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Registros recuperados: 27
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A putative RA-like region in the brain of the scale-backed antbird, Willisornis poecilinotus (Furnariides, Suboscines, Passeriformes, Thamnophilidae) Genet. Mol. Biol.
Lima,Jamily L.R. de; Soares,Fabricio A.; Remedios,Ana C.S.; Thom,Gregory; Wirthlin,Morgan; Aleixo,Alexandre; Schneider,Maria Paula C.; Mello,Claudio V.; Schneider,Patricia N..
The memorization and production of song in songbirds share important parallels with the process of speech acquisition in humans. In songbirds, these processes are dependent on a group of specialized telencephalic nuclei known as the song system: HVC (used as a proper name), RA (robust nucleus of arcopallium), LMAN (lateral magnocellular nucleus of the nidopallium) and striatal Area X. A recent study suggested that the arcopallium of the Sayornis phoebe, a non vocal learner suboscine species, contains a nucleus with some properties similar to those of songbird RA, suggesting that the song system may have been present in the last common ancestor of these groups. Here we report morphological and gene expression evidence that a region with some properties...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vocal learning; RGS4; Song nuclei; In situ hybridization.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300249
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Analysis of trophic responses in lesioned brain: focus on basic fibroblast growth factor mechanisms BJMBR
Chadi,G.; Fuxe,K..
The actions of fibroblast growth factors (FGFs), particularly the basic form (bFGF), have been described in a large number of cells and include mitogenicity, angiogenicity and wound repair. The present review discusses the presence of the bFGF protein and messenger RNA as well as the presence of the FGF receptor messenger RNA in the rodent brain by means of semiquantitative radioactive in situ hybridization in combination with immunohistochemistry. Chemical and mechanical injuries to the brain trigger a reduction in neurotransmitter synthesis and neuronal death which are accompanied by astroglial reaction. The altered synthesis of bFGF following brain lesions or stimulation was analyzed. Lesions of the central nervous system trigger bFGF gene expression by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Radioautography; In situ hybridization; Basic fibroblast growth factor; FGF receptor; Astrocyte; Image analysis; Central nervous system; Brain lesion.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200007
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Biogeochemistry and community composition of iron- and sulfur-precipitating microbial mats at the Chefren mud volcano (Nile Deep Sea fan, Eastern Mediterranean) ArchiMer
Omoregie, E; Mastalerz, V; De Lange, G; Straub, K; Kappler, A; Roy, H; Stadnitskaia, A; Foucher, Jean-paul; Boetius, A.
In this study we determined the composition and biogeochemistry of novel, brightly colored, white and orange microbial mats at the surface of a brine seep at the outer rim of the Chefren mud volcano. These mats were interspersed with one another, but their underlying sediment biogeochemistries differed considerably. Microscopy revealed that the white mats were granules composed of elemental S filaments, similar to those produced by the sulfide-oxidizing epsilonproteobacterium "Candidatus Arcobacter sulfidicus." Fluorescence in situ hybridization indicated that microorganisms targeted by a "Ca. Arcobacter sulfidicus"-specific oligonucleotide probe constituted up to 24% of the total the cells within these mats. Several 16S rRNA gene sequences from organisms...
Tipo: Text Palavras-chave: Gulf of mexico; Sulfate reducing bacteria; Egyptian continental margin; Northeast pacific ocean; Ribosomal rna sequences; In situ hybridization; Anaerobic oxidation; Marine sediments; Gas hydrate; Oxiding bacteria.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6201.pdf
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Bonamia ostreae, parasite de l'huître plate, Ostrea edulis: sa position taxonomique parmi les parasites du groupe "microcell", analyses des interactions hôte/parasite chez plusieurs populations d'huîtres plates ArchiMer
Besnard-cochennec, Nathalie.
Bonamia ostreae is the causative agent of hemocyte disease. It has been detected first during mortalities occured in Franc in 1979. Bonamiosis spread rapidly among oysters farming in France and Europ. Another species, Bonamia sp. has been detected in New Zealand and Australia. These paasites were both included in the "microcell" group. This group contains 2 others parasites, Mikrocytos mackini affecting Crassostrea gigas in British Columbia (Canada) and M. roughleyi affecing Saccostrea glomerata in Australia. Structural and molecular caracterisation of all these parasites were realised. Results show tha B. ostreae, B. sp., B. exitiosus. Molecular tools are available for genus detection, Bonamia spp. and Mikrocytos and species identification (B. ostreae, B....
Tipo: Text Palavras-chave: Bonamia ostreae; Ostrea edulis; Taxonomie; Diagnotic; PCR; In situ hybridation; Hémocytes; Lectines; Phagocytose; Résistance; Bonamia ostreae; Ostrea edulis; Taxonomy; Diagnotic; PCR; In situ hybridization; Hemocytes; Lectins; Phagocytosis; Resistance.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00211/32223/30671.pdf
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C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae) Genet. Mol. Biol.
Maffei,Eliane Mariza Dortas; Pompolo,Silvia das Graças; Petitpierre,Eduard.
The aim of this study was to describe mitotic and meiotic chromosomes of Cycloneda sanguinea using C-banding, fluorescent in situ hybridization (FISH) rDNA probes, and sequential FISH/Ag-NOR staining. The chromosome number was 2n = 18 + XX for females and 2n = 18 + Xy for males. The X chromosome was metacentric and the Y chromosome was very small. During meiosis, the karyotypic meioformula was n = 9 + Xy p, and sex chromosomes configured a parachute at metaphase I. At the beginning of pachytene, bivalents were still individualized, and sex chromosomes were associated end-to-end through the heteropycnotic region of the X chromosome. Later in pachytene, further condensation led to the formation of a pseudo-ring by the sex bivalent. All chromosomes showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In situ hybridization; RDNA sequences; Sequential FISH/Ag-NOR staining; C-banding; Cycloneda sanguinea.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200011
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Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain Genet. Mol. Biol.
Gong,Jie; Xie,Yinjie; Yu,Kun; Yang,Ya'nan; Huang,Huiyang; Ye,Haihui.
Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY gene contains seven exons, six introns, a TATA box and several transcription factor binding sites that were found in 5'-promoter region which is 1221 bp in length-time quantitative PCR analysis indicated that the expression level of SpCHY mRNA in hepatopancreas was significantly higher than that in other tissues. Immunocytochemistry and in situ hybridization...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chymotrypsin; Scylla paramamosain; Immune response; Immunocytochemistry; In situ hybridization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300010
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Chromosomal distribution and evolution of abundant retrotransposons in plants: gypsy elements in diploid and polyploid Brachiaria forage grasses. Repositório Alice
SANTOS, F. C.; VALLE, C. B. do; CHIARI, L..
Tipo: Artigo de periódico Palavras-chave: Genome organization; Transposable elements; Repetitive DNA.; Metaviridae; Centromeres; Fish; Genetics; Genomics; Grasses; Chromosomes; In situ hybridization; Retrotransposons; Transposons..
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1040145
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Comparison of ethylene carbonate and formamide as components of the hybridization mixture in FISH Scientia Agricola
Kalinka,Anna; Myśliwy,Monika; Achrem,Magdalena.
ABSTRACT The protocols for in situ hybridization (ISH) techniques can vary considerably; however, they usually include denaturation and hybridization steps. Denaturing compounds are used to reduce denaturation and hybridization temperature, which keeps the proper morphology of the preparation. Formamide is the most commonly used reagent in in situ hybridization to lower the melting temperature. The substitution of toxic formamide for a non-toxic ethylene carbonate at 20 % and 50 % concentration in the hybridization mixture helped obtain a high quality in situ hybridization result with two sequences characteristic for rye, JNK, and Bilby. The results after hybridization, with a duration of 90 min and 16 h, were identical when formamide or ethylene carbonate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Secale; In situ hybridization; Repeated sequences; Non-toxic.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000401101
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Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat ArchiMer
Segarra, Amelie; Baillon, Laury; Faury, Nicole; Tourbiez, Delphine; Renault, Tristan.
High mortality rates are reported in spat and larvae of Pacific oyster Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in France. Although the viral infection has been experimentally reproduced in oyster larvae and spat, little knowledge is currently available concerning the viral entry and its distribution in organs and tissues. This study compares OsHV-1 DNA and RNA detection and localization in experimentally infected oysters using two virus doses: a low dose that did not induce any mortality and a high dose inducing high mortality. Real time PCR demonstrated significant differences in terms of viral DNA amounts between the two virus doses. RNA transcripts were detected in oysters receiving the highest dose of viral...
Tipo: Text Palavras-chave: Crassostrea gigas; In situ hybridization; Ostreid herpesvirus 1; Viral DNA; Viral RNA.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00300/41099/40272.pdf
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Differentiation of BP-type baculovirus strains using in situ hybridization ArchiMer
Durand, S; Lightner, Dv; Bonami, Jr.
BP-type viruses infect wild and farmed shrimp species in the Americas and can cause high mortality in larval stages. Histologically, BP disease is characterized by the presence of occlusion bodies (OBs) in the nucleus of hepatopancreatic epithelial cells. By in situ hybridization using different molecular probes, we tested shrimp infected with BP-type viruses collected from several geographical areas. In one shrimp, a case of double infection by 2 different BP-type viruses was noted. This has not been previously reported. The molecular probes recognized only the Pacific strain of BP. This specificity of the probe was confirmed by in situ hybridization tests with some infected shrimp collected from the Atlantic and Pacific coasts. The probes reacted only...
Tipo: Text Palavras-chave: Baculovirus; BP; In situ hybridization; Nonradioactive probes; Shrimp.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/00447/55821/62253.pdf
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Ecologie du copépode calanoïde Paracartia grani. Implication dans le cycle de vie du parasite Marteilia refringens dans la lagune de Thau ArchiMer
Boyer, Séverine.
In this study, the life cycle of Paracartia grani, a calalloid copepod belonging to the Acartiidae family was determined in Thau lagoon. Indeed, the copepod involvement in the life cycle of the parasite Marteilia refringens affecting the bivalve productions is suspected. Mesozooplanktonic community .was monitored twice a month. over two years at a fixed station in the lagoon. Sampling has identified P. grani as the acartiid dominant species in summer. From April to January, the copepod is found in the water column while from February to early April it remains in the sediment as diapausing eggs. The analysis of the population structure (size spectmm, contribution of developmental stages and sex ratio) has revealed that there are in between 6 and 9...
Tipo: Text Palavras-chave: Paracartia grani; Cycle de vie; Production d'oeufs; Oeufs de diapause; Mésozooplancton; Marteilia refringens; Mytilus galloprovincialisa; Ruditapes decussatus; PCR; Hybridation in situ; Lagune de Thau; Paracartia grani; Life cycle; Egg production; Diapause eggs; Mesozooplankton; Marteilia refringens; Mytilus galloprovincialis; Ruditapes decussatus; PCR; In situ hybridization; Thau lagoon.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00118/22931/21482.pdf
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Epstein-Barr virus infection and gastric carcinoma in São Paulo State, Brazil BJMBR
Lopes,L.F.; Bacchi,M.M.; Elgui-de-Oliveira,D.; Zanati,S.G.; Alvarenga,M.; Bacchi,C.E..
Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and most people have serological evidence of previous viral infection at adult age. EBV is associated with infectious mononucleosis and human cancers, including some lymphomas and gastric carcinomas. Although EBV was first reported in lymphoepithelioma-like gastric carcinoma, the virus was also found in conventional adenocarcinomas. In the present study, 53 gastric carcinomas diagnosed in São Paulo State, Brazil, were evaluated for EBV infection by non-isotopic in situ hybridization with a biotinylated probe (Biotin-AGACACCGTCCTCACCACCC GGGACTTGTA) directed to the viral transcript EBER-I, which is actively expressed in EBV latently infected cells. EBV infection was found in 6 of 53 (11.32%) gastric...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epstein-Barr virus; Gastric carcinoma; In situ hybridization; Brazil.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100016
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First detection of OsHV-1 in the cephalopod Octopus vulgaris. Is the octopus a dead-end for OsHV-1? ArchiMer
Prado-alvarez, Maria; García-fernández, Pablo; Faury, Nicole; Azevedo, Carlos; Morga, Benjamin; Gestal, Camino.
The ostreid herpes virus (OsHV-1), associated with massive mortalities in the bivalve Crassostrea gigas, was detected for the first time in the cephalopod Octopus vulgaris. Wild adult animals from a natural breeding area in Spain showed an overall prevalence of detection of 87.5% between 2010 and 2015 suggesting an environmental source of viral material uptake. Overall positive PCR detections were significantly higher in adult animals (p = 0.031) compared to newly hatched paralarvae (62%). Prevalence in embryos reached 65%. Sequencing of positive amplicons revealed a match with the variant OsHV-1 µVar showing the genomic features that distinguish this variant in the ORF4. Gill tissues from adult animals were also processed for in situ hybridization and...
Tipo: Text Palavras-chave: Octopus vulgaris; Crassostrea gigas; OsHV-1; Gene expression; In situ hybridization.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00680/79201/81700.pdf
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First report of a Mikrocytos-like parasite in European oysters Ostrea edulis from Canada after transport and quarantine in France ArchiMer
Gagne, N; Cochennec, Nathalie; Stephenson, M; Mcgladdery, S; Meyer, G; Bower, S.
As part of a disease resistance experiment, 112 apparently healthy European flat oysters Ostrea edulis L. were exported from Canada (Nova Scotia) into France to test their susceptibility to Bonamia ostreae infection. Twelve oysters died in transit and 17 others died within 2 wk of laboratory quarantine acclimation. All oysters were examined histologically, and the 17 that died during quarantine were assayed for microcells (Bonamia sp. and Mikrocytos mackini) using molecular techniques. A microcell parasite was detected in the connective tissue of 5 of the 112 oysters. Morphological appearance, tissue affinity and molecular characterization through PCR, in situ hybridization (ISH), fluorescence in situ hybridization (FISH) and sequencing revealed a protist...
Tipo: Text Palavras-chave: Histology; PCR; In situ hybridization; Ostrea edulis; Mikrocytos.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4475.pdf
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Homoeologous chromosome pairing and alien introgression analyses in wide-cross derivatives of Oryza through flourescence in situ hybridization International Rice Research Institute
Nguyen, Thi Ngoc Hue.
xiv, 157 l. : col. ill. Thesis (Ph.D.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Chromosome pairing; Introgression; In situ hybridization; Hybrids; Plant genetics; Plant breeding.
Ano: 2004 URL: http://hdl.handle.net/123456789/384
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Homoeologous relationships among genomes of cultivated and wild species of Oryza characterized through genomic in situ hybridization International Rice Research Institute
Dhillon, Bhupinder Pal Singh.
xiv, 99 l. : col. ill. Thesis (M.S.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Plant genetics; Genome; Varieties; Wild relatives; In situ hybridization; Hybrids.
Ano: 2001 URL: http://hdl.handle.net/123456789/485
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In situ hybridization detection of initial infective stages of Marteilia refringens (Paramyxea) in its host Mytilus galloprovincialis ArchiMer
Carrasco, N; Arzul, Isabelle; Berthe, Franck; Furones, M.
Tipo: Text Palavras-chave: Mussel; Marteilia refringens; In situ hybridization; Gills; Early stages; Connective tissue.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3941.pdf
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In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio) Biol. Res.
LÓPEZ,MAURICIO; FIGUEROA,JAIME; KAUSEL,GUDRUN; VERA,MARÍA INÉS; KRAUSKOPF,MANUEL.
We isolated and cloned a carp somatolactin SL DNA fragment, of which 78% of the nucleotides were identical to the corresponding salmon SL sequence. The results obtained upon Northern blot hybridization of carp pituitary RNA allowed the identification of two transcripts as described for other fish. When the content of SL transcripts in pituitary sections from summer- and winter- acclimatized carp was quantified by in situ hybridization assays, we found no significant differences between the two seasons. In salmonids, plasma SL reaches higher levels in summer than in winter in synchrony with the water temperature cycle; in the eurythermal carp, however, the complex adaptive responses imposed by seasonal environmental changes do not seem to include the...
Tipo: Journal article Palavras-chave: Acclimatization; In situ hybridization; Somatolactin expression; Pituitary; Carp; Fish.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100010
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Influence of the neural tube/notochord complex on MyoD expression and cellular proliferation in chicken embryos BJMBR
Alves,H.J.; Alvares,L.E.; Gabriel,J.E.; Coutinho,L.L..
Important advances have been made in understanding the genetic processes that control skeletal muscle formation. Studies conducted on quails detected a delay in the myogenic program of animals selected for high growth rates. These studies have led to the hypothesis that a delay in myogenesis would allow somitic cells to proliferate longer and consequently increase the number of embryonic myoblasts. To test this hypothesis, recently segmented somites and part of the unsegmented paraxial mesoderm were separated from the neural tube/notochord complex in HH12 chicken embryos. In situ hybridization and competitive RT-PCR revealed that MyoD transcripts, which are responsible for myoblast determination, were absent in somites separated from neural tube/notochord...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken development; MyoD; Myogenesis; In situ hybridization; BrdU; Cellular proliferation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200005
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Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids PAB
Santos,Fernanda Motta da Costa; Torres,Giovana Augusta; Techio,Vânia Helena; Pereira,Antônio Vander; Davide,Lisete Chamma.
Abstract: The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pennisetum glaucum; Pennisetum purpureum; In situ hybridization; Intergenomic pairing; Interspecific hybridization.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000900814
Registros recuperados: 27
Primeira ... 12 ... Última
 

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