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Registros recuperados: 11
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Agrowaste-based Polyhydroxyalkanoate (PHA) production using hydrolytic potential of Bacillus thuringiensis IAM 12077 BABT
Gowda,Vaishnavi; Shivakumar,Srividya.
The study identified the innate enzymatic potential (amylase) of the PHB producing strain B.thuringiensis IAM 12077 and explored the same for cost-effective production of PHB using agrowastes, eliminating the need for pretreatment (acid hydrolysis and/or commercial enzyme). Comparative polyhydroxyalkanoate (PHA) production by B. thuringiensis IAM 12077 in biphasic growth conditions using glucose and starch showed appreciable levels of growth (5.7 and 6.8 g/L) and PHA production (58.5 and 41.5%) with a PHA yield of 3.3 and 2.8 g/L, respectively. Nitrogen deficiency supported maximum PHA yield (2.46 g/L) and accumulation (53.3%). Maximum growth (3.6 g/L), PHB yield (2.6 g/L) and PHA accumulation (72.8%) was obtained with C:N ratio of 8:1 using starch as the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis IAM 12077; Polyhydroxybutyrate; Nutrient limitation; Hydrolytic enzymes; Mango peel.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100009
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Anti-fungal potential of ozone against some dermatophytes BJM
Ouf,Salama A.; Moussa,Tarek A.; Abd-Elmegeed,Alshimaa M.; Eltahlawy,Samar R..
ABSTRACT Dermatophytes are classified in three genera, Epidermophyton, Microsporum and Trichophyton. They have the capacity to invade keratinized tissue to produce a cutaneous infection known as dermatophytoses. This investigation was performed to study the effect of gaseous ozone and ozonized oil on three specific properties of six different dermatophytes. These properties included sporulation, mycelia leakage of sugar and nutrients and the activity of their hydrolytic enzymes. Generally, ozonized oil was found to be more efficacious than gaseous ozone. Microsporum gypseum and Microsporum canis were the most susceptible, while Trichophyton interdigitale and T. mentagrophytes were relatively resistant. The study revealed a steady decline in spore...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dermatophytes; Dermatophytosis; Ozone; Hydrolytic enzymes; Ozonized oil.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300697
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Evaluation of hollocelulase production by Lentinula edodes (Berk.) Pegler during the submerged fermentation growth using RSM. Repositório Alice
CHICATTO, J. A.; COSTA, A.; NUNES, H.; HELM, C. V.; TAVARES, L. B. B..
As celulases são proteínas de grande importância na hidrólise enzimática de biomassa florestal. No entanto, seu custo elevado tem estimulado o estudo de processos de obtenção de enzimas celulolíticas por fungos filamentosos, tais como os basidiomicetos que apresentam propriedades bioquímicas para degradação de material lignocelulósico. O objetivo deste trabalho foi avaliar os efeitos do teor inicial de nitrogênio (sulfato de amônia) e de um indutor de enzimas hidrolíticas (bagaço de cana de açúcar) na produção de xilanases e celulases utilizando três isolados de Lentinula edodes (Berk.) Pegler como agente de transformação. Foi realizado um planejamento fatorial 22 com repetição no ponto central, variando as concentrações de sulfato de amônia e bagaço de...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Basidiomiceto; Bagaço de cana; Sulfato de amônia; Enzima hidrolítica; Proteina; Basidiomycetes; Sugar cane bagasse; Ammonium sulfate; Hydrolytic enzymes; Proteins.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/994600
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Indução de resistência sistêmica à antracnose em feijoeiro-comum pela raça delta avirulenta de Colletotrichum lindemuthianum. Repositório Alice
CAMPOS, A.D.; HAMPE, M.M.V; FERREIRA, A.G.; ANTUNES, I.F.; CASTRO, L.A.S. de..
O objetivo deste trabalho foi avaliar o potencial da raça delta avirulenta do fungo Colletotrichum lindemuthianum, como protetora contra raças virulentas deste fungo e quanto à capacidade de induzir resistência sistêmica em feijoeiro-comum (Phaseolus vulgaris). Quatro cultivares de feijoeiro foram avaliadas quanto às alterações nas atividades de beta-1,3-glucanase e quitinase, em dois estádios de desenvolvimento (V2 e R6), três dias após a aplicação de suspensão de esporos de C. lindemuthianum raça delta avirulenta, em comparação com aplicações de água e ácido salicílico. As plantas foram, então, infectadas com o patótipo virulento 33/95 de C. lindemuthianum em suspensão e, depois de cinco dias, foram reavaliadas quanto à atividade das enzimas....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Phaseolus vulgaris; Ácido salicílico; Beta-1 3-glucanase; Controle biológico; Enzimas hidrolíticas; Quitinase; Salicylic acid; Biocontrol; Hydrolytic enzymes; Chitinase; Glucanase.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/125731
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Malting of preharvest sprouted wheat Rev. Ciênc. Agron.
Carvalho,Paulo de Tarso; Beléia,Adelaide Del Pino.
ABSTRACT Pre-harvest sprouting is one of the most important problems of the wheat crop and it is necessary to find alternative uses for the grain because millers reject the damaged wheat. In the field these grains were stimulated to germinate, due to weather conditions and their metabolic activity was enhanced, the hydrolytic enzymes promoted changes in the proteins and reserve carbohydrates, which reflect on the flour quality. Similar to malting germination has started, but with different intensity and under different conditions. There are some studies about malting of sprouted barley and the malting of rain-damaged wheat could be a possibility worth investigation. The objective of this work was to evaluate the malting of pre-harvest sprouted wheat...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Malt quality; Hydrolytic enzymes.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000400571
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Production of hydrolytic enzymes by the plant pathogenic fungus Myrothecium verrucaria in submerged cultures BJM
Moreira,Fabiana Guillen; Reis,Simone dos; Costa,Maria Aparecida Ferreira; Souza,Cristina Giatti Marques de; Peralta,Rosane Marina.
The capability of the plant pathogenic fungus Myrothecium verrucaria to produce extracellular hydrolytic enzymes in submerged cultures was studied using several substrates. The fungus was able to produce different depolymerases and glycosidases, being xylanase, pectinase and protease the most important. Lipase was found in cultures developed in the presence of olive oil, while protease activity was detected in all cultures. Xylanase and pectinase were optimally active at pH 4.5-5.5, while protease was active in a large range of pH 3.5 to 11.0. All three enzymes were maximally active at 40ºC and they were stable for several hours at temperature up to 50ºC.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrases; Hydrolytic enzymes; Myrothecium verrucaria; Protease.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100002
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Prospecting microrganisms for biofuel production prospect of microrganisms for biofuel production. Repositório Alice
BOMBA, J. P.; MELO, I. G.; OLIVEIRA, J. C. M. D.; REIS, D. P.; OLIVEIRA, C. A.; MARRIEL, I. E..
bitstream/item/166022/1/Prospecting-microrganisms.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Hydrolytic enzymes; Cellulase; Xilanase; Enzima; Amilase; Celulase; Lipase; Amylases; Xylanases.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1078759
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Selection of Cellulolytic Fungi Isolated from Diverse Substrates. Repositório Alice
DAMASO, M. C. T.; TERZI, S. da C.; FARIAS A. X.; OLIVEIRA; A. C. P.; FRAGA; M.E.; COURI. S..
2012
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cellulases; Semi-solid fermentation; Filamentous fungi; Hydrolytic enzymes.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/952275
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Selection of cellulolytic fungi isolated from diverse substrates BABT
Damaso,Mônica Caramez Triches; Terzi,Selma da Costa; Farias,Antonio Xavier; Oliveira,Ana Carolina Pereira de; Fraga,Marcelo Elias; Couri,Sonia.
The aim of the present work was to select filamentous fungi isolated from diverse substrates to obtain the strains with potential to produce the hydrolytic enzymes. From a total of 215 strains, seven strains from the soils, six from the plants and one from sugarcane bagasse were selected and identified as belonging to the Trichoderma, Penicillium and Aspergillus genera. The best hydrolytic activities obtained by semi-solid fermentation using these strains were approximately: 35; 1; 160; 170 and 120 U/gdm (CMCase, FPase, β-glucosidase, xylanase and polygalacturonase, respectively), demonstrating their potential to synthesize the enzymes compared with the results reported in the literature.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulases; Semi-solid fermentation; Filamentous fungi; Hydrolytic enzymes.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400005
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Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils BJM
Aanniz,Tarik; Ouadghiri,Mouna; Melloul,Marouane; Swings,Jean; Elfahime,Elmostafa; Ibijbijen,Jamal; Ismaili,Mohamed; Amar,Mohamed.
The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the genus Bacillus (97.5%) represented by B. licheniformis (119), B. aerius (44), B. sonorensis (33), B. subtilis (subsp. spizizenii (2) and subsp. inaquosurum (6)), B. amyloliquefaciens (subsp. amyloliquefaciens (4) and subsp. plantarum (4)), B. tequilensis (3), B. pumilus (3) and Bacillus sp. (19). Only six isolates (2.5%) belonged to the genus Aeribacillus represented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thermophilic bacteria; Hot springs; Diversity; 16S rRNA gene sequencing; Hydrolytic enzymes.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200443
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Xylanase production by Aspergillus awamori under solid state fermentation conditions on tomato pomace BJM
Umsza-Guez,Marcelo A.; Díaz,Ana B.; Ory,Ignacio de; Blandino,Ana; Gomes,Eleni; Caro,Ildefonso.
In this work, tomato pomace, a waste abundantly available in the Mediterranean and other temperate climates agro-food industries, has been used as raw material for the production of some hydrolytic enzymes, including xylanase, exo-polygalacturonase (exo-PG), cellulase (CMCase) and α-amylase. The principal step of the process is the solid state fermentation (SSF) of this residue by Aspergillus awamori. In several laboratory experiments, maximum xylanase and exo-PG activities were measured during the first days of culture, reaching values around 100 and 80 IU/gds (international units of enzyme activity per gram of dried solid), respectively. For CMCase and α-amylase production remained almost constant along fermentation, with average values of 19 and 21.5...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid state fermentation (SSF); Tomato pomace; Hydrolytic enzymes; Plate-type bioreactor; Xylanase production.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400046
Registros recuperados: 11
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