genotipos CP-562, 30F83, 30F92, Orca, 30F96, Nutria y Asgrow 7573 resultaron positivos a la presencia del MBS, mientras que en 3086, 30F97 y CP-560 no se detectó la presencia del MBS en una plantación experimental en plantas sintomáticas en Tlalixcoyan, Ver. en 2006 y 2007.... |
|
Palavras-chave: Maize Bushy Stunt; PCR; Incidencia; Resistencia genética; Genoma; Homología; Incidence; Genetic resistance; Genome; Homology; Maestría; Postgrado en Fitosanidad; Fitopatología. |
| Ano: 2009 |
URL: http://hdl.handle.net/10521/53 |
| |
|
|
| Škraban,Jure; Kyrpides,Nikos C.; Shapiro,Nicole; Whitman,William B.; Trček,Janja. |
| ABSTRACT The type strain SUR2 of the novel species Chryseobacterium limigenitum was isolated from a dehydrated sludge of the municipal sewage treatment plant in Dogoše near Maribor in Slovenia. The draft genome, with 60 contigs, 4,697,725 bp, 34.4% of G+C content, was obtained using the Illumina HiSeq 2500-1 platform. Joint Genome Institute Microbial Genome Annotation Pipeline (MGAP v.4) has identified 4322 protein-coding sequences including resistance genes against arsenic and other heavy metals. In addition, a subclass B3 metallo-β-lactamase, which confers resistance to penicillins, cephalosporins and carbapenems, was also present in the genome. The genome sequence provides important information regarding bioremediation potential and pathogenic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chryseobacterium limigenitum; Genome; Arsenite; Lactamase. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100005 |
| |
|
| |
|
|
| Carrier, Gregory; Baroukh, Caroline; Rouxel, Catherine; Duboscq-bidot, Laetitia; Schreiber, Nathalie; Bougaran, Gael. |
| Tisochrysis lutea is a microalga species currently used in aquaculture as a feed for shellfish, oysters and shrimps. It also has many other potential industrial applications, such as the production of neutral lipids for biofuels or the production of ω-3 fatty acids for nutraceuticals (human food complements). To efficiently exploit the potential of this microalga, however, higher lipid productivities are needed. To this end, improvement programs need to be developed and optimized. The diversity of strains available in microalgae has not yet been exploited in such improvement programs. In this study, the intra-strain diversity was observed and exploited to increase neutral lipid productivity. New clonal strains with higher neutral lipid productivity were... |
| Tipo: Text |
Palavras-chave: Microalgae; Genome; Improvement program; Lipids; Tisochrysis lutea. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00411/52204/52927.pdf |
| |
|
|
| Peroni,Luis Antonio; Ferreira,Renato Rodrigues; Figueira,Antonio; Machado,Marcos Antonio; Stach-Machado,Dagmar Ruth. |
| Plants not only evolve but also reduce oxygen in photosynthesis. An inevitable consequence of this normal process is the production of reactive oxygen species (ROS). Plants are adequately protected by the presence of multiple antioxidative enzymes in the cytosol and also in the different cell organelles such as chloroplasts, mitochondria, and peroxisomes. Traditionally, ROS were considered to be only a toxic byproduct of aerobic metabolism. However, recently it has become apparent that plants actively produce these molecules which may control many different physiological processes such as abiotic and biotic stress response, pathogen defense and systemic signaling. The search results using the Citrus Genome Program in Brazil (CitEST) for oxidative stress... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Genome; EST; Reactive oxygen species; Oxidative stress enzyme. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500016 |
| |
|
|
| Carazzolle,Marcelo F.; Formighieri,Eduardo F.; Digiampietri,Luciano A.; Araujo,Marcos R.R.; Costa,Gustavo G.L.; Pereira,Gonçalo A.G.. |
| Genome projects, both genomic DNA and ESTs (cDNA), generate a large amount of information, demanding time and a well-structured bioinformatics laboratory to manage these data. These genome projects use information available in heterogeneous formats from different sources. The amount and heterogeneity of this information, as well as the absence of a world consensus pattern, make the integration of these data a difficult task. At the same time, sub-tasks, such as microarray analyses of these projects, are very complex. This creates a demand for the development of creative solutions for ongoing annotation, thematic projects, microarray experiments, etc. This paper presents Gene Projects, a system developed to integrate all kinds of solutions. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Annotation; Genome; Web; Microarray and clustering. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500032 |
| |
|
|
| Santos,Mônica de Oliveira; Castro,Nadya da Silva; Pereira,Maristela; Soares,Célia Maria de Almeida. |
| This is a report on the analysis of genes involved in translation of the complete genomes of Mycoplasma hyopneumoniae strain J and 7448 and Mycoplasma synoviae. In both genomes 31 ORFs encoding large ribosomal subunit proteins and 19 ORFs encoding small ribosomal subunit proteins were found. Ten ribosomal protein gene clusters encoding 42 ribosomal proteins were found in M. synoviae, while 8 clusters encoding 39 ribosomal proteins were found in both M. hyopneumoniae strains. The L33 gene of the M. hyopneumoniae strain 7448 presented two copies in different locations. The genes encoding initiation factors (IF-1, IF-2 and IF-3), elongation factors (EF-G, EF-Tu, EF-Ts and EF-P), and the genes encoding the ribosome recycling factor (frr) and one polypeptide... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasmas; Mycoplasma synoviae; Mycoplasma hyopneumoniae; Translation; Genome. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200010 |
| |
|
|
| Zhang,Hai-Qin; Yang,Rui-Wu; Zhang,Li; Ding,Chun-Bang; Zeng,Jian; Zhou,Yong-Hong. |
| The phylogenetic relationships of 15 taxa from Hystrix and the related genera Leymus (NsXm), Elymus (StH), Pseudoroegneria (St), Hordeum (H), Psathyrostachys (Ns), and Thinopyrum (E) were examined by using the Giemsa C-banded karyotype. The Hy. patula C-banding pattern was similar to those of Elymus species, whereas C-banding patterns of the other Hystrix species were similar to those of Leymus species. The results suggest high genetic diversity within Hystrix, and support treating Hy. patula as E. hystrix L., and transferring Hy. coreana, Hy. duthiei ssp. duthiei and Hy. duthiei ssp. longearistata to the genus Leymus. On comparing C-banding patterns of Elymus species with their diploid ancestors (Pseudoroegneria and Hordeum), there are indications that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C-banding; Elymus; Genome; Hystrix; Leymus; Triticeae. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300015 |
| |
|
| |
|
|
| Salazar,Claudia; Valdivia,Gonzalo; Ardiles,Álvaro O.; Ewer,John; Palacios,Adrian G.. |
| The use of transgenic models for the study of neurodegenerative diseases has made valuable contributions to the field. However, some important limitations, including protein overexpression and general systemic compensation for the missing genes, has caused researchers to seek natural models that show the main biomarkers of neurodegenerative diseases during aging. Here we review some of these models-most of them rodents, focusing especially on the genetic variations in biomarkers for Alzheimer diseases, in order to explain their relationships with variants associated with the occurrence of the disease in humans. |
| Tipo: Journal article |
Palavras-chave: Degus; Genome; APP; APOE. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100014 |
| |
| Registros recuperados: 42 | |
|
|
|
