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| Dutech, C.; Enjalbert, J.; Fournier, E.; Delmotte, F.; Barrès, B.; Carlier, J.; Tharreau, D.; Giraud, T.. |
| Although they represent powerful genetic markers in many fields of biology, microsatellites have been isolated in few fungal species. The aim of this study was to assess whether obtaining microsatellite markers with an acceptable level of polymorphism is generally harder from fungi than in other organisms. We therefore surveyed the number,nature and polymorphism level of published microsatellite markers in fungi from the literature and from our own data on seventeen fungal microsatellite-enriched libraries, and in five other phylogroups (angiosperms, insects, fishes, birds and mammals). Fungal microsatellites indeed appeared both harder to isolate and to exhibit lower polymorphism than in other organisms. This appeared to be due, at least in part, to... |
| Tipo: Journal Article |
Palavras-chave: MARQUEUR GENETIQUE; MICROSATELLITE; CHAMPIGNON; ANALYSE DE DONNEES SSR; PLANT PATHOGEN; POLYMORPHIC; MARKERS; DEVELOPMENT; ISOLATION; FEATURES; AFFECTING POLYMORPHISM. |
| Ano: 2007 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200780b37b09&uri=/notices/prodinra1/2008/04/ |
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| Monti, L.; Lalanne-Cassou, B.; Lucas, P.; Malosse, C.; Silvain, J.F.. |
| S. latifascia and S. descoinsi are closely related species that occur sympatrically over limited areas in French Guiana. We examined allopatric populations, S. latifascia originating from Barbados and S. descoinsi from French Guiana. Studies on nocturnal activity cycles showed temporal partitioning of female calling behavior, male sexual activity, and mating behavior.S. descoinsi were sexually active in the first half of the scotophase whereas S. latifascia were sexually active in the second half. Seven compounds (Z9–14: Ac,Z9,E12–14: Ac,Z11–16: Ac,E9,E12–14: Ac,Z9–14: Ald,Z9,E11–14: Ac andZ11–14: Ac) were identified in females of both S. latifascia and S. descoinsi extracts.Z9–14: Ac was a main pheromone component for the two species. The major difference... |
| Tipo: Journal Article |
Palavras-chave: SPODOPTERA; LEPIDOPTERE; NOCTUIDAE; ACTIVITE SEXUELLE; ATTRACTION SEXUELLE; ISOLATION; PHEROMONE SEXUELLE; RYTHME D'ACTIVITE; ELECTROPHYSIOLOGIE; REPRODUCTION LEPIDOPTERA; NOCTUIDAE; SPODOPTERA LATIFASCIA; SPODOPTERA DESCOINSI; PHEROMONES; (Z)-9-TETRADECENYL ACETATE; (Z E)-9 12-TETRADECADIENYL ACETATE; SEXUAL ACTIVITY RHYTHMS; CROSS-ATTRACTION; ELECTROPHYSIOLOGY; REPRODUCTIVE ISOLATION. |
| Ano: 1995 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9600002138054208&uri=/notices/prodinra1/2010/11/ |
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| Appeldoorn, M.M.; Sanders, M.; Vincken, J.P.; Cheynier, V.; Le Guerneve, C.; Hollman, P.C.H.; Gruppen, H.. |
| In order to fully explore the biofunctional potential of proanthocyanidins (PA), isolated and well-characterised PA dimers are of great importance. Current methods to obtain pure A- and B-type dimers are laborious, because they comprise multiple chromatographic steps, often yielding only one or two specific dimers. In the current study, an efficient isolation procedure is described, to isolate a large variety of A-type dimers from peanut skins and B-type dimers from grape seeds. Yields increased 20-400 times for A-type dimers and about 10 times for B-type dimers compared to other methods with a lesser number of chromatographic steps. Dinners isolated from peanut skins were identified as; epicatechin-(2-O-7, 4-8)-catechin (A1), epicatechin-(2-O-7,... |
| Tipo: Journal Article |
Palavras-chave: GRAPE SEED; PEANUT SKIN; ISOLATION; PROCYANIDIN DIMER; REVERSED-PHASE HPLC. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20094344e39b&uri=/notices/prodinra1/2010/08/ |
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| Barbier, M.; Reichstein, T.; Schindler, O.; Lederer, E.. |
| IN connexion with an attempt to isolate pheromone from the queens of the honey bee, Apis mellifica L., an examination was carried out on the neutal portion of the extract obtained by perfusion of the powdered whole-bodies of the queens with ethanol and tert-butanol. By chromatographing the neutral portion on alumina, or silica gel, a crystalline substance, m.p. 138–145° C. [α]D = −31.6 ± 6° (c = 0.39 in chloroform), was obtained, which resembled cholesterol in behaviour and chemical properties. The Liebermann-Burchard reaction gave the same coloration as that obtained with cholesterol and the mixed melting point was the same. However, there were two bands (6.08µ and 11.33µ) in the infra-red spectrum of the substance which are not found in the spectrum of... |
| Tipo: Journal Article-postprint |
Palavras-chave: HONEYBEE; APIS MELLIFERA; APIDAE; HYMENOPTERA; SOCIAL INSECT; QUEEN; 24-METHYLENE-CHOLESTEROL; ISOLATION; ABEILLE DOMESTIQUE; INSECTE SOCIAL; REINE; 24-METHYLENE-CHOLESTEROL; ISOLATION. |
| Ano: 1959 |
URL: http://hdl.handle.net/2174/504 |
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| Bailey, L.. |
| An account is given of the development of a reliable method for the isolation of Streptococcus pluton (Bacillus pluton White), an organism associated with European foul-brood disease of the larval honeybee. S. pluton, isolated as an anaerobe, may be trained to grow as an aerobe in rod form. Its principal anaerobic growth requirements are a low molar ratio of Na: K, high inorganic phosphate concentration, glucose or fructose, and undetermined factors provided by yeast extract. Peptones are harmful to growth. Aerobic growth has no very critical requirements other than glucose, fructose or sucrose. Bacterium eurydice White which, together with S. pluton, causes European foul-brood disease grows well anaerobically on a yeast extract + glucose + fructose... |
| Tipo: Journal Article-postprint |
Palavras-chave: HONEYBEE; APIS MELLIFERA; APIDAE; HYMENOPTERA; SOCIAL INSECT; LARVAE; EUROPEAN FOULBROOD; INFECTIOUS DISEASE; STREPTOCOCCUS PLUTON; BACTERIUM EURYDICE; ISOLATION; CULTURE; ABEILLE DOMESTIQUE; INSECTE SOCIAL; LARVE; LOQUE EUROPEENNE; MALADIE INFECTIEUSE; STREPTOCOCCUS PLUTON; BACTERIUM EURYDICE; ISOLATION; CULTURE. |
| Ano: 1957 |
URL: http://hdl.handle.net/2174/500 |
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| Garret, A.; Kerlan, C.; Thomas, D.. |
| The chronology of PLRV acquisition and retention byMyzus persicae was investigated using electron microscopy. Examination demonstrated a rapid translocation of the virus through the intestine into the haemocoel. Indeed, viral particles could be observed in the intestinal epithelial cells, then in the haemocoel, 4 and 8 h, respectively, after their arrival in the lumen of the alimentary canal. However, the virus accumulated in the intestinal epithelial cells. In these cells, the first viral particles were seen enclosed in isometric or tubular isolated vesicles; a few hours later, they were present in tubular aggregated vesicles and also in lysosomes or multivesicular bodies. After a 40 h acquisition period, all studied intestinal epithelial cells exhibited... |
| Tipo: Journal Article |
Palavras-chave: PUCERON; MYZUS PERSICAE; LUTEOVIRUS; ENROULEMENT DE LA POMME DE TERRE; VECTEUR DE MALADIE; ISOLATION; PURIFICATION; TEST ELISA; ULTRASTRUCTURE. |
| Ano: 1996 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9900012957074901&uri=/notices/prodinra1/2010/11/ |
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