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Anti-nutritional factors and digestibility of protein in Cayocar brasiliense seeds Ciênc. Tecnol. Aliment.
SILVESTRINI,Virgínia Campos; GONÇALVES,Daniel Bonoto; GRANJEIRO,Paulo Afonso; SILVA,José Antonio da.
Abstract The Brazilian Cerrado presents a wide variety of natural products, including the Pequi (Caryocar brasiliense). The important factor that determines the dietary and nutritional ideality of protein is degestitibility. This work aims to evaluate the protein digestibility of Pequi seeds and the presence of anti-nutritional factors. The protein Pequi almonds were extracted, toasted and untoasted. Evaluation digestibility was structurally characterized by SDS-PAGE 15%, which can be used to analyze simulated gastric fluid (SFG) and digestion in intestinal fluid (SFI) in the presence of pepsin and trypsin/chymotrypsin. The extract from Pequi almonds showed inhibitory activity and was not detected hemagglutination. The intensity of the bands according to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caryocar brasiliense; Almonds; In vitro digestibility; 2S albumin; Peptidase inhibitor; Lectins.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000400632
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Avaliação do gênero Leucaena na região semi-árida de Pernambuco. Infoteca-e
SILVA, C. M. M. S..
Foram avaliadas no Banco Ativo de Germoplasma de Forrageiras do Centro de Pesquisa Agropecuaria do Tropico Semi-Arido- CPATSA da EMBRAPA, oito cultivares de leucena de diferentes procedencias. Foram avaliados com relacao a producao da materia seca, na fracao comestivel, digestibilidade in vitro da materia seca, para diferentes cultivares. A composicao mineral da fracao comestivel de leucena, para macro e micronutrientes. A leucena, na zona semi-arida de Pernambuco, pode proporcionar um suplemento forrageiro de alta qualidade.
Tipo: Folhetos Palavras-chave: Leucena; Composição mineral; Pernambuco; Mineral composition; Macro-nutrient; Micro-nutrient; Pasture; Pastagem; Digestibilidade In Vitro; Leucaena; Leucaena Leucocephala; Matéria Seca; Produtividade; In vitro digestibility; Pastures.
Ano: 1992 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/133239
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Bromatological evaluation of eleven corn cultivars harvested at two cutting heights R. Bras. Zootec.
Caetano,Hamilton; Oliveira,Mauro Dal Secco de; Freitas Júnior,José Esler de; Rêgo,Aníbal Coutinho do; Carvalho,Marina Vieira de; Rennó,Francisco Palma.
The objective of this study was to evaluate the chemical composition and dry matter in vitro digestibility of stem, leaf, straw, cob and kernel fractions of eleven corn (Zea mays) cultivars, harvested at two cutting heights. The experiment was designed as randomized blocks, with three replicates, in a 2 × 11 factorial arrangement (eleven cultivars and two cutting heights). The corn cultivars evaluated were D 766, D 657, D 1000, P 3021, P 3041, C 805, C 333, AG 5011, FOR 01, CO 9621 and BR 205, harvested at a low cutting height (5 cm above ground) and a high cutting height (5 cm below the first ear insertion). Cutting height influenced the dry matter content of the stem fraction, which was lower (23.95%) in plants harvested at the low, than in plants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Forage; In vitro digestibility; Ruminant; Zea mays.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000100002
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Chemical composition and fermentation characteristics of elephant grass silage with biodiesel industry co-products Ciência Rural
Cleef,Eric Haydt Castello Branco van; Silva Filho,José Cleto da; Neiva Júnior,Arnaldo Prata; Patiño Pardo,René Maurício; Rêgo,Aníbal Coutinho do; Gonçalves,Josemir de Souza.
The objective of this research was to evaluate the effect of three concentrations (3, 6, and 9%) of forage turnip (Raphanus sativus) and physic nut (Jatropha curcas) cakes on dry matter, crude protein, ether extract, neutral detergent fiber, acid detergent fiber, lignin, acid detergent insoluble nitrogen neutral detergent insoluble nitrogen contents, in vitro dry matter digestibility, pH values and concentrations of N-NH3 in elephant grass silages. It was used an entirely randomized design in factorial arrangement [(2×3)+1]. Experimental PVC silos were used and ensiled material was kept for 62 days. The addition of cakes increased the dry matter contents (P<0.05). The fibrous fractions were reduced (P<0.05) with the inclusion of cakes during the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ensilage; In vitro digestibility; Jatropha curcas; Pennisetum purpureum; Raphanus sativus.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000400024
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Coeficiente de digestibilidade in vitro e teores de proteina bruta, calcio e fosforo da grama-tio-pedro (Paspalum oteroi) no Pantanal Mato-Grossense. Infoteca-e
POTT, E. B..
A grama tio-pedro (Paspalum oteroi Swallen), submetida a intervalos entre cortes de 28, 56, 84 e 112 dias, apresentou, respectivamente, coeficientes de digestibilidade in vitro de 57,21%, 48,37%, 47,66% e 49,88%; teor de proteina bruta de 8,26%, 7,12%, 6,00% e 5,44%; teor de calcio de 0,50%, 0,56%, 0,58% e 0,35%; e teor de fosforo de 0,15%, 0,13%, 0,20% e 0,14%.
Tipo: Folhetos Palavras-chave: Forrageira nativa; Regiao tropical; Mato Grosso do Sul; Paspalum oteroi; Minerais; Plant; Native forage; Pasture; Analysis; Análise; Digestibilidade In Vitro; Gramínea; Pastagem; Planta; Pantanal; Grasses; In vitro digestibility; Minerals.
Ano: 1982 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/787710
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Concentración óptima y tóxica de cobre para el crecimiento de bacterias ruminales. Colegio de Postgraduados
Cervantes Gómez, Daniel.
El cobre (Cu) es un micromineral que cumple funciones esenciales en el organismo de los animales; del mismo modo es necesario para las bacterias ruminales, implicando que todos los nutrientes que requieren los microrganismos, dentro de estos el cobre, deben estar disponibles en el rumen en forma estable y continúa para asegurar su crecimiento. Por lo anterior, en el presente estudio se desarrollaron dos ensayos experimentales para determinar la concentración óptima y tóxica de cobre, con base al crecimiento y la actividad de las bacterias ruminales; en el primer experimento se utilizaron seis concentraciones de cobre (0, 10, 20, 30, 40 y 50 ppm), en un medio de cultivo que contenía heno de alfalfa. Los resultados de este ensayo mostraron que las...
Palavras-chave: Cobre; Concentración óptima y tóxica; Bacterias ruminales; Digestibilidad in vitro; Copper; Optimal and toxic concentration; Ruminal microbes; In vitro digestibility; Ganadería; Maestría.
Ano: 2012 URL: http://hdl.handle.net/10521/774
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Effect of chickpea (Cicer arietinum L.) germination on the major globulin content and in vitro digestibility Ciênc. Tecnol. Aliment.
Portari,Guilherme Vanucchi; Tavano,Olga Luisa; Silva,Maraiza A. da; Neves,Valdir Augusto.
Chickpea seed germination was carried out over a period of 6 days. Little variation in the nitrogen and total globulin content was observed. The major globulin (11 S type) showed higher variation after the 4th day of germination. The elution behaviour and distribution of the isolated major globulin fraction on Sepharose CL-6B chromatography showed little modification at the end of germination. On SDS-PAGE the peak eluted from Sepharose CL-6B showed changes in protein bands between 20 and 30 kDa and above 60 kDa, indicating protein degradation during the period. Proteolytic activity was detected in the albumin fraction of the seeds, which increased up to the fourth and then decreased up to the sixth day, when isolated chickpea total globulin and casein were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chickpea germination; Protein fractions; Major globulin; Protease activity; In vitro digestibility.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000400029
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Nutritional characteristics and in vitro digestibility of silages from different corn cultivars harvested at two cutting heights R. Bras. Zootec.
Caetano,H.; Oliveira,M.D.S.; Freitas Júnior,J.E.; Rêgo,A.C.; Carvalho,M.V.; Rennó,F.P..
The objective of this study was to evaluate the nutritional traits and in vitro digestibility of silages from different corn cultivars harvested at two cutting heights. It was evaluated 11 cultivars (Dina 766, Dina 657, Dina 1000, P 3021, P 3041, C 805, C 333, AG 5011, FO 01, Dina co 9621 and BR 205) harvest 5 cm above ground (low) and 5 cm below the intersection of the first ear (high). It was used a random block design (three blocks), arranged in a 11 × 2 factorial scheme. Silages from plants harvested at high cutting height presented average content of dry matter significantly superior to silages from plants harvested at low height. Cultivars FO 01, AG 5011, Dina co 9621 and Dina 766 presented greater content of crude protein than cultivars C 805, P...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chemical composition; Corn cultivars; In vitro digestibility; Silage.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000400002
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Pepsin-Cellulase Digestibility of Pasture Silages: Effects of Pasture Type, Maturity Stage, and Variations in the Enzymatic Method Chilean J. Agric. Res.
Barchiesi-Ferrari,Claudia; Alomar,Daniel; Miranda,Horacio.
Enzymatic in vitro digestibility has been studied as a method to predict energy values of forages for ruminants, although results have been affected by type of forage and methodological details of the technique. This work was performed to evaluate the effects of cellulase concentration (0.75, 1.0 and 6.25 g L-1), incubation time (24 or 48 h) and type of final washing of the residue (water or acetone) on the in vitro digestibility of the dry matter (DMD), organic matter (OMD) and content of digestible organic matter in the DM (D value) of silages made at three maturity stages from three types of pastures: a) permanent pasture (Dactylis glomerata, Lolium perenne, Bromus catharticus Vahl var. catharticus, Trifolium repens and Holcus lanatus); b) Italian...
Tipo: Journal article Palavras-chave: Cellulase incubation; Enzymatic digestibility; In vitro digestibility.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000200010
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Phosphorylated protein concentrate pumpkin seed (Cucurbita moschata): optimization by response surface methodology and nutritional characterization Ciência Rural
Lovatto,Naglezi de Menezes; Loureiro,Bruno Bianchi; Bender,Ana Betine Beutinger; Loureiro,Cristiane Bianchi; Goulart,Fernanda Rodrigues; Speroni,Caroline Sefrin; Macagnan,Fernanda Teixeira; Piana,Mariana; Silva,Leila Picolli da.
ABSTRACT: Response surface methodology was used to determine optimum conditions for extraction of protein from pumpkin seed meal. A central composite rotational design (CCRD) including independent variables such, pH and sodium trimetaphosphate (STMP) content was used. Maximum yield was obtained at a central point of CCRD, with STMP content and pH of 4% and 4.5, respectively. These conditions resulted in protein yield of 50.04 g of soluble protein from extract/100 g pumpkin seed. The optimization of pH and STMP content allowed obtaining a product with a high protein concentration (62.56 g 100 g-1), digestibility (62.03 g 100 g-1) and concentration of essential amino acids (27.26 g 100 g-1). Regarding the polyphenols concentration, phosphorylated protein...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Central composite rotational design; In vitro digestibility; New ingredient.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200751
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Production of alfalfa hay under different drying methods R. Bras. Zootec.
Neres,Marcela Abbado; Castagnara,Deise Dalazen; Mesquita,Eduardo Eustáquio; Zambom,Maximilliane Alavarze; Souza,Leiliane Cristine de; Oliveira,Paulo Sérgio Rabello de; Jobim,Clóves Cabreira.
This trial aimed at determining the dehydration curve at nine times (0, 3, 6, 9, 24, 27, 30, 33 and 46 hours after cuting of the plants); the leaf/stem ratio and number of leaves per stem in alfalfa submitted to four managements during the drying process. It was also evaluated the bromatologic composition and in vitro digestibility of the dry matter and cell wall of alfalfa hay before storage. The drying methods applied for alfalfa were the following: drying in the field until 50% DM and drying conclusion in an airy shed; drying in the field without turning; drying with only one turning and drying with two turnings. The experimental design was completely randomized with four drying methods and five replications. Leaf dehydration was faster when compared to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drying in field; Haymaking; In vitro digestibility.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000800008
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Protein co-products and by-products of the biodiesel industry for ruminants feeding R. Bras. Zootec.
Carrera,Ricardo Andrés Botero; Veloso,Cristina Mattos; Knupp,Leonardo Sidney; Souza Júnior,Alcy Heleno de; Detmann,Edenio; Lana,Rogério de Paula.
The objective of the experiment was to classify 20 protein co-products and by-products of the biodiesel industry with potential to use in ruminant feeding. The meals evaluated were: cottonseed, canudo-de-pito, crambe, sunflower, castor-oil seeds detoxified with calcium, non-detoxified castor-oil seeds and soybean; and the cakes were: cottonseed, peanut, babassu, crambe, palm oil, sunflower, licuri, macauba seeds, non-detoxified castor-oil seeds, turnip and jatropha. The samples were quantified to determine dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), neutral detergent fiber corrected for ash and protein (NDFap), non-fiber carbohydrates (NFC), acid detergent fiber corrected for ash and protein (ADFap), lignin, cutin and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromatology; Chemical composition; Digestive traits; In vitro digestibility.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000500018
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Study of the proteins in the defatted flour and protein concentrate of baru nuts (Dipteryx alata Vog) Ciênc. Tecnol. Aliment.
Guimarães,Rita de Cássia Avellaneda; Favaro,Simone Palma; Viana,Antonio Camilo Arguelho; Braga Neto,José Antônio; Neves,Valdir Augusto; Honer,Michael Robin.
Baru (Dipteryx alata Vog.) is an abundant legume in the Brazilian Savanna. Its nuts can be exploited sustainably using its protein and lipid fractions. This study aimed to analyze the proteins of the nuts present in the defatted flour and protein concentrate in terms of their functional properties, the profile of their fractions, and the in vitro digestibility. The flour was defatted with hexane and extracted at the pH of higher protein solubility to obtain the protein concentrate. The electrophoretic profile of the protein fractions was evaluated in SDS-PAGE gel. The functional properties of the proteins indicate the possibility of their use in various foods, like soybeans providing water absorption capacity, oil absorption capacity, emulsifying...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dipteryx alata; Protein fractions; Functional properties; Solubility; In vitro digestibility.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000300007
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Testing Protein Digestibility in Red Grouper Epinephelus Morio using In Vitro and In Vivo Methods ArchiMer
Silva, A. F.; Escalante, Karla; Alvarez-gonzalez, C. A.; Guerrero-olazaran, M.; Cuzon, Gerard; Gaxiola, Gabriela.
The digestibility of pre-selected ingredients was evaluated for Epinephelus morio using pH-stat and multi-enzyme extract (stomach, intestine, and ceca). The degree of hydrolysis (DH) was tested with acid and alkaline proteases using crustacean meals, squid meal, fish protein concentrate (FPC), fish meal, dried whey, as well as canola paste (w/or w/o phytase), soybean meal (w/or w/o phytase), soyprotein concentrate (SPC), and wheat gluten. SPC produced DH values between 3.6-4.1%, obtained from the three different parts of the digestive tract. Canola paste produced high DH in the stomach by adding phytase to 0.8 and 2% feed. This process was repeated under alkaline conditions for DH, with 0.6-2.6% in the pyloric ceca, and 1-2.2% in the intestine. The DH from...
Tipo: Text Palavras-chave: ADC; In vitro digestibility; Enzymes; Fish; Feedstuffs.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00248/35930/80189.pdf
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The importance of heat against antinutritional factors from Chenopodium quinoa seeds Ciênc. Tecnol. Aliment.
Silva,José Antonio da; Pompeu,Dávia Guimarães; Costa,Olavo Flores da; Gonçalves,Daniel Bonoto; Spehar,Carlos Roberto; Marangoni,Sérgio; Granjeiro,Paulo Afonso.
Chenopodium quinoa seeds have high protein content. The nutritional value of quinoa is superior compared with traditional cereals. Its essential amino acid composition is considered next to the ideal, and its quality matches that of milk proteins. In this study, the seed storage proteins from Chenopodium quinoa were extracted, fractionated, partially purified, and characterized. The structural characterization was performed by Tricine-SDS-PAGE and two-dimensional electrophoresis, and it confirmed the presence of proteins of molecular weight of 30 and 7kDa, probably corresponding to lectins and trypsin inhibitors, respectively. The functional characterization of these proteins evidenced their activity as antinutritional factors due to their in vitro...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chenopodium quinoa; Seeds; Lectins; Protease inhibitor; In vitro digestibility.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000100074
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Utilization of a novel feedstuff: effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (Robinia pseudoacacia) silage Ciência Rural
Dong,Zhihao; Li,Junfeng; Chen,Lei; Wang,Siran; Shao,Tao.
ABSTRACT: This study was conducted to evaluate the effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (TBL). The TBL leaves silage was either untreated (control) or treated with 1 × 106 cfu/g FM Lactobacillus plantarum (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and Lactobacillus plantarum (L+G), or a combination of 3% molasses and Lactobacillus plantarum (L+M). Fermentation quality, chemical composition and nutrient digestibility were then analyzed. Ethanol and acetic acid concentrations were the dominant fermentation products in all silages except L+M silage. The L, G and L+G treatments failed to influence the fermentation. The M treatment increased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Additives; Fermentation quality; In vitro digestibility; Chemical composition; Tetraploid black locust..
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700201
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