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| Registros recuperados: 18 | |
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| Mesquita,Alessandro Carlos; Oliveira,Luiz Edson Mota de; Mazzafera,Paulo; Delú-Filho,Nelson. |
| Rubber production is dependent on the availability of carbohydrates in the tissues engaged in latex synthesis. In this study three rubber tree clones were used to study the protein profile, the activities of invertase and sucrose synthase in the latex and anatomical characteristics of the bark, as well as to establish a possible relationship with latex production. The clones used in the study, RRIM 600, GT 1 and Fx2261, differed in latex productivity, from high to low in this order. Although present, few differences were observed in the latex protein profile obtained by denaturing electrophoresis. The activity of neutral invertase was higher than the acidic isoform, and both activities were higher in the most productive clones RRIM 600 and GT 1. The same... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hevea brasiliensis; Invertase; Lacticiferous vessels; Latex; Rubber; Sucrose synthase. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200002 |
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| ODA, Yuji; MIKUMO, Dai; TAJIMA, Kanako; YAMAUCHI, Hiroaki; 小田, 有二. |
| The yeast strain AK 46 was isolated from dough containing fermented cherry fruits and identified as Saccharomyces cerevisiae. The nucleotide sequence of the rDNA spacer region from this strain coincided with those from the yeasts used for the production of whisky, wine, and bread. In a comparison with six baking strains, strain AK 46 possessed less leavening ability in dough with and without addition of 5% sucrose (based on the weight of flour) but a high ability in sweet dough containing 30% sucrose. The activities of the two enzymes α-glucosidase and invertase, which are related to dough fermentation, were lower than those in the other strains. A baking test showed that strain AK 46 was applicable for breadmaking using the straight dough and sponge dough... |
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Palavras-chave: Baker's yeast; Leavening; Dough; SUC gene; Invertase. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2963 |
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| Fries,Daniela Deitos; Alves,José Donizeti; Delú Filho,Nelson; Magalhães,Paulo César; Goulart,Patrícia de Fátima Pereira; Magalhães,Marcelo Murad. |
| Objetivou-se avaliar o crescimento da plântula e o metabolismo de carboidratos associados ao aumento da tolerância à hipoxia exercido pela presença do cálcio no período de germinação e/ou alagamento de plântulas de milho com diferentes idades. O experimento foi desenvolvido na Universidade Federal de Lavras, MG, em 2002. Cariopses de milho da variedade 'Saracura' (BRS-4154) foram germinadas em água ou solução de CaCl2. Após dois e quatro dias, as plântulas foram submetidas ao alagamento em tubos de PVC com tampão (com ou sem CaCl2) por três dias, sendo então avaliadas a sobrevivência, massa seca e as características bioquímicas. O cálcio aumentou a sobrevivência ao alagamento de plântulas com quatro dias, entretanto, não influenciou naquelas com dois dias.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Invertase; Alfa-amilase; Cálcio; Hipoxia. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052007000100001 |
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| CARDOSO,Marisa H.; JACKIX,Marisa N.H.; MENEZES,Hilary C.; GONÇALVES,Elisabeth B.; MARQUES,Simone V.B.. |
| O efeito do tratamento em que se associou as enzimas comerciais: 0,03 % v/p de pectinase (Clarex) a 0,6 % v/p de invertase (Invertase-S) e 0,5 % p/p de glicose-isomerase (Taka-sweet) sobre purê de banana (Musa cavendishii), em condições amenas de hidrólise (40o C, 15 min.) foi observado e comparado com o efeito de outros três tratamentos enzimáticos: 0,03 % v/p de pectinase (Clarex); 0,03 % v/p de pectinase (Clarex) associada à 0,6 % v/p de invertase (Invertase-S); e 0,03 % v/p de pectinase (Sigma) associada a 0,03 % v/p de celulase (Sigma), visando determinar a qualidade representada por um conjunto de propriedades físicas, fisico-químicas, químicas, microbiológicas e sensoriais dos sucos de banana obtidos. Essas propriedades não diferiram... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pectinase; Celulase; Invertase; Glicose isomerase; Suco de banana; Qualidade. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20611998000300005 |
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| Reis,Angélica Pataro; Guimarães,Valéria Monteze; Ferreira,Joana Gasperazzo; Queiroz,José Humberto de; Oliveira,Maria Goreti Almeida; Falkoski,Daniel Luciano; Almeida,Maíra Nicolau de; Rezende,Sebastião Tavares de. |
| Two α -galactosidase (P1 and P2) and one invertase present in the culture of Aspergillus terreus grown on wheat straw for 168 h at 28ºC were partially purified by gel filtration and hydrophobic interaction chromatographies. Optimum pH and temperatures for P1, P2 and invertase preparations were 4.5-5.0, 5.5 and 4.0 and 60, 55 and 65ºC, respectively. The K M app for Ï -nitrophenyl-α -D-galactopyranoside were 1.32 mM and 0.72 mM for P1 and P2, respectively, while the K M app value for invertase, using sacarose as a substrate was 15.66 mM. Enzyme preparations P1 and P2 maintained their activities after pre-incubation for 3 h at 50ºC and invertase maintained about 90% after 6 h at 55 ºC. P1 and P2 presented different inhibition sensitivities by Ag+,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus terreus; Galactooligosaccharides; Α-galactosidase; Invertase; Soy molasses. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300029 |
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| FERREIRA,Marcela Vega; AVILA,Tariani Lemos de; KUHN,Claudio Rafael; TORALLES,Ricardo Peraça; ROMBALDI,César Valmor. |
| Abstract The identification of yeasts isolated from spoiled Jubileu peach puree using the API 20C AUX method and a commercial yeast as witness were studied. Subsequently, the yeast’s growth potential using two batch culture treatments were performed to evaluate number of colonies (N), reducing sugar concentration (RS), free-invertase (FI), and culture-invertase activity (CI). Stock cultures were maintained on potato dextrose agar (PDA) slants at 4 °C and pH 5 for later use for batch-culture (150 rpm) at 30°C for 24 h, then they were stored at 4 °C for subsequent invertase extraction. The FI extract was obtained using NaHCO3 as autolysis agent, and CI activity was determined on the supernatant after batch-cultured centrifugation. The activity was followed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spoiled peach puree; Invertase; Saccharomyces cerevisiae; Autolysis. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000400701 |
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| Silva,Aline Ramos da; Tomotani,Ester Junko; Vitolo,Michele. |
| Conversion of sucrose into fructose and gluconic acid using invertase, glucose oxidase and catalase was studied by discontinuous (sequential or simultaneous addition of the enzymes) and continuous (simultaneous addition of the enzymes in a 100 kDa-ultrafiltration membrane reactor) processes. The following parameters were varied: concentration of enzymes, initial concentration of substrates (sucrose and glucose), pH, temperature and feeding rate (for continuous process). The highest yield of conversion (100%) was attained through the discontinuous (batch) process carried out at pH 4.5 and 37 ºC by the sequential addition of invertase (14.3 U), glucose oxidase (10,000 U) and catalase (59,000 U). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sucrose/conversion; Fructose; Gluconic acid; Invertase; Glucose oxidase; Catalase. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200022 |
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| Alegre,Ana Cláudia Paiva; Polizeli,Maria de Lourdes Teixeira de Moraes; Terenzi,Héctor Francisco; Jorge,João Atílio; Guimarães,Luis Henrique Souza. |
| The filamentous fungus Aspergillus caespitosus was a good producer of intracellular and extracellular invertases under submerged (SbmF) or solid-state fermentation (SSF), using agroindustrial residues, such as wheat bran, as carbon source. The production of extracellular enzyme under SSF at 30ºC, for 72h, was enhanced using SR salt solution (1:1, w/v) to humidify the substrate. The extracellular activity under SSF using wheat bran was around 5.5-fold higher than that obtained in SbmF (Khanna medium) with the same carbon source. However, the production of enzyme with wheat bran plus oat meal was 2.2-fold higher than wheat bran isolated. The enzymatic production was affected by supplementation with nitrogen and phosphate sources. The addition of glucose in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus caespitosus; ²-D-fructofuranosidase; Invertase; Solid-state fermentation; Submerged fermentation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300025 |
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| Cruz,Vinícius D'Arcadia; Belote,Juliana Gisele; Dorta,Claudia; Santos,Luíza Helena Oliveira dos; Andriolo,Cláudia Regina; Khenayfes,Marcelo de Oliveira; Cruz,Rubens. |
| Aspergillus niger - 245, a strain isolated from soil samples showed good β-fructosidase activity when inoculated in medium formulated with dahlia extract tubers. The enzyme was purified by precipitation in ammonium sulphate and percolated in DEAE-Sephadex A-50 and CM-cellulose columns, witch showed a single peack in all the purification steps, maintaining the I/S ratio between 0.32 to, 0.39. Optimum pH for inulinase activity (I) was between 4.0 - 4.5 and for invertase activity (S) between 2.5 and 5.0. The optimum temperature was 60O.C for both activities and no loss in activity was observed when it was maintained at this temperature for 30 min. The Km value was 1.44 and 5.0, respectively, for I and S and Vm value 10.48 and 30.55, respectively. The I... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inulinase; Invertase; Β-fructosidase; Inulin; Fructose syrup; Aspergillus niger. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300003 |
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| Pataro,Carla; Guerra,Juliana B.; Gomes,Fátima C.O.; Neves,Maria J.; Pimentel,Patrícia F.; Rosa,Carlos A.. |
| Trehalose accumulation, invertase activity and physiological characteristics of 86 yeast isolates from short fermentative cycles during the production of cachaça in three artisanal distilleries of the State of Minas Gerais were studied. Among these isolates, 70% were able to grow at temperatures between 40 and 42ºC. Only Saccharomyces cerevisiae isolates were able to grow over 40ºC. Lower temperatures (<40ºC) favoured the growth of yeasts such as Candida parapsilosis-like, C. maltosa-like, Kloeckera japonica, S. exiguus and C. bombicola-like. The isolates from all three distilleries were ethanol tolerant, produced invertase, and accumulate trehalose in the presence of glucose. The strains isolated from distillery A presented more resistance to ethanol... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Saccharomyces cerevisiae; Cachaça; Trehalose; Invertase; Fermentation. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300003 |
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| DANTAS, B. F.; RIBEIRO, L. de S.. |
| O pólo de agricultura irrigada do Vale do Rio São Francisco a presenta um crescente aumento na produção de uvas para vinificação. Entre os vinhos finos ali produzidos, destaca-se aquele obtido da cv. Petite Syrah, que se adaptou bem às condições climáticas da região. Pouco se conhece, no entanto, sobre o metabolismo de carboidratos das videiras nessa região. O objetivo deste trabalho foi avaliar os teores de açúcarese de amido, bem como a atividade de invertases, durante dois ciclos de produção consecutivos. O experimento foi realizado na Embrapa Semi-Árido e na Vitivinícola Santa Maria, localizadas, respectivamente, em Petrolina e em Lagoa Grande - PE. Semanalmente, de janeiro a dezembro de 2003, foram coletadas folhas para análise dos teores de açúcares... |
| Tipo: Folhetos |
Palavras-chave: Videira; Variação; Vale do São Francisco; Carboidrato; Invertase; Uva. |
| Ano: 2005 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/156975 |
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| Melo,Renato Figueiredo; Oliveira,Luiz Edson Mota de; Mesquita,Alessandro Carlos; Delú Filho,Nelson. |
| Objetivou-se com este trabalho avaliar os efeitos das variações climáticas sazonais sobre a produção de látex, a partir de parâmetros relacionados ao fluxo e regeneração de látex e da caracterização bioquímica dos tecidos fonte e dreno, com base nas atividades das enzimas invertase neutra e sintase da sacarose (SuSy). Foram usados folíolos centrais de folhas completamente expandidas de clones de seringueira (RRIM 600, FX 2261 e GT 1) nos meses de dezembro de 2001 e julho de 2002, plantados no Setor de Fisiologia Vegetal da Universidade Federal de Lavras (UFLA). Os dados de produção foram obtidos nos respectivos meses, por meio de sangrias no sistema S ½ (D2/D3), além da utilização de borracha seca em estufa para análises minerais. Pelos dados de produção,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seringueira; Látex; Invertase; Sintase da sacarose. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000600015 |
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| Registros recuperados: 18 | |
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