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45-and 70-base DNA supramolecular polymerizations on quartz crystal microbalance biosensor ArchiMer
Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Antoine, Elisabeth; Compere, Chantal.
Supramolecular polymerizations of 45- and 70- base DNAs on the surface of an in-situ time-resolved 27 MHz quartz crystal microbalance biosensor.
Tipo: Text Palavras-chave: Sensor; Design; Kinetics; Immobilization; Hybridization.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1710.pdf
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A comparative study on fungal laccases immobilized on chitosan BABT
Araújo,José Hilton Bernardino de; Uemura,Vinicius Oliveira; Moraes,Flavio Faria de; Barbosa,Aneli de Melo; Zanin,Gisella Maria.
The phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laccases; Immobilization; Chitosan.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001
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"A comparison between sugar consumption and ethanol production in wort by immobilized Saccharomyces Cerevisiae, Saccharomyces Ludwigii and Saccharomyces Rouxii on Brewer's Spent Grain" BJM
Mohammadi,Aniseh; Razavi,Seyyed Hadi; Mousavi,Seyyed Mohammad; Rezaei,Karamatollah.
The immobilization of Saccharomyces cerevisiae DSM 70424, Saccharomyces ludwigii DSM 3447 and Saccharomyces rouxii DSM 2531 on brewer's spent grain and then ethanol production and sugar consumption of these immobilized yeasts were investigated. The aim of this study was to investigate the abilities of these three immobilized yeasts for producing alcohol for brewing at two temperatures (7 and 12 °C) using two different sugar levels (one at original level supplied in the brewery and one with 2.5% (w/v), added glucose to the wort). Increasing both parameters resulted in higher alcohol production by all the yeasts studied. At 7 °C and with original wort density the ethanol content at the end of fermentation was 2.7% (v/v) for S. cerevisiae, 1.7% for S....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brewer's spent grain; Fermentation; Immobilization; Saccharomyces cerevisiae; Saccharomyces ludwigii; Saccharomyces rouxii.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200025
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Adsorption, immobilization and activity of cellulase in soil: the impacts of maize straw and its humification BABT
Sinegani,Ali Akbar Safari; Sinegani,Mahboobe Safari.
The present work aimed to study some aspects of sorption and immobilization of cellulase molecules on soil components by the analysis of the reactions of cellulase in a soil treated with different levels of maize residue and incubated for 90 days. The analysis of variance showed that the effects of the treatments of maize straw, incubation time and their interaction on cellulase adsorption, desorption and immobilization were statistically significant. The adsorption and immobilization capacities of soil by application of maize straw increased significantly. However they decreased with decreasing the soil organic matter (SOM) after 45 days of incubation. The desorption of adsorbed cellulase molecules from the soil by washing with distilled water depended on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Maize straw; Humification; Cellulase; Sorption; Immobilization; Activity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600001
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Advances in enzyme bioelectrochemistry Anais da ABC (AABC)
PEREIRA,ANDRESSA R.; SEDENHO,GRAZIELA C.; SOUZA,JOÃO C. P. DE; CRESPILHO,FRANK N..
ABSTRACT Bioelectrochemistry can be defined as a branch of Chemical Science concerned with electron-proton transfer and transport involving biomolecules, as well as electrode reactions of redox enzymes. The bioelectrochemical reactions and system have direct impact in biotechnological development, in medical devices designing, in the behavior of DNA-protein complexes, in green-energy and bioenergy concepts, and make it possible an understanding of metabolism of all living organisms (e.g. humans) where biomolecules are integral to health and proper functioning. In the last years, many researchers have dedicated itself to study different redox enzymes by using electrochemistry, aiming to understand their mechanisms and to develop promising bioanodes and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioelectrocatalysis; Biofuel cells; Bioelectrochemistry; Immobilization; Protein-electrode interactions; Redox enzymes.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000200825
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Amplitude no fósforo microbiano em um Argissolo em pastagem nativa submetida à roçada e à introdução de espécies forrageiras com fertilização fosfatada em diferentes épocas - DOI: 10.4025/actasciagron.v30i4.5319 Agronomy
Santos, Danilo Rheinheimer; UFSM; Martinazzo, Rosane; UFRGS; Gatiboni, Luciano Colpo; UDESC; Kaminski, João; UFSM; Silva, Leandro Souza da; UFSM.
Em solos com elevado grau de intemperismo, a imobilização temporária de fósforo pela biomassa microbiana do solo (BMS) pode ser um mecanismo de retardamento das reações de adsorção. O objetivo do presente trabalho foi avaliar a amplitude no fósforo microbiano (Pm) em um Argissolo em pastagem nativa submetida à roçada, introdução de forrageiras exóticas e adubadas com fertilizantes fosfatados. O estudo foi desenvolvido a partir de um experimento instalado em 1997 que constava de cinco tratamentos de fosfato e calagem (testemunha sem fósforo; hiperfosfato de gafsa; superfosfato triplo; superfosfato triplo + calcário e superfosfato simples + calcário), distribuídos em quatro blocos ao acaso. Em junho de 2002, a pastagem nativa remanescente foi roçada, semeada...
Palavras-chave: 5.01.00.00-9 Agronomia biomassa microbiana; Imobilização; Adubos fosfatados; Pastagens nativas; Disponibilidade de fósforo. microbial biomass; Immobilization; Phosphate fertilizers; Natural pasture; Phosphorus availability.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5319
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Analysis of fracture healing in osteopenic bone caused by disuse: experimental study BJMBR
Paiva,A.G.; Yanagihara,G.R.; Macedo,A.P.; Ramos,J.; Issa,J.P.M.; Shimano,A.C..
Osteoporosis has become a serious global public health issue. Hence, osteoporotic fracture healing has been investigated in several previous studies because there is still controversy over the effect osteoporosis has on the healing process. The current study aimed to analyze two different periods of bone healing in normal and osteopenic rats. Sixty, 7-week-old female Wistar rats were randomly divided into four groups: unrestricted and immobilized for 2 weeks after osteotomy (OU2), suspended and immobilized for 2 weeks after osteotomy (OS2), unrestricted and immobilized for 6 weeks after osteotomy (OU6), and suspended and immobilized for 6 weeks after osteotomy (OS6). Osteotomy was performed in the middle third of the right tibia 21 days after tail...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Osteoporosis; Osteotomy; Fracture healing; Immobilization; Rats.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300708
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Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843 Electron. J. Biotechnol.
Riansa-ngawong,Wiboon; Suwansaard,Maneewan; Prasertsan,Poonsuk.
Palm pressed fiber (PPF) is a clean and renewable lignocellulosic material. The PPF and delignified PPF (DPPF) were used as a carrier for immobilization of Candida shehatae TISTR5843 in bioethanol production. PPF was pre-treated by milling to obtain small particles, whereas DPPF was the delignification of PPF using NaClO2. C. shehatae TISTR5843 was grown in modified yeast extract- malt (YM) medium at 30 ± 2ºC on an orbital shaker at 150 rpm for batch and repeated batch fermentation. In the batch system, immobilized cells on a small size, less than 0.5 mm, of DPPF (sDPPF) gave the maximum ethanol production of 11.5 g L-1 at 24 hrs cultivation period. The ethanol concentration and ethanol yield of sDPPF were 6.2% and 6.8% higher (ethanol production 11.5 g...
Tipo: Journal article Palavras-chave: Candida shehatae; Ethanol production; Immobilization; Palm pressed fiber.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600001
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Biodegradation of acetonitrile by cells of Candida guilliermondii UFMG-Y65 immobilized in alginate, kappa-carrageenan and citric pectin BJM
Dias,João Carlos T.; Rezende,Rachel P.; Linardi,Valter R..
Different encapsulation matrices were tested for immobilized cells of Candida guilliermondii UFMG-Y65 used for acetonitrile degradation. Acetonitrile degradation by free cells and cells immobilized in Ba-alginate, kappa-carrageenan and citric pectin was studied. The rate of acetonitrile degradation was monitored for 120 h by measuring yeast growth and ammonia concentration. Different alginate concentrations did not affect cell viability, but the period of incubation in BaCl2 solution reduced the number of viable cells. Likewise, the gel nature and the matrix structure of the support resulting from the cell immobilization conditions were of fundamental importance for biocatalyst activity and performance, affecting substantially the patterns of microbial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida guilliermondii; Acetonitrile; Biodegradation; Immobilization.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100015
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Biodegradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1 BJM
Tallur,Preeti N.; Mulla,Sikandar I.; Megadi,Veena B.; Talwar,Manjunatha P.; Ninnekar,Harichandra Z..
Pyrethroid pesticide cypermethrin is a environmental pollutant because of its widespread use, toxicity and persistence. Biodegradation of such chemicals by microorganisms may provide an cost-effective method for their detoxification. We have investigated the degradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1 in various matrices such as, polyurethane foam (PUF), polyacrylamide, sodium alginate and agar. The optimum temperature and pH for the degradation of cypermethrin by immobilized cells of Micrococcus sp. were found to be 30 °C and 7.0, respectively. The rate of degradation of 10 and 20 mM of cypermethrin by freely suspended cells were compared with that of immobilized cells in batches and semi-continuous with shaken...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Degradation; Immobilization; Cypermethrin; Polyurethane foam; Micrococcus sp. strain CPN 1.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300667
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Biosensor for determination of glucose in real samples of beverages Ciênc. Tecnol. Aliment.
Lopes,Flavio Marques; Batista,Karla de Aleluia; Batista,Gustavo Luiz Aleluia; Fernandes,Kátia Flávia.
A biosensor was developed for spectrophotometric determination of glucose concentrations in real samples of orange juice energetic drinks, and sport drinks. The biosensor consisted of glucose oxidase (GOD) and horseradish peroxidase (HRP) immobilized onto polyaniline activated with glutaraldehyde (PANIG). Immobilization parameters were optimized for GOD, and maximum immobilization yield was 16% when 5.0 mg of PANIG and 8.9 U prepared in 0.1 mol.L-1 sodium phosphate buffer (pH 7.0) reacted for 60 minutes at 4 °C with gentle stirring. The linear operational range for glucose determination using optimized operational parameters was between 0.05 and 6.0 mg.mL-1 with a very good reproducibility of response. The results obtained in the biosensor were compared...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosensor; Glucose; Glucose oxidase; Immobilization; Polyaniline.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100010
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Characteristics of Immobilized Urease on Grafted Alginate Bead Systems BABT
Danial,Enas N.; Hamza,Amal H.; Mahmoud,Rasha H..
This study evaluated the biological importance of immobilized urease enzyme over the free urease. The support material used for urease immobilization was alginate. Generally, the immobilization of urease in alginate gel showed a marked increase in Km and Vmax. However, the immobilized urease showed higher thermal stability than that of free enzyme. The rate of thermal inactivation of the immobilized enzyme decreased due to entrapment in gel matrix. Also, the activity of the immobilized urease was more stable in retention than that of the free enzyme during the storage in solution, although the activity of the immobilized enzyme was lower in comparison with the free enzyme. A stable immobilized system and long storage life are convenient for applications...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Urease; Immobilization; Kinetic; Reusability; Storage stability.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200147
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Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles BABT
Bruno,Laura Maria; Lima Filho,José Luiz de; Castro,Heizir Ferreira de.
Microbial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activities were found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida lipase; Mucor lipase; Hybrid matrix; Immobilization.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500003
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Continuous ethanol production using immobilized yeast cells entrapped in loofa-reinforced alginate carriers BJM
Bangrak,Phoowit; Limtong,Savitree; Phisalaphong,Muenduen.
A culture of Saccharomyces cerevisiae M30 entrapped in loofa-reinforced alginate was used for continuous ethanol fermentation in a packed-bed reactor with initial sugar concentrations of 200-248 g/L. Maximum ethanol productivity of 11.5 g/(L·h) was obtained at an ethanol concentration of 57.4 g/L, an initial sugar concentration of 220 g/L and a dilution rate (D) of 0.2 h-1. However, a maximum ethanol concentration of 82.1 g/L (productivity of 9.0 g/(L·h)) was obtained at a D of 0.11 h-1. Ethanol productivity in the continuous culture was 6-8-fold higher than that in the batch culture. Due to the developed carrier's high biocompatibility, high porosity, and good mechanical strength, advantages such as cell regeneration, reusability, altered mechanical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethanol; Loofa; Alginate; Immobilization; Continuous.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200032
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Cultivation of an immobilized (hyper)thermophilic marine microbial community in a bioreactor ArchiMer
Landreau, M.; Duthoit, Frederique; Roussel, Erwan; Schonherr, S.; Georges, Myriam; Godfroy, Anne; Le Blay, G..
Cultivation in a bioreactor of immobilized deep-sea hydrothermal microbial community was tested in order to assess the stability and reactivity of this new system. A community composed of 8 hydrothermal strains was entrapped in a polymer matrix that was used to inoculate a continuous culture in a gas-lift bioreactor. The continuous culture was performed for 41 days at successively 60°C, 55°C, 60°C, 85°C and 60°C, at pH 6.5, in anaerobic condition and constant dilution rate. Oxic stress and pH variations were tested at the beginning of the incubation. Despite these detrimental conditions, 3 strains including 2 strict anaerobes were maintained in the bioreactor. High cell concentrations (3 × 108 cells mL−1) and high ATP contents were measured in both liquid...
Tipo: Text Palavras-chave: Immobilization; Anaerobiosis; Marine (hyper)thermophiles; Continuous culture.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00347/45851/45516.pdf
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Cyclodextrin glucanotransferase immobilization onto functionalized magnetic double mesoporous core-shell silica nanospheres Electron. J. Biotechnol.
Ibrahim,Abdelnasser S.S.; Al-Salamah,Ali A; El-Toni,Ahmed Mohamed; El-Tayeb,Mohamed A; Elbadawi,Yahya B.
Background Cyclodextrin glucanotransferase (CGTase) from Amphibacillus sp. NPST-10 was covalently immobilized onto amino-functionalized magnetic double mesoporous core-shell silica nanospheres (mag@d-SiO2@m-SiO2-NH2), and the properties of the immobilized enzyme were investigated. The synthesis process of the nanospheres included preparing core magnetic magnetite (Fe3O4) nanoparticles, coating the Fe3O4 with a dense silica layer, followed by further coating with functionalized or non-functionalized mesoporous silica shell. The structure of the synthesized nanospheres was characterized using TEM, XRD, and FT-IR analyses. CGTase was immobilized onto the functionalized and non-functionalized nanospheres by covalent attachment and physical adsorption. Results...
Tipo: Journal article Palavras-chave: Amphibacillus sp; Cyclodextrin glucanotransferase; Cyclodextrins; Double mesoporous core-shell silica nanospheres; Immobilization.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200001
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Cyclosporin-A does not affect skeletal muscle mass during disuse and recovery BJMBR
Aoki,M.S.; Miyabara,E.H.; Soares,A.G.; Salvini,T.F.; Moriscot,A.S..
Cyclosporin-A (CsA) is an immunosuppressive drug that acts as an inhibitor of calcineurin, a calcium phosphatase that has been suggested to play a role in skeletal muscle hypertrophy. The aim of the present study was to determine the effect of CsA administration (25 mg kg-1 day-1) on skeletal muscle mass and phenotype during disuse and recovery. Male Wistar rats received vehicle (N = 8) or CsA (N = 8) during hind limb immobilization (N = 8) and recovery (N = 8). Muscle weight (dry/wet) and cross-sectional area were evaluated to verify the effect of CsA treatment on muscle mass. Muscle phenotype was assessed by histochemistry of myosin ATPase. CsA administration during immobilization and recovery did not change muscle/body weight ratio in the soleus (SOL)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calcineurin; Immobilization; Recovery; Skeletal muscle; Hypertrophy; Cyclosporin-A.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200011
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Decreased spermatogenic and androgenic testicular functions in adult rats submitted to immobilization-induced stress from prepuberty BJMBR
Almeida,S.A.; Petenusci,S.O.; Anselmo-Franci,J.A.; Rosa-e-Silva,A.A.M.; Lamano-Carvalho,T.L..
We investigated whether chronic stress applied from prepuberty to full sexual maturity interferes with spermatogenic and androgenic testicular functions. Male Wistar rats (40 days old) were immobilized 6 h a day for 60 days. Following immobilization, plasma concentrations of corticosterone and prolactin increased 135% and 48%, respectively, while plasma luteinizing hormone and testosterone presented a significant decrease of 29% and 37%, respectively. Plasma concentration of follicle-stimulating hormone was not altered in stressed rats. Chronic stress reduced the amount of mature spermatids in the testis by 16% and the spermatozoon concentration in the cauda epididymidis by 32%. A 17% reduction in weight and a 42% decrease in DNA content were observed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Spermatogenesis; LH; FSH; Testosterone; Prolactin.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100013
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Effect of one stretch a week applied to the immobilized soleus muscle on rat muscle fiber morphology BJMBR
Gomes,A.R.S.; Coutinho,E.L.; França,C.N.; Polonio,J.; Salvini,T.F..
We determined the effect of stretching applied once a week to the soleus muscle immobilized in the shortened position on muscle fiber morphology. Twenty-six male Wistar rats weighing 269 ± 26 g were divided into three groups. Group I, the left soleus was immobilized in the shortened position for 3 weeks; group II, the soleus was immobilized in the shortened position and stretched once a week for 3 weeks; group III, the soleus was submitted only to stretching once a week for 3 weeks. The medial part of the soleus muscle was frozen for histology and muscle fiber area evaluation and the lateral part was used for the determination of number and length of serial sarcomeres. Soleus muscle submitted only to immobilization showed a reduction in weight (44 ± 6%, P...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Muscle; Stretching; Immobilization; Sarcomere number; Muscle fiber area.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000005
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Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge BABT
Marques,Leila Larisa Medeiros; Buzato,João Batista; Celligoi,Maria Antonia Pedrine Colabone.
This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Invertase; Luffa cylindrica; Raffinose; Saccharomyces cerevisiae; Ultrasound.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700003
Registros recuperados: 57
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