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| Giffard, I; Charmantier, G; Grousset, E; Aujoulat, F; Castille, R. |
| The ontogeny of the digestive tract (DT) and of Na+/K+-ATPase localization was investigated during the early postembryonic development (from yolk sac larva to juvenile) of the euryhaline teleost Dicentrarchus labrax reared at two salinities: seawater and diluted seawater. Histology, electron microscopy and immunocytochemistry were used to determine the presence and differentiation of ion transporting cells. At hatching, the DT is an undifferentiated straight tube over the yolk sac. At the mouth opening (day 5), it comprises six segments: buccopharynx, esophagus, stomach, anterior intestine, posterior intestine and rectum, well differentiated at the juvenile stage (day 72). The enterocytes displayed ultrastructural features similar to those of... |
| Tipo: Text |
Palavras-chave: Osmoregulation; Na+/K+ ATPase; Immunolocalization; Fish larva; Enteric ionocyte. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-4653.pdf |
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| PAULA, C. M. P. de; LIMA, D. C.; SOUZA SOBRINHO, F. de; TECHIO, V. H.. |
| Abstract : This study identified and compared the frequency of homology and recombination hotspots in sexual diploid and tetraploid species, apomictic tetraploids and hybrids of Brachiaria, by immunolocalization of the radiation sensitive 51 (RAD51). Roots were fixed in 4% paraformaldehyde. We applied primary antibody (Rat anti-Arabidopsis thaliana RAD51) and detected with a secondary antibody (Goat anti-rat IgG FITC). The analysis of this protein showed diffuse signals at leptotene. In zygotene, signals of RAD51 were identified in unpaired chromosomes or partially paired and adjacent signals of RAD51 in paired chromosomes, confirming its close involvement with the search for chromosomal homology. During pachytene, RAD51 signals were located between... |
| Tipo: Separatas |
Palavras-chave: Crossover; Homology; Immunolocalization; Recombination hotspots.; Meiosis.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035705 |
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| Sellami, Imene; Charmantier, Guy; Naceur, Hachem B.; Kacem, Adnane; Lorin-nebel, Catherine. |
| Artemia salina is an extremophile species that tolerates a wide range of salinity, especially hypertonic media considered lethal for the majority of other aquatic species. In this study, A. salina cysts were hatched in the laboratory and nauplii were acclimated at three different salinities (60, 139 and 212 ppt). Once in the adult phase, their hemolymph osmolality was measured. The animals were strong hypo-osmoregulators in the entire range of tested salinities, with up to 10 fold lower hemolymph osmolalities than their surrounding environment. Immunostaining of Na+/K+-ATPase was done on sections and on whole body mounts of adults in order to localize the ionocytes in different organs. An intense Na+/K+-ATPase immunostaining throughout the cells was... |
| Tipo: Text |
Palavras-chave: Artemia salina; Hypo-osmoregulation; Na+/K+ -ATPase; Immunolocalization; Metepipodites; Maxillary glands. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00602/71426/69911.pdf |
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| BAHIA, D.; MORTARA, R. A.; KUSEL, J. R.; ANDRADE, L. F.; LUDOLF, F.; KUSER, P. R.; AVELAR, L.; TROLET, J.; DISSOUS, C.; PIERCE, R. J.; OLIVEIRA, G.. |
| Parasites. Identification of SmFes orthologues in other schistosoma species. Antibodies. Western blots. Immunofluorescence studies. Comparative protein structure modeling. SmFes homologues in other schistosome species. SmFes protein is differentially expressed during the parasite life cycle. Localization of SmFes in cercariae, schistosomula and miracidia. The role of the miracidial terebratorium. Modeling of the SmFes protein. |
| Tipo: Artigo de periódico |
Palavras-chave: Proteína tirosina quinases; Immunolocalization; Schistosoma Mansoni; Host-parasite relationships; Protein-tyrosine kinases. |
| Ano: 2007 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/4846 |
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| ALBUQUERQUE, E. V. S.; BEZERRA, C. A.; ROMERO, J. V.; VALENCIA, J. W. A.; VALENCIA-JIMÉNEZ, A.; PIMENTA, L. M.; BARBOSA, A. E. A. D.; SILVA, M. C. M.; MENEGUIM, A. M.; SÁ, M. E. L.; ENGLER, G.; ALMEIDA-ENGLER, J. de; FERNANDEZ, D.; GROSSI-DE-SA, M. F.. |
| Tipo: Separatas |
Palavras-chave: Fruit-specific expression; Immunolocalization; Coffea Arábica; Hypothenemus Hampei; Genetically modified plants. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032285 |
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| Brito,C.F.A.; Oliveira,G.C.; Oliveira,S.C.; Street,M.; Riengrojpitak,S.; Wilson,R.A.; Simpson,A.J.G.; Correa-Oliveira,R.. |
| Sm14 is a 14-kDa vaccine candidate antigen from Schistosoma mansoni that seems to be involved in cytoplasmic trafficking of fatty acids. Although schistosomes have a high requirement for lipids, they are not able to synthesize fatty acids and sterols de novo. Thus, they must acquire host lipids. In order to determine whether Sm14 is present in different stages of the life cycle of the parasite, we performed RT-PCR. Sm14 mRNA was identified in all stages of the life cycle studied, mainly schistosomulum, adult worm and egg. Additionally, we used a rabbit anti-Sm14 polyclonal antibody in an indirect immunofluorescence assay to localize Sm14 in adult worm sections. The basal lamella of the tegument and the gut epithelium were strongly labeled. These tissues... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Sm14; Fatty acid-binding protein; FABP; Immunolocalization; RT-PCR. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300014 |
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