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Registros recuperados: 14
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Clinical biochemistry of rescued Magellanic Penguins (Spheniscus magellanicus): serum or heparinized plasma? Arq. Bras. Med. Vet. Zootec.
Morais,J.; Volpato,J.; Costa,A.; Kolesnikovas,C.; Souza,M.; Saito,M..
ABSTRACT In the last few years, an increasing number of debilitated Magellanic penguins (Spheniscus magellanicus) has been rescued and taken to rehabilitation centers on Brazil’s southern coast to be clinically treated and evaluated for re-introduction. This work aims to compare the viability of heparinized plasma with the viability of serum for biochemistry analyses under rehabilitation conditions. Blood sampled from 31 physically healthy rescued penguins was processed into serum/plasma-paired samples and analyzed for 12 biochemical parameters: alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), cholesterol (CHOL), creatine kinase (CK), gamma-glutamyl transpeptidase (GGT), glucose, (GLU) lactate dehydrogenase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Penguin; Rehabilitation; Blood sampling; Heparin; Optimization of resources.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300782
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Development of new heparin-like compounds and other antithrombotic drugs and their interaction with vascular endothelial cells BJMBR
Nader,H.B.; Pinhal,M.A.S.; Baú,E.C.; Castro,R.A.B.; Medeiros,G.F.; Chavante,S.F.; Leite,E.L.; Trindade,E.S.; Shinjo,S.K.; Rocha,H.A.O.; Tersariol,I.L.S.; Mendes,A.; Dietrich,C.P..
The anticlotting and antithrombotic activities of heparin, heparan sulfate, low molecular weight heparins, heparin and heparin-like compounds from various sources used in clinical practice or under development are briefly reviewed. Heparin isolated from shrimp mimics the pharmacological activities of low molecular weight heparins. A heparan sulfate from Artemia franciscana and a dermatan sulfate from tuna fish show a potent heparin cofactor II activity. A heparan sulfate derived from bovine pancreas has a potent antithrombotic activity in an arterial and venous thrombosis model with a negligible activity upon the serine proteases of the coagulation cascade. It is suggested that the antithrombotic activity of heparin and other antithrombotic agents is due...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heparin; Anticlotting activity heparan sulfate; Antithrombotic activity low molecular weight heparins; Preparation and antithrombotic activity antithrombotic agents; Development for clinical use crustacean heparin-like compounds; Antithrombotic activity antithrombotic agents vascular endothelial cells.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600002
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Effect of heparin on in vitro capacitation of boar sperm Biol. Res.
DAPINO,DORA G; MARINI,PATRICIA E; CABADA,MARCELO O.
Chlortetracycline (CTC) fluorescent pattern, the ability to undergo acrosome reaction (AR) upon exposure to 10 µM calcium ionophore A23187 and vitality estimation were used to investigate the effect of the sulfated glycosaminoglycan heparin on the in vitro capacitation of porcine spermatozoa. Sperm incubation in capacitating medium (CM) supplemented with 10 mM heparin for up to 120 min, showed an increase in the number of capacitated sperm (B pattern) and acrosome reacted sperm (AR pattern), without affecting their viability. In this condition, spermatozoa were incubated in CM depleted of albumin, calcium, bicarbonate or combinations, in the presence of heparin. In either calcium or bicarbonate-free media, capacitation was only basal and did not show...
Tipo: Journal article Palavras-chave: Boar sperm; Capacitation; Heparin; Sulfated glycosaminoglycans.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500006
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Effect of Low Molecular Weight Fucoidan and Low Molecular Weight Heparin in a Rabbit Model of Arterial Thrombosis ArchiMer
Durand, E; Helley, D; Zen, A; Dujols, C; Bruneval, P; Colliec-jouault, Sylvia; Fischer, Aurelie; Lafont, A.
Background: Therapeutic use of unfractionated heparin and low molecular weight heparins (LMWHs) is limited by hemorrhagic adverse effects. We compared the antithrombotic effect of LMW fucoidan (LMWF) and LMWH in an experimental model. Methods: Thrombosis was induced in femoral arteries of male New Zealand White rabbits by in situ induction of endothelial apoptosis with staurosporine (10(-5) M for 30 min). Starting the day before apoptosis induction, the animals received subcutaneous LMWF (15 mg/kg), LMWH (enoxaparin 2.5 mg/kg) or saline solution (control group) twice a day for 4 days. Results: The degrees of apoptosis and endothelial denudation were similar in the 3 groups. The thrombotic score was significantly lower in the LMWF group than in the LMWH and...
Tipo: Text Palavras-chave: Tissue factor; Tissue factor pathway inhibitor; Heparin; Fucoidan; Thrombosis.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4744.pdf
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Effects of a sulfated exopolysaccharide produced by Altermonas infernus on bone biology ArchiMer
Velasco, C. Ruiz; Baud'Huin, M.; Sinquin, Corinne; Maillasson, M.; Heymann, D.; Colliec-jouault, Sylvia; Padrines, M..
The growth and differentiation of bone cells is controlled by various factors which can be modulated by heparan sulphates. Here, we investigated the effects of an oversulphated exopolysaccharide (OS-EPS) on bone. We compared the effect of this compound with that of a native exopolysaccharide (EPS). Long-term administration of OS-EPS causes cancellous bone loss in mice due, in part, to an increase in the number of osteoclasts lining the trabecular bone surface. No significant difference in cancellous bone volume was found between EPS-treated mice and age-matched control mice, underlying the importance of sulphation in trabecular bone loss. However, the mechanism sustaining this osteoporosis was unclear. To clarify OS-EPS activities, we investigated the...
Tipo: Text Palavras-chave: Bone metabolism; Bone remodeling; Exopolysaccharide; Glycosaminoglycan; Heparin.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00032/14300/15749.pdf
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Heparin and commercial bothropic antivenom against the paralyzing effect of Bothrops jararacussu snake venom J. Venom. Anim. Toxins incl. Trop. Dis.
Rostelato-Ferreira,S; Rodrigues-Simioni,L; Oshima-Franco,Y.
The crude venom of Bothrops jararacussu (Bjssu) is known to induce muscular paralysis in vitro. Many studies have shown that various substances, including heparin, neutralize the damage caused by snake venom. In the present study, the ability of heparin (Hep) and commercial bothropic antivenom (CBA) to neutralize neuromuscular effects of Bjssu venom, at different time-points, was analyzed. Mouse phrenic nerve-diaphragm preparation was used through a conventional myographic technique, following five different protocols: Group 1 was incubated with Bjssu (40 µg/mL) without any other treatment; Groups 2 and 3 were pretreated with heparin (1 µL/mL) and CBA (120 µL/mL), respectively, for 15 minutes before venom addition; Group 4 after 50% neuromuscular blockade...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bothrops jararacussu; Heparin; Neutralization; Nerve-muscle preparation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100005
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In vivo antithrombotic properties of a heparin from the oocyte test cells of the sea squirt Styela plicata(Chordata-Tunicata) BJMBR
Cardilo-Reis,L.; Cavalcante,M.C.M.; Silveira,C.B.M.; Pavão,M.S.G..
In the ascidian Styela plicata, the oocytes are surrounded by two types of accessory cells named follicle cells and test cells. A heparin-like substance with an anticoagulant activity equivalent to 10% of mammalian heparin and about 5% as potent as the mammalian counterpart for the inhibition of thrombin by antithrombin was isolated from the oocyte test cells. In the present study, we compared the antithrombotic and hemorrhagic effects of sea squirt oocyte test cell heparin with those of porcine heparin in rat models of venous thrombosis and blood loss. Intravenous administration of the oocyte test cell heparin to Wistar rats (both sexes, weighing ~300 g, N = 4 in each group) at a dose of 5.0 mg/kg body weight, which produced a 1.8-fold increase in plasma...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascidian; Heparin; Experimental thrombosis; Antithrombotics; Hemorrhage.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100004
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Mini-Percoll processing of domestic ruminant frozen-thawed semen dispenses the use of heparin in capacitating medium. Repositório Alice
OLIVEIRA, C. C. S.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; ALFRADIQUE, V. A. P.; CÔRTES, L. R.; BRANDÃO, F. Z..
Sperm capacitation is a prerequisite for mammal successful fertilization. Although usually a capacitating substance such as heparin is used during sheep in vitro fertilization, evidences suggest that the cryopreservation process and Percoll technique could induce spontaneous capacitation. This study aimed to compare ovine, caprine and bovine frozen-thawed sperm after mini-Percoll processing on sperm parameters, receiving or not heparin supplementation. In conclusion, frozen-thawed ovine, caprine and bovine spermatozoa processed with mini-Percoll behave similarly regarding to capacitation status and does not require heparin supplementation during in vitro incubation to achieve capacitation. [Processamento pela técnica de mini-Percoll em sêmen...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sperm capacitation; Capacitação espermática; Ovino; Caprino; Bovino; Reprodução animal; Sêmen; Heparina; Sheep; Goats; Cattle; Spermatozoa; Reproduction; In vitro fertilization; Heparin.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080820
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Mini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin. Repositório Alice
ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z..
Background: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Billygoats; CTC; Caprino; Ovino; Bovino; Bode; Carneiro; Reprodução Animal; Sêmen; Heparina; Espermatozóide; Sheep; Goats; Cattle; Rams; Bulls; Animal reproduction; Sperm capacitation; Assisted reproductive technologies; Heparin.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1119218
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Proacrosin-acrosin activity in capacitated and acrosome reacted sperm from cryopreserved bovine semen Biocell
Rosatti,M. I.; Beconi,M. T.; Córdoba,M..
Acrosin activity is associated with normal fertility in human and bovine spermatozoa. The aim of the study was to determine the variation of the enzyme activity in the proacrosin-acrosin system in capacitated and acrosome reacted cryopreserved bovine sperm. Enzyme activity was assessed spectrophotometrically using N-a-benzoyl-DL-arginine p-nitroanilide (BAPNA) as specific substrate for acrosin at pH 8. Capacitation with heparin and quercitin failed to induce conversion of proacrosin to acrosin. An increase in acrosin activity produced by the presence of progesterone, in a dose-dependent manner, was related with the induction of true acrosome reaction. The total level of acrosin activity registered showed that 96% of acrosin of capacitated sperm samples and...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Bovine sperm; Proacrosin-acrosin; Heparin; Quercitin; Progesterone.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300007
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P-selectin, carcinoma metastasis and heparin: novel mechanistic connections with therapeutic implications BJMBR
Varki,A.; Varki,N.M..
Metastasis is a multistep cascade initiated when malignant cells penetrate the tissue surrounding the primary tumor and enter the bloodstream. Classic studies indicated that blood platelets form complexes around tumor cells in the circulation and facilitate metastases. In other work, the anticoagulant drug heparin diminished metastasis in murine models, as well is in preliminary human studies. However, attempts to follow up the latter observation using vitamin K antagonists failed, indicating that the primary mechanism of heparin action was unrelated to its anticoagulant properties. Other studies showed that the overexpression of sialylated fucosylated glycans in human carcinomas is associated with a poor prognosis. We have now brought all these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heparin; Carcinomas; P-selectin; Mucin; Metastasis.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600003
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Structure and anticoagulant properties of sulfated glycosaminoglycans from primitive Chordates Anais da ABC (AABC)
PAVÃO,MAURO S. G..
Dermatan sulfates and heparin, similar to the mammalian glycosaminoglycans, but with differences in the degree and position of sulfation were previously isolated from the body of the ascidian Styela plicata and Ascidia nigra. These differences produce profound effects on their anticoagulant properties. S. plicata dermatan sulfate composed by 2-O-sulfatedalpha-L-iduronic acid and 4-O-sulfated N-acetyl-beta-D-galactosamine residues is a potent anticoagulant due to a high heparin cofactor II activity. Surprisingly, it has a lower potency to prevent thrombus formation on an experimental model and a lower bleeding effect in rats than the mammalian dermatan sulfate. In contrast, A. nigra dermatan sulfate, also enriched in 2-O-sulfated alpha-L-iduronic acid, but...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascidian; Heparin; Dermatan sulfate; Heparin cofactor II; Anticoagulant; Antithrombotic.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000100007
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The functional links between prion protein and copper Biol. Res.
VARELA-NALLAR,LORENA; TOLEDO,ENRIQUE M; CHACÓN,MARCELO A; INESTROSA,NIBALDO C.
Prion diseases are fatal neurodegenerative disorders associated with the conversion of the cellular prion protein (PrPC) into a pathologic isoform. Although the physiological function of PrPC remains unknown, evidence relates PrPC to copper metabolism and oxidative stress as suggested by its copper-binding properties in the N-terminal octapeptide repeat region. This region also reduces copper ions in vitro, and this reduction ability is associated with the neuroprotection exerted by the octarepeat region against copper in vivo. In addition, the promoter region of the PrPC gene contains putative metal response elements suggesting it may be regulated by heavy metals. Here we address some of the evidence that support a physiological link between PrPC and...
Tipo: Journal article Palavras-chave: Prion protein; Copper; Heparin; HSPG; Prnp; MRE.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100005
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The specificity of interactions between proteins and sulfated polysaccharides Anais da ABC (AABC)
Mulloy,Barbara.
Sulfated polysaccharides are capable of binding with proteins at several levels of specificity. As highly acidic macromolecules, they can bind non-specifically to any basic patch on a protein surface at low ionic strength, and such interactions are not likely to be physiologically significant. On the other hand, several systems have been identified in which very specific substructures of sulfated polysaccharides confer high affinity for particular proteins; the best-known example of this is the pentasaccharide in heparin with high affinity for antithrombin, but other examples may be taken from the study of marine invertebrates: the importance of the fine structure of dermatan sulfate (DS) to its interaction with heparin cofactor II (HCII), and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sulfated polysaccharides; Fucans; Dermatan sulfate; Heparan sulfate; Heparin; Protein structure; Growth factors; Development.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000400007
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