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Detection and identification of huwentoxin-IV interacting proteins by biotin-avidin chemistry combined with mass spectrometry J. Venom. Anim. Toxins incl. Trop. Dis.
Yu,Hai; Liu,Hui; Yan,Yizhong; Duan,Zhigui; Wang,Xianchun.
Background : Numerous spider toxins are of interest as tools for neurophysiological research or as lead molecules for the development of pharmaceuticals and insecticides. Direct detection and identification of the interacting proteins of a spider toxin are helpful for its action-mechanism analysis and practical application. The present study employed a combinative strategy for the analysis of interacting proteins of huwentoxin-IV (HWTX-IV), a peptidic neurotoxin from the venom of the spiderSelenocosmia huwena.Results : HWTX-IV was first lightly labeled with biotin under the optimized mild experimental conditions and the toxin labeled with a single biotin group (monobiotinylated HWTX-IV) was demonstrated by electrophysiological experiments to retain its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotin labeling; Huwentoxin-IV; Far-western blotting; Interacting protein; Mass spectrometry.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200328
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