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| Registros recuperados: 13 | |
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| Disson, O.; Grayo, S.; Huillet, E.; Nikitas, G.; Langa-Vives, F.; Dussurget, O.; Ragon, M.; Le Monnier, A.; Babinet, C.; Cossart, P.; Lecuit, M.. |
| The ability to cross host barriers is an essential virulence determinant of invasive microbial pathogens. Listeria monocytogenes is a model microorganism that crosses human intestinal and placental barriers, and causes severe maternofetal infections by an unknown mechanism(1). Several studies have helped to characterize the bacterial invasion proteins InlA and InlB(2). However, their respective species specificity has complicated investigations on their in vivo role(3,4). Here we describe two novel and complementary animal models for human listeriosis: the gerbil, a natural host for L. monocytogenes, and a knock-in mouse line ubiquitously expressing humanized E-cadherin. Using these two models, we uncover the essential and interdependent roles... |
| Tipo: Journal Article |
Palavras-chave: LISTERIA MONOCYTOGENES; BARRIERE PLACENTAIRE; PROTEINE BACTERIENNE; VIRULENCE; AGENT PATHOGENE; MODELISATION; MICROORGANISME; INFECTION; MALADIE HUMAINE; INTESTIN. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009460b2e42&uri=/notices/prodinra1/2009/11/ |
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| Lecuit, M.; Sonnenburg, J. L.; Cossart, P.; Gordon, J. I.. |
| Members of the genus Listeria provide a model for defining host responses to invasive foodborne enteropathogens. Active translocation of Listeria monocytogenes across the gut epithelial barrier is mediated by interaction of bacterial internalin (InlA) and its species-specific host receptor, E-cadherin, whereas translocation across Peyer’s patches through M-cells is InlA independent. To define microbial determinants and molecular correlates of the host response to translocation via these two routes, we colonized germ-free transgenic mice expressing the human enterocyte-associated E-cadherin receptor with wildtype (WT) or mutant L. monocytogenes strains, or its nonpathogenic noninvasive relative Listeria innocua, or with Bacteroides thetaiotaomicron, a... |
| Tipo: Journal Article |
Palavras-chave: LISTERIA MONOCYTOGENES; CADHERINE; BARRIERE INTESTINALE; LAMINA PROPRIA; PROTEINE BACTERIENNE; INTESTIN; MODELE ANIMAL; SOURIS; INFECTION; ENTEROPATHOGENE; MUTATION; EXPRESSION DES GENES; REPONSE IMMUNITAIRE; IMMUNITE. |
| Ano: 2007 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200924d16b14&uri=/notices/prodinra1/2010/11/ |
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| Lindén, S. K.; Bierne, H.; Sabet, C.; Png, C. W.; Florin, T. H.; McGuckin, M. A.; Cossart, P.. |
| Listeria monocytogenes cross the intestinal barrier causing systemic infections with high mortality rates. Intestinal infection triggers release of intestinal mucus. We show that three L. monocytogenes internalins, InlB, InlC and InlJ all bound to MUC2 (the major component of intestinal mucus), but not to the cell surface mucin MUC1. Binding was strongest to InlB>InlC>InlJ (P < 0.001). Listerial internalins are characterized by their internalin domain, composed by leucine rich repeats (LRR) followed by an immunogloblin-like region. We report here that the internalin domain of the InlJ protein also bound MUC2, suggesting that an internalin domain is sufficient to bind to MUC2. |
| Tipo: Journal Article |
Palavras-chave: LISTERIA MONOCYTOGENES; BACTERIE; MUCINE; HOMME; INTESTIN; INFECTION; MUCUS; PROTEINE; LEUCINE; ACIDE AMINE; SURFACE CELLULAIRE; REACTION ANTIGENE ANTICORPS LISTERIA; LRRR; MUCIN; MUCUS; MUC2; MUC1. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20091b0155aa&uri=/notices/prodinra1/2010/10/ |
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| Borgonie, G.; Claeys, M.; Vanfleteren, J.; De Waele, D.; Coomans, A.. |
| Chez trois nématodes Rhabdities, #Caenorhabditis elegans, Panagrolaimus superbus$ et #Acrobeloides maximus$, les analyses ultrastructurales ont démontré la présence d'une membrane prenant naissance à l'extrémité des microvillosités intestinales, et ce sur l'entière longueur de l'intestin. Ces membranes permettent le passage de l'isothiocyanate de fluorescéine, du rouge de méthyle, du rouge neutre et de l'orange d'acridine, mais un passage très limité des molécules de ferritine. Après introduction d'une dose subléthale d'azide de sodium, la lumière intestinale est le siège d'une augmentation de la sécrétion des couches de la membrane. Des colorations #in toto$ des nématodes avec des lectines provenant de #Solanum tuberosum$ et #Triticum vulgare$, connues... |
| Tipo: Text |
Palavras-chave: NEMATODE; BACTERIOPHAGE; ULTRASTRUCTURE; MICROSCOPIE ELECTRONIQUE A TRANSMISSION; INTESTIN; COLORATION; LECTINE. |
| Ano: 1995 |
URL: http://www.documentation.ird.fr/hor/fdi:42729 |
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| Borgonie, G.; Clayes, M.; De Waele, D.; Coomans, A.. |
| L'intestin de trois nématodes libres - "Caenorhabditis elegans, panagrolaimus superbus$ et #Acrobeloides maximus$ - a été étudié en microscopie électronique à transmission sur des sections réalisées en trois localisations différentes. Entre les trois espèces, les différences ultrastructurales concernant les éléments cellulaires sont faibles encore que les deux types de cellules présents chez les trois espèces soient positionnés différemment sur le trajet de l'intestin. De plus, deux types de substances sécrétées dans la lumière intestinale ont été identifiés, l'un uniquement dans l'intestin antérieur, l'autre tout le long de l'intestin. Des colorations in toto de quinze nématodes à l'aide de la F-actine, ont révélé la présence d'un muscle intestinal bien... |
| Tipo: Text |
Palavras-chave: NEMATODE; BACTERIOPHAGE; ULTRASTRUCTURE; MICROSCOPIE ELECTRONIQUE A TRANSMISSION; INTESTIN; COLORATION. |
| Ano: 1995 |
URL: http://www.documentation.ird.fr/hor/fdi:42717 |
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| Registros recuperados: 13 | |
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