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Registros recuperados: 51
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A first generation integrated map of the rainbow trout genome Inra
Palti, Y.; Genet, C.; Luo, M.C.; Charlet, A.; Gao, G.; Hu, Y.; Castaño-Sánchez, C.; Tabet-Canale, K.; Krieg, F.; Yao, J.; Vallejo, R.L.; Rexroad III, C.E..
Background: Rainbow trout (Oncorhynchus mykiss) are the most-widely cultivated cold freshwater fish in the worldand an important model species for many research areas. Coupling great interest in this species as a researchmodel with the need for genetic improvement of aquaculture production efficiency traits justifies the continueddevelopment of genomics research resources. Many quantitative trait loci (QTL) have been identified forproduction and life-history traits in rainbow trout. An integrated physical and genetic map is needed to facilitatefine mapping of QTL and the selection of positional candidate genes for incorporation in marker-assisted selection(MAS) programs for improving rainbow trout aquaculture production.Results: The first generation...
Tipo: Journal Article Palavras-chave:  Rainbow trout (Oncorhynchus mykiss); Genomic research; QTL; Genome; Genetic maps.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201141cecd7b&uri=/notices/prodinra1/2011/06/
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A strategy to identify genomic expression at single-cell level or a small number of cells Electron. J. Biotechnol.
Li,Biaoru.
Recent advances in functional genomics allow us to estimate the expression of several thousands of genes in the mammalian genome. Techniques such as microarrays, expressed tag sequencing (EST), serial analysis of gene expression (SAGE), subtractive cloning and differential display (DD), and two-dimensional electrophoresis gel have been extensively used to screen and analyze parallel gene expression. Some pathological processes, for example, tumorigenesis and solid tumour growth, in which the former is derived from a single-cell and the latter has a mixed-cell problem, present new challenges to the limit of these functional genomic techniques. To fully understand the functions of cells in tumorigenesis or in heterogeneous solid tumour masses, it is...
Tipo: Journal article Palavras-chave: Genome; Genomic expression; Genomic expression analysis at single cell; Single cell.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100011
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Ant colony algorithm for analysis of gene interaction in high-dimensional association data R. Bras. Zootec.
Rekaya,Romdhane; Robbins,Kelly.
In recent years there has been much focus on the use of single nucleotide polymorphism (SNP) fine genome mapping to identify causative mutations for traits of interest; however, many studies focus only on the marginal effects of markers, ignoring potential gene interactions. Simulation studies have show that this approach may not be powerful enough to detect important loci when gene interactions are present. While several studies have examined potential gene interaction, they tend to focus on a small number of SNP markers. Given the prohibitive computation cost of modeling interactions in studies involving a large number SNP, methods need to be develop that can account for potential gene interactions in a computationally efficient manner. This study adopts...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genome; Simulation; SNP.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300011
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Araucaria angustifolia chloroplast genome sequence and its relation to other Araucariaceae Genet. Mol. Biol.
Brandão,José Henrique S. G.; Rodrigues,Nureyev F.; Eguiluz,Maria; Guzman,Frank; Margis,Rogerio.
Abstract Araucaria angustifolia is endemic to southern Brazil. Known as Brazilian pine, A. angustifolia is the only native conifer species with economic and social relevance in this country. Due to massive exploitation, it has suffered a significant population decline and currently is classified as critically endangered. This encouraged the scientific community to investigate genetic features in Brazilian pine to increase resources for management and preservation. In this work, RNA-Seq data was used to determine the complete nucleotide sequence of the A. angustifolia chloroplast genome (cpDNA). The cpDNA is 146,203 bp in length and contains 122 genes, including 80 protein-coding genes, 5 ribosomal RNA genes, and 37 tRNA genes. Coding regions comprise...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazilian pine; Plastid; Genome; CpDNA; Conservation.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400671
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Attitudes towards genomic: Research in four Latin American countries Electron. J. Biotechnol.
Rodríguez Yunta,Eduardo; Valdebenito Herrera,Carolina; Misseroni,Adelio; Fernández Milla,Lautaro; Outomuro,Delia; Schiattino Lemus,Irene; Ferrer Lues,Marcela; Lolas Stepke,Fernando.
The present reflection refers to data obtained about the social representations of genomic research and its applications through interviews with legislators and lawyers, biomedical researchers and civilians and the review of scientific and legal literature in four Latin American countries: Argentine, Chile, Mexico and Peru. Several issues are addressed: little access to prevention and therapeutic methods, lack of equity in health benefits, commercialization of gene sequences through patents which leads to commercial exploitation of underdeveloped countries, the possibility of physical or psychological damage or genetic discrimination, the possibility of genetic modifications or abortion for eugenic reasons, the necessity of safeguarding confidentiality,...
Tipo: Journal article Palavras-chave: Cloning; Genome; Social representations; Transgenic.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300003
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BACTERIAL DEGRADATION AND BIOREMEDIATION OF CHLORINATED HERBICIDES AND BIPHENYLS J. Soil Sci. Plant Nutr.
Seeger,Michael; Hernández,Marcela; Méndez,Valentina; Ponce,Bernardita; Córdova,Macarena; González,Myriam.
Chlorinated herbicides (e.g. s-triazines) and polychlorobiphenyls (PCBs) are persistent organic pollutants (POPs) that are widely distributed in the environment. s-Triazine herbicides are used in agriculture and forestry in diverse regions of the world. PCBs were produced worldwide for industrial applications, and an important amount of these compounds have been released into the environment. PCBs and s-triazines are toxic compounds that could act as endocrine disrupters and cause cancer. Therefore, environmental pollution with s-triazines and PCBs is of increasing concern. Bioremediation is an attractive technology for the decontamination of polluted sites. Microorganisms play a main role in the removal of POPs from the environment. Diverse bacteria able...
Tipo: Journal article Palavras-chave: Bacterial degradation; I-triazines; PCBs; Catabolic genes; Genome; Bioremediation.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162010000100007
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Comparative genome-wide polymorphic microsatellite markers in Antarctic penguins through next generation sequencing Genet. Mol. Biol.
Vianna,Juliana A.; Noll,Daly; Mura-Jornet,Isidora; Valenzuela-Guerra,Paulina; González-Acuña,Daniel; Navarro,Cristell; Loyola,David E.; Dantas,Gisele P. M..
Abstract Microsatellites are valuable molecular markers for evolutionary and ecological studies. Next generation sequencing is responsible for the increasing number of microsatellites for non-model species. Penguins of the Pygoscelis genus are comprised of three species: Adélie (P. adeliae), Chinstrap (P. antarcticus) and Gentoo penguin (P. papua), all distributed around Antarctica and the sub-Antarctic. The species have been affected differently by climate change, and the use of microsatellite markers will be crucial to monitor population dynamics. We characterized a large set of genome-wide microsatellites and evaluated polymorphisms in all three species. SOLiD reads were generated from the libraries of each species, identifying a large amount of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellite markers; Penguin; Genome; Polymorphism; Tetranucleotide.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400676
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Comparative genomics of closely related Thermococcus isolates, a genus of hyperthermophilic Archaea ArchiMer
Courtine, Damien.
The immense genomic diversity of microorganisms allows them to live everywhere, even in extreme environments such as deep hydrothermal vents. Scattered over the seabed, these are a good model for studying the biogeography and genomes diversification. A comparative genomics approach has been used on closely related isolates, of the genus Thermococcus. This work aimed at identifying mechanisms that have a role in the diversification of these genomes, and also to identify genes involved in this differentiation. For this purpose, two groups of about 20 isolates with different geographical origins were selected and sequenced.The geographical isolation resulting from colonization of new hydrothermal systems is likely to be a diversification and speciation factor...
Tipo: Text Palavras-chave: Génomique comparative; Thermococcus; Diversification; Génome; Hyperthermophile; Comparative!genomics; Thermococcus; Diversification; Genome; Hyperthermophile.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00609/72141/70893.pdf
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Complete genome sequence of acute viral necrosis virus associated with massive mortality outbreaks in the Chinese scallop, Chlamys farreri ArchiMer
Ren, Weicheng; Chen, Haixia; Renault, Tristan; Cai, Yuyong; Bai, Changming; Wang, Chongming; Huang, Jie.
Acute viral necrosis virus (AVNV) is the causative agent of a serious disease resulting in high mortality in cultured Chinese scallops, Chlamys farreri. We have sequenced and analyzed the complete genome of AVNV. The AVNV genome is a linear, double-stranded DNA molecule of 210,993 bp with a nucleotide composition of 38.5% G + C. A total of 123 open reading frames were predicted to encode functional proteins, ranging from 41 to 1,878 amino acid residues. The DNA sequence of AVNV is 97% identical to that of ostreid herpesvirus 1 (OsHV-1), and the amino acid sequences of the encoded proteins of these two viruses are 94-100% identical. The genomic organization of AVNV is similar to that of OsHV-1, and consists of two unique regions (170.4 kb and 3.4 kb,...
Tipo: Text Palavras-chave: Acute viral necrosis virus; Herpesvirus; OsHV-1; Genome; Scallop; Chlamys farreri; AVNV.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00135/24620/22650.pdf
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Computer simulations for biological aging and sexual reproduction Anais da ABC (AABC)
STAUFFER,DIETRICH; OLIVEIRA,PAULO M.C. DE; OLIVEIRA,SUZANA MOSS DE; PENNA,THADEU J.P.; MARTINS,JORGE S. SÁ.
The sexual version of the Penna model of biological aging, simulated since 1996, is compared here with alternative forms of reproduction as well as with models not involving aging. In particular we want to check how sexual forms of life could have evolved and won over earlier asexual forms hundreds of million years ago. This computer model is based on the mutation-accumulation theory of aging, using bits-strings to represent the genome. Its population dynamics is studied by Monte Carlo methods.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Parthenogenesis; Genome; Menopause; Testosterone; Monte Carlo simulation.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000100003
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Construction of a fosmid library of cucumber (Cucumis sativus) and comparative analyses of the eIF4E and eIF(iso)4E regions from cucumber and melon (Cucumis melo) National Institute of Agronomic Research
Havey, M.J.; Meyer, J.D.F.; Deleu, W.; Garcia-Mas, J..
We undertook comparative sequence analyses to assess synteny between the cucumber and melon genomes. A fosmid library of cucumber was synthesized and end sequencing of random fosmids produced over 680 kilobases, of which 25 % was similar to ribosomal DNAs, 23 % to satellite sequences, 22 % to coding regions in other plants, 4 % to transposable elements, 12 % to mitochondrial and chloroplast sequences, and 13 % showed no hits to the databases. The relatively high frequencies of ribosomal and satellite DNAs are consistent with previous analyses of cucumber DNA. Cucumber fosmids were selected and sequenced that carried eukaryotic initiation factors eIF4E and eIF(iso)4E, genes associated with recessively inherited resistances to potyviruses in plants. Indels...
Tipo: Conference Paper Palavras-chave: Eukaryotic initiation factors; Potyvirus resistance; Synteny; Genome; Sequence analyses; Orthologous genes; Genetic mapping.
Ano: 2008 URL: http://hdl.handle.net/2174/268
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Cucumber breeding and genomics: Potential from research with Cucumis hystrix National Institute of Agronomic Research
Jin-Feng Chen; Long-Zheng Chen; Yong Zhuang; You-Gen Chen; Xiao-Hui Zhou.
Cucumis hystrix Chakr. (2n=2x=24) is a wild Cucumis species found in Yunnan (China) having cross compatibility with cultivated cucumber (C. sativus L., 2n=2x=14). Since the first interspecific hybridization successfully made in 1995 and development of the synthetic allotetraploid species (C. X hytivus Chen and Kirkbride, 2n=4x=38) in 2000, experiments with C. hystrix and the progenies derived from this interspecific cross were carried out for the potentials in cucumber genetics and cultivar improvements. Some important characters or traits found in C. hystrix were introduced, and the novel germplasm developed from this cross and some results from genetic and epigenetic studies were summarized for the first time in this review. Future objectives are...
Tipo: Conference Paper Palavras-chave: Cucumis; Cucumber; Interspecific hybridization; Introgression; Wild species; Germplasm; Disease resistance; Photosynthesis; Genome; DNA methylation; Molecular markers.
Ano: 2008 URL: http://hdl.handle.net/2174/200
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Current genomic resources in melon and other cucurbits National Institute of Agronomic Research
Garcia-Mas, J..
In recent years there has been an effort towards the development of genomic resources in several cucurbit species, mainly in melon, cucumber and watermelon. The number of available sequences for these species in public databases has increased recently. This has allowed the design of microarrays for transcriptome analysis and the discovery of SNP markers for genotyping. Other genomic tools are also available in melon as BAC libraries, mutant populations and saturated genetic maps. Some international initiatives have emerged that will probably lead to the sequencing of the complete genomes of some cucurbit species during the next years.
Tipo: Conference Paper Palavras-chave: Genomic tool; Microarray; EST; Mutants; Physical map; SNP markers; BAC libraries; Genetic maps; EST databases; Genome; QTL.
Ano: 2008 URL: http://hdl.handle.net/2174/213
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Cytoplasmic and nuclear genome differentiation in A-genome diploid species of rice as revealed by the restriction fragment length polymorphism analysis of DNAs International Rice Research Institute
Ishii, Takeshige.
115 leaves : ill. Thesis (Ph.D.) -- Kyoto University
Tipo: Thesis Palavras-chave: Rice; Plant genetics; DNA; Genome; Nuclear genome; Diploidy; Restriction fragment length polymorphism.
Ano: 1991 URL: http://hdl.handle.net/123456789/947
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Detección e incidencia del fitoplasma Maize Bushy Stunt y su relación con el rendimiento en maíz en el estado de Veracruz, México Colegio de Postgraduados
Alcántara Mendoza, Susana.
Una nueva enfermedad del maíz apareció en el estado de Veracruz, México durante 2003-2004. Síntomas de amarillamiento, enrojecimiento y posterior quemado de hojas, proliferación de mazorcas, jilotes raquíticos, falta de fecundación y granos vanos fueron parte del síndrome de la enfermedad. El fitoplasma Maize Bushy Stunt (MBS) se identifico por PCR se detectó en plantas con los síntomas antes descritos, en los municipios de Tlalixcoyan, Cosoleacaque y Paso de Ovejas en 2006 y 2007. Los genotipos CP-562, 30F83, 30F92, Orca, 30F96, Nutria y Asgrow 7573 resultaron positivos a la presencia del MBS, mientras que en 3086, 30F97 y CP-560 no se detectó la presencia del MBS en una plantación experimental en plantas sintomáticas en Tlalixcoyan, Ver. en 2006 y 2007....
Palavras-chave: Maize Bushy Stunt; PCR; Incidencia; Resistencia genética; Genoma; Homología; Incidence; Genetic resistance; Genome; Homology; Maestría en Ciencias; Postgrado en Fitosanidad; Fitopatología.
Ano: 2009 URL: http://hdl.handle.net/10521/53
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Detección e incidencia del fitoplasma Maize Bushy Stunt y su relación con el rendimiento en maíz en el estado de Veracruz, México Colegio de Postgraduados
Alcántara Mendoza, Susana.
Una nueva enfermedad del maíz apareció en el estado de Veracruz, México durante 2003-2004. Síntomas de amarillamiento, enrojecimiento y posterior quemado de hojas, proliferación de mazorcas, jilotes raquíticos, falta de fecundación y granos vanos fueron parte del síndrome de la enfermedad. El fitoplasma Maize Bushy Stunt (MBS) se identifico por PCR se detectó en plantas con los síntomas antes descritos, en los municipios de Tlalixcoyan, Cosoleacaque y Paso de Ovejas en 2006 y 2007. Los genotipos CP-562, 30F83, 30F92, Orca, 30F96, Nutria y Asgrow 7573 resultaron positivos a la presencia del MBS, mientras que en 3086, 30F97 y CP-560 no se detectó la presencia del MBS en una plantación experimental en plantas sintomáticas en Tlalixcoyan, Ver. en 2006 y 2007....
Palavras-chave: Maize Bushy Stunt; PCR; Incidencia; Resistencia genética; Genoma; Homología; Incidence; Genetic resistance; Genome; Homology; Maestría; Postgrado en Fitosanidad; Fitopatología.
Ano: 2009 URL: http://hdl.handle.net/10521/53
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Draft genome sequence of Chryseobacterium limigenitum SUR2T (LMG 28734T) isolated from dehydrated sludge BJM
Škraban,Jure; Kyrpides,Nikos C.; Shapiro,Nicole; Whitman,William B.; Trček,Janja.
ABSTRACT The type strain SUR2 of the novel species Chryseobacterium limigenitum was isolated from a dehydrated sludge of the municipal sewage treatment plant in Dogoše near Maribor in Slovenia. The draft genome, with 60 contigs, 4,697,725 bp, 34.4% of G+C content, was obtained using the Illumina HiSeq 2500-1 platform. Joint Genome Institute Microbial Genome Annotation Pipeline (MGAP v.4) has identified 4322 protein-coding sequences including resistance genes against arsenic and other heavy metals. In addition, a subclass B3 metallo-β-lactamase, which confers resistance to penicillins, cephalosporins and carbapenems, was also present in the genome. The genome sequence provides important information regarding bioremediation potential and pathogenic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chryseobacterium limigenitum; Genome; Arsenite; Lactamase.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100005
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Draft genome sequence of pectic polysaccharide-degrading moderate thermophilic bacterium Geobacillus thermodenitrificans DSM 101594 BJM
Petkauskaite,Raimonda; Blom,Jochen; Goesmann,Alexander; Kuisiene,Nomeda.
Abstract Geobacillus thermodenitrificans DSM 101594 was isolated as a producer of extracellular thermostable pectic polysaccharide degrading enzymes. The completely sequenced genome was 3.6 Mb in length with GC content of 48.86%. A number of genes encoding enzymatic active against the high molecular weight polysaccharides of potential biotechnological importance were identified in the genome.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Geobacilli; Polysaccharide degradation; Genome.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100007
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Draft genomes and phenotypic characterization of Tisochrysis lutea strains. Toward the production of domesticated strains with high added value ArchiMer
Carrier, Gregory; Baroukh, Caroline; Rouxel, Catherine; Duboscq-bidot, Laetitia; Schreiber, Nathalie; Bougaran, Gael.
Tisochrysis lutea is a microalga species currently used in aquaculture as a feed for shellfish, oysters and shrimps. It also has many other potential industrial applications, such as the production of neutral lipids for biofuels or the production of ω-3 fatty acids for nutraceuticals (human food complements). To efficiently exploit the potential of this microalga, however, higher lipid productivities are needed. To this end, improvement programs need to be developed and optimized. The diversity of strains available in microalgae has not yet been exploited in such improvement programs. In this study, the intra-strain diversity was observed and exploited to increase neutral lipid productivity. New clonal strains with higher neutral lipid productivity were...
Tipo: Text Palavras-chave: Microalgae; Genome; Improvement program; Lipids; Tisochrysis lutea.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00411/52204/52927.pdf
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Expression profile of oxidative and antioxidative stress enzymes based on ESTs approach of citrus Genet. Mol. Biol.
Peroni,Luis Antonio; Ferreira,Renato Rodrigues; Figueira,Antonio; Machado,Marcos Antonio; Stach-Machado,Dagmar Ruth.
Plants not only evolve but also reduce oxygen in photosynthesis. An inevitable consequence of this normal process is the production of reactive oxygen species (ROS). Plants are adequately protected by the presence of multiple antioxidative enzymes in the cytosol and also in the different cell organelles such as chloroplasts, mitochondria, and peroxisomes. Traditionally, ROS were considered to be only a toxic byproduct of aerobic metabolism. However, recently it has become apparent that plants actively produce these molecules which may control many different physiological processes such as abiotic and biotic stress response, pathogen defense and systemic signaling. The search results using the Citrus Genome Program in Brazil (CitEST) for oxidative stress...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus; Genome; EST; Reactive oxygen species; Oxidative stress enzyme.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500016
Registros recuperados: 51
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