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16s-23S rDNA: polymorphisms and their use for detection and identification of Xylella fastidiosa strains BJM
Martinati,Juliana Camargo; Pacheco,Flávia Tereza Hansen; Miranda,Vitor Fernandes Oliveira de; Tsai,Siu Mui.
Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S-23S rDNA (coding for a small subunit ribosomal RNA) spacer region (ITS). Current methods for sequencing the ITS region yields partial sequences that do not contribute with significant information. According to this fact, new primers have been designed in order to obtain a complete sequence and facilitate the sequencing. The complete 16S-23S sequences from 08 strains were amplified through PCR using the primers designed in our lab. The 16S-23S sequences obtained were compared with 52 others sequences entries in GenBank database. The results revealed a higher level of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; 16S-23S rDNA; Intergenic spacer; Identification; Detection.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100033
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Sequence analysis of the rDNA intergenic spacer of Metarhizium strains isolated in Brazil Genet. Mol. Biol.
Yanaka-Schäfer,Fabiana Y.; Dall'Onder,Leonara P.; Panichi,Mariana C.; Mendes,Roberta G.; Fagundes,Nelson J.R.; Bandinelli,Josiane B.; Bogo,Maurício R..
To assess the extent of genetic variability of rDNA intergenic spacer (IGS) in Metarhizium sp., 34 strains (27 isolated in Brazil) were sequenced and analyzed together with an additional 20 Metarhizium anisopliae var. anisopliae sequences retrieved from GenBank. Overall, the global nucleotide diversity for the region under study was of 0.090, while for the Brazilian isolates it was only 0.016. Phylogenetic analyses showed four well-supported groups (A, B, C, and D), one of which (D) has not been previously identified. All but one of the Brazilian strains cluster in this novel D phylogroup, suggesting that the genetic variation found in Brazil is a subset of the worldwide M. anisopiliae var. anisopliae variation.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Intergenic spacer; Metarhizium; RDNA.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100020
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