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Aspergillus niveus Blochwitz 4128URM: new source for inulinase production BABT
Souza-Motta,Cristina Maria de; Cavalcanti,Maria Auxiliadora de Queiroz; Porto,Ana Lúcia Figueiredo; Moreira,Keila Aparecida; Lima Filho,José Luiz de.
Aspergillus niveus Blochwitz 4128 URM isolated from sunflower rhizosphere demonstrated a new source of inulinase. The enzyme was produced in culture medium containing inulin as substrate in the concentrations: 10, 15 and 20g L-1. Maximum enzyme activity was obtained in medium containing 20g L-1 inulin. The enzyme was partially purified using ammonium sulphate precipitation, followed by ion charge (DE-32) and molecular exclusion (Sephadex) chromatography. The results showed the optimal pH and temperature of inulinase from crude extract were 4.0 and 4.8 and 45ºC, respectively. The enzyme was purified 34.65 fold with yield of 53.63%. A. niveus 4128URM can be used in the inulinase production with use in the food industries.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niveus; Rhizosphere; Inulinase; Inulin; Purification.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300003
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Characterization of goat milk and potentially symbiotic non-fat yogurt Ciênc. Tecnol. Aliment.
Paz,Noelia Fernanda; Oliveira,Enzo Gonçalvez de; Kairuz,Martha Susana Nuñez de; Ramón,Adriana Noemí.
Combining prebiotics and probiotic microorganisms improve quality in the formulation of foods. In this paper, the characteristics of goat milk and symbiotic yogurt were studied. Raw goat milk was analyzed and the skimming process was optimized. For the formulation of a potentially non-fat symbiotic yogurt made with skimmed goat milk, inulin, gelatin, sugar, and Streptococcus salivarius subsp. thermophilus, Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus casei subsp. rhamnoshus. Chemical characteristics, acceptability, and viability of lactic acid bacteria and probiotic culture were assessed. The protein and fat content of the raw milk was 2.90 and 3.56 g/100 mL, respectively. The optimum skimming process was obtained at 9,800 rpm and 4 °C for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Goat milk; Yogurt; Symbiotic; L. casei; Inulin.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000300029
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Characterization of powder from the permeate of yacon extract by ultrafiltration and dehydrated by spray drying Ciência e Agrotecnologia
Brites,Marcela Lazzare; Meira,Stela Meister; Brandelli,Adriano; Noreña,Caciano Zapata.
ABSTRACT Yacon root is a functional food which contains antioxidants and prebiotics compounds. This study aimed to evaluate the physical, chemical and prebiotic characteristics of a yacon extract powder obtained by ultrafiltration (UF) with membranes of 10 and 30 kDa and encapsulation of the resulting permeate by spray drying. Drying air temperatures of 140 and 160 ºC and concentrations of gum arabic of 10 and 15% were tested. The samples had solubility values ​​greater than 90% while the hygroscopicity decreased with increasing gum concentration and drying temperature. Electron microscopy showed a strong tendency to agglomeration of smaller particles around the larger ones, mainly at a temperature of 140 ºC. Regarding color, the parameter L* showed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Encapsulation; Inulin; Prebiotic activity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000500585
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Comparison of rapid sensory characterization methodologies for the development of functional yogurts. Repositório Alice
CADENA, R. S.; CAIMI, D.; JAUNARENA, I.; LORENZO, I.; VIDAL, L.; ARES, G.; DELIZA, R.; GIMENÉZ, A..
bitstream/item/117336/1/1-s2.0-S0963996914005043-main.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Check-all-that-apply-questions; Projective mapping; Polarized sensory positioning; Probióticos; Prebiótico; Iogurte; Inulin; Prebiotics; Probiotics.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1006195
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Desenvolvimento de patê da pescada bicuda (Sphyraena tome, Fowler, 1903) com propriedades funcionais para diversificação e agregação de valor. Repositório Alice
ESTANECH, A. F. da C..
O pescado é um alimento altamente nutritivo fonte de proteínas de alto valor biológico variando de 15% a 25%, completo em aminoácidos essenciais, nutrientes fornecidos exclusivamente pela dieta, apresentando também excelentes valores para digestibilidade, além de apresentar baixo teor de gordura saturada, baixo valor calórico e a presença de micronutrientes importantes. O crescimento acelerado da população mundial tem refletido sobre a oferta de pescado como alimento, aumentando assim, a sua demanda e causando um preocupante desequilíbrio entre a oferta e a demanda, que tem sido mundialmente discutido. A sobre exploração das espécies mais almejadas tem contribuído para este desequilíbrio havendo assim, a necessidade da utilização das espécies subutilizadas...
Tipo: Tese/dissertação (ALICE) Palavras-chave: Patê; Inulina; Peixe; Produto Derivado de Pescado; Produto de Origem Animal; Processamento; Tecnologia de Alimento; Fish; Animal products; Food processing; Food technology; Inulin.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100215
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Development of Mangalarga Marchador suckling foals supplemented with fructooligosaccharides R. Bras. Zootec.
Dias,Ana Carla Chaves; Cabral,Grasiele Coelho; Ferreira e Silva,Camila; Ferreira,Marcelle Christine Nascimento; Franco,Tatiana Werneck; Moura,Raiane Araújo; Silva,Vinícius Pimentel; Almeida,Fernando Queiroz de.
ABSTRACT The objective of this study was to evaluate the effect of prebiotic fructooligosaccharide (FOS) supplementation on the development of Mangalarga Marchador suckling foals. Sixteen Mangalarga Marchador foals, from birth to 75 days of age, were evaluated in a completely randomized design, with two treatments (experimental group and control group) with eight repetitions (animals). The foals were evaluated through body weight, morphometric, and circumferential measures. The daily, weekly, and cumulative weight gains were recorded and the morphometric measures were: withers height, croup height, body length, mid-back height, mid-back width, and thorax, shin, knee, and forearm circumferences. The mean body weight was 32.3 and 77.9 kg at birth and 75 days...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Body weight; Equines; Inulin; Morphometric measures; Performance; Prebiotic.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000900747
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Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana L. BJM
De La Torre-Ruiz,Neyser; Ruiz-Valdiviezo,Víctor Manuel; Rincón-Molina,Clara Ivette; Rodríguez-Mendiola,Martha; Arias-Castro,Carlos; Gutiérrez-Miceli,Federico Antonio; Palomeque-Dominguez,Héctor; Rincón-Rosales,Reiner.
ABSTRACT The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA), solubilize phosphate, and had nitrogenase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agave; Inoculation; Bacteria; Inulin; 16S rRNA.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300587
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Encapsulation of Lactobacillus acidophilus and different prebiotic agents by external ionic gelation followed by freeze-drying Ciência Rural
Poletto,Gabriela; Fonseca,Bruna de Souza; Raddatz,Greice Carine; Wagner,Roger; Lopes,Eduardo Jacob; Barin,Juliano Smanioto; Flores,Erico Marlon de Moraes; Menezes,Cristiano Ragagnin de.
ABSTRACT: The aim of this study was to investigate the influence of the addition of prebiotics rice bran, inulin and hi-maize, on the survival of Lactobacillus acidophilus in alginate microparticles obtained by external ionic gelation followed by freeze-drying. The microparticles size ranged from 127.5μm to 234.6μm. Microparticles added from the different prebiotics demonstrated an increase in the protection of the microorganism, which presented greater viability against the gastrointestinal simulation. As for storage under different conditions, rice bran treatment at 25ºC kept probiotics viable for 30 days. Under storage conditions -18°C and 7°C, treatments containing prebiotics hi-maize and rice bran maintained viable probiotic microorganisms for a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microencapsulation; Probiotics; Rice bran; Inulin; Hi-maize.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200751
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Enhancing inulinase yield by irradiation mutation associated with optimization of culture conditions BJM
Gou,Yafeng; Li,Jianhua; Zhu,Junbao; Xu,Wanyun; Gao,Jianfeng.
A new inulinase-producing strain was isolated from rhizosphere soils of Jerusalem artichoke collected from Shihezi (Xinjiang, China) using Jerusalem artichoke power (JAP) as sole carbon source. It was identified as an Aspergillus niger strain by analysis of 16S rRNA. To improve inulinase production, this fungus was subjected to mutagenesis induced by 60Co γ-irradiation. A genetically stable mutant (designated E12) was obtained and it showed 2.7-fold higher inulinase activity (128 U/mL) than the parental strain in the supernatant of a submerged culture. Sequential methodology was used to optimize the inulinase production of stain E12. A screening trial was first performed using Plackett-Burman design and variables with statistically significant effects on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulin; Ethanol fermentation; Inulinase; Central composite design; Response surface methodology.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300911
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Formulation of a peach ice cream as potential symbiotic food Ciênc. Tecnol. Aliment.
VILLALVA,Fernando Josué; CRAVERO BRUNERI,Andrea Paula; VINDEROLA,Gabriel; GONÇALVEZ DE OLIVEIRA,Enzo; PAZ,Noelia Fernanda; RAMÓN,Adriana Noemí.
Abstract Today’s population increasingly demands and consumes healthy products. For this reason, the food industry has been developing and marketing food with added bioactive components. The aim of this work was to formulate a peach ice cream reduced in calories with an added probiotic (Bifidobacterium lactis Bb-12) and prebiotics (inulin), and to evaluate its sensory quality and acceptability as potential symbiotic food. The moisture content was 76.47%; 7.14% protein; 0.15% fat; 6.37%; carbohydrates; 9.87% inulin; 1.22% ash; 0.201% calcium, 0.155% phosphorus and 0.168% sodium. On the first and 21th day of storage counts of B. lactis Bb – 12 was 4 x 108 CFU/mL and 1.5 x 107 CFU/mL, respectively. It was possible to formulate a peach ice cream reduced in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ice cream; Inulin; Probiotic; Prebiotic; Symbiotic.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000300456
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Fructan degradation and hydrolytic activity in tuberous roots of Viguiera discolor Baker (Asteraceae), a herbaceous species from the cerrado Rev. Bras. Bot.
Degasperi,Maria I.; Itaya,Nair M.; Buckeridge,Marcos S.; Figueiredo-Ribeiro,Rita De Cássia L..
Fructans of the inulin type are the major reserve carbohydrates in tuberous roots of Viguiera discolor, a perennial herb native to the cerrado. Changes in molecular mass of the polymer, followed by releasing free fructose suggested that hydrolysis could be related to the sprouting of the buds after the dormant period, when aerial parts of the plant are naturally absent. Excision of aerial parts resulted in the increase of fructan 1-exohydrolase (1-FEH) activity in tuberous roots after sprouting. 1-FEH was partially purified from this material by binding to ConA-Sepharose and the highest activity was detected at pH 5.4 and between 20 and 40 °C. Values of Km for V. discolor inulin could not be determined since no saturation was observed up to 10%. The study...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Asteraceae; Cerrado; Fructan 1-exohydrolase; Inulin.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042003000100003
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Fructan dynamics in the underground organs of Chresta exsucca (Asteraceae), a dry season flowering species Acta Botanica
Santos,Camila Silva dos; Abraão,Cinara Ferreira; Moraes,Moemy Gomes de.
ABSTRACT Climatic seasonality has an influence on the phenology of native Cerrado plants. Herbs and subshrubs tend to flower in the rainy season, although some species of these habits flower in the dry season. Reserve carbohydrates, stored in the underground organs, are used to support phases of high energy-demand, but also may protect plants from damage during periods of environmental limitation. In this study we evaluated variation in fructan levels in the underground organs of field-grown plants of Chresta exsucca among different phenological phases. Chresta exsucca flowers in the dry season and possesses a diffuse underground system, which stores inulin-type fructans. Resprouting was continual during the sampling period. Oligosaccharide content was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; Drought; Inulin; Non-structural carbohydrate; Phenology.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100070
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Fructan production in Vernonia herbacea (Vell.) Rusby is related to adequate nitrogen supply and period of cultivation Rev. Bras. Bot.
Cuzzuol,Geraldo R. F.; Carvalho,Maria Angela M.; Zaidan,Lilian B. P..
Previous studies showed that plants of Vernonia herbacea grown for one year under a limited nitrogen supply presented reduced growth and higher fructan content than plants treated with sufficient nitrogen supply. However, the total fructan production was similar in both plant groups due to the higher biomass of the underground reserve organ in nitrogen-sufficient (N-sufficient) plants. In the present study we aimed to evaluate if a stress growing condition under nitrogen-limited (N-limited) supply, following cultivation under N-sufficient supply would have a positive effect on fructan production. Plants cultivated during one year under N-sufficient supply (10.7 mmol L-1 N-NO3-) were separated in two groups. During the following six months, one group...
Tipo: Info:eu-repo/semantics/article Palavras-chave: " cerrado" vegetation; Inulin; Nutritional stress; Plant growth; Underground reserve organ.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042008000100010
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Fructans and water suppression on intact and fragmented rhizophores of Vernonia herbacea BABT
Dias-Tagliacozzo,Gláucia M.; Itaya,Nair M.; Carvalho,M. Angela Machado de; Figueiredo-Ribeiro,Rita de Cássia L.; Dietrich,Sonia M. C..
In order to evaluate the involvement of fructans in drought tolerance, experiments were conducted using intact plants and excised rhizophores of Vernonia herbacea. The water content in rhizophores of intact plants was maintained for 30 days when plants were watered every 7 and 15 days, whereas plants remained alive up to 60 days without water. Total fructan, oligo- to polysaccharides ratio and mean molecular mass of polysaccharides increased in these plants indicating depolymerization of median size molecules. In apical fragments of rhizophores kept dry the oligosaccharides increased in relation to polysaccharides one day after excision in treated tissues. This was reflected in the HPLC profile of the oligosaccharides in which the proportion of free...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulin; Environmental stresses; Drought; Fructan metabolism; Reserve organs; Asteraceae.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300005
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Inulin as a growth promoter in diets for rabbits R. Bras. Zootec.
Montiel,Rocío Salas; Acosta,Irene Torres; Delgado,Enrique Villarreal; Juárez-Silva,María Eugenia; Azaola,Alejandro; Romo,Fernando Pérez-Gil.
Sixty New Zealand rabbits aged 40 days were divided into 4 groups of 15 animals. The control group received a free diet of antibiotic growth promoter (AGP) and inulin. The second group was supplemented with 2.5 g/kg of inulin. The third was administered AGP with 0.1 g/kg of flavomycin. Finally, the fourth group received a 2.5 and 0.1 g/kg inulin/AGP diet. Body weight gain was higher in the control group. Rabbits supplemented with inulin had lower values ​​of triglycerides compared with the control and AGP groups, and their glucose level was significantly lower than those treated with AGP. Additionally, serum calcium and magnesium concentrations were higher than the other groups, particularly with regard to AGP. The bone content with regard to calcium,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bone; Caecal; Crypts; Diet; Inulin; Minerals; Mucosa.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013001200008
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Inulin Induces IL-10 Secretion and Increased FOXP3 Gene Expression in Human Peripheral Blood Mononuclear Cells BABT
Amarante,Marla Karine; Ariza,Carolina Batista; Oliveira,Carlos Eduardo Coral de; Gualtieri,Karina de Almeida; Oda,Julie Massayo Maeda; Watanabe,Maria Angelica Ehara.
ABSTRACT Inulin is an effective prebiotic and its potential in modulating systemic immunity have been proposed. A subpopulation of T cells, named T regulatory cells (Tregs), expressing the Forkhead boxP3 transcription factor are key mediators of peripheral tolerance and suppress undesirable immune responses. These Tregs can be induced by cytokine transforming growth factor beta (TGF-β) and interleukin 10 (IL-10). This work aimed to evaluate inulin effects on human peripheral blood mononuclear cells (PBMC) in vitro. PBMC were incubated with inulin, and the expression of TGF-(1, FOXP3 and IL-10 was analyzed. Increased supernatant IL-10 levels were observed in PBMC of inulin-treated group (p=0.03). Moreover, FOXP3 gene expression was 7.6 fold higher in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulin; FOXP3; TGF-β1; IL-10; PBMC.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100417
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Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1 BJM
Hao,Zhikui; Cai,Yujie; Liao,Xiangru; Zhang,Xiaoli; Fang,Zhiyou; Zhang,Dabing.
The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Medium optimization; Response surface method; Inulin; Chitinolyticbacter meiyuanensis SYBC-H1.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100019
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Performance of low-fat beef burger with added soluble and insoluble dietary fibers Ciênc. Tecnol. Aliment.
BIS-SOUZA,Camila Vespúcio; HENCK,Jenifer Mayara Monari; BARRETTO,Andrea Carla da Silva.
Abstract Inulin, fructooligosaccharide, oat fiber and wheat fiber were added at 3 and 6% level in low-fat beef burger and their performances were evaluated. The low-fat beef burgers were submitted to cooking losses, objective color, texture profile, TBARS value, pH, microbiological and sensory evaluations. FR3, FR6, IN3 e IN6 showed no difference from the control to yield. Hardness values increased in OF6, WF3 and WF6 and it was similar to control. WF6 was the lowest score for the overall acceptance. Higher yield and hardness were found with the addition of insoluble fibers at 6% level. The TBARS values were not influenced by the addition of dietary fibers in low-fat beef burger. Inulin at 6% (IN6) level showed the highest score for all the sensory...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dietary fiber; Acceptability; Fat substitute; TPA; Inulin.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000300522
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Prebiotic inulin: Useful dietary adjuncts to manipulate the livestock gut microflora BJM
Samanta,A.K.; Jayapal,Natasha; Senani,S.; Kolte,A.P.; Sridhar,Manpal.
In recent years, there has been a growing appreciation on the relevance of gastrointestinal microflora in both ruminants and non-ruminants owing to revelation of their role in several physiological functions including digestion, nutrient utilization, pathogen exclusion, gastrointestinal development, immunity system, gut gene expression and quality of animal products. The ban imposed on the use of antibiotics and hormones in feed has compelled animal researchers in finding an alternative which could overcome the issues of conventional feed additives. Though the concept of prebiotic was evolved keeping in mind the gastrointestinal flora of human beings, presently animal researchers are exploring the efficiency of prebiotic (inulin) for modulating the gut...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prebiotic; Inulin; Mechanism of action; Livestock.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100001
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Production and action pattern of inulinase from Aspergillus Niger-245: hydrolysis of inulin from several sources Rev. Microbiol.
Cruz,Vinícius D’Arcadia; Belote,Juliana Gisele; Belline,Márcia Zilioli; Cruz,Rubens.
A strain of Aspergillus niger isolated from soil samples showed great capacity to produce extracellular inulinase. Although the enzyme has been synthesized in presence of monosaccharides, sucrose and sugar cane molasse, the productivity was significantly higher (p<0.05) when the microorganism was inoculated in media formulated with dahlia extract and pure inulin, as carbon sources. With regard to the nitrogen source, the best results were obtained with casein and other sources of proteic nitrogen, comparatively to the mineral nitrogen. However, statistic significance (p<0.01) only was found between the productivity obtained in the medium prepared with casein and ammonium sulphate. The optimum pH of the purified enzyme for inulin hydrolysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulinase; Aspergillus niger; Fructose syrup; Inulin.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400013
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