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| Carvalho,Cristiane S.; Melo,Edésio J.T.. |
| Toxoplasma gondii multiplies within parasitophorous vacuole that is not recognized by the primary no oxidative defense of host cells, mainly represented by the fusion with acidic organelles. Recent studies have already shown that hydroxyurea arrested the intracellular parasites leading to its destruction. In the present work we investigated the cellular mechanism involved in the destruction of intracellular Toxoplasma gondii. Fluorescent vital stains were used in order to observe possible acidification of parasitophorous vacuole-containing Toxoplasma gondii in presence of hydroxyurea. Vero cells infected with tachyzoites were treated with hydroxyurea for 12, 24 or 48 hours. Fluorescence, indicative of acidification, was observed in the parasitophorous... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hydroxyurea; Toxoplasma gondii; Parasitophorous vacuole. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300008 |
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| Melo,E.J.T.; Beiral,H.J.. |
| Toxoplasma gondii, Leishmania amazonensis and Trypanosoma cruzi are obligate intracellular parasites that multiply until lysis of host cells. The present study was undertaken to evaluate the effect of hydroxyurea (an inhibitor of cell division at the G1/S phase) on the multiplication of L. amazonensis, T. gondii, and T. cruzi in infected host cells. Infected cells were treated with hydroxyurea (4 mM) for 48 h. Hydroxyurea arrested intracellular multiplication of all infective forms of the parasites tested. In treated cultures, the percent of infected host cells decreased (50-97%) and most intracellular parasites were eliminated. Ultrastructural observations showed no morphologic change in host cells while intracellular parasites presented drastic... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Toxoplasma gondii; Trypanosoma cruzi; Leishmania amazonensis; Hydroxyurea. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100009 |
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| Silva Junior,G.B.; Libório,A.B.; Vieira,A.P.F.; Bem,A.X. Couto; Lopes Filho,A.S.; Figueiredo Filho,A.C.; Guedes,A.L.M.O.; Souza,J.H.; Costa,C.M.B.E.; Costa,R.; Daher,E.F.. |
| The objective of this study was to investigate renal function in a cohort of 98 patients with sickle cell disease (SCD) followed up at a tertiary hospital in Brazil. Clinical and laboratory characteristics at the time of the most recent medical examination were analyzed. Renal function was evaluated by the estimation of glomerular filtration rate (GFR) by the criteria of the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI). We compared patients with normal GFR to patients with decreased GFR (<60 mL·min-1·(1.73 m²)-1) and hyperfiltration (>120 mL·min-1·(1.73 m²)-1). Comparison between patients according to the use of hydroxyurea and comparison of clinical and laboratory parameters according to GFR were also carried out. Average patient... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Sickle cell disease; Kidney disease; Glomerular filtration rate; Creatinine; Chronic kidney disease; Hydroxyurea. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700013 |
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| Teixeira,R.S.; Arriaga,M.B.; Terse-Ramos,R.; Ferreira,T.A.; Machado,V.R.; Rissatto-Lago,M.R.; Silveira-Mattos,P.S.; Boa-Sorte,N.; Ladeia,A.M.T.; Andrade,B.B.. |
| Dyslipidemia has been described in sickle cell anemia (SCA) but its association with increased disease severity is unknown. Here, we examined 55 children and adolescents with SCA as well as 41 healthy controls to test the association between the lipid profiles in peripheral blood and markers of hemolysis, inflammation, endothelial function, and SCA-related clinical outcomes. SCA patients exhibited lower levels of total cholesterol (P<0.001), low-density lipoprotein cholesterol (LDL-c) (P<0.001), and high-density lipoprotein cholesterol (HDL-c) (P<0.001), while displaying higher triglyceride (TG) levels and TG/HDL-c ratio values (P<0.001). TG/HDL-c values were positively correlated with lactate dehydrogenase (P=0.047), leukocyte count (P=0.006),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sickle cell disease; Lipoproteins; Cholesterol; Triglycerides; Hydroxyurea; Endothelial function. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000612 |
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| Lima,C.S.P.; Arruda,V.R.; Costa,F.F.; Saad,S.T.O.. |
| The use of hydroxyurea (HU) can improve the clinical course of sickle cell disease. However, several features of HU treatment remain unclear, including the predictability of drug response and determination of adequate doses, considering positive responses and minimal side effects. In order to identify adequate doses of HU for treatment of sickle cell disease, 10 patients, 8 with sickle cell anemia and 2 with Sß thalassemia (8SS, 2Sß), were studied for a period of 6 to 19 months in an open label dose escalation trial (10 to 20 mg kg-1 day-1). Hemoglobin (Hb), fetal hemoglobin (Hb F) and mean corpuscular volume (MCV) values and reticulocyte, neutrophil and platelet counts were performed every two weeks during the increase of the HU dose and every 4 weeks... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sickle cell disease; Hydroxyurea; Hemoglobinopathy. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800004 |
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| Khayat,André Salim; Guimarães,Adriana Costa; Cardoso,Plínio Cerqueira; Lima,Patrícia Danielle Lima de; Bahia,Marcelo de Oliveira; Antunes,Lusânia M. Greggi; Burbano,Rommel Rodríguez. |
| Hydroxyurea is commonly used in the treatment of myeloproliferative diseases and in patients with sickle cell disease (SCD). The use of this antineoplastic agent in patients with SCD is justified because of the drug's ability to increase fetal hemoglobin levels, thereby decreasing the severity of SCD. However, high doses or prolonged treatment with hydroxyurea can be cytotoxic or genotoxic for these patients, with an increased risk of developing acute leukemia. This danger can be avoided by monitoring the lymphocytes of patients treated with hydroxyurea. Cytogenetic tests are important endpoints for monitoring the physiological effects of physical and chemical agents, including drugs. In this work, we assessed the genotoxicity of hydroxyurea in short-term... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hydroxyurea; Sickle cell disease; Mutagenesis. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100019 |
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| PELAYO,HELVIA R; PINCHEIRA,JUANA; GIMÉNEZ-ABIÁN,JUAN F; CLARKE,DUNCAN J; DE LA TORRE,CONSUELO. |
| Allium cepa L. meristems were used as a plant model to study the p53-independent control of S and G2 phases by checkpoint pathways, in eukaryotic cells. Checkpoint blocks were induced at early and mid S by hydroxyurea. After their spontaneous override, cells became accumulated in G2-prophase, giving rise later on to a delayed mitotic wave. Cell growth was maintained during the checkpoint blocks, as the delayed mitoses were larger in size than the control ones. Under continuous hydroxyurea treatment, the delayed mitotic was formed by two subpopulations: normal mitoses corresponding to cells having properly recovered from the checkpoint block, and abnormal ones resulting from checkpoint adaptation. These latter cells displayed broken chromatids as they had... |
| Tipo: Journal article |
Palavras-chave: Checkpoint adaptation; Hydroxyurea; S and G2 checkpoints; P53-independent checkpoints; Allium cepa L. meristems. |
| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300009 |
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