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Registros recuperados: 25
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A pilot study on the identification of human papillomavirus genotypes in tongue cancer samples from a single institution in Ecuador BJMBR
Delgado Ramos,G.M.; Cotter,T.G.; Flor Ramos,L.; Torres Floril,V.; Ramos Martinez,G.A.; Ruiz-Cabezas,J.C..
The relationship between human papillomavirus (HPV) and oropharyngeal squamous cell carcinoma has been established. However, data from Ecuador is limited. The objective of this study was to characterize HPV infection in Ecuadorian patients with tongue cancer. Fifty-three patients with tongue cancer treated at the tertiary referral center Sociedad de Lucha Contra el Cancer (SOLCA), Guayaquil, between 2006 and 2011 were identified. Linear Array® HPV genotyping was used to identify the presence and types of HPV on formalin-fixed paraffin-embedded biopsy samples from these patients with tongue cancer. HPV was identified in 42% (n=22) and high-risk (HR) HPV in 17% (n=9), with 18 different HPV types identified. The most common types were the HR HPV 33 (14%) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Oropharyngeal; Oral; Ecuador; Head and neck cancer; Epidemiology.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100609
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Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology Genet. Mol. Biol.
Cassel,Ana Paula Rebelo; Barcellos,Regina Bones; Silva,Cláudia Maria Dornelles da; Almeida,Sabrina Esteves de Matos; Rossetti,Maria Lucia Rosa.
The aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16, 18, 31, 33, 39, 45 and 59 were also investigated. Of the 158 samples, 20 (12.7%) and 47 (29.7%) were positive for HPV-DNA and MN, respectively. Evidence for MN was found in 11 out of 20 (55%) HPV-DNA positive samples and in 36 out of 138 (26.1%) HPV-DNA negative ones. MN presence was significantly higher in HPV-DNA positive samples (p = 0.016). On the other hand, the absence of MN observed in a considerable number of HPV-DNA negative samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Micronucleus; Cytology; High-risk HPV; Southern Brazil.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300007
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Association between the p53 arginine/arginine homozygous genotype at codon 72 and human papillomavirus E6/E7 mRNA expression BJID
Chuery,Ana Carolina Silva; Silva,Ismael Dale Cotrim Guerreiro da; Ribalta,Julisa Chamorro Lascasas; Speck,Neila Maria de Góis.
ABSTRACT Objective: To evaluate the association between p53 polymorphisms and human papillomavirus (HPV) E6/E7 mRNA expression. Methods: We analyzed 175 cervical samples from women aged 16-69 years old who were tested for HPV E6/E7 mRNA expression (NucliSENS® EasyQ® HPV). The samples were divided into three groups: positive (n = 75) those with positive HPV E6/E7 mRNA expression and positive high-risk HPV Hybrid Capture (HR-HC) test; negative (n = 52) those with negative HPV E6/E7 mRNA expression and positive HR-HC; and control (n = 48) those with negative HPV E6/E7 mRNA expression and negative HR-HC. The p53 polymorphisms at codons 11, 72, and 248 were evaluated through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Oncoproteins; Genetic polymorphisms; P53 gene; Arginine.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300248
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Correlation of interleukin-18 gene polymorphism with the susceptibility of condyloma acuminatum in Chinese population BJID
Wang,Changyuan; Wei,Li; Chu,Weilin; Yu,Haiyang; Yu,Xinjuan; Li,Chunxia.
ABSTRACT Host immunogenetic setting is involved in the regulation of human papillomavirus (HPV) infection and development of condyloma acuminatum (CA). We investigated the correlation of two common single nucleotide polymorphisms (SNPs) (−607C/A and −137G/C) of IL-18 with the susceptibility of CA in a large Chinese cohort. Out of 408 CA patients analyzed, 300 had HPV infection transmitted through sexual contact (SC) and 108 through non-sexual contact (NSC). In addition, 360 healthy volunteers were enrolled as controls. SNPs at positions −607C/A and −137G/C in IL-18 promoter were analyzed. Comparing CA patients to healthy controls, no dominant relevance was found between the IL-18 promoter −607 C/A or −137G/C polymorphisms and the CA disease either...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Condyloma acuminatum; Human papillomavirus; Cytokine; Single nucleotide polymorphism; IL-18.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000600388
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Detection of point mutations by non-isotopic single strand conformation polymorphism BJMBR
Pinheiro,N.A.; Moura,R.P.; Monteiro,E.; Villa,L.L..
We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53. The protocol does not require any particular facilities or equipment, such as radioactive handling, large gel units for sequencing, or a semiautomated electrophoresis system. This technique consists of amplification of DNA fragments by PCR with specific oligonucleotide primers, denaturation, and electrophoresis on small neutral polyacrylamide gels, followed by silver staining. The sensitivity of this procedure is comparable to other described techniques and the method is easy to perform and applicable to a variety of tissue specimens.
Tipo: Info:eu-repo/semantics/other Palavras-chave: P53 gene; Human papillomavirus; PCR; SSCP; Cancer; Silver staining.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100008
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Epidemiological and functional implications of molecular variants of human papillomavirus BJMBR
Sichero,L.; Villa,L.L..
Human papillomavirus genomes are classified into molecular variants when they present more than 98% of similarity to the prototype sequence within the L1 gene. Comparative nucleotide sequence analyses of these viruses have elucidated some features of their phylogenetic relationship. In addition, human papillomavirus intratype variability has also been used as an important tool in epidemiological studies of viral transmission, persistence and progression to clinically relevant cervical lesions. Until the present, little has been published concerning the functional significance of molecular variants. It has been shown that nucleotide variability within the long control region leads to differences in the binding affinity of some cellular transcriptional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; DNA sequencing; Oncogenic potential; Cervical cancer; Immunity; Epidemiology.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600002
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Estrogen and progesterone receptors in human papilloma virus-related cervical neoplasia BJMBR
Coelho,F.R.G.; Prado,J.C.M.; Pereira Sobrinho,J.S.; Hamada,G.; Landman,G.; Pinto,C.A.; Nonogaki,S.; Villa,L.L..
Estrogen (ER) and progesterone (PR) receptors in the normal uterine cervix, cervical intraepithelial neoplasia and invasive carcinoma were studied in consecutive samples from Hospital do Câncer, São Paulo, between 1996 and 1997. Tissue was collected by removing a fragment of the tumoral area using a 5-mm diameter biopsy punch, followed by removal of a macroscopically normal area as close as possible from the tumor. Histopathological confirmation was obtained for all specimens analyzed. A total of 24 normal tissues, 17 cases of cervical intraepithelial neoplasia and 7 of invasive carcinomas were studied. The ER/PR ratio was determined by immunohistochemistry using monoclonal antibodies specific for each receptor. Adjacent tissue slides were submitted to...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Estrogen receptor; Progesterone receptor; Cervical cancer; Cervical intraepithelial neoplasia; Human papillomavirus.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100012
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Genetic susceptibility to HPV infection and cervical cancer BJMBR
Maciag,P.C.; Villa,L.L..
Squamous cell carcinoma of the cervix (SCCC) is one of the leading causes of death in developing countries. Infection with high-risk human papillomavirus (HPV) is the major risk factor to develop malignant lesions in the cervix. Polymorphisms of the MHC and p53 genes seem to influence the outcome of HPV infection and progression to SCCC, although controversial data have been reported. MHC are highly polymorphic genes that encode molecules involved in antigen presentation, playing a key role in immune regulation, while p53 is a tumor suppressor gene that regulates cell proliferation. The HPV E6 protein from high-risk types binds p53 and mediates its degradation by the ubiquitin pathway. The role of these polymorphisms in genetic susceptibility to HPV...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Cervical cancer; HLA; P53; Polymorphism.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700017
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High quality DNA from human papillomavirus (HPV) for PCR/RFLPs BABT
Wanderlei-Silva,Denise; Nobre,Mariana; Gonzaga,Rosa Silva; Viana,Luciana Silva; Ramalho Neto,Eduardo.
The analysis of DNA in clinical samples for a secure diagnostic has become indispensable nowadays. Techniques approaching isolation of high molecular weigth DNA of HPV could lead to efficient amplification and early clinical diagnosis of the virus DNA by PCR (polymerase chain reaction). We describe a fast, non-toxical, efficient and cheap method for DNA isolation of human papilloma virus (HPV) from cervical smears using guanidine (DNAzol solution). A 450 bp DNA band correponding to the late region (L1) of the virus genome was detected by PCR, showing that the DNAzol extraction soluction generated a good viral DNA yield. The electrophoretic pattern after digestion with restriction endonucleases (RFLPs/PCR) revealed the predominance of HPV-16 and HPV-33 in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; DNA purification; PCR; RFLP.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100006
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HPV vaccines: a controversial issue? BJMBR
Nicol,A.F.; Andrade,C.V.; Russomano,F.B.; Rodrigues,L.L.S.; Oliveira,N.S.; Provance Jr,D.W..
Controversy still exists over whether the benefits of the available HPV vaccines outweigh the risks and this has suppressed uptake of the HPV vaccines in comparison to other vaccines. Concerns about HPV vaccine safety have led some physicians, healthcare officials and parents to withhold the recommended vaccination from the target population. The most common reason for not administering the prophylactic HPV vaccines are concerns over adverse effects. The aim of this review is the assessment of peer-reviewed scientific data related to measurable outcomes from the use of HPV vaccines throughout the world with focused attention on the potential adverse effects. We found that the majority of studies continue to suggest a positive risk-benefit from vaccination...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Vaccination; Adverse events.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500301
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Human papillomavirus detection and p16 methylation pattern in a case of esophageal papilloma BJMBR
Afonso,L.A.; Moysés,N.; Cavalcanti,S.M.B..
Esophageal cancer is a prevalent cancer worldwide. Some studies have reported the possible etiology of human papillomavirus (HPV) in benign and malignant papillomas of the esophagus but the conclusions are controversial. In the present study, we investigated an esophageal papilloma from a 30-year-old male patient presenting aphasia. HPV DNA was detected by generic PCR using MY09/11 primers, and restriction fragment length polymorphism revealed the presence of HPV54, usually associated with benign genital lesions. Hypermethylation of the pINK4A gene was also investigated due to its relation to malignant transformation, but no modification was detected in the host gene. Except for an incipient reflux, no risk factors such as cigarette smoking, alcohol abuse...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Human papillomavirus; Esophageal cancer; Restriction fragment length polymorphism.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700013
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Human papillomavirus infection among sexual partners attending a Sexually Transmitted Disease Clinic in Rio de Janeiro, Brazil BJMBR
Afonso,L.A.; Rocha,W.M.; Carestiato,F.N.; Dobao,E.A.; Pesca,L.F.; Passos,M.R.L.; Cavalcanti,S.M.B..
Cervical cancer is a major source of illness and death among women worldwide and genital infection with oncogenic human papillomavirus (HPV) its principal cause. There is evidence of the influence of the male factor in the development of cervical neoplasia. Nevertheless, the pathogenic processes of HPV in men are still poorly understood. It has been observed that different HPV types can be found among couples. The objective of the present study was to investigate HPV infections in female patients (n = 60 females/group) as well as in their sexual partners and to identify the concordance of HPV genotypes among them. By using the polymerase chain reaction, we detected a 95% prevalence of HPV DNA in women with cervical intraepithelial neoplasia (CIN) compared...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Neoplasia; Couples; Sexual partners; Polymerase chain reaction.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600533
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Human papillomavirus infection and its association with cervical dysplasia in Ecuadorian women attending a private cancer screening clinic BJMBR
Brown,C.R.; Leon,M.L.; Muñoz,K.; Fagioni,A.; Amador,L.G.; Frain,B.; Tu,W.; Qadadri,B.; Brown,D.R..
Women living in Latin American countries bear a disproportionate burden of cervical cancer, a condition caused by infection with the human papillomavirus (HPV). We performed a study in Santa Elena, Guayas (currently Santa Elena Province), Ecuador, to determine how often HPV could be detected in women attending a private cancer screening clinic. Participants underwent a Pap test, and vaginal and cervical swabs were performed for HPV testing by the polymerase chain reaction (PCR). Each participant completed a verbally administered survey. The mean age of 302 participants was 37.7 years (range 18 to 78 years). The majority of cervical and vaginal specimens contained sufficient DNA to perform PCR. Overall, 24.2% of the participants had either a cervical or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Cervical dysplasia; Ecuador.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700007
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Human papillomavirus infection and p53 protein expression in vulvar intraepithelial neoplasia and invasive squamous cell carcinoma BJMBR
Engelman,D.E.S.; Andrade,L.A.L.A.; Vassallo,J..
The etiopathogenesis of vulvar intraepithelial neoplasia (VIN III) and invasive squamous cell carcinoma are largely unknown. Since there are few studies on Brazilian patients, our purpose was to determine the frequency of human papillomavirus (HPV) infection and the expression of p53 in these lesions, and associate them with other factors such as age, morphological subtypes, multicentric and multifocal disease. Thirty-eight cases of VIN III, nine of superficially invasive carcinoma, and 55 of invasive vulvar carcinoma were retrospectively evaluated from 1983 to 1995 for the presence of HPV by immunohistochemistry and in situ hybridization, and for p53 protein expression by immunohistochemistry on paraffin sections. All cases for whom material (slides and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vulvar intraepithelial neoplasia; Vulvar carcinoma; Human papillomavirus; P53 protein.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900003
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Human papillomavirus infection in women attended at a cervical cancer screening service in Natal, Brazil BJM
Fernandes,Thales Allyrio Araújo de Medeiros; Meissner,Rosely de Vasconcellos; Bezerra,Laelson Freire; Azevedo,Paulo Roberto Medeiros de; Fernandes,José Veríssimo.
We analyzed cervical specimens of 202 women, aged 15 to 64 years, attended at Luis Antonio Hospital, Natal, Brazil, to determine the prevalence of HPV and identify the more frequent genotypes and risk factors for HPV infection in women attended at a cervical cancer screening service. Two specimens were collected from each patient: one for cytological examination and the other to detect HPV DNA by PCR, and typing by dot blot hybridization. A total of 54.5% of the sample had normal cytology and 45.5% had cytological alterations. HPV was detected in 24.5% of the cytologically normal women and in 59.8% of those with altered cytology. Both single and double HPV infection increased the likelihood of cytological alterations. Thirteen types of HPV were identified,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Cervical cancer; Epidemiology; Prevalence; Risk factors.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300031
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Human papillomavirus infection in women from Tlaxcala, Mexico BJM
Velázquez-Márquez,Noé; Jiménez-Aranda,Lucio Jaime; Sánchez-Alonso,Patricia; Santos-López,Gerardo; Reyes-Leyva,Julio; Vallejo-Ruiz,Verónica.
Cervical cancer is an important health problem in women living in developing countries. Infection with some genotypes of human papillomavirus (HPV) is the most important risk factor associated with cervical cancer. Little information exists about HPV genotype distribution in rural and suburban regions of Mexico. Thus, we determined the prevalence of HPV genotypes in women from Tlaxcala, one of the poorest states in central Mexico, and we evaluated age infection prevalence and risk factors associated with cervical neoplasm. A cross-sectional study was conducted in 236 women seeking gynecological care at the Mexican Institute for Social Security in Tlaxcala, Mexico. Cervical scrapings were diagnosed as normal, low-grade, and high-grade squamous...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epidemiology; Human papillomavirus; Mexico; PCR; Squamous intraepithelial lesions.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300027
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Impact of HPV infection on the development of head and neck cancer BJMBR
Betiol,J.; Villa,L.L.; Sichero,L..
Human papillomavirus (HPV)-related head and neck squamous cell carcinoma (HNSCC) is considered to be a distinct clinical entity with better prognosis than the classical tobacco- and alcohol-associated tumors. The increasing incidence of this neoplasia during the last decades highlights the need to better understand the role of HPV in the development of these cancers. Although the proportion of HNSCC attributed to HPV varies considerably according to anatomical site, overall approximately 25% of all HNSCC are HPV-DNA positive, and HPV-16 is by far the most prevalent type. In this review we discuss the existing evidence for a causal association between HPV infection and HNSCC at diverse anatomical head and neck subsites.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Oncogenic potential; Head and neck cancer; Viral prevalence.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300217
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L1 sequence of a new human papillomavirus type-58 variant associated with cervical intraepithelial neoplasia BJMBR
Veras,V.S.; Cerqueira,D.M.; Martins,C.R.F..
The present study on molecular characterization of a human papillomavirus (HPV) isolated in Central Brazil describes the L1 gene sequence from a new variant of HPV-58, the isolate Bsb-02. The sample was from a smear obtained from a woman with cervical intraepithelial neoplasia grade II. The whole L1 gene from isolate Bsb-02 was sequenced automatically, showing 99.1% nucleotide identity with the gene from the HPV-58 reference. The clustering between Bsb-02 and HPV-58 reference sequence was also supported by phylogenetic analysis. Fourteen nucleotide substitutions were observed: eight were synonymous and six were associated with amino acid substitutions. A10V and V144I have not been previously described. At GenBank, the only complete L1 sequence from HPV-58...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Human papillomavirus; HPV-58; L1 gene; Variants.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100001
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Lack of detection of human papillomavirus DNA in prostate carcinomas in patients from northeastern Brazil Genet. Mol. Biol.
Araujo-Neto,Ari P.; Ferreira-Fernandes,Hygor; Amaral,Carolina M.M.; Santos,Lina G.; Freitas,Antônio C.; Silva-Neto,Jacinto C.; Rey,Juan A.; Burbano,Rommel R.; Silva,Benedito B. da; Yoshioka,France K.N.; Pinto,Giovanny R..
Abstract Prostate cancer is the second most common cancer among men in western populations, and despite its high mortality, its etiology remains unknown. Inflammatory processes are related to the etiology of various types of tumors, and prostate inflammation, in particular, has been associated with prostate cancer carcinogenesis and progression. Human papillomavirus (HPV) is associated with benign and malignant lesions in the anogenital tract of both females and males. The possible role of HPV in prostate carcinogenesis is a subject of great controversy. In this study, we aimed to examine the prevalence of HPV infections in prostate carcinomas of patients from northeastern Brazil. This study included 104 tissue samples from primary prostate carcinoma...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Prostate cancer; Prevalence; HPV PCR detection; Molecular epidemiology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100024
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Multiple types of human papillomavirus in cervical samples in women in Campo Grande, MS, Brazil BJID
Tozetti,Inês Aparecida; Scapulatempo,Ilzia Doraci Lins; Kawski,Vicky Liege; Ferreira,Antonio Walter; Levi,José Eduardo.
We analyzed 87 cervical samples from Campo Grande, Mato Grosso do Sul, with a PGMY/GP+ nested PCR system. Positive samples were typed using E7 type-specific primer pairs for HPV 6/11, 16, 18, 45 and 66. Eighteen samples (22%) were infected with HPV6/11, 18 samples (22%) with HPV66, 13 samples (15.9%) with HPV45, 8 samples (9.8%) with HPV18 and 7 samples (8.5%) with HPV16. Seventeen samples (20.7%) were infected by two HPV types, and five samples (6.1%) by three HPV types. We conclude that infection with multiple types is present at a high frequency in our population and that there is a relation between some types and cytological finds.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; Multiple types; Nested PCR; TS-PCR.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500001
Registros recuperados: 25
Primeira ... 12 ... Última
 

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