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A comparison of dual-functional whey hydrolysates by the use of commercial proteases Ciênc. Tecnol. Aliment.
ROSA,Luísa Ozorio Lopes da; SANTANA,Marcelo Cerutti; AVEZEDO,Tatiana Lima; BRÍGIDA,Ana Iraidy Santa; GODOY,Ronoel; PACHECO,Sidney; MELLINGER-SILVA,Caroline; CABRAL,Lourdes Maria Correa.
Abstract Whey is well-known for the functional and bioactive properties of its proteins and peptides, which are of great interest to food and nutraceutical industries. The aim of this study was to investigate the effect of four different commercial proteases (Novo ProD® (NPD), Alcalase® (ALC), Pancreas Trypsin® (TRY), and Flavourzyme® (FLA)) in the generation of hydrolysates with emulsifying and antioxidant activities. Hydrolysis processes were carried out for 5 h, reaching maximum degrees of 18.5, 15.5, 9.2, and 8.7% for NPD, ALC, TRY, and FLA, respectively. All tested enzymes generated very diverse, but conservative peptide profiles when comparing the treatments along the time, with the main enzymatic actions up to 120 min-reaction. An increase in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Whey protein; Hydrolysis; Functionality; Emulsification; Antioxidant activity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000500031
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Acid and enzymatic hydrolysis to recover reducing sugars from cassava bagasse: an economic study BABT
Woiciechowski,Adenise Lorenci; Nitsche,Saul; Pandey,Ashok; Soccol,Carlos Ricardo.
The objective of this work was to study the acid and enzymatic hydrolysis of cassava bagasse for the recovery of reducing sugars and to establish the operational costs. A statistical program "Statistica", based on the surface response was used to optimize the recovery of reducing sugars in both the processes. The process economics was determined considering the values of reducing sugars obtained at laboratory scale, and the operations costs of a cylindrical reactor of 1500 L, with flat walls at the top and bottom. The reactor was operated with 150 kg of cassava bagasse and 1350 kg of water. The yield of the acid hydrolysis was 62.4 g of reducing sugars from 100 g of cassava bagasse containing 66% starch. It represented 94.5% of reducing sugar recovery. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cassava bagasse; Hydrolysis; Acid; Enzyme; Reducing sugars; Economics.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300018
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Alkali pretreated of wheat straw and its enzymatic hydrolysis BJM
Han,Lirong; Feng,Juntao; Zhang,Shuangxi; Ma,Zhiqing; Wang,Yonghong; Zhang,Xing.
The efficiency of enzymatic hydrolysis of cellulose can be improved by various pretreatments of the substrate. In order to increase the efficiency of enzymatic saccharification of the wheat straw, we determined the effect of different pretreatments on the physical structure, chemical components and enzymatic saccharification of wheat straw. Our results showed that combination of grinding and sodium hydroxide (NaOH) treatment had high effect on the enzymatic hydrolysis of wheat straws. The optimal pretreatment condition was to grind the wheat straws into the sizes of 120 meshes followed by treatment with 1.0% NaOH for 1.5 h (121°C/15psi). Under this condition, the cellulose content of wheat straw was increased by 44.52%, while the content of hemicellulose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Cellulose; Fermentation; Hydrolysis; Lignocellulosic; Pretreatment.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100006
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Anaerobic biodegradability of dairy wastewater pretreated with porcine pancreas lipase BABT
Mendes,Adriano Aguiar; Pereira,Ernandes Benedito; Furigo Jr.,Agenor; Castro,Heizir Ferreira de.
Lipids-rich wastewater was partial hydrolyzed with porcine pancreas lipase and the efficiency of the enzymatic pretreatment was verified by the comparative biodegradability tests (crude and treated wastewater). Alternatively, simultaneous run was carried out in which hydrolysis and digestion was performed in the same reactor. Wastewater from dairy industries and low cost lipase preparation at two concentrations (0.05 and 0.5% w.v-1) were used. All the samples pretreated with enzyme showed a positive effect on organic matter removal (Chemical Oxygen Demand-COD) and formation of methane. The best results were obtained when hydrolysis and biodegradation were performed simultaneously, attaining high COD and color removal independent of the lipase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradability; Hydrolysis; Lipases; Lipids-rich wastewater and biogas.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600003
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Bebida saborizada elaborada con leche de soya parcialmente hidrolizada OceanDocs
Ruiz, A.; Mejías, E.; González, J.; Silveira, I.; Cárdenas, M. E.; Padrón, X..
Mediante la acción de la papaína se puede obtener un hidrolizado parcial de la leche de soya en la que se superan algunas deficiencias organolépticas inherentes al frijol de soya o al proceso de obtención de la misma, aún así para su consumo debe ofrecerse bajo la forma de una bebida saborizada. Para formular dicha bebida se emplearon cuatro saborizantes: cocoa micropulverizada (CHC), sabor chocolate microencapsulado en polvo (CHP), sabor chocolate líquido (CHL) y sabor café microencapsulado en polvo (SCP); como endulzantes se emplearon azúcar, aspartame y una mezcla 50 % de aspartame + acesulfame. Se tomaron como variables independientes la cantidad de saborizantes y la de edulcorantes que se combinaron según sendos planes factoriales 22 con punto...
Tipo: Journal Contribution Palavras-chave: Hydrolysis; Food processing; Palatability.
Ano: 2008 URL: http://hdl.handle.net/1834/4703
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Bioethanol from Microalgal Biomass: A Promising Approach in Biorefinery BABT
Silva,Carlos Eduardo de Farias; Bertucco,Alberto.
Abstract The development of new technologies which increase the production of biofuel without directly compete with food production is required. Microalgal biomass has recently been in the highlight. The role of this biomass is here discussed within the concept of biorefinery and industrial sustainability of bioethanol production. The process of cultivation in order to accumulate around 50% of carbohydrates in the biomass (dry weight) and the importance of water and nutrient recycling are reviewed. Saccharification of biomass using enzymes or acids and alternative processes such as hydrothermal liquefaction and flash hydrolysis are addressed. Since the main monosaccharide in microalgal biomass is glucose, high rates of hydrolysis and fermentation were,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethanol; Microalgae; Biofuel; Hydrolysis; Nutrient recycle.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100602
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Changes in proximate composition and lipid quality of atherina atherina sp. during sun drying process OceanDocs
Selmi, S.; Bouriga, N.; Faure, E.; Trabelsi, M..
Dans le présent travail, l’effet du séchage solaire sur la composition biochimique et la qualité des lipides des athérines a été étudié. La teneur en lipides, protéines et en cendres dans l’athérine a augmenté significativement (p <0.05) après le séchage. Cependant, celle de l’humidité a montré une forte diminution pour atteindre 22.37% dans le produit final. D’autre part, l’étude des indicateurs d’altération des lipides (Oxydation et Hydrolyse) nous a montré que la qualité des lipides des athérines est affectée par ce type de traitement. En effet, les taux des hydropéroxydes et des acides gras libres ont augmenté significativement (p < 0.05) après le séchage pour atteindre respectivement 2.02 meq active O2/kg huile et 3.67%. La formation des...
Tipo: Journal Contribution Palavras-chave: Biochemical composition; Dried products; Drying; Hydrolysis; Methodology; Oxidation; Processing fishery products; Quality assurance; Atherina; Http://aims.fao.org/aos/agrovoc/c_688.
Ano: 2008 URL: http://hdl.handle.net/1834/4152
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Chemical composition and dry matter digestibility of sugar cane oxide treated with calcium Arq. Bras. Med. Vet. Zootec.
Romão,C.O.; Carvalho,G.G.P.; Leite,V.M.; Santos,A.S.; Chagas,D.M.T.; Ribeiro,O.L.; Oliveira,P.A.; Magalhães,A.F.; Pires,A.J.V..
The aim of this study was to identify the most adequate level of calcium oxide (CaO) in the treatment of sugar cane by evaluating the chemical composition and in vitro digestibility of dry matter. The sugar cane was homogenized with CaO levels 0, 0.75, 1.5, 2.25, 3.0, 3.75 and 4.5%, in natura matter, for 24 hours. The dry matter and mineral matter increased, while the organic matter of the sugar cane decreased (P <0.05) linearly as a result of the CaO levels. The CaO had no effect on the levels of crude protein and ether extract. The phosphorus concentration remained unchanged, but there was a significant increase (P<0.05) in the calcium content. The contents of neutral detergent fiber, acid detergent fiber and cellulose decreased linearly with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Additive; Forage; Hydrolysis; Saccharum; Alkaline treatment.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200029
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Comparative analysis of enzymatic activity of tannase in non-conventional yeasts to produce ellagic acid Ciênc. Tecnol. Aliment.
Márquez-López,Anahí; Ramírez-Conejo,Juan David; Chávez-Parga,Ma. Del Carmen; Flores,Dora Cecilia Valencia; Jaramillo,Miguel Angel Zamudio; Rodríguez,Horacio González; González-Hernández,Juan Carlos.
Abstract Non-conventional yeasts represent an alternative for microorganisms that are capable to produce the enzyme Tannase (EC 3.1.1.20), which has many applications is the food and pharmaceutical industry as it stands out for its ability to produce ellagic acid out of the enzymatic hydrolysis of ellagitannins. Ellagic acid has been studied in the medical area for its action against various diseases, as is the case of cancer or heart problems. The investigation was conducted to evaluate seven non-conventional yeasts - Debaryomyces hansenii PYC ISA 1510, Debaryomyces hansenii PYC 2968, Candida parapsilosis, Candida utilis, Pichia pastoris, Pichia kluyveri, Issatchenkia terricola - as producers of the enzyme tannase. This was done in mediums of fermentation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Non-conventional yeasts; Enzymatic activity; Tannase; Ellagic acid; Hydrolysis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005031111
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Consolidated briefing of biochemical ethanol production from lignocellulosic biomass Electron. J. Biotechnol.
Spyridon,Achinas; Willem Euverink,Gerrit Jan.
Bioethanol production is one pathway for crude oil reduction and environmental compliance. Bioethanol can be used as fuel with significant characteristics like high octane number, low cetane number and high heat of vaporization. Its main drawbacks are the corrosiveness, low flame luminosity, lower vapor pressure, miscibility with water, and toxicity to ecosystems. One crucial problem with bioethanol fuel is the availability of raw materials. The supply of feedstocks for bioethanol production can vary season to season and depends on geographic locations. Lignocellulosic biomass, such as forest-based woody materials, agricultural residues and municipal waste, is prominent feedstock for bioethanol cause of its high availability and low cost, even though the...
Tipo: Journal article Palavras-chave: Bioconversion; Bioethanol; Fermentation; Hydrolysis; Renewable fuels.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500009
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De l'utilisation de protéases industrielles pour la production de sauces de poisson ArchiMer
Le, Minh Chau.
Fish sauces are traditionally used in most Asian countries. Whatever the process, the principle is the same i.e. a fish autolysis in saline media followed by a fermentation step. Not less than 18 months are needed to obtain a brown liquid rich in salt and amino acids. This PhD work aims to determine if an addition of proteases can speed up the process while keeping the nutritional and sensorial properties. The first chapter presents the characteristics of fish sauces and the key parameters of the processes used. In the second chapter, several industrial proteases are compared each other for their capacity to liquefy the fishes in hyper saline conditions. Kinetics of proteolysis and biochemical characteristics of the resulting hydrolysates are established...
Tipo: Text Palavras-chave: Sauce de poisson; Hydrolyse; Enzyme; Protéase; Sardine; Anchois; Fish sauce; Hydrolysis; Enzyme; Protease; Sardine; Anchovy.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00259/36997/35488.pdf
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Degradation of Technora aramid fibres in alkaline and neutral environments ArchiMer
Derombise, Guillaume; Van Schoors, Laetitia Vouyovitch; Davies, Peter.
Technora fibres are high performance aramid fibres which have been used in ropes and protective clothing for many years. They are also now being proposed as geotextiles for soil reinforcement. However, there is a lack of experience on the long-term behaviour of Technora fibres in an alkaline environment (lime-treated ground.). Consequently, aging studies have been performed under different conditions (deionised water, pH9 and pH11). Hydrolytic degradation has been evaluated by FTIR, viscometry, TGA, density measurements, SEM, and by tensile measurements. Some chain degradation and finish rearrangements have been highlighted, but Technora fibres retain their mechanical properties for all the conditions considered here.
Tipo: Text Palavras-chave: Aramids; Fibres; Technora; Hydrolysis; Long-term behaviour.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00002/11289/8052.pdf
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Description and modeling of polyurethane hydrolysis used as thermal insulation in oil offshore conditions ArchiMer
Le Gac, Pierre-yves; Choqueuse, Dominique; Melot, D..
Polymers are widely used for passive thermal insulation coatings on steel pipe in offshore oil and gas production. In this industry, structures used in deep sea have to be reliable, as they are in service for more than 20 years in a very severe environment: sea water, hydrostatic pressure and temperature gradient. One of the main questions is how to test and predict the lifetime of such structures in the laboratory? This study presents one approach that has been developed to characterize and predict the degradation of polymers used as thermal insulation materials. This paper is dedicated to polyurethane (polyether based) degradation in sea water at high temperature. Ageing has been performed in natural sea water under hydrostatic pressure at temperatures...
Tipo: Text Palavras-chave: Polyurethane; Hydrolysis; Accelerated ageing; Sea water; Coating; Offshore.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00160/27132/25330.pdf
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Effect of intraperitoneally administered hydrolyzed whey protein on blood pressure and renal sodium handling in awake spontaneously hypertensive rats BJMBR
Costa,E.L.; Almeida,A.R.; Netto,F.M.; Gontijo,J.A.R..
The present study evaluated the acute effect of the intraperitoneal (ip) administration of a whey protein hydrolysate (WPH) on systolic arterial blood pressure (SBP) and renal sodium handling by conscious spontaneously hypertensive rats (SHR). The ip administration of WPH in a volume of 1 ml dose-dependently lowered the SBP in SHR 2 h after administration at doses of 0.5 g/kg (0.15 M NaCl: 188.5 ± 9.3 mmHg vs WPH: 176.6 ± 4.9 mmHg, N = 8, P = 0.001) and 1.0 g/kg (0.15 M NaCl: 188.5 ± 9.3 mmHg vs WPH: 163.8 ± 5.9 mmHg, N = 8, P = 0.0018). Creatinine clearance decreased significantly (P = 0.0084) in the WPH-treated group (326 ± 67 µL min-1 100 g body weight-1) compared to 0.15 M NaCl-treated (890 ± 26 µL min-1 100 g body weight-1) and captopril-treated (903...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arterial blood pressure; Hydrolysis; Renal function; Urinary sodium excretion; Whey protein isolate.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200010
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Effect of ultrasonication of Switchgrass on fermentable sugar production and biomass physical structure CIGR Journal
Sharma, Rajat; Sharma-Shivappa, Ratna R.; Boyette, Michael D.; Stikeleather, Larry F..
Pretreatment of lignocellulosic biomass for enhancing sugar yields has been studied extensively over the years.  Conventional methods employed to preprocess biomass to make it conducive for enzymatic hydrolysis have been hampered by issues like poor energy efficiency and production of undesirable by-products.  Ultrasonication, which involves the treatment of biomass through ultrasonic sound waves in a liquid medium without additional chemicals, is believed to have potential for biomass pretreatment.  In this study the effects of ultrasonication on switchgrass, a potential feedstock for bioethanol production due to its high cellulosic content, were investigated.  Results of compositional analysis and scanning electron microscopy conducted to visualize...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Panicum virgatum; Amplitude; Pretreatment; Hydrolysis; Scanning electron microscopy.
Ano: 2013 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2549
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Effect on the enzymatic hydrolysis of lipids from dairy wastewater by replacing Gum Arabic emulsifier for sodium chloride BABT
Mendes,Adriano Aguiar; Castro,Heizir Ferreira de.
The objective of this work was to evaluate the replacement of Gum Arabic for sodium chloride to reduce fat and organic contents in dairy wastewater using two low cost commercially available lipase preparations from animal source (Kin Master - LKM and Nuclear- LNU). The best performance was achieved when lipase Nuclear (LNU) was used as catalyst. In addition, this lipase preparation has also lower cost, which makes its use a quite promising technique for reduction of suspended solids as proteins and lipids contents found in wastewater generated by dairy industries.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipases; Hydrolysis; Emulsifier; Biodegradability.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400017
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Enzymatic hydrolysis of sugarcane bagasse pretreated with acid or alkali BABT
Pietrobon,Vivian Cristina; Monteiro,Regina Teresa Rosim; Pompeu,Georgia Bertoni; Borges,Eduardo Poggi e; Lopes,Mario Lucio; Amorim,Henrique Vianna de; Cruz,Sandra Helena da; Viégas,Ellen Karine Diniz.
The aim of this study was to evaluate the performance of enzymatic hydrolysis of acid or alkali pretreated sugarcane bagasse for the production of fermentable sugars. The first step consisted of selection of commercial enzymes presenting the highest cellulolytic activities. After selection of four enzymes: HPL, CL, P1 and P4, their performances were tested in the bagasse pretreated with acid and alkali. The sugar content of the hydrolysates was analyzed by anion exchange liquid chromatography. Data showed that the joint action of 0.5% acid pretreatment, 121ºC, 30 minutes and enzyme CL provides the best results, 67.25 g of hexose and 148.13g of pentose per kg of dry bagasse.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bagasse sugarcane; Hydrolysis; Cellulolytic enzymes; HPAEC.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200002
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Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil BABT
Nwagu,Tochukwu Nwamaka; Okolo,Bartholomew Ndubuisi.
In this work, an α- amylase producing Fusarium sp. was isolated from the soil at 50 0C. Growth and enzyme production occurred at 30, 45 and 55 ºC. Soybean meal at 1% concentration, supplemented with 0.2% NH4Cl and 2.5% corn starch elicited the highest amylase yield. Optimum pH for the enzyme was pH 6.5 which retained over 60% of its activity after 24 h incubation at the pH range of 4.5-7.0. The enzyme showed high activity from 40-70 0C with optimal activity at 50 ºC and 78% activity was retained after incubation at 70 ºC for 30min. Catalytic function of the crude amylase was stimulated by Mg (136%), Ca (118%) and Zn (118%) at 2mM concentrations. The enzyme hydrolyzed cassava, potato and yam starches effectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydrolysis; Starch; Fungal growth; Amylase synthesis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400002
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Hydrolysis of insoluble fish protein residue from whitemouth croaker (Micropogonias furnieri) by fungi BABT
Martins,Vilásia Guimarães; Palezi,Simone Canabarro; Costa,Jorge Alberto Vieira; Prentice,Carlos.
A significant amount of insoluble fibrous protein, in the form of feather, hair, scales, skin and others are available as co-products of agro industrial processing. These wastes are rich in keratin and collagen. This study evaluated different fungi for the hydrolysis of insoluble fish protein residues. Proteins resulting from Micropogonias furnieri wastes through pH-shifting process were dried and milled for fermentation for 96 h. This resulted the production of keratinolytic enzymes in the medium. Trichoderma sp. on alkaline substrate (28.99 U mL-1) and Penicillium sp. on acidic substrate (31.20 U mL-1) showed the highest proteolytic activities. Penicillium sp. showed the largest free amino acid solubilization (0.146 mg mL-1) and Fusarium sp. the highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish waste; Fungi; Fibrous proteins; Hydrolysis; Whitemouth croaker.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100014
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Hydrolysis of whey lactose by immobilized β-Galactosidase BABT
Mariotti,Marcela Panaro; Yamanaka,Hideko; Araujo,Angela Regina; Trevisan,Henrique Celso.
Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-galactosidase; Immobilization; Lactase; Whey; Lactose; Hydrolysis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019
Registros recuperados: 41
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