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Community-acquired pneumonia in the childhood: analysis of the diagnostic methods 55
Requejo,Henry I. Z..
Immunological assays such as CIE, LA, and Dot-ELISA were compared in order to diagnose community-acquired pneumonia. Serum, pleural fluid and urine samples were comparatively employed for bacterial antigen detection. Dot-ELISA proved to be an original and practical alternative procedure for detecting bacterial polysaccharide antigens from pleural fluid and/or concentrated urine samples, providing a rapid diagnosis for pediatric patients with community-acquired pneumonia.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Community-acquired pneumonia; Streptococcus pneumoniae; Haemophilus influenzae; Bacterial pneumonia; Immunological methods.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200016
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Methodology for fast evaluation of Bacillus thuringiensis crystal protein content 58
Alves,Lúcia M. Carareto; Lemos,Manoel V. F..
The development of the production and use of Bacillus thuringiensis in Brazil at a commercial scale faces certain difficulties, among them the establishment of efficient methodologies for the quantitation of toxic products to be commercialized. Presently, the amount of toxin is given in percentage by analyzing the samples total protein content. Such methodology however, does not measure the actual amount of active protein present in the product, since most strains express different endotoxin genes and might even produce b-toxin. Since the various types of toxins exhibit different antigenic characteristics, this work has as objective the utilization of fast immunological techniques to quantify the level of crystal protein. Crystal protein produced by a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis; Crystal protein; Immunological methods.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300012
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