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A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases BJM
Angelini,Leticia Mara Lima; Silva,Amanda Ribeiro Martins da; Rocco,Lucas de Freitas Coli; Milagre,Cintia Duarte de Freitas.
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitrile hydratase; Amidase; Nitrilase; Amidase inhibitor; High-throughput screening.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100113
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Development of protocol for exocellulase activity direct detection in culture medium from metagenomics library clones for high-throughput screening. Repositório Alice
MICHALCZECHEN-LACERDA, V. A.; SOUTO, B. de M.; QUIRINO, B. F..
Tipo: Separatas Palavras-chave: High-throughput screening; Exocellulase activity.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1031522
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Enzymatic potential of heterotrophic bacteria from a neutral copper mine drainage BJM
Costa,Bruna Zucoloto da; Rodrigues,Viviane Drumond; Oliveira,Valéria Maia de; Ottoboni,Laura Maria Mariscal; Marsaioli,Anita Jocelyne.
Abstract Copper mine drainages are restricted environments that have been overlooked as sources of new biocatalysts for bioremediation and organic syntheses. Therefore, this study aimed to determine the enzymatic activities (esterase, epoxide hydrolase and monooxygenase) of 56 heterotrophic bacteria isolated from a neutral copper mine drainage (Sossego Mine, Canaã dos Carajás, Brazil). Hydrolase and monooxygenase activities were detected in 75% and 20% of the evaluated bacteria, respectively. Bacterial strains with good oxidative performance were also evaluated for biotransformation of organic sulfides. Fourteen strains with good enzymatic activity were identified by 16S rRNA gene sequencing, revealing the presence of three genera: Bacillus, Pseudomonas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copper mine drainage; High-throughput screening; Hydrolases; Monooxygenases.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400846
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