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| Valente,Tiago Neves Pereira; Detmann,Edenio; Queiroz,Augusto César de; Valadares Filho,Sebastião de Campos; Gomes,Daiany Iris; Figueiras,Janderson Florêncio. |
| The objective of this study was to evaluate the in situ degradation profiles of dry matter (DM) and neutral detergent fiber (NDF) of different forages using nylon (50 µm), F57 (Ankom®) and non-woven textile (NWT - 100 g/m²) bags. Eight forage samples were used: sugarcane, corn silage, elephant grass cut at 50 and 250 days of regrowth, corn straw, signal grass hay, coast cross hay, and fresh alfalfa. Samples were incubated for 0, 3, 6, 12, 18, 24, 48, 72, 96, 120, 144, 168, 192, 216, 240, and 312 hours. Two bags of each textile were used at each incubation time, totaling 768 bags, using two crossbred Holstein × Zebu steers fitted with ruminal canullae. There was difference in the common rate of lag and degradation (λ) of DM for all forages, except for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: F57; In situ method; Non-woven textile; Nylon. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011001100039 |
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| Velásquez,Alejandro; Pichard,Gastón. |
| Proteolytic activity of enzymatic extracts generated from rumen microorganisms cultivated in vitro was evaluated. The incubation of rumen fluid used different substrates to generate a higher enzyme concentration and promote a broad spectrum of hydrolytic activity. The composition of the substrates used in the cultivation of the fluid was enriched in protein, starch or cell wall. Enzyme preparations were evaluated by incubating in 30 mL of buffer 50 mM Tris-HCl (pH 6.5) at 39 °C during 48 hours, 100 mg of crude protein from feeds soybean meal, canola meal, sunflower meal, gluten feed, dehydrated alfalfa meal, berseem clover, oat forage and perennial ryegrass. Enzyme extracts from cultivated rumen fluid showed an average protein breakdown of 75.5%, in eight... |
| Tipo: Journal article |
Palavras-chave: In vitro rumen proteolysis; Ruminal enzymes; Streptomyces griseus; In situ method. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000300005 |
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