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Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp. Ciencia e Investigación Agraria
Bastías,Adriana; Almada,Rubén; Rojas,Pamela; Donoso,José Manuel; Hinrichsen,Patricio; Sagredo,Boris.
A. Bastías, R. Almada, P. Rojas, J.M. Donoso, P. Hinrichsen, and B. Sagredo. 2016. Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp. Cien. Inv. Agr. 43(3):429-441. In vitro culture is a very popular technique to mass propagate valuable plant genotypes, including Prunus sp. cultivars. Plants that undergo tissue culture processes often change their morphology and behavior due to the "rejuvenation" caused by the plant growth regulators included in the medium. To evaluate the effects of rejuvenation by tissue culture in Prunus sp., the expression patterns of the aging gene pathway described in plant models, which include the highly conserved microRNA (miRNA or miR) 156/157 and 172...
Tipo: Journal article Palavras-chave: Development; In vitro culture; Hase change; Rejuvenation.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300009
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Anatomical and histochemical aspects of zigotic embryo and leaves in 'Coqueiro Anão' Ciência Rural
Santana,Marlucia Cruz de; Souza,Margarete Magalhães; Pereira,Telma Nair Santana; Teixeira,Sílvio Lopes.
The paper provides information about histochemical staining reactions in leaves and embryos of 'Coqueiro Anão' (Cocos nucifera). It was compared in vitro coconut and autotrophic palm leaves. Reactions for insoluble polysaccharides and acidic compounds, protein, extractable lipids, lignin and other classes of compounds were tested using histochemical tests. None sample gave positive reaction for lignin and phenolic compounds. All the samples gave positive reaction for protein, starch and insoluble polysaccharides while acidic compounds were positive only in in vitro leaves. Both in vitro and autotrophic leaves gave positive reaction for lipids showing presence of cuticle even in in vitro leaves. Only autotrophic palm leaves showed idioblasts containing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocos nucifera; In vitro culture; Polysaccharides; Protein; Lipids.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400019
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Asymbiotic culture of Cattleya intermedia Graham (Orchidaceae): the influence of macronutrient salts and sucrose concentrations on survival and development of plantlets Acta Botanica
Sasamori,Márcio Hisayuki; Endres Júnior,Delio; Droste,Annette.
ABSTRACTCattleya intermediais an Atlantic Forest species endemic to Brazil that is classed as vulnerable on the list of threatened species. In this study, C. intermedia plantlets were micropropagated in an asymbiotic culture and the influence of different concentrations of sucrose (15, 30, 45 and 60 g L-1, plus a zero sucrose medium) and macronutrient salts (complete Murashige and Skoog (MS) medium and half MS medium (with half-strength macronutrients)) on survival and development of the plantlets was evaluated. In all treatments 100% plantlet survival was achieved. The integrated analysis of height of aerial part, number of leaves per plantlet, fresh mass, number of roots per plantlet and length of the longest root showed that the plantlets exhibited...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atlantic Forest; Carbohydrate; Conservation; In vitro culture; Orchids; Propagation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300292
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Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae) Rev. Ciênc. Agron.
Castro,Ana Hortência Fonsêca; Braga,Karina de Queiroz; Sousa,Fernanda Marinez de; Coimbra,Mairon César; Chagas,Rafael César Russo.
ABSTRACT This study developed a methodology for callus induction in leaf segments of B. verbascifolia and evaluated the bioactive phenolic compounds production. Leaf explants were cultured in MS medium with 30 g L-1 sucrose, solidified with 7 g L- 1 agar supplemented with 2,4-D (0; 4.52; 9.05; 18.10 µM) and BAP (0; 4.44; 8.88; 17.75 µM ) in the presence and absence of light. Forty-five days after inoculation we assessed the percentage of callus induction, color, consistency, fresh and dry matter, total phenols, flavonoids, tannins contents, and chromatographic profile by HPLC-DAD method. Callus induction occurred only in medium with growth regulators. Maximal induction (100%) was found in medium containing 2,4-D combined with BAP in the presence and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; Medicinal plant; In vitro culture; Secondary metabolites.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902016000100143
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Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae) Ciência Rural
Galdiano Júnior,Renato Fernandes; Mantovani,Cibele; Lemos,Eliana Gertrudes de Macedo.
ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro culture; Native orchid; Glucose; Sucrose; Acclimatization.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700203
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Cattleya walkeriana growth in different micropropagation systems Ciência Rural
Moreira,André Luís; Silva,Adriano Bortolotti da; Santos,Aline; Reis,Caroline Oliveira dos; Landgraf,Paulo Roberto Correa.
The aim of the present research was to verify the in vitro growth of orchids in different systems of micropropagation, being cultivated in a bioreactor, with natural ventilation and conventional systems. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. After 8 months, seedlings with 1 cm of length were placed in a culture vessel according to the treatments, which counted with two micropropagation systems (conventional and natural ventilation) in three media of culture (liquid, solid with 5 or 6g L-1 of agar). Two additional treatments in bioreactor of temporary and continuous immersion were performed. The design was entirely randomized (ERD), consisting of a 2x3 factorial with two additional treatments, totaling 8...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioreactor; Temporary immersion; Liquid medium; Natural ventilation; In vitro culture.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001000012
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Comparative Evaluation of Mehods for the Diagnosis of Bovine Tritrichomonas foetus Infection OAK
Felleisen, Richard S. J.; Schimid-Lambelet, Natacha; Walubengo, J..
Palavras-chave: Diagnosis; In vitro culture; Polymerase chain reaction (PCR).
Ano: 1997 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/266
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Comparative study of lipophilic and hydrophilic antioxidants from in vivo and in vitro grown Coriandrum sativum. IPB - Escola Superior Agrária
Dias, Maria Inês; Barros, Lillian; Sousa, Maria João; Ferreira, Isabel C.F.R..
Coriander is commonly used for medicinal purposes, food applications, cosmetics and perfumes. Herein, the production of antioxidants in vegetative parts (leaves and stems) of in vivo and in vitro grown samples was compared. In vitro samples were clone A- with notorious purple pigmentation in stems and leaves and clone B- green. Seeds were also studied as they are used to obtain in vivo and in vitro vegetative parts. Lipophilic (tocopherols, carotenoids and chlorophylls) and hydrophilic (sugars, ascorbic acid, phenolics, flavonols and anthocyanins) compounds were quantified. The antioxidant activity was evaluated by radical scavenging activity, reducing power and lipid peroxidation inhibition. The in vivo sample showed the highest antioxidant activity...
Tipo: Article Palavras-chave: Antioxidants; Coriandrum sativum; In vitro culture; Lipid peroxidation inhibition; Micropropagation; Radical scavenging activity.
Ano: 2011 URL: http://hdl.handle.net/10198/6242
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Continuous in vitro Cultivation of Babesia ovata OAK
Igarashi, Ikuo; Avarzed, Avgaandorjiin; Tanaka, Tetsuya; Inoue, Noboru; Ito, Masanari; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi.
Palavras-chave: Babesia ovata; In vitro culture; Low oxygen atmosphere; Cryopreservation.
Ano: 1994 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/212
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Cultura in vitro de embriões zigóticos de cupuaçuzeiro. Infoteca-e
LEDO, A. da S.; LAMEIRA, O. A.; MOURA, E.; MENEZES, I. C. de.
bitstream/item/42519/1/Boletim-Pesquisa-28.pdf
Tipo: Séries anteriores (INFOTECA-E) Palavras-chave: Cupuaçuzeiro; Belém; Pará; Brasil.; Cultura de Tecido; Cultura In Vitro; Micropropagação; Theobroma Grandiflorum.; Amazonia; In vitro culture; Micropropagation; Tissue culture..
Ano: 2000 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/382729
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Dry matter partitioning differences between shoots and roots in two contrasting genotypes of orchids and their relationship with endogenous levels of auxins, cytokinins and abscisic acid Rev. Bras. Fisiol. Veg.
PERES,LÁZARO E. P.; MAJEROWICZ,NIDIA; KERBAUY,GILBERTO B..
Epiphytic orchids have been considered an interesting model for plant development and may have unusual features related to the hormonal control of shoot and root growth. In the present experiment we have studied two genotypes of Catasetum fimbriatum Lindl. (Orchidaceae) with an inverse pattern of shoot and root dry matter accumulation. Whereas the CFC1 genotype directs 60% of dry matter towards the shoot system, this same amount is directed towards the root system in the CFC4 genotype. Differences in the endogenous content of cytokinins, IAA and ABA, as well as differences in IAA conjugation and oxidation in shoots and roots were observed in the two genotypes. These differences were correlated with their dry matter partitioning patterns. Thus, the genotype...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epiphytes; Development; In vitro culture; Orchids; Plant hormones.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000200007
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Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of Cattleya tigrina A. Richard Biological Sciences
Almeida, Vivian; Fraga, Hugo Pacheco de Freitas; Navarro, Bruno Bachiega; Guerra, Miguel Pedro; Pescador, Rosete.
Cattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with that, an efficient protocol for in vitro propagation of this species is proposed. The first evidences of PLBs induction in C. tigrina occurred at seven days in culture, starting from the basal portion of the leaf abaxial surface. A hypomethylation marked the beginning of cell differentiation, followed by an increased global DNA methylation at 35 days in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.03.00.00-0; 2.03.03.00-9; 2.03.06.00-8 orchids; Micropropagation; In vitro culture; Epigenetics; PLBs. Botânica.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36656
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Effect of somatotropin on survival and diameter of bovine preantral follicles Arq. Bras. Med. Vet. Zootec.
Silva,T.F.; Costa,S.L.; Costa,E.P.; Guimarães,J.D.; Queiroz-Castro,V.L.D..
ABSTRACT The aim of this study was to evaluate the effect of Recombinant bovine somatotropin (rbST) on survival and diameter of bovine preantral ovarian follicles (PAOF) cultured in vitro. Ovaries were collected from adult cows and fragments of ovarian cortex were immediately fixed (non-cultured control) or cultured in vitro in α-MEM+ alone or containing 10, 50, 100 or 1,000ng/mL rbST. The fragments were processed for Classical Histology and Transmission Electron Microscopy. After one and seven days of culture, the percentage of normal follicles in the non-cultured control was superior (P< 0.05) to the follicles cultured in α-MEM+ alone or with different rbST concentrations. The oocyte and follicular mean diameter did not increase during the culture for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; In vitro culture; Somatotropin; Preantral follicles.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501445
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Effect of supplementation of green tea polyphenols on the developmental competence of bovine oocytes in vitro BJMBR
Wang,Z.G.; Yu,S.D.; Xu,Z.R..
The objective of the present study was to examine the effect of green tea polyphenols (GTPs) supplementation during in vitro maturation, in vitro fertilization, and in vitro culture on the developmental competence of bovine oocytes. Cumulus-oocyte complexes aspirated from the ovaries were matured in vitro (38.5ºC for 24 h) and fertilized (38.5ºC for 15-18 h) and embryos were cultured (38.5ºC for 192 h) in a defined conditioned medium with or without GTPs supplementation. The GTPs used in the present study contained 99% catechin derivatives, with the major components being 50% (-)-epigallocatechin gallate, 22% (-)-epicatechin gallate, 18% (-)-epigallocatechin, and 10% (-)-epicatechin. Four replicate trials were done for each type of experiment. GTPs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine oocytes; In vitro culture; Green tea polyphenols; Embryo development.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800008
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Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae Ciencia e Investigación Agraria
Suárez-Sánchez,Juan; Alatorre-Rosas,Raquel; Poggi-Varaldo,Héctor M; Barrera-Cortés,Josefina.
The effect of inoculum size on the axenic in vitro production of Steinernema carpocapsae, using a transparent medium with hemoglobin and cholesterol, was studied. Axenic cultivation was performed with fertile eggs ofS. carpocapsae (hatching of eggs: 89 ± 4%), which were obtained by lyses of female nematodes and subsequent incubation. Two important aspects of this work include the use of a completely transparent medium enriched with hemoglobin and cholesterol and the high multiplication factor of the population (MFIP) of 87, which is considered high for in vitro axenic cultures of nematodes. In vivo methods have the advantage of high MFIP, but they also have the disadvantage of the high number and complexity of the operations involved for the purposes of...
Tipo: Journal article Palavras-chave: Axenic culture; Hemoglobin; In vitro culture; Steinernema carpocapsae; Transparent medium.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000100011
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Effects of green tea polyphenols, insulin-like growth factor I and glucose on developmental competence of bovine oocytes R. Bras. Zootec.
Wang,Zhengguang; Fu,Chunquan; Yu,Songdong.
The present study examined the effects of green tea polyphenols (GTP), insulin-like growth factor-I (IGF-I) and glucose on oocyte in vitro maturation, subsequent embryo development and blastocyst quality in bovine. Cumulus-oocyte complexes (COC) were aspirated from the ovaries and cultured in synthetic oviduct fluid supplemented with MEM amino acids (SOFaa) media supplemented with one of the following supplements: GTP (0, 10, 15 and 20 µM), IGF-I (0, 50, 100 and 150 ng/mL) or glucose (0, 1.5, 5.6 and 20 mM) for 24 h. The results showed that oocytes cultured in media supplemented with 15 µM GTP, 100 ng/mL IGF-I and 5.6 mM glucose, in separate experiments, have higher cleavage and blastocyst rates compared with oocytes cultured in media without or with other...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blastocyst quality; Embryo development; In vitro culture; In vitro maturation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200006
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Effects of wild plants essential oils on the growth of Phytophthra cinnamomi and Castanea sativa IPB - Escola Superior Agrária
Sousa, Maria João; Martins, Fátima; Belo, Hélio; Choupina, Altino; Martins, Anabela.
Wild plant essential oil effects on the growth control of Phytophthora cinnamomi and Castanea sativa M. João Sousa1, Fátima Martins1, Hélio Belo1, Altino Choupina1, 2 and Anabela Martins1 1Instituto Politécnico de Bragança, Escola Superior Agrária, Campus de Santa Apolónia, Apartado 1172, 5301-854 Bragança, Portugal 2CIMO- Centro de Investigação de Montanha, Campus de Santa Apolónia, Apartado 1172, 5301-854 Bragança, Portugal Corresponding author: joaos@ipb.pt In regions that have chestnut as the main economic resource such as in Bragança, a region in the North of Portugal, plant diseases caused by Phytophthora cinnamomi namely the chestnut ink disease are the reason for major economic losses. The prospect of an active natural product agent has a great...
Tipo: ConferenceObject Palavras-chave: Phytophthora cinnamomi; In vitro culture; Mentha; Essential oils.
Ano: 2010 URL: http://hdl.handle.net/10198/5049
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Efficient In Vitro Somatic Embryogenesis and Plant Regeneration from Mature and Immature Embryos of Wheat ( Triticum aestivum L.) BABT
Ahmadpour,Roghayeh; Zare,Nasser; Asghari-Zakarta,Rasool; Sheikhzadeh,Parisa.
ABSTRACT An efficient regeneration system is a pre-requisite for the application of genetic transformation and functional genomics study of important plants. In this study, the effect of different factors (plant growth regulators, casein hydrolysate, aspartic acid and ascorbic acid) on in vitro embryogenesis and regeneration of Arta, Bahar and Zagros cultivars from mature and immature explants were investigated. Immature and mature embryos were dissected from disinfected seeds 20-25 days after pollination and imbedded mature seeds, respectively, and cultured on MS (Murashige and Skoog) medium supplemented with different compounds. The results showed that immature embryos expose high capacity of embryogenesis and regeneration in comparison with mature...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro culture; Plant growth regulators; Somatic embryogenesis; Wheat.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100206
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Embriogênese somática e regeneração de plantas em açaizeiro. Infoteca-e
LEDO, A. da S.; LAMEIRA, O. A.; MENEZES, I. C. de.
O objetivo do presente trabalho foi estudar as diferentes respostas morfogenéticas de embriões zigóticos de açaizeiro (Euterpe oleracea Mart.) submetidos a várias condições de cultura in vitro. Os experimentos foram conduzidos em laboratório com material vegetal coletado de plantas de açaí na Embrapa Amazônia Oriental, Belém, PA, Brasil. Verificou-se a expressão de um modelo de embriogênese somática, direto, repetitivo a assincronizado em embriões zigóticos maduros cultivados em meio primário MS suplementado com 339.36 ? M de 2,4-diclorofenoxiacético (2,4-D) e transferidos para meio secundário MS na presença de 0,537 ? M de ácido 1-naftalenoacético (ANA) e 12,30 ? M de 2-isopenteniladenina (2iP). A conversão de embriões somáticos em plântulas foi alcançada...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Açaí de touceira; Embrapa Amazônia Oriental; Belém (PA); Pará; Eastern Amazon; Amazônia Oriental; Micropropagación.; Cultivo in vitro; Fitomejoramiento; Embriogénesis; Melhoramento genético vegetal; Açaí; Euterpe oleracea; Cultura in vitro; Embriogênese.; Micropropagação; Embryogenesis.; Micropropagation; Plant breeding; In vitro culture.
Ano: 2002 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/496030
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Embryogenic Callus induction on the scutellum and regeneration of plants as basis for genetic transformation of spring wheath (Triticum Aestivum L.) cultivars from Argentina JBAG
Souza Canada,E. D; Beck,E.
An effective tissue culture is an indispensable basis for the production of transgenic plants. In this study, we investigated the induction of embryogenic callus production from the scutellum of spring wheat and subsequent regeneration of plants. Twenty-two Argentine spring wheat varieties from three breeders were screened to select promising genotypes for the optimization of the procedure. Experimental variables were the developmental stage of the immature embryos and the concentration of the growth regulator 2,4-dichlorophenoxyacetic acid (2,4-D). Two developmental stages, termed H and W, respectively (according to He et al., 1986), were selected, and their performance in culture media containing high and low concentrations of 2,4-D was examined....
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro culture; Immature embryos; Embryogenic scutellar callus; Callus proliferation.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332013000300006
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