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Registros recuperados: 7
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Digestive tract ontogeny of Dicentrarchus labrax: Implication in osmoregulation ArchiMer
Giffard, I; Charmantier, G; Grousset, E; Aujoulat, F; Castille, R.
The ontogeny of the digestive tract (DT) and of Na+/K+-ATPase localization was investigated during the early postembryonic development (from yolk sac larva to juvenile) of the euryhaline teleost Dicentrarchus labrax reared at two salinities: seawater and diluted seawater. Histology, electron microscopy and immunocytochemistry were used to determine the presence and differentiation of ion transporting cells. At hatching, the DT is an undifferentiated straight tube over the yolk sac. At the mouth opening (day 5), it comprises six segments: buccopharynx, esophagus, stomach, anterior intestine, posterior intestine and rectum, well differentiated at the juvenile stage (day 72). The enterocytes displayed ultrastructural features similar to those of...
Tipo: Text Palavras-chave: Osmoregulation; Na+/K+ ATPase; Immunolocalization; Fish larva; Enteric ionocyte.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-4653.pdf
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Frequency of the RAD51 (radiation sensitive 51) recombinase in species and hybrids of Brachiaria and relationship with genetic variability. Repositório Alice
PAULA, C. M. P. de; LIMA, D. C.; SOUZA SOBRINHO, F. de; TECHIO, V. H..
Abstract : This study identified and compared the frequency of homology and recombination hotspots in sexual diploid and tetraploid species, apomictic tetraploids and hybrids of Brachiaria, by immunolocalization of the radiation sensitive 51 (RAD51). Roots were fixed in 4% paraformaldehyde. We applied primary antibody (Rat anti-Arabidopsis thaliana RAD51) and detected with a secondary antibody (Goat anti-rat IgG FITC). The analysis of this protein showed diffuse signals at leptotene. In zygotene, signals of RAD51 were identified in unpaired chromosomes or partially paired and adjacent signals of RAD51 in paired chromosomes, confirming its close involvement with the search for chromosomal homology. During pachytene, RAD51 signals were located between...
Tipo: Separatas Palavras-chave: Crossover; Homology; Immunolocalization; Recombination hotspots.; Meiosis..
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035705
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Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia) ArchiMer
Sellami, Imene; Charmantier, Guy; Naceur, Hachem B.; Kacem, Adnane; Lorin-nebel, Catherine.
Artemia salina is an extremophile species that tolerates a wide range of salinity, especially hypertonic media considered lethal for the majority of other aquatic species. In this study, A. salina cysts were hatched in the laboratory and nauplii were acclimated at three different salinities (60, 139 and 212 ppt). Once in the adult phase, their hemolymph osmolality was measured. The animals were strong hypo-osmoregulators in the entire range of tested salinities, with up to 10 fold lower hemolymph osmolalities than their surrounding environment. Immunostaining of Na+/K+-ATPase was done on sections and on whole body mounts of adults in order to localize the ionocytes in different organs. An intense Na+/K+-ATPase immunostaining throughout the cells was...
Tipo: Text Palavras-chave: Artemia salina; Hypo-osmoregulation; Na+/K+ -ATPase; Immunolocalization; Metepipodites; Maxillary glands.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00602/71426/69911.pdf
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Schistosoma mansoni: expression of Fes-like tyrosine kinase SmFes in the tegument and terebratorium suggests its involvement in host penetration. Repositório Alice
BAHIA, D.; MORTARA, R. A.; KUSEL, J. R.; ANDRADE, L. F.; LUDOLF, F.; KUSER, P. R.; AVELAR, L.; TROLET, J.; DISSOUS, C.; PIERCE, R. J.; OLIVEIRA, G..
Parasites. Identification of SmFes orthologues in other schistosoma species. Antibodies. Western blots. Immunofluorescence studies. Comparative protein structure modeling. SmFes homologues in other schistosome species. SmFes protein is differentially expressed during the parasite life cycle. Localization of SmFes in cercariae, schistosomula and miracidia. The role of the miracidial terebratorium. Modeling of the SmFes protein.
Tipo: Artigo de periódico Palavras-chave: Proteína tirosina quinases; Immunolocalization; Schistosoma Mansoni; Host-parasite relationships; Protein-tyrosine kinases.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/4846
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Seed-specific stable expression of the alpha-AI1 inhibitor in coffee grains and the in vivo implications for the development of the coffee berry borer. Repositório Alice
ALBUQUERQUE, E. V. S.; BEZERRA, C. A.; ROMERO, J. V.; VALENCIA, J. W. A.; VALENCIA-JIMÉNEZ, A.; PIMENTA, L. M.; BARBOSA, A. E. A. D.; SILVA, M. C. M.; MENEGUIM, A. M.; SÁ, M. E. L.; ENGLER, G.; ALMEIDA-ENGLER, J. de; FERNANDEZ, D.; GROSSI-DE-SA, M. F..
Tipo: Separatas Palavras-chave: Fruit-specific expression; Immunolocalization; Coffea Arábica; Hypothenemus Hampei; Genetically modified plants.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032285
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Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm BJMBR
Brito,C.F.A.; Oliveira,G.C.; Oliveira,S.C.; Street,M.; Riengrojpitak,S.; Wilson,R.A.; Simpson,A.J.G.; Correa-Oliveira,R..
Sm14 is a 14-kDa vaccine candidate antigen from Schistosoma mansoni that seems to be involved in cytoplasmic trafficking of fatty acids. Although schistosomes have a high requirement for lipids, they are not able to synthesize fatty acids and sterols de novo. Thus, they must acquire host lipids. In order to determine whether Sm14 is present in different stages of the life cycle of the parasite, we performed RT-PCR. Sm14 mRNA was identified in all stages of the life cycle studied, mainly schistosomulum, adult worm and egg. Additionally, we used a rabbit anti-Sm14 polyclonal antibody in an indirect immunofluorescence assay to localize Sm14 in adult worm sections. The basal lamella of the tegument and the gut epithelium were strongly labeled. These tissues...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Sm14; Fatty acid-binding protein; FABP; Immunolocalization; RT-PCR.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300014
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Vicilins (7S storage globulins) of cowpea (Vigna unguiculata) seeds bind to chitinous structures of the midgut of Callosobruchus maculatus (Coleoptera: Bruchidae) larvae BJMBR
Sales,M.P.; Pimenta,P.P.; Paes,N.S.; Grossi-de-Sá,M.F.; Xavier-Filho,J..
The presence of chitin in midgut structures of Callosobruchus maculatus larvae was shown by chemical and immunocytochemical methods. Detection by Western blotting of cowpea (Vigna unguiculata) seed vicilins (7S storage proteins) bound to these structures suggested that C. maculatus-susceptible vicilins presented less staining when compared to C. maculatus-resistant vicilins. Storage proteins present in the microvilli in the larval midgut of the bruchid were recognized by immunolabeling of vicilins in the appropriate sections with immunogold conjugates. These labeling sites coincided with the sites labeled by an anti-chitin antibody. These results, taken together with those previously published showing that the lower rates of hydrolysis of variant vicilins...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bruchid beetles; Callosobruchus maculatus; Cowpea seeds; Vigna unguiculata; Vicilins (7S storage proteins); Immunolocalization; Midgut.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100003
Registros recuperados: 7
Primeira ... 1 ... Última
 

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