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A possible relationship between gluconeogenesis and glycogen metabolism in rabbits during myocardial ischemia Anais da ABC (AABC)
AGUIAR,RAQUEL R. DE; VALE,DANIELA F.; SILVA,RENATO M. DA; MUNIZ,YOLANDA P.; ANTUNES,FERNANDA; LOGULLO,CARLOS; OLIVEIRA,ANDRÉ L.A.; ALMEIDA,ADRIANA J. DE.
ABSTRACT Ischemia is responsible for many metabolic abnormalities in the heart, causing changes in organ function. One of modifications occurring in the ischemic cell is changing from aerobic to anaerobic metabolism. This change causes the predominance of the use of carbohydrates as an energy substrate instead of lipids. In this case, the glycogen is essential to the maintenance of heart energy intake, being an important reserve to resist the stress caused by hypoxia, using glycolysis and lactic acid fermentation. In order to study the glucose anaerobic pathways utilization and understand the metabolic adaptations, New Zealand white rabbits were subjected to ischemia caused by Inflow occlusion technique. The animals were monitored during surgery by pH and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene expression; Glycogen; Ischemia; Myocardium; Rabbit.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401683
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Acclimation of juvenile Mugil liza Valenciennes, 1836 (Mugiliformes: Mugilidae) to different environmental salinities Neotropical Ichthyology
Lisboa,Viviana; Barcarolli,Indianara F.; Sampaio,Luís A.; Bianchini,Adalto.
Survival and physiological parameters associated with metabolism and osmoregulation were evaluated in juveniles of the Lebranche mullet Mugil liza acclimated to different water salinities (5, 10, 20, 30, and 40‰) for 15 days. Room temperature (25ºC) and photoperiod (12L:12D) were fixed. Fish were fed twice-a-day with commercial diet (28% crude protein) until satiation. After acclimation, whole body oxygen consumption was measured and fish were euthanized and sampled for blood, gills, and liver. Whole body oxygen consumption and plasma osmolality did not change in the range of salinities tested. The isosmotic point was estimated as 412.7 mOsmol kg-1 (13.5‰). Gill Na+,K+-ATPase activity tended to be lower at 20 and 30‰, while liver glycogen content was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Glycogen; Na+; K+-ATPase; Osmoregulation; Oxygen consumption.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300591
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Cardiac rhabdomyoma in a slaughtered pig Ciência Rural
Pereira,Paula Reis; Cruz,Raquel Aparecida Sales da; Jühlich,Luciane Maria; Sonne,Luciana; Barcellos,David Emílio Santos Neves de; Driemeier,David.
ABSTRACT: Rhabdomyoma is a rare neoplasm of striated muscle that occurs predominantly in the myocardium. In animals, cardiac rhabdomyoma are observed as incidental lesions in slaughter pigs and have been rarely described in other species, such as cattle, dogs, and deer. This report describes a case of cardiac rhabdomyoma in a male pig at slaughter age that died suddenly in the pre-slaughter period. At necropsy, multiple nodules were observed in the right and left ventricular walls and the interventricular septum. Histopathological examination showed neoplastic proliferation composed of polyhedral cells, with vacuolated cytoplasm that sometimes presented a “spider cell” appearance. Histochemical staining with Schiff’s periodic acid revealed glycogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pig; Cardiac tumor; Glycogen; Spider cell; Immunohistochemistry.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000553
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Correlation between the Presence of Ubiquitin Conjugated Protein Phosphatase Inhibitor 1 and Postmortem Muscle Glycogen Metabolism OAK
NYAM-OSOR, Purevdorj; SHIMADA, Kenichiro; HAN, Kyu-Ho; FUKUSHIMA, Michihiro; SEKIKAWA, Mistuo; 島田, 謙一郎; 韓, 圭鎬; 福島, 道広; 関川, 三男.
Recently, we have identified the ubiquitin conjugate, protein phosphatase inhibitor 1 (I1), from postmortem muscles. Since I1 is one of the important regulators of glycogen metabolism, the effect of this conjugate (Ub-I1) present in postmortem muscle on glycolysis was investigated. Two sample groups were distinguishable: those not containing Ub-I1 (I, n=5)and those containing Ub-I1 (II, n=27). Due to the enormous variation in ultimate pH at 48 h postmortem, the latter group was subcategorized into a IIa group showing lower or normal pH and a IIb group showing higher or dark, firm, dry meat pH. Postmortem glycolysis occurred rapidly and vigorously among group I samples. The rate and extent of postmortem glycolysis was typical in group IIa samples while it...
Palavras-chave: Ubiquitin conjugate; Protein phosphatase inhibitor 1; Glycogen; Glycolysis.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2961
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Diversification de la production conchylicole : cultures de palourdes sur estran (Compte rendu n°4) ArchiMer
Goulletquer, Philippe.
Winter mortalities of the manila clam rl1d1 tapes philippinarum along the french atlantic coasts : biochemical and ecophysio-logical aspects. For some years, high mortalities on Manila clam's breeding occured at the end of Winter on Atlantic coasts. The ecophysioIogicaI survey of population was performed using a ground culture technique. This permitted to characterize the conditions related with mortalities such as a loss in dry weight and a decrease of metabol ic reserves. A comparison of biochemical composition was conducted during the occurence of mortality on different sites along the Atlantic coast, and in different ecosystems (tidal land, oysters ponds).
Tipo: Text Palavras-chave: Tidal bottom; Oyster pond; Glycogen; Ruditaoes phiIippinarum; Mortality; Estran; Claire ostréicole; Glycogène; Ruditaoes phiIippinarum; Mortalité.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/rapport-1566.pdf
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Effect of salinity on survival, growth and biochemical parameters in juvenile Lebranch mullet Mugil liza (Perciformes: Mugilidae) Neotropical Ichthyology
Lisboa,Viviana; Barcarolli,Indianara Fernanda; Sampaio,Luís André; Bianchini,Adalto.
Teleost fish growth may be improved under isosmotic condition. Growth and metabolic performance of juvenile Mugil liza (isosmotic point: 12‰) were evaluated after 40 days in different salinities (0, 6, 12 and 24‰). Tests were performed in quadruplicate (30 fish/tank; 0.48 ± 0.1 g body weight; 3.27 ± 0.1 cm total length) under controlled water temperature (28.2 ± 0.1ºC) and oxygen content (>90% saturation). Fish were fed on artificial diet (50% crude protein) four times a day until apparent satiation. Results showed that salinity influenced juvenile mullet growth. Fish reared at salinity 24‰ grew better than those maintained in freshwater (salinity 0‰). Gill Na+,K+-ATPase activity and whole body oxygen consumption showed an U-shape-type response over the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycogen; Growth; Metabolism; Na+; K+-ATPase.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000200447
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Glycogen Determination in Bovine Muscle: A Proposal for Rapid Determination Chilean J. Agric. Res.
Hargreaves B,Antonio; Barrales V,Luis; Barrales Z,Daniela; Riveros F,José Luis; Peña R,Iván.
A practical, rapid and inexpensive method for muscle glucose determination is proposed. The method is based on acid digestion of the sample, followed by neutralization of the solution and the subsequent glucose determination using a domestic glucometer for human blood glucose level control. In a pre-experimental phase, the accuracy of this methodology and the titration method was determined, comparing results to pre-established glucose concentrations. There were significant differences between the glucose reading given by the glucometer and the pre-established glucose concentrations and the results given by the titration method. Nevertheless, when discrepancies (bias) were identified, differences resulted non-significant (P = 0.85). Subsequently, muscle...
Tipo: Journal article Palavras-chave: Muscle; Glucose; Glycogen; Titration; Glycolytic potential.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300009
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Influence de la densite et de l'age des huitres japonaises (Crassostrea gigas ) sur la croissance et la composition biochimique en bassins de terre ArchiMer
Bodoy, A; Geiaron, P; Garnier, Jacqueline; Heurtebise, S.
Two different biomasses (100 g multiplied by m super(-2) and 1 kg multiplied by m super(-2)) of oysters were put in identical sea-water ponds, thus leading to different trophic conditions, corresponding to rearing techniques. Changes in growth, biochemical composition and sexual maturation were recorded for two age groups. The first one included 9 month-old oysters and the second one 21 month-old oysters, at the beginning of the experiment. For both age groups, significant differences were observed in growth and biochemical composition. Lipids were accumulated faster for the lower biomass. However, the only difference concerning glycogen was observed on the older oysters. They showed a peak at the lowest biomass and a decrease at the larger biomass. Sexual...
Tipo: Text Palavras-chave: Crassostrea gigas; Bivalvia; Sea water; Oyster culture; Glycogen; Age; Chemical composition; Lipids; Biochemistry; Sexual maturity; Rearing; Pond culture; Biomass; Growth; Density.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-916.pdf
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Intermittent sustained swimming in 'matrinxã' Brycon amazonicus (Bryconidae: Bryconinae): hematological and metabolic responses Neotropical Ichthyology
Fabrizzi,Fernando; Moraes,Gilberto; Hackbarth,Araceli; Almeida,Luciana Cristina de; Arbeláez-Rojas,Gustavo; Nunes,Cleujosí da Silva.
In fish, studies on a wide variety of physiological effects of exercise have been reported since a long time. It has been attributed special attention to some types of exercise, however, its application as a healthful practice in the rearing and welfare of farming fish is rising in last few years. In this particular, long-term intermittent sustained swimming (ISS) has been not yet explored. In this work, the freshwater fish Brycon amazonicus was submitted to (ISS) for 30 days at velocity of 1.0 body-length sec-1 for 12h interspaced by 12h under still water. Hematology and metabolism were evaluated. Exercised fish decreased 30% the erythrocyte number and hemoglobin was unvaried. The stores of liver glycogen and muscular triacylglycerol (TAG) were increased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adaptation; Exercise; Fish; Glycogen; Metabolism; Triacylglycerol.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000200425
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Lairage time effect on meat quality in Hereford steers in rangeland conditions R. Bras. Zootec.
Costa,Franciely de Oliveira; Brito,Gustavo; Lima,Juan Manuel Soares de; Sant’Anna,Aline Cristina; Costa,Mateus José Rodrigues Paranhos da; Campo,Marcia del.
ABSTRACT This study evaluated lairage time effects on carcass and meat quality traits of Hereford steers. Thirty Hereford steers fed on pasture were assigned to two treatments according to lairage time: 3 h (n = 15) and 12 h (n = 15). Individual temperament was assessed using crush score and flight speed. pH decline, glycogen content, meat color, and shear force were measured. pH was not different between treatments at any time point, showing a normal decline rate. Meat color and shear force did not differ between treatments, but muscle glycogen was lower in treatment with 3 h, not enough to affect quality but suggesting a higher level of stress in 3 h lairage time. Temperament did not have any impact on carcass and meat quality traits. No differences were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crush score; Flight speed; Glycogen; PH; Shear force.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100702
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Metabolic substrates are not mobilized from the osmoregulatory organs (gills and kidney) of the estuarine pufferfishes Sphoeroides greeleyi and S. testudineus upon short-term salinity reduction Neotropical Ichthyology
Prodocimo,Viviane; Souza,Carine F.; Pessini,Cristina; Fernandes,Luiz Claudio; Freire,Carolina A..
The marine-estuarine species of pufferfishes Sphoeroides testudineus and S. greeleyi are very efficient osmoregulators. However, they differ with respect to their tolerance of salinity reduction. During low tide S. testudineus remains in diluted estuarine waters, whereas S. greeleyi returns to seawater (SW). The hypothesis tested here was that the short-term mobilization of metabolic substrates stored in their main osmoregulatory organs would correlate with this differential tolerance. Fishes exposed to 5‰ (for 6 h) were compared to those kept in 35‰. Branchial and renal contents of triglycerides, protein and glycogen were evaluated, and total ATPase activity accounted for the tissues' metabolism. Plasma osmolality, chloride and glucose, hematocrit, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish gill; Fish kidney; Glycogen; Metabolism; Osmoregulation; Tetraodontidae.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000400009
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Mobilization of endogenous glycogen and trehalose of industrial yeasts BJM
Paulillo,Silene Cristina de Lima; Yokoya,Fumio; Basso,Luiz Carlos.
The fermentation of yeast reserve carbohydrates, glycogen and trehalose is a procedure to increase protein level of yeast cells and improve ethanol production. This work studied on the degradation kinetics of glycogen and trehalose carried out with two industrial strains of Saccharomyces cerevisiae (PE-2 and SA-1) and the effect of different temperatures (38º, 40º, 42º and 44ºC) on degradation rate. Endogenous fermentation was carried out in a yeast suspension at 20% (w/v) based on wet weight, suspended in fermented media with 3.0 to 4.5 % (v/v) of ethanol. The degradation of the carbohydrate reserves at 40ºC followed first-order kinetics, showing that its rate is mainly dependent on the carbohydrate concentration in the cell. The degradation rate (k)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; Glycogen; Trehalose; Yeast; Fermentation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300014
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Mobilization of energetic substrates in the endangered catfish Steindachneridion parahybae (Siluriformes: Pimelodidae): changes in annual reproductive cycle in captivity Neotropical Ichthyology
Tolussi,Carlos E.; Gomes,Aline D.; Ribeiro,Cristiéle da S.; Caneppele,Danilo; Moreira,Renata G.; Honji,Renato M..
ABSTRACT This study aimed at analyzing the energetic substrate (ES) in the main storage tissues of Steindachneridion parahybae, throughout the reproductive cycle in captivity. Differently from wild, in captivity, feeding is not interrupted during the reproductive period, the females do not spawn spontaneously, and they are sedentary. Adult females were sampled monthly and based on their histology and gonadosomatic index (GSI), ovaries were classified into: previtellogenic (PRV), vitellogenic (VTG), and regression (REG) stages. Ovaries at the VTG stage showed higher protein and lipids levels than at the PRV stage with a positive correlation between these substrates and the GSI. Muscle was the main source of proteins transferred to the ovaries, according to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycogen; Lipids; Metabolic substrate; Protein; Reproductive cycle.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000200208
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Molecular cloning and seasonal expression of oyster glycogen phosphorylase and glycogen synthase genes ArchiMer
Bacca, Helene; Huvet, Arnaud; Fabioux, Caroline; Daniel, Jean-yves; Delaporte, Maryse; Pouvreau, Stephane; Van Wormhoudt, A; Moal, Jeanne.
To investigate the control at the mRNA level of glycogen metabolism in the cupped oyster Crassostrea gigas, we report in the present paper the cloning and characterization of glycogen phosphorylase and synthase cDNAs (Cg-GPH and Cg-GYS, respectively, transcripts of main enzymes for glycogen use and storage), and their first expression profiles depending on oyster tissues and seasons. A strong expression of both genes was observed in the labial palps and the gonad in accordance with specific cells located in both tissues and ability to store glucose. Cg-GPH expression was also found mainly in muscle suggesting ability to use glycogen as readily available glucose to. supply its activity. For seasonal examinations, expression of Cg-GYS and Cg-GPH genes...
Tipo: Text Palavras-chave: Reproduction; Regulation; Oyster; Glycogen; Expression; Gene; Energy; Crassostrea gigas; Bivalve.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-435.pdf
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Mortalité hivernale chez la palourde japonaise Ruditapes philippinarum sur le littoral atlantique : aspects biochimique et écophysiologique ArchiMer
Goulletquer, Philippe.
For some years, high mortalities on Manila clam's bredding occured at the end of winter on Atlantic coasts. The ecophysiological survey of population was performed using a ground culture technique. This permitted to characterize the conditions related with mortalities such as a loss in dry weight and a decrease of metabolic reserves. A comparison of biochemical composition was conducted during the occurence of mortality on different sites along the Atlantic coast, and in different ecosystems (tidal land, oysters ponds).
Tipo: Text Palavras-chave: Tidal bottom; Oyster pond; Glycogen; Ruditapes philippinarum; Mortality; Estran; Claires ostréicoles; Glycogène; Ruditapes philippinarum; Mortalité.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/publication-3019.pdf
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Partial recovery of erythrocyte glycogen in diabetic rats treated with phenobarbital BJMBR
Erythrocytes may play a role in glucose homeostasis during the postprandial period. Erythrocytes from diabetic patients are defective in glucose transport and metabolism, functions that may affect glycogen storage. Phenobarbital, a hepatic enzyme inducer, has been used in the treatment of patients with non-insulin-dependent diabetes mellitus (NIDDM), increasing the insulin-mediated glucose disposal. We studied the effects of phenobarbital treatment in vivo on glycemia and erythrocyte glycogen content in control and alloxan-diabetic rats during the postprandial period. In control rats (blood glucose, 73 to 111 mg/dl in femoral and suprahepatic veins) the erythrocyte glycogen content was 45.4 ± 1.1 and 39.1 ± 0.8 µg/g Hb (mean ± SEM, N = 4-6) in the femoral...
Palavras-chave: Erythrocytes; Glycogen; Phenobarbital; Diabetes mellitus; Erythrocyte metabolism; Alloxan.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500014
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Physiological responses of female and male black-lip pearl oysters (Pinctada margaritifera) to different temperatures and concentrations of food ArchiMer
Chavez-villalba, Jorge; Soyez, Claude; Aurentz, Hermann; Le Moullac, Gilles.
This study was designed to measure responses of four-year-old black-lip pearl oysters (Pinctada margaritifera) to different temperatures and food concentrations and to identify the energy requirements of each sex. Oysters were fed a monospecific microalgal diet of Isochrysis affinis galbana (T-iso). Measurements of oxygen consumption and ingestion rates were carried out at 24 degrees C and 27 degrees C and at two algal (T-iso) concentrations: 5000 and 30 000 cell ml(-1). Glycogen content in adductor muscle, absorption efficiency and scope for growth were also estimated. Females and males responded differently to environmental factors, with food level being the most influential parameter. Oxygen consumption and absorption efficiency were significantly...
Tipo: Text Palavras-chave: Food supply; Ingestion rate; Glycogen; Oxygen consumption; Scope for growth; Pearl oyster.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26855/24980.pdf
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Physiological responses of pirarucu (Arapaima gigas) to acute handling stress Acta Amazonica
Gomes,Levy de Carvalho.
Pirarucu (Arapaima gigas) is an obligatory air-breathing fish from the Amazon basin. Previous study showed that pirarucu juveniles present a latency period in their response to moderate stress (transportation). Therefore the objective of this study was to verify the effects of a prolonged air exposure stress in lactate, glucose, cortisol, haematocrit, haemoglobin, and liver glycogen in pirarucu. Thirty-six fish were handled by netting and subjected to air exposure for 75-min. Six fish were sampled before handling and at 0, 6, 24, 48, and 96h after handling. Fish cortisol, lactate and haematocrit rose after handling, returning to previous unstressed values on the following sampling (6h after handling). Glucose increased significantly after handling and that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pirarucu; Stress; Air exposure; Glycogen; Cortisol; Glucose; Lactate; Haematocrit.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672007000400019
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Reproductive strategy of the invasive green mussel may result in increased competition with native fauna in the southeastern United States ArchiMer
Mcfarland, Katherine; Soudant, Philippe; Jean, Frederic; Volety, Aswani K..
Understanding the population dynamics of invasive species, such as the green mussel Perna viridis (Linnaeus, 1758), can aid in explaining the success of newly introduced populations and help predict the potential for spread. During a two-year field study of established populations in the invaded region of southwest Florida, year round gametogenesis and continuous spawning capabilities were observed through histological analysis of mussels collected monthly. This was supported by overall stable energetic reserves as measured through proximal biochemical composition (protein, glycogen and lipid content). However, egg outputs in the summer (6.4 x 10(6) +/- 2.6 x 10(6) eggs / female) were significantly higher than egg outputs of winter-spawned mussels (7.7 x...
Tipo: Text Palavras-chave: Gametogenesis; Protein; Glycogen; Lipid; Induced spawning.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00616/72842/72974.pdf
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Studies of Liver of Mice in the Course of an Experimental Infection with Babesia microti. I : Histopathological Changes and Changes in the Glycogen Content OAK
Kuzna-Grygiel, Wanda; Kolodziejczyk, Lidia.
Palavras-chave: Babesia microti; Liver; Histopathology; Glycogen.
Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/333
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