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Aspergillus niveus Blochwitz 4128URM: new source for inulinase production BABT
Souza-Motta,Cristina Maria de; Cavalcanti,Maria Auxiliadora de Queiroz; Porto,Ana Lúcia Figueiredo; Moreira,Keila Aparecida; Lima Filho,José Luiz de.
Aspergillus niveus Blochwitz 4128 URM isolated from sunflower rhizosphere demonstrated a new source of inulinase. The enzyme was produced in culture medium containing inulin as substrate in the concentrations: 10, 15 and 20g L-1. Maximum enzyme activity was obtained in medium containing 20g L-1 inulin. The enzyme was partially purified using ammonium sulphate precipitation, followed by ion charge (DE-32) and molecular exclusion (Sephadex) chromatography. The results showed the optimal pH and temperature of inulinase from crude extract were 4.0 and 4.8 and 45ºC, respectively. The enzyme was purified 34.65 fold with yield of 53.63%. A. niveus 4128URM can be used in the inulinase production with use in the food industries.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niveus; Rhizosphere; Inulinase; Inulin; Purification.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300003
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Culture conditions for production of 2-1-β-D-Fructan-fructanohydrolase in solid culturing on chicory (Cichorium intybus) roots BABT
Pandey,Ashok; Kavita,Poomarathil; Selvakumar,Ponniah.
Investigations were carried out to optimize the culture conditions for the production of b-D-fructan-fructanohydrolase by an indigenously isolated bacterial culture of Staphylococcus sp. RRL8. Experiments were carried out in solid culturing using chicory roots (powdered) as the source of carbon, which was supplemented, with corn steep liquor and potassium dihydrogen phosphate. A number of process parameters, like period of cultivation, initial moisture content in the substrate and temperature of incubation were optimized. Maximum extra-cellular enzyme was produced when fermentation was carried out at 30ºC for 24 h using chicory roots with 60% initial moisture. Supplementation of the substrate with additional carbon source (except with sucrose) resulted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fructan-fructanohydrolase; Inulinase; Solid state fermentation; Staphylococcus sp.; Chicory.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200010
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Effect of extrinsic and intrinsic parameters on inulinase production by Aspergillus niger ATCC 20611 Electron. J. Biotechnol.
Dinarvand,Mojdeh; B. Ariff,Arbakariya; Moeini,Hassan; Masomian,Malihe; Mousavi,Seyed Sadegh; Nahavandi,Reza; Mustafa,Shuhaimi.
Background: Inulinase is a versatile enzyme from glycoside hydrolase family which targets the β-2, 1 linkage of fructopolymers. In the present study, the effect of medium composition and culture conditions on inulinase production by Aspergillus niger ATCC 20611 was investigated in shake-flasks. Results: The highest extracellular inulinase (3199 U/ ml) was obtained in the presence of 25% (w/v) sucrose, 0.5% (w/v) meat extract, 1.5% (w/v) NaNO3 and 2.5 mM (v/v) Zn2+, at initial pH of 6.5, temperature 35ºC and 6% (v/v) of spores suspension in the agitation speed of 100 rpm. Surfactants showed an inhibitory effect on enzyme production. The optimum temperature for inulinase activity was found to be 50ºC. TLC analysis showed the presence of...
Tipo: Journal article Palavras-chave: Aspergillus niger ATCC 20611; Culture optimization; Inulinase.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400005
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Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus BABT
Cazetta,Marcia Luciana; Monti,Rubens; Contiero,Jonas.
The present study was conducted to investigate the influence of initial sucrose concentration, pH and aeration rate on biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus in a stirred batch reactor. Maximum inulinase activity (15.29 UmL-1) was obtained at a sucrose concentration of 10 g L-1, pH 5.0 and aeration rate of 1 vvm. The 20 g L-1 sucrose concentration was suitable for cell growth; however, enzymatic activity at this concentration was inhibited due to catabolic repression. The increase in aeration rate caused a reduction in enzyme activity with no relevant biomass increase.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kluyveromyces marxianus var. bulgaricus; Sucrose; Inulinase; Biomass; Batch fermentation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300027
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Enhancing inulinase yield by irradiation mutation associated with optimization of culture conditions BJM
Gou,Yafeng; Li,Jianhua; Zhu,Junbao; Xu,Wanyun; Gao,Jianfeng.
A new inulinase-producing strain was isolated from rhizosphere soils of Jerusalem artichoke collected from Shihezi (Xinjiang, China) using Jerusalem artichoke power (JAP) as sole carbon source. It was identified as an Aspergillus niger strain by analysis of 16S rRNA. To improve inulinase production, this fungus was subjected to mutagenesis induced by 60Co γ-irradiation. A genetically stable mutant (designated E12) was obtained and it showed 2.7-fold higher inulinase activity (128 U/mL) than the parental strain in the supernatant of a submerged culture. Sequential methodology was used to optimize the inulinase production of stain E12. A screening trial was first performed using Plackett-Burman design and variables with statistically significant effects on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulin; Ethanol fermentation; Inulinase; Central composite design; Response surface methodology.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300911
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Identification and characterization of filamentous fungi isolated from the sunflower (Helianthus annus L.) rhizosphere according to their capacity to hydrolyse inulin BJM
Souza-Motta,Cristina Maria de; Cavalcanti,Maria Auxiliadora de Queiroz; Fernandes,Maria José dos Santos; Lima,Débora Maria Massa; Nascimento,João Paulo; Laranjeira,Delson.
Filamentous fungi able to hydrolyse inulin have been isolated from the rhizosphere of plants whose roots contain this polysaccharide. This study reports results concerning the isolation and identification of filamentous fungi from the soil used for sunflower cultivation and from the sunflower rhizosphere cultivated in field and in greenhouse. Fungi were evaluated according to their capacity to hydrolyse inulin and the variation in the diversity of these fungi during the plant's life cycle was also accessed. Forty-nine species of filamentous fungi were isolated. Penicillium and Aspergillus were the genera that presented higher number of species, nine and seven, respectively. At the end of the sunflower life cycle, cultivated both in field and in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Rhizosphere; Soil; Inulinase; Sunflower.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300018
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Imobilização da inulinase de Kluyveromyces marxianus para a hidrólise de extratos de Helianthus tuberosus L. Scientia Agricola
Gaspari,Jefferson Willians de; Gomes,Luiz Humberto; Tavares,Flavio Cesar Almeida.
Este trabalho estudou a imobilização da inulinase de Kluyveromyces marxianus em diferentes suportes, com a finalidade de promover a bioconversão da inulina de Helianthus tuberosus. A inulina de H. tuberosus foi extraída dos tubérculos, desproteinizada e concentrada a 25% de açúcares redutores totais (ART). A inulinase de K. marxianus foi concentrada em evaporador rotativo e imobilizada em quitina (com e sem glutaraldeído), alginato sódico (concentrações de 2 e 4%), pectina, membrana de diálise e sílica de porosidade controlada (SPC). Em quitina obteve-se taxas de imobilização de 73Unidades/g com glutaraldeído e 48 U/g sem glutaraldeído, mas a hidrólise foi baixa em ambos os tratamentos, o equivalente a 2,4% por hora. Em gel de alginato sódico, nas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Helianthus tuberosus; Enzima; Inulinase; Inulina.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000500015
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Partial characterization of an inulinase produced by Aspergillus japonicus URM5633 BABT
Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Porto,Tatiana Souza; Spier,Michele Rigon; Soccol,Carlos Ricardo; Porto,Ana Lúcia Figueiredo; Souza-Motta,Cristina Maria; Moreira,Keila Aparecida.
Enzymes obtained by fermentation processes offer a number of advantages and have been widely researched and used throughout the world. This study aimed to partially characterise an inulinase produced from palm and cassava peel. The enzyme was produced via the solid-state fermentation of Aspergillus japonicus URM5633. The optimal temperatures were 50ºC and 55ºC, and the optimal pH values were 5.2 and 3.4 for inulinase fermentatively produced from palm and cassava peel, respectively. The thermostability measurements for inulinase produced in palm showed that the relative activity remained below 100% until 30 minutes of stability for all temperatures, but reached 106.8% at a temperature of 50ºC after 60 minutes. Inulinase from the crude extract of cassava...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulinase; Characterisation; Solid-state fermentation; Aspergillus japonicus.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500005
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Production and action pattern of inulinase from Aspergillus Niger-245: hydrolysis of inulin from several sources Rev. Microbiol.
Cruz,Vinícius D’Arcadia; Belote,Juliana Gisele; Belline,Márcia Zilioli; Cruz,Rubens.
A strain of Aspergillus niger isolated from soil samples showed great capacity to produce extracellular inulinase. Although the enzyme has been synthesized in presence of monosaccharides, sucrose and sugar cane molasse, the productivity was significantly higher (p<0.05) when the microorganism was inoculated in media formulated with dahlia extract and pure inulin, as carbon sources. With regard to the nitrogen source, the best results were obtained with casein and other sources of proteic nitrogen, comparatively to the mineral nitrogen. However, statistic significance (p<0.01) only was found between the productivity obtained in the medium prepared with casein and ammonium sulphate. The optimum pH of the purified enzyme for inulin hydrolysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulinase; Aspergillus niger; Fructose syrup; Inulin.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400013
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Production, characterization and application of inulinase from fungal endophyte CCMB 328 Anais da ABC (AABC)
Nascimento,Diego S.; Valasques Junior,Gildomar; Fernandes,Pedro; Ribeiro,Geise C.A.; Lima,Danyo M.; Góes-Neto,Aristóteles; Oliveira,Rodrigo Q.; Figueiredo-Ribeiro,Rita de Cassia L.; Assis,Sandra A. de.
Inulinase (β-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulin; Inulinase; Yeasts; Semi-arid; Fungal endophyte.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200014
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Production of inulinase from Kluyveromyces marxianus using Dahlia tuber extract BJM
Jain,Sumat Chand; Jain,P.C.; Kango,Naveen.
Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest inulinase activity was observed with Dahlia extract (25.3 nkat mL-1) as carbon source. The enzyme activity was 1.4 folds higher than that observed in media containing pure chicory inulin (17.8 nkat mL-1). The yeast showed good growth on a simple medium containing dahlia extract (20% w/v) and yeast extract (2%w/v) as carbon and nitrogen source respectively, in 96 h. at 28°C and 120 rpm. Lowest inulinase yield (4.8 nkat mL-1) was seen in the medium containing glucose as C-source. Although varied inulinase levels were noticed on different C- sources, Inulinase: Sucrase (I/S) ratios were noticed to be similar. Among various protein sources...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulinase; Dahlia; Fructose; Kluyveromyces marxianus.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100007
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Purification and characterization of β-Fructosidase with inulinase activity from Aspergillus niger - 245 BABT
Cruz,Vinícius D'Arcadia; Belote,Juliana Gisele; Dorta,Claudia; Santos,Luíza Helena Oliveira dos; Andriolo,Cláudia Regina; Khenayfes,Marcelo de Oliveira; Cruz,Rubens.
Aspergillus niger - 245, a strain isolated from soil samples showed good β-fructosidase activity when inoculated in medium formulated with dahlia extract tubers. The enzyme was purified by precipitation in ammonium sulphate and percolated in DEAE-Sephadex A-50 and CM-cellulose columns, witch showed a single peack in all the purification steps, maintaining the I/S ratio between 0.32 to, 0.39. Optimum pH for inulinase activity (I) was between 4.0 - 4.5 and for invertase activity (S) between 2.5 and 5.0. The optimum temperature was 60O.C for both activities and no loss in activity was observed when it was maintained at this temperature for 30 min. The Km value was 1.44 and 5.0, respectively, for I and S and Vm value 10.48 and 30.55, respectively. The I...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulinase; Invertase; Β-fructosidase; Inulin; Fructose syrup; Aspergillus niger.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300003
Registros recuperados: 12
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