Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Rev. Bras. Ciênc. Avic. (4) Arq. Bras. Med. Vet. Zootec. (2) BJMBR (1) Autor Abreu,JT (1) Almeida,M.R. (1) Assao,V.S. (1) Augusto-Pinto,A. (1) Brentano,L (1) Bressan,G.C. (1) Campos,AB (1) Cardoso,T.C. (1) Cardoso,TC (1) Caron,L. (1) Cook,JKA (1) Da-Silva,SEL (1) Esteves,P.A. (1) Fernandez,S (1) Ferreira,HL (1) Fietto,J.L.R. (1) Finger,P.F. (1) Flatschart,RB (1) Folgueras-Flatschart,AV (1) Franco,GR (1) Mais... Palavra-chave Infectious bronchitis virus (7) Adenovirus vector (1) Broilers (1) Characterization (1) Control (1) Detection (1) Differentiation (1) ELISA (1) Genotyping (1) History (1) Humoral response (1) Immune responses (1) Isolation (1) Liquid phase blocking ELISA (1) Nucleocapsid (1) Nucleocapsid protein (1) Phylogenetic tree (1) Post vaccinal response (1) Semi-nested RT-PCR (1) Spike glycoprotein (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Info:eu-repo/semantics/other (3) Ano 2019 (1) 2018 (1) 2010 (2) 2005 (1) 2001 (1) 1999 (1) Mais... País Brazil (7) Idioma Inglês (7)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 7 Primeira ... 1 ... Última A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus BJMBR Cardoso,T.C.; Mouro-Sousa,R.L.; Oliveira,C.; Stringhini,G.; Augusto-Pinto,A.. A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested. The respective serum titers obtained in the serum neutralization test (SNT) were compared with those obtained in the LPB-ELISA. There was a high correlation (r2 = 0.8926) between the two tests. The LPB-ELISA represents a single test suitable for the rapid detection of antibodies against bronchitis virus in... Tipo: Info:eu-repo/semantics/other Palavras-chave: Liquid phase blocking ELISA; Humoral response; Infectious bronchitis virus. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600010 Comparative Study of Three Different ELISA to Measure the Antibodies Against Infectious Bronchitis Virus in Vaccinated and Unvaccinated Broilers Rev. Bras. Ciênc. Avic. Cardoso,TC; Oliveira,C; Da-Silva,SEL; Ferreira,HL; Pinto,AA. Broilers were spray-vaccinated (n=150) with H120 serotype at one-day-old, challenged after 28 days with M41 IBV serotype and after bled at day 28, 34 and 46 after challenged. The respective sera were tested by the indirect ELISA (I-ELISA), sandwich ELISA (S-ELISA), liquid phase blocking ELISA (LPB-ELISA) and the standard serum neutralization test (SNT). For this purpose, a total of 300 sera samples, 150 from non vaccinated and 150 from vaccinated broilers were titrated by all the serological methods and the correlation coefficients were determined. The correlation values (r) between LPB-ELISA x SNT, S-ELISA x SNT, I-ELISA x SNT found werer = 0.98, r = 0.79, and r = 0.74, respectively. Nevertheless, the r between LPB-ELISA x S-ELISA, LPB-ELISA x I-ELISA and... Tipo: Info:eu-repo/semantics/article Palavras-chave: ELISA; Post vaccinal response; Infectious bronchitis virus; Broilers. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2001000100010 Construction and characterization of a recombinant vaccine encoding the nucleocapsid protein gene of avian infectious bronchitis virus Arq. Bras. Med. Vet. Zootec. Ritterbusch,G.A.; Esteves,P.A.; Trevisol,I.M.; Okino,C.H.; Jaenisch,F.R.F.; Morés,M.A.Z.; Caron,L.; Silva,A.D.; Finger,P.F.; Hübner,S.O.. RESUMO A vacinação é a forma mais utilizada para prevenir a bronquite infecciosa causada pelo vírus da bronquite infecciosa das galinhas (IBV). Contudo, as vacinas convencionais são incapazes de diferenciar aves infectadas de vacinadas. No presente trabalho foi construído, caracterizado, e avaliado como candidato vacinal, um adenovírus recombinante expressando o gene N do IBV. O gene N foi clonado em um adenovírus humano tipo 5 defectivo e transfectado para as células HEK-293A para gerar rAd5_N. Após o vetor ser obtido como esperado e a confirmação da expressão da proteína N em HEK-293ª, foi realizada inoculação pela via oculo-nasal na dose de 10 7 TCID 50 /0,1mL para imunização de galinhas livres de patógenos específicos (SPF). A resposta imunológica do... Tipo: Info:eu-repo/semantics/other Palavras-chave: Infectious bronchitis virus; Nucleocapsid protein; Adenovirus vector; Immune responses. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000401428 Infectious bronchitis virus: detection and vaccine Strain differentiation by semi-nested RT-PCR Rev. Bras. Ciênc. Avic. Okino,CH; Montassier,MFSM; Givisiez,PEN; Furuyama,CRAG; Brentano,L; Montassier,HJ. A semi-nested reverse transcription-polymerase chain reaction (Semi-N-RT-PCR) was developed and used to detect the S glycoprotein gene of infectious bronchitis virus (IBV) strains and to discriminate H120 vaccine strain from other strains. Viral RNA was extracted from the allantoic fluid of chicken embryos and from tissues of chickens experimentally infected with different strains of IBV. Amplification and identification of the viral RNA was performed using two sets of primers complementary to a region of the S glycoprotein gene in the Semi-N-RT-PCR assay. The pair of primers used in the first PCR consisted of universal oligonucleotides flanking a more variable region of S1-S2 gene. The second primer pair was used in the Semi-N-RT-PCR and was comprised of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Detection; Differentiation; Infectious bronchitis virus; Semi-nested RT-PCR; Vaccine strain. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000100010 Infectious bronchitis virus in Asia, Africa, Australia and Latin America: history, current situation and control measures Rev. Bras. Ciênc. Avic. Wit,JJ (Sjaak) de; Cook,JKA; van der Heijden,HMJF. Tipo: Info:eu-repo/semantics/article Palavras-chave: Control; Infectious bronchitis virus; History; Variants. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200004 Isolation and genetic stability of an infectious bronchitis virus strain (IBV) Arq. Bras. Med. Vet. Zootec. Pereira,C.G.; Saraiva,G.L.; Santos,M.R.; Assao,V.S.; Fietto,J.L.R.; Bressan,G.C.; Almeida,M.R.; Moreira,M.A.S.; Silva Júnior,A.. RESUMO O vírus da bronquite infecciosa (IBV) é um importante patógeno respiratório presente na avicultura comercial e tem provocado grandes perdas econômicas em todo o mundo. A vacinação é realizada pela indústria produtora de aves, mas continuam surgindo novos sorotipos e variações antigênicas, dificultando o controle de IBV. Nós realizamos uma caracterização molecular de uma cepa de IBV obtida diretamente de tecidos e comparamos com a mesma cepa que havia sido passada três vezes em ovo embrionado. Nós mostramos uma variação significante na sequência viral depois de ter sido isolada em ovo embrionado. Tipo: Info:eu-repo/semantics/other Palavras-chave: Infectious bronchitis virus; Isolation; Characterization; Stability. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401333 Molecular studies of the Brazilian infectious bronchitis virus isolates Rev. Bras. Ciênc. Avic. Abreu,JT; Mourão,MM; Santos,CE; Veloso,CJM; Resende,JS; Flatschart,RB; Folgueras-Flatschart,AV; Júnior,SN; Santoro,MM; Mendes,ACR; Franco,GR; Silva,A; Campos,AB; Fernandez,S. Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Infectious bronchitis virus; Genotyping; Spike glycoprotein; Nucleocapsid; Phylogenetic tree. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200005 Registros recuperados: 7 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco