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Registros recuperados: 27
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A 19-year old man with IgA vasculitis after vaccination BJID
Malek,Alexandre; Gomez-Villegas,Sara Isabel; de la Hoz,Alejandro; Nowbakht,Cima; Arias,Cesar A..
ABSTRACT A 19-year-old patient who mistakenly received two doses of influenza vaccine 10 days before presentation, was admitted with malaise, weakness, and a purpuric non-blanching rash most prominent on the ankles followed by abdominal pain and hematochezia 72 h later. The diagnosis of influenza vaccine-related Henoch–Schonlein vasculitis was made. This complication, although rare, is the most common vasculitis related to immunization.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Influenza vaccination; Immunization; IgA vasculitis; Henoch–Schonlein purpura.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000500442
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A glycoprotein E gene-deleted bovine herpesvirus 1 as a candidate vaccine strain BJMBR
Weiss,M.; Brum,M.C.S.; Anziliero,D.; Weiblen,R.; Flores,E.F..
A bovine herpesvirus 1 (BoHV-1) defective in glycoprotein E (gE) was constructed from a Brazilian genital BoHV-1 isolate, by replacing the full gE coding region with the green fluorescent protein (GFP) gene for selection. Upon co-transfection of MDBK cells with genomic viral DNA plus the GFP-bearing gE-deletion plasmid, three fluorescent recombinant clones were obtained out of approximately 5000 viral plaques. Deletion of the gE gene and the presence of the GFP marker in the genome of recombinant viruses were confirmed by PCR. Despite forming smaller plaques, the BoHV-1△gE recombinants replicated in MDBK cells with similar kinetics and to similar titers to that of the parental virus (SV56/90), demonstrating that the gE deletion had no deleterious effects...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle pathogen; Immunization; Control; Differential vaccine.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900843
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Active Immunization Against 5α-androstane-3α, 17β-diol Increases Ovulation Rate in Merino Ewes OAK
TETSUKA, Masafumi; NANCARROW, Colin. D.; 手塚, 雅文.
Cyclic Merino ewes were immunized three times against 5α−androstane-3α,17β-diol (3α-diol) at three week intervals (weeks 0,3 and 6). After the third immunization, estrous cycles were synchronized by a series of PG injections and intensive blood samples were obtained for gonadotropin analyses during the mid-luteal phase and during the early follicular phase. Ovulation rates were determined after each immunization and subsequent cloprostenol (PG)-induced luteolysis (weeks 3, 6, 8 and 11). After the second immunization, daily blood samples were obtained and concentrations of plasma progesterone (P) were measured. Immunization against 3α-diol significantly increased the ovulation rate after the second and third immunizations and at week 10 (P<0.01,...
Palavras-chave: Ovulation rate; Sheep; Immunization; 5a-reduced androgen.
Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3033
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Adjuvant Effect of Oligomannose-Coated Liposome-Based Platform for veccine against African Trypanosomosis OAK
Kuboki, Noritaka; Yokoyama, Naoaki; Namangala, B; Okamura, Masashi; Inoue, Noboru; Takagi, Hideaki; Nakayama, Tomoko; Nishikawa, Yoshifumi; Ikehara, Yuzuru; Kojima, Naoya; 横山, 直明; 井上, 昇; 西川, 義文.
Palavras-chave: Adjuvant; African trypanosome; Immunization; Oligomannose-coated liposome(OML).
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2222
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Alternatives for methane emission mitigation in livestock systems R. Bras. Zootec.
Lascano,Carlos E.; Cárdenas,Edgar.
Human activities are contributing to Global Climate Change through the production of Green House Gases (GHG), which result in increased air, land and ocean temperatures and extreme changes in precipitation in regions of low and high rainfall. The most important GHG's are carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O). It is estimated that 18 % of the annual GHG emissions come from different types of livestock and that 37% of CH4, with higher global warming potential (23) relative to CO2 (1), comes from fermentation processes in ruminants. It is possible that in the future beef and milk exports from producing countries is subject to bans if cattle systems do not comply with measures to reduce GHG. There are several alternatives available and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Climate change; Defaunation; Feed chemical additives; Genetic transformation of bacteria; Greenhouse gases; Immunization; Saponins; Tannins.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300020
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Assessment of the acid phosphatase CP01850 from Corynebacterium pseudotuberculosis in DNA and subunit vaccine formulations against caseous lymphadenitis Arq. Bras. Med. Vet. Zootec.
Rezende,A.F.S.; Brum,A.A.; Bezerra,F.S.B.; Braite,D.C.; Sá,G.L.; Thurow,H.S.; Seixas,F.K.; Azevedo,V.A.C.; Portela,R.W.; Borsuk,S..
ABSTRACT The target cp1002_RS01850 from Corynebacterium pseudotuberculosis was used to construct a DNA and recombinant subunit vaccine against caseous lymphadenitis. Recombinant protein rCP01850 was expressed in Escherichia coli using pAE vector, and DNA vaccine was engineered with pTARGET vector. BALB/c mice were divided in five groups containing eight animals each, inoculated with: pTARGET/cp01850 as DNA vaccine (G1); rCP01850 plus Al (OH)3 as recombinant subunit vaccine (G2); pTARGET/cp01850 and a boost with rCP01850 plus Al (OH)3 (G3); pTARGET (G4); or Al (OH)3 (G5). Mice were inoculated and blood samples were collected on days 0, 21, and 42 for the analysis of total IgG, IgG1 and IgG2a by ELISA. In each group, five animals were challenged with Mic-6...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cp1002_RS01850 gene; Immunization; Recombinant protein; Aluminum hydroxide; Heterologous prime-boost strategy.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100199
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Assessment of the plan for pre-exposition vaccination with Fuenzalida-Palacios anti-rabies vaccine BABT
Preto,Angela Aparecida; Gomes,Elisa Maines; Fernandes,Maurício José; Hennig,Nei Adolfo; Germano,Pedro Manoel Leal.
Studies ere carried out to monitor the titres of anti-rabies antibodies taken from individuals exposed to the risk of infection. Fifty-seven individuals aged between 20 and 40, were vaccinated with a minimum titre of 1.3 IU Fuenzalida-Palacios type anti-rabies vaccine. The vaccination plan consisted of the application of three doses at two day intervals and a booster applied 28 days after the final dose. The accompainment of the antibody titres was achieved by means of the seroneutralization test over a total period of 18 months. Through the monitoring carried out, it was found to be necessary to apply a booster vaccination to part of the group. Some of these individuals were revaccinated with Fuenzalida-Palacios type vaccine with a minimum titre of 1.3...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunization; Rabies vaccine; Antibodies; Neutralization; Fuenzalida-Palacios.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300002
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Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors Anais da ABC (AABC)
Ferreira,Luís C.S.; Ferreira,Rita C.C.; Schumann,Wolfgang.
Bacillus subtilis and some of its close relatives have a long history of industrial and biotechnological applications. Search for antigen expression systems based on recombinant B. subtilis strains sounds attractive both by the extensive genetic knowledge and the lack of an outer membrane, which simplify the secretion and purification of heterologous proteins. More recently, genetically modified B. subtilis spores have been described as indestructible delivery vehicles for vaccine antigens. Nonetheless both production and delivery of antigens by B. subtilis strains face some inherent obstacles, as unstable gene expression and reduced immunogenicity that, otherwise, can be overcome by already available gene technology approaches. In the present review we...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vaccines; Bacillus subtilis; Immunization; Protein expression.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000100009
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Coping with genetic diversity: the contribution of pathogen and human genomics to modern vaccinology BJMBR
Lemaire,D.; Barbosa,T.; Rihet,P..
Vaccine development faces major difficulties partly because of genetic variation in both infectious organisms and humans. This causes antigenic variation in infectious agents and a high interindividual variability in the human response to the vaccine. The exponential growth of genome sequence information has induced a shift from conventional culture-based to genome-based vaccinology, and allows the tackling of challenges in vaccine development due to pathogen genetic variability. Additionally, recent advances in immunogenetics and genomics should help in the understanding of the influence of genetic factors on the interindividual and interpopulation variations in immune responses to vaccines, and could be useful for developing new vaccine strategies....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human vaccines; Vaccinomics; Genetic variation; Immunization.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500001
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Destruction of immunobiologicals and their disposal J. Venom. Anim. Toxins incl. Trop. Dis.
Gatti,MAN; Cardoso,SNM; Simeão,SFAP; Vitta,A; Monteiro,LM.
The guidelines of the Brazilian National Health System (SUS) state that the municipal authorities are responsible for the vaccination of the population. The present study examined the types of immunobiologicals, amounts and reasons for their destruction and disposal by the Municipal Health Secretariat in the city of Bauru, São Paulo state, Brazil, between 2008 and 2009. This study comprises a descriptive, exploratory and retrospective work that employed search of bibliographic data, collection of secondary data from forms of immunobiologicals disposal and interview of the agent responsible for the disposal of these products in the municipality. It was observed that the total numbers of unused vaccines in 2008 and 2009 were similar (4523 and 4395,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunization; Biologic waste disposal; Health services.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400017
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Diagnostics of tuberculosis and differentiation of nonspecific tuberculin reactions in animals BJM
Basybekov,Sovetzhan Z.; Bazarbayev,Marat B.; Yespembetov,Bolat A.; Mussaeva,Assiya; Kanatbayev,Serik G.; Romashev,Kanapya M.; Dossanova,Aigul K.; Yelekeyev,Tokseiit A.; Akmatova,Elmira K.; Syrym,Nazym S..
Abstract Tuberculosis is a serious disease of humans and animals, caused by bacteria of the Mycobacterium genus. This leads to complications in the life of the sick person, and subsequently to death. The cattle, who have been diagnosed with this bacterium, are usually sent to the slaughter, with the result that their livestock is reduced. Mycobacteriosis is also a disease, after determining which cattle are most often sent to slaughter. Such a reduction in livestock numbers has a negative effect on the economy. Of the 300 samples from the animals, 25 cultures of atypical bacteria responding to tuberculin were isolated. A series of tests - intravenous tuberculin test, ophthalmic test, palpebral test, "ZhAT" test, showed that most of the tuberculosis changes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Atypical mycobacterium; Tuberculin skin test; Palpebral method; Immunization.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200329
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Effect of a single tetanus-diphtheria vaccine dose on the immunity of elderly people in São Paulo, Brazil BJMBR
Weckx,L.Y.; Divino-Goes,K.; Lihama,D.M.; Carraro,E.; Bellei,N.; Granato,C.F.H.; de Moraes-Pinto,M.I..
Epidemiological data regarding tetanus and diphtheria immunity in elderly people in Brazil are scarce. During the First National Immunization Campaign for the Elderly in Brazil in April 1999, 98 individuals (median age: 84 years) received one tetanus-dyphtheria (Td) vaccine dose (Butantan Institute, lot number 9808079/G). Inclusion criteria were elderly individuals without a history of severe immunosuppressive disease, acute infectious disease or use of immunomodulators. Blood samples were collected immediately before the vaccine and 30 days later. Serum was separated and stored at -20ºC until analysis. Tetanus and diphtheria antibodies were measured by the double-antigen ELISA test. Tetanus and diphtheria antibody concentrations lower than 0.01 IU/mL were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Elderly; Tetanus; Diphtheria; Immunization.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400012
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Efficiency of inactive vaccines against contagious agalactia in Brazil Arq. Bras. Med. Vet. Zootec.
Campos,A.C.; Azevedo,E.O.; Alcântara,M.D.B.; Silva,R.B.S.; Cordeiro,A.A.; Mamede,A.G.; Melo,M.A.; Rosendo Nascimento,E.; Castro,R.S..
This paper aims to evaluate the efficiency of three inactive vaccines against contagious agalactia prepared with samples of Mycoplasma agalactiae isolated in Brazil and different adjuvants. Vaccine 1 adsorbed with aluminum hydroxide was administered in 23 goats (Gc1) and 13 sheep (Gov1); vaccine 2 containing Montanide IMS-2215-VG was administered in 22 goats (Gc2) and 12 sheep (Gov2) and vaccine 3, containing Montanide Gel-01 was administered in 22 goats (Gc3) and 12 sheep (Gov3). All animals were negative for Ma at indirect ELISA and received two doses of 2mL each, subcutaneously, within a 21 day interval. Five animals from each species were used as control. Seventy-five days after the booster, four animals from each vaccinated group and two from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma agalactiae; Goats; Sheep; Immunization; ELISA.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500018
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Enhanced inhibition of murine prostatic carcinoma growth by immunization with or administration of viable human umbilical vein endothelial cells and CRM197 BJMBR
Huiyong,Zhang; Yong,Lu; Didier,Mekoo; Yu,Zhang; Jing,Fang; Rongyue,Cao; Jingjing,Liu.
Vaccination with xenogeneic and syngeneic endothelial cells is effective for inhibiting tumor growth. Nontoxic diphtheria toxin (CRM197), as an immunogen or as a specific inhibitor of heparin-binding EGF-like growth factor, has shown promising antitumor activity. Therefore, immunization with or administration of viable human umbilical vein endothelial cells (HUVECs) combined with CRM197 could have an enhanced antitumor effect. Six-week-old C57BL/6J male mice were vaccinated with viable HUVECs, 1 x 10(6) viable HUVECs combined with 100 μg CRM197, or 100 μg CRM197 alone by ip injections once a week for 4 consecutive weeks. RM-1 cells (5 x 10(5)) were inoculated by sc injection as a preventive procedure. During the therapeutic procedure, 6-week-old male...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RM-1 prostatic carcinoma; CRM197; Immunization; Human umbilical vein endothelial cells.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200008
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Immunization by subcutaneous implants of polyester-polyurethane sponges coupled with antigen BJMBR
Lima,S.L.; Machado,C.B.; Pereira,M.A.C.; Cara,D.C.; Velarde,D.T.; Andrade,S.P.; Gontijo,C.M..
A new protocol is described for immunization of outbred Swiss mice. The procedure is based on subcutaneous implantation of antigen-coupled polyester-polyurethane sponges cut into disks of 10 mm in diameter vs 2 mm in thickness. Antigen coupling was performed by overnight incubation of the sponge with a solution of ovalbumin (Ova) (2 mg/ml) diluted in sodium carbonate buffer, pH 9.6. The amount of ovalbumin that was taken up by the sponge was between 71.4 to 82.5 µg. This was estimated by comparing the Ova absorbance at 280 nm in coating buffer solutions before and after incubation. To compare the efficiency of the proposed method, experimental groups immunized with the antigen in the presence of adjuvants (10 µg in Al(OH)3 or 100 µg in complete Freund's...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Sponge; Implant; Immunization; Vaccine.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400011
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Individual variation is the key to the development of a vaccine against Staphylococcus aureus: a comparative study between mice lineages BJMBR
dos Santos,D.P.; Muniz,I.P.R.; Queiroz,A.F.; Pereira,I.S.; Souza,M.P.A.; Lima,L.J.; Sousa,L.R.O.; Ribeiro,I.S.; Galantini,M.P.L.; Marques,L.M.; Figueiredo,T.B.; da Silva,R.A.A..
Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages. Immunized mice demonstrated that the intradermal immunization with S. aureus promoted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunization; Staphylococcus aureus; Mice; Air pouch; Antibodies.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500616
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Induction of neutralizing antibodies in mice immunized with scorpion toxins detoxified by liposomal entrapment BJMBR
The possibility of producing neutralizing antibodies against the lethal effects of scorpion toxins was evaluated in the mouse model by immunization with an immunogen devoid of toxicity. A toxic fraction (5 mg) from the venom of the scorpion Tityus serrulatus was entrapped in sphingomyelin-cholesterol liposomes. The liposomes were treated for 1 h at 37oC with a 1% (w/w) trypsin solution in 0.2 M sodium carbonate buffer, pH 8.3. This treatment led to a strong reduction in venom toxicity. Immunization was performed as follows: mice were injected sc with 20 µg of the liposome-entrapped toxic fraction on days 1 and 21 and a final injection (20 µg) was administered ip on day 36. After injection of the immunogen, all mice developed an IgG response which was shown...
Palavras-chave: Liposomes; Neutralizing antibodies; Tityus serrulatus; Immunization; Toxins.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700010
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Low cost production and purification of polyclonal antibodies to staphylococcal enterotoxin A Rev. Microbiol.
Oliveira,Tereza Cristina R.M. de; Hirooka,Elisa Yoko.
An immunization scheme for production of antiserum to staphylococcal enterotoxin A (SEA) is proposed. The reference method of Robbins and Bergdoll was modified to reduce the number of doses and the amount of toxin used per animal. The best immunization scheme used injections in days 0, 8, 24, 59, 62 and 67. The amount of toxin at each injection was 5, 6, 20, 50, 100 and 200<FONT FACE="Symbol">m</font>g, respectively. The total amount of toxin was 381<FONT FACE="Symbol">m</font>g, which corresponded to a reduction of 107<FONT FACE="Symbol">m</font>g in the amount of toxin for each animal when compared to the reference method. The average antiserum titer using the Optimum Sensitivity Plate - OSP was 1:60 and using ELISA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Enterotoxins; Detection; Immunization.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200006
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Migration of Schistosoma mansoni sambon (Trematoda, Schistosomatidae) from skin to lungs in immunized NZ rabbits (Lagomorpha, Leporidae) by autoradiographic analysis Zoologia
Pinto,R. Magalhães; Noronha,Dely; Almeida,Marília S. S; Katz,Naftale; Tendler,Miriam.
Skin to lung migration of Schistosoma mansoni Sambon, 1907 - (75 Se) - selenomethionine-labeled cercariae was tracked by tissue autoradiography on days 1, 4, 6, 8 after challenge, in rabbits immunized with a S. mansoni derived saline extract. Either in vaccinated animals or in those of the control unprimed group, the peak of skin schistosomula occurs 24hs after infection. Comparison between peaks of lungs migrating larvae showed that, in control animals, the increase of worm burden in this site, is detected on the 6th day post-infection, differing from immunized rabbits, in which this peak occurs on day 4, when skin and lungs counts are still equivalent, decreasing gradually, showing a different pattern of the S. mansoni migration and suggesting that main...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Schistosoma mansoni; Migration; Autoradiography; Immunization; Rabbits.
Ano: 1994 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751994000400006
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Occurrence of Haemophilus influenzae strains in three Brazilian states since the introduction of a conjugate Haemophilus influenzae type b vaccine BJMBR
de Almeida,A.E.C.C.; de Filippis,I.; Abreu,A.O. de; Ferreira,D.G.; Gemal,A.L.; Marzochi,K.B.F..
Few vaccines in history have induced such a dramatic decline in incidence over such a short period of time as the Haemophilus influenzae type b (Hib) conjugate. This vaccine was introduced in 1988 in the United States, but only in 1999 was Hib immunization introduced by the Brazilian Ministry of Health as part of the routine infant National Immunization Program. The authors analyzed 229 H. influenzae (Hi) isolates from Public Health Laboratories in three Brazilian states: Pernambuco (Northeast, N = 54), Santa Catarina (South, N = 19), and Rio de Janeiro (Southeast, N = 156). The isolates were collected from Brazilian children 0-10 years of age with meningitis and other infections from 1990 to 2003 and were part of the research collection of the National...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Haemophilus influenzae; Haemophilus influenzae non-b; Hib conjugate vaccine; Immunization; Hib meningitis.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500016
Registros recuperados: 27
Primeira ... 12 ... Última
 

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