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Registros recuperados: 18
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Activation of Prophenoloxidase and Removal of Bacillus subtilis from the Hemolymph of Acheta domesticus (L.) (Orthoptera: Gryllidae) Neotropical Entomology
SILVA,CLEONOR C.A. DA.
Insect phenoloxidase is considered an important mediator in defense reaction against pathogens and parasites. It is present in the hemolymph of most insects as an inactive proenzyme, called prophenoloxidase. In Acheta domesticus (L.) the phenoloxidase activity was studied in the presence of Bacillus subtilis. The bacteria induced the activation of the prophenoloxidase and increased the level of phenoloxidase activity when injected into the hemocoel of A. domesticus nymphs. The level of increase was comparable to those observed in nymphs injected with laminarin, an activator of the proenzyme. Incubating B. subtilis in serum with phenoloxidase activated in vitro and then injecting into the insects accelerated the removal of the bacteria from the hemolymph in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemolynph; Phenoloxidase; Hemocyte; Defense reaction.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2002000300024
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Approche in vitro et in vivo des interactions hémocytes/parasite Bonamia ostreae, chez l'huître plate Ostrea edulis ArchiMer
Denoix, Michael.
Depuis la fin des années soixante, la conchyliculture française doit faire face à des mortalités importantes d'huître plate, Ostrea edulis, causées entre autres, par le protozoaire, Bonamia ostreae, responsable de la bonamiose. Peu de moyens sont à l'heure actuelle à la disposition des professionnels pour lutter contre cette maladie. L'obtention d'animaux résistants à cette infection est une voie retenue et explorée par l'Ifremer pour combattre la bonamiose et tenter d'apporter des solutions aux conchyliculteurs. Dans cette optique, l'Ifremer, a entrepris un programme de sélection pour obtenir des animaux résistants en exerçant une pression de sélection par inoculation de parasites purifiés dans la cavité péricardique d'animaux anesthésiés. Les premières...
Tipo: Text Palavras-chave: Huître plate; Ostrea edulis; Bonamia ostreae; Hemocyte; Parasite.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00033/14443/11742.pdf
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Combination of a pesticide exposure and a bacterial challenge: In vivo effects on immune response of Pacific oyster, Crassostrea gigas (Thunberg) ArchiMer
Gagnaire, Beatrice; Gay, Melanie; Huvet, Arnaud; Daniel, Jean-yves; Saulnier, Denis; Renault, Tristan.
To assess the impact of pollution induced by pesticides on Pacific oyster, Crassostrea gigas, health in France, in vivo effects of combined pesticide exposure and bacterial challenge on cell activities and gene expression in hemocytes were tested using flow cytometry and real-time PCR. As a first step, an in vivo model of experimental contamination was developed. Pacific oysters were exposed to a mixture of eight pesticides (atrazine, glyphosate, alachlor, metolachlor, fosetyl-alumimium, terbuthylazine, diuron and carbaryl) at environmentally relevant concentrations over a 7-day period. Hemocyte parameters (cell mortality, enzyme activities and phagocytosis) were monitored using flow cytometry and gene expression was evaluated by real-time PCR (RT-PCR)....
Tipo: Text Palavras-chave: Gene expression; Flow cytometry; Vibrio splendidus; Pathogenesis; Phagocytosis; Hemocyte; Pesticides; Bivalve immunity; Crassostrea gigas; Pacific oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3022.pdf
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Differential distribution and defence involvement of antimicrobial peptides in mussel ArchiMer
Mitta, Guillaume; Vandenbulcke, Franck; Noel, Thierry; Romestand, Bernard; Beauvillain, Jean Claude; Salzet, Michel; Roch, Philippe.
In previous papers, we characterised 3 types of 4-kDa, cysteine-rich, cationic antimicrobial peptides: MGDs (for Mytilus galloprovincialis defensins), mytilins and myticins, which are abundant in the mussel hemocytes. In the present work, me revealed a differential distribution of the cells expressing the different genes. In addition, using confocal and electron microscopy, we confirmed that defensins and mytilins were partially located in different sub-types of circulating hemocytes although the peptides can be located in the same cell, and even in the same granule. We also demonstrated that mytilins exert their microbicidal effect within the cells through the process of phagosome-mytilin granule fusion leading to the co-location of ingested bacteria and...
Tipo: Text Palavras-chave: Innate immunity; Hemocyte; Mussel; Myticin; Mytilin; Defensin; Antimicrobial.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-413.pdf
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Effect of a mono-specific algal diet on immune functions in two bivalve species - Crassostrea gigas and Ruditapes philippinarum ArchiMer
Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Lambert, Christophe; Quere, Claudie; Miner, Philippe; Choquet, Gwenaelle; Paillard, Christine; Samain, Jean-francois.
The impact of diets upon the fatty acid composition of haemocyte polar lipids and consequently upon immune parameters has been tested in the oyster Crassostrea gigas and the clam Ruditapes philippinarum. Oysters and clams were fed each of three cultured algae: Chaetoceros calcitrans, which is rich in 20:5(n-3) and 20:4(n-6) and poor in 22:6(n-3) fatty acids; T-Iso (Isochrysis sp.), which is rich in 22:6(n-3) and deficient in 20:5(n-3) and 20:4(n-6); and Tetraselmis suecica, which is deficient in 22:6(n-3) and contains only small amounts of 20:5(n-3) and 20:4(n-6). Fatty acid composition of haemocyte polar lipids was greatly affected by the diet. Oysters and clams fed C. calcitrans maintained a higher proportion of 20:5(n-3) and 20:4(n-6) in their haemocyte...
Tipo: Text Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Polyunsaturated fatty acids; Phagocytosis; Oxidative activity; Haemocyte membrane; Nutrition; Immunology; Bivalve; Immunologie; Bivalve; Ruditapes philippinarum; Crassostrea gigas; Acides gras poly insatures; Phagocytose; Flambee respiratoire; Membrane; Hemocyte; Nutrition.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-354.pdf
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Estudo comparativo do aspecto da hemolinfa de algumas espécies de carrapatos (Acari, Ixodidae): descrição da variação hemocitária de adultos de Amblyomma cajennense (Fabricius) Koch Zoologia
Carneiro,Maria Elisa; Daemon,Erik.
The hemocyte composition of adults of Amblyomma cajennense (Fabricius, 1787) Koch, 1844 adults consisted of three basic cellular types: prahemocyte I (PR I), plasmatocyte (PL) and spherulocyte (ES II). Among the ixodids observed (A. cajennense; A. dissimile Koch, 1844; Rhipicephalus sanguineus Latreille, 1806; Anocentor nitens (Neumann, 1897) Schulze, 1937; Boophilus microplus Canestrini, 1887 and Haemaphysalis sp.) only A. cajennense was different in the adult hemolymph colour. It changed from amber to deep blue as the ixodid age advanced. This fact could be associated with the presence of hemocyanin in the hemolymph and points out the need to develop further investigations about the Ixodidae hemolymph pratein composition as well as the function of all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ixodidae; Amblyomma; Ixodid; Hemolymph; Hemocyte.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752002000500011
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Étude des interactions entre infection à ostreid herpesvirus 1, immunité, autophagie et pesticides chez l’Huître creuse, Crassostrea gigas ArchiMer
Moreau, Pierrick.
The thesis work is part of the very current issue on mass mortality outbreaks affecting Pacific oyster, C. gigas, spat and juveniles and questions about the involvement of pesticides in this phenomenon. The first part of this thesis was devoted to study the effects of pesticides on hemocyte parameters in the Pacific oyster (in vitro and in vivo). The immunomodulator effect of selected pesticides (alone or in mixture) has been explored principally through flow cytometry. The second part concerned the study of the effects of a pesticides mixture on OsHV-1 itself. No direct effects have been reported on the viral particles in presence of the pollutants. Moreover, pesticide effects on Pacific oysters were also explored through experimental pathology assays...
Tipo: Text Palavras-chave: Pacific oyster; Pesticides; OsHV-1; Autophagy; Immunity; Hemocyte; Pathology.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00226/33740/32149.pdf
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Etude d'une prolifération hémocytaire chez la moule Mytilus edulis ArchiMer
Noel, Danièle.
Hemic proliferative disease in the bay mussel, Mytilus edulis, has been studied following mortalities in Washington State (USA). The disease has been reproduced in the labortory. The establishment of this experimental model led to study tumoral characteristics of the atypic hemocytes : ultrastrure, in vitro cultivation, transplantation. The infectious etiology of this tumor has been verified. It has been hypothesized to result from a viral infection since retrovirus-like particules have been observed following induction by bromodeoxyuridine. Specific monoclonal antibodies against atypic hemocytes have been obtained and characcterized
Tipo: Text Palavras-chave: Mytilus; Mollusque; Hémocyte; Tumeur; Ulstrastructure; In vitro culture; Transplantation; Rétrovirus; Anticorps monoclonaux; Mytilus; Mollusca; Hemocyte; Tumor; Ultrastructure; In vitro cultivation; Transplantation; Retrovirus; Monoclonal antibodies.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00428/53930/55057.pdf
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Evidence in oyster of a plasma extracellular superoxide dismutase which binds LPS ArchiMer
Gonzalez, Marcelo; Romestand, Bernard; Fievet, Julie; Huvet, Arnaud; Lebart, M; Gueguen, Yannick; Bachere, Evelyne.
We have characterized in the oyster Crassostrea gigas an extracellular superoxide dismutase (Cg-EcSOD) which appears to bind lipopolysaccharides (LPS). The protein has been purified from the oyster plasma and identified as a Cu/ZnSOD according to its N-terminal sequencing and biological activity. Cg-EcSOD expression and synthesis are restricted to hemocytes as revealed by in situ hybridization and immunocytochemistry. Cg-EcSOD-expressing hemocytes were seen in blood circulation, in connective tissues, and closely associated to endothelium blood vessels. Cg-EcSOD presents in its amino acid sequence a LPS-binding motif found in the endotoxin receptor CD14 and we show that the protein displays an affinity to Escherichia coli bacteria and with LPS and Lipid A....
Tipo: Text Palavras-chave: Beta integrin; Oxidative burst; Hemocyte; Crassostrea gigas; Invertebrate; Mollusc bivalve.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-3583.pdf
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Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI ArchiMer
Gonzalez, Marcelo; Gueguen, Yannick; Destoumieux Garzon, Delphine; Romestand, Bernard; Fievet, Julie; Pugniere, M; Roquet, F; Escoubas, Jean-michel; Vandenbulcke, F; Levy, O; Saune, Laure; Bulet, P; Bachere, Evelyne.
A cDNA sequence with homologies to members of the LPS-binding protein and bactericidal/permeability-increasing protein (BPI) family was identified in the oyster Crassostrea gigas. The recombinant protein was found to bind LIPS, to display bactericidal activity against Escherichia coli, and to increase the permeability of the bacterial cytoplasmic membrane. This indicated that it is a BPI rather than an LPS-binding protein. By in situ hybridization, the expression of the C gigas BPI (Cg-bpi) was found to be induced in hemocytes after oyster bacterial challenge and to be constitutive in various epithelia of unchallenged oysters. Thus, Cg-bpi transcripts were detected in the epithelial cells of tissues/organs in contact with the external environment (mantle,...
Tipo: Text Palavras-chave: Oyster innate immunity; Mollusk; Hemocyte; Epithelia; Antimicrobial.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3564.pdf
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Exposure to the toxic dinoflagellate Alexandrium catenella modulates juvenile oyster Crassostrea gigas hemocyte variables subjected to different biotic conditions ArchiMer
Lassudrie, Malwenn; Soudant, Philippe; Nicolas, Jean-louis; Miner, Philippe; Le Grand, Jacqueline; Lambert, Christophe; Le Goic, Nelly; Hegaret, Helene; Fabioux, Caroline.
The Pacific oyster Crassostrea gigas is an important commercial species cultured throughout the world. Oyster production practices often include transfers of animals into new environments that can be stressful, especially at young ages. This study was undertaken to determine if a toxic Alexandrium bloom, occurring repeatedly in French oyster beds, could modulate juvenile oyster cellular immune responses (i.e. hemocyte variables). We simulated planting on commercial beds by conducting a cohabitation exposure of juvenile, “specific pathogen-free” (SPF) oysters (naïve from the environment) with previously field-exposed oysters to induce interactions with new microorganisms. Indeed, toxic Alexandrium spp. exposures have been reported to modulate bivalve...
Tipo: Text Palavras-chave: Crassostrea gigas; Alexandrium; Harmful algal blooms; Hemocyte; Microbial challenge.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00314/42523/41894.pdf
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Hemócitos fagocitários em larvas de Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae) Zoologia
Falleiros,Angela M.F; Gregório,Elisa A.
The ultrastructural observation of the hemocytes of D. saccharalis larvae showed different hemocyte tvpes in various stage of interaction with microorganisms, which were interpreted as bacteria. The microorganisms were observed either free in the hemolymph or into the insect hemocytes. The bacteria were detected mostly into lhe granulocytes and plasmatocytes: the spherule cells and the oenocytoids also presented intracytoplasmic microorganisms, but less frequently. The fagocytic capacity of the different hemocyte tvpes is discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lepidoptera; Pyralidae; Diairaea saccharalis; Hemocyte; Phagocytosis; Ultrastructure.
Ano: 1995 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751995000400004
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Hemocyte characteristics in families of oysters, Crassostrea gigas, selected for differential survival during summer and reared in three sites ArchiMer
Lambert, Christophe; Soudant, Phillippe; Degremont, Lionel; Delaporte, Maryse; Moal, Jeanne; Boudry, Pierre; Jean, Frédéric; Huvet, Arnaud; Samain, Jean-francois.
High variability among individuals is often encountered when hemocyte characteristics are measured in bivalves. Such variability is suspected to result partly from genetic factors. In this study, hemocyte characteristics of six families of Crassostrea gigas were compared by flow cytometry at one sampling date in October 2001. These families were obtained from a nested, half-sibling cross design, and reared from July to October 2001 at three sites distributed along the French Atlantic coast from north to south: Baie des Veys (Normandy), Riviere d'Auray (Brittany) and Ronce (Marennes-oleron Basin, Poitou Charentes). Among the 15 measured hemocyte characteristics, production of reactive oxygen species (ROS) of untreated hemocytes (maintained in filtered...
Tipo: Text Palavras-chave: Genetic; Flow cytometry; Reactive oxygen species ROS; Adhesion; Hemocyte; Crassostrea gigas; Summer mortality.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3054.pdf
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Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone ArchiMer
Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine.
Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population...
Tipo: Text Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf
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Impact of diuron on aneuploidy and hemocyte parameters in Pacific oyster, Crassostrea gigas ArchiMer
Bouilly, Karine; Bonnard, Marc; Gagnaire, Beatrice; Renault, Tristan; Lapegue, Sylvie.
Diuron is a substituted urea herbicide used for agricultural and nonagricultural weed control. Its widespread use and relatively slow breakdown led us to analyze its influence on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in Pacific oysters, Crassostrea gigas. Adult oysters were subjected to two diuron concentrations (300 ng L-1 and 3 mu g L-1) for 11 weeks. Significantly higher aneuploidy level was observed in diuron-treated oysters compared with the control. Furthermore, the observed impact on aneuploidy persisted to the next generation as offspring exhibited significantly higher aneuploidy levels when their parents had been exposed to diuron. Significant increases in hemocyte parameters (cell...
Tipo: Text Palavras-chave: Diuron; Herbicide impact; Cytometry; Hemocyte; Aneuploidy; Crassostrea gigas.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2178.pdf
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Infection dynamics of a V. splendidus strain pathogenic to Mytilus edulis : In vivo and in vitro interactions with hemocytes ArchiMer
Ben Cheikh, Yosra; Travers, Marie-agnes; Le Foll, Frank.
The pathogenic strain V. splendidus 10/068 1T1 has previously been reported for its virulence to the blue mussel and for its capacity to alter immune responses. In this study, we expanded the knowledge on hemocyte-pathogen interactions by using in vitro and in vivo assays. V. splendidus 10/068 1T1 severely inhibited cell adhesion and acidic vacuole formation unlike the innocuous phylogenetically related V. splendidus 12/056 M24T1 which had no effect on these cell functions. Furthermore, the virulent bacteria decreased hemocyte viability (59% of viability after 24 h). Infection dynamics were explored by using a model based on water tank cohabitation with septic mussels infected by GFP-tagged V. splendidus 10/068 1T1. Experimental infections were...
Tipo: Text Palavras-chave: Innate immunity; Bivalves; Vibrio; Infection; Experimental model; Hemocyte.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00400/51136/53772.pdf
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The new insights into the oyster antimicrobial defense: Cellular, molecular and genetic view ArchiMer
Bachere, Evelyne; Rosa, Rafael Diego; Schmitt, Paulina; Poirier, Aurore; Merou, Nicolas; Charriere, Guillaume; Destoumieux-garzon, Delphine.
Oysters are sessile filter feeders that live in close association with abundant and diverse communities of microorganisms that form the oyster microbiota. In such an association, cellular and molecular mechanisms have evolved to maintain oyster homeostasis upon stressful conditions including infection and changing environments. We give here cellular and molecular insights into the Crassostrea gigas antimicrobial defense system with focus on antimicrobial peptides and proteins (AMPs). This review highlights the central role of the hemocytes in the modulation and control of oyster antimicrobial response. As vehicles for AMPs and other antimicrobial effectors, including reactive oxygen species (ROS), and together with epithelia, hemocytes provide the oyster...
Tipo: Text Palavras-chave: Mollusk; Immunity; Antimicrobial peptide; Hemocyte; Defensin.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00254/36552/35097.pdf
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Toxicity to bivalve hemocytes of pathogenic Vibrio cytoplasmic extract ArchiMer
Lambert, Christian; Nicolas, Jean-louis; Bultel, Valérie.
Using a chemiluminescence (CL) test, it had been previously demonstrated that Vibrio pectenicida, which is pathogenic to Pecten maximus larvae, was able to inhibit completely the CL activity of P, maximus hemocytes and partially inhibit those of Crassostrea gigas, Conversely, a Vibrio sp, strain, S322, pathogenic to C,gigas larvae was more active in reducing the CL activity of oyster hemocytes than of scallop hemocytes, Using this same CL biotest, V, pectenicida and S322 cytoplasmic extracts were shown to reproduce CL inhibition while the cytoplasmic extract of a nonpathogenic strain (U1, Pseudoalteromonas) was without effect. Moreover, cytoplasmic extract as well as live V, pectenicida cells provoked, within a few hours, death of P. maximus hemocytes...
Tipo: Text Palavras-chave: Bivalves; Hemocyte; Toxin; Chemiluminescence; Crassostrea gigas; Pecten maximus; Vibrio pectenicida.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-466.pdf
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