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Registros recuperados: 63
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A novel acidophilic, thermophilic iron and sulfur-oxidizing archaeon isolated from a hot spring of tengchong, yunnan, China BJM
Ding,Jiannan; Zhang,Ruiyong; Yu,Yizun; Jin,Decai; Liang,Changli; Yi,Yang; Zhu,Wei; Xia,Jinlan.
A novel thermoacidophilic iron and sulfur-oxidizing archaeon, strain YN25, was isolated from an in situ enriched acid hot spring sample collected in Yunnan, China. Cells were irregular cocci, about 0.9-1.02 µm×1.0-1.31 µm in the medium containing elemental sulfur and 1.5-2.22 µm×1.8-2.54 µm in ferrous sulfate medium. The ranges of growth and pH were 50-85 (optimum 65) and pH 1.0-6.0 (optimum 1.5-2.5). The acidophile was able to grow heterotrophically on several organic substrates, including various monosaccharides, alcohols and amino acids, though the growth on single substrate required yeast extract as growth factor. Growth occurred under aerobic conditions or via anaerobic respiration using elemental sulfur as terminal electron acceptor. Results of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isolation; Identification; Acidianus manzaensis; Bioleaching.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200016
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A reassessment of the phytogeographic characterization of Temperate Pacific South America RChHN
SANTELICES,BERNABE; MENESES,ISABEL.
In 1980, a study of geographic distribution patterns and geographic affinities of benthic algae of temperate Pacific South America (5-55 ºS) provided a first characterization of these coasts. High endemism and limited floristic exchange with the Tropical Pacific and with islands in the South Pacific at various distances from the continent were two outstanding features of the marine flora. A partial blockage of species exchange partially accounted for a peculiar latitudinal pattern of species richness that increases with increasing latitudes. In contrast, there was reduced species richness in comparison with other climatically equivalent regions that have contact with more effective routes of migration. Over the last two decades, more than 30 taxonomic and...
Tipo: Journal article Palavras-chave: Benthic phytogeography; Isolation; Latitudinal patterns; Seaweeds; Temperate South America.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2000000400005
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Aislamiento y caracterización de Streptococcus bovis y estudio inicial para el aislamiento de su bacteriófago Colegio de Postgraduados
López Garrido, Serafín Jacobo.
El objetivo del presente estudio fue obtener un cultivo puro de la bacteria ruminal Streptococcus bovis a partir de líquido ruminal de ovinos alimentados con una dieta alta en sorgo (70 % en base seca). Además, se analizó su actividad fermentativa (producción de AGV y lactato) y el proceso de liofilizado como método de conservación, y se realizaron ensayos para expresar su bacteriófago. Para el aislamiento se usó un medio comercial para Streptococcus spp. (Merck) preparado bajo anaerobiosis. Para la identificación se usó el sistema API 20 Strep, reacción catalasa, morfología celular y tinción de Gram. Con la prueba API 20 Strep se comprobó que la bacteria aislada pertenece a la especie S. bovis. Esta bacteria tiene forma esférica a ovoide, de 0.8...
Tipo: Tesis Palavras-chave: Rumen; Streptococcus bovis; Aislamiento; Iiofilización; Bacteriófago; Doctorado; Ganadería; Isolation; Lyophilization; Bacteriophage.
Ano: 2008 URL: http://hdl.handle.net/10521/1402
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An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems J. Venom. Anim. Toxins incl. Trop. Dis.
Gómez,GN; Nerli,BB; Acosta,OC; Picó,GA; Leiva,LCA.
Snake venoms are rich sources of active proteins that have been employed in the diagnosis and treatment of health disorders and antivenom therapy. Developing countries demand fast economical downstream processes for the purification of this biomolecule type without requiring sophisticated equipment. We developed an alternative, simple and easy to scale-up method, able to purify simultaneously protease and phospholipase A2 toxins from Bothrops alternatus venom. It comprises a multiple-step partition procedure with polyethylene-glycol/phosphate aqueous two-phase systems followed by a gel filtration chromatographic step. Two single bands in SDS-polyacrylamide gel electrophoresis and increased proteolytic and phospholipase A2 specific activities evidence the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Partition; Snake toxins; Isolation; Phospholipase A2; Proteases.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300008
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Anthocyanins standards (cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside) isolation from freeze-dried açaí (Euterpe oleraceae Mart.) by HPLC Ciênc. Tecnol. Aliment.
Gouvêa,Ana Cristina Miranda Senna; Araujo,Manuela Cristina Pessanha de; Schulz,Daniel Filisberto; Pacheco,Sidney; Godoy,Ronoel Luis de Oliveira; Cabral,Lourdes Maria Corrêa.
Availability of analytical standards is a critical aspect in developing methods for quantitative analysis of anthocyanins. The anthocyanins cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside were isolated from samples of freeze-dried açaí (Euterpe oleraceae Mart.), which is a round and purple well-known palm fruit in Brazil, and then used as standards. The isolation of the anthocyanins was performed by High Performance Liquid Chromatography (HPLC), using an adapted six-channel selection valve. The identification of anthocyanin pigments in açaí was based on mass spectrometric data for molecular ions and MS-MS product ions and on previous published data. After the collection procedure, standards with a high purity grade were obtained and an external standard...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthocyanins; Standard; Isolation; Açaí; Euterpe oleraceae Mart.; HPLC.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100006
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Antimicrobial resistance and virulence gene profiles in P. multocida strains isolated from cats BJM
Ferreira,Thais Sebastiana Porfida; Felizardo,Maria Roberta; Gobbi,Debora Dirani Sena de; Moreno,Marina; Moreno,Andrea Micke.
Cats are often described as carriers of Pasteurella multocida in their oral microbiota. This agent is thought to cause pneumonia, conjunctivitis, rhinitis, gingivostomatitis, abscess and osteonecrosis in cats. Human infection with P. multocida has been described in several cases affecting cat owners or after cat bites. In Brazil, the cat population is approximately 21 million animals and is increasing, but there are no studies of the presence of P. multocida in the feline population or of human cases of infection associated with cats. In this study, one hundred and ninety-one healthy cats from owners and shelters in São Paulo State, Brazil, were evaluated for the presence of P. multocida in their oral cavities. Twenty animals were positive for P....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cat; Pasteurela multocida; Virulence genes; Resistance; Isolation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100271
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Arcobacter butzleri: first isolation report from chicken carcasses in costa rica BJM
Arias,Maria Laura; Cid,Adriana; Fernandéz,Heriberto.
Arcobacter butzleri isolation from chicken carcasses in Costa Rica is reported for the first time. The isolated strains (P and R) were presumptively identified by their phenotypic characteristics. Definitive identification was made using a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. These first isolations indicate the necessity of further investigation about the prevalence, distribution, ecology and interactions with human beings of this and other Arcobacter species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arcobacter butzleri; Chicken carcasses; Isolation; Epidemiology.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200035
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Banana Supply Chains in Indonesia and Australia: Surviving Isolation from End markets AgEcon
Singgih, Shinta; Woods, Elizabeth.
Two banana supply chains (BSC), one in Indonesia (BSC1) and one in Australia (BSC2), are described based on case study research conducted in 2002. BSC1 sources bananas from small farmers for distribution through traditional markets in Jakarta. BSC2 includes farmers supplying to both major and independent retailers in Sydney through a wholesale agency with some farmer ownership. Farmers in both chains live in relative isolation from the main markets for their bananas. Prices for their bananas depend on the market prices in the city, upon which they have little control. This paper focuses on how farmers removed from key markets, deal with buyers to receive fair prices. In both chains, most transactions between farmers and their buyers were completed through...
Tipo: Conference Paper or Presentation Palavras-chave: Farmers; Banana supply chain; Isolation; Trust.; Crop Production/Industries; Industrial Organization.
Ano: 2003 URL: http://purl.umn.edu/24336
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Brucella spp. isolation from dogs from commercial breeding kennels in São Paulo state, Brazil BJM
Keid,Lara B.; Soares,Rodrigo M.; Morais,Zenaide M.; Richtzenhain,Leonardo J.; Vasconcellos,Sílvio A..
Dogs from 12 commercial breeding kennels were submitted to clinical investigation and laboratorial tests for diagnosis of Brucella spp. infection. The sampling was carried out between April 2000 and February 2002 and the laboratorial tests employed were agar gel immunediffusion test (AGID) and blood culture. From 171 dogs examinated, 39 (22.8%) showed at least one clinical sign compatible with brucellosis, 58 (33.91%) were AGID positive and 24 (14.03%) were positive by blood culture. Gram negative bacterial cells with a biochemical pattern compatible with that of bacteria belonging to genus Brucella were isolated from blood specimens of 24 animals. According to Kappa index and McNemar test, the association between AGID and blood culture (k=0.360 with 95%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dogs; Brucellosis; Brucella canis; Diagnosis; Isolation.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100027
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CELL CULTURE ISOLATION OF HEPATITIS E VIRUS GENOTYPE 3 STRAIN OBTAINED FROM HUMAN FECES Acta biol.Colomb.
QUINTERO-GIL,Carolina; MIRAZO,Santiago; PARRA-SUESCÚN,Jaime; LÓPEZ-HERRERA,Albeiro; MAINARDI,Victoria; ARBIZA,Juan; ORDÚZ,Sergio.
ABSTRACT Hepatitis E virus (HEV) is considered one of the leading causes of acute viral hepatitis worldwide, and about 20 million infections and approximately 57 000 deaths occurred every year. However, little is known about the replicative virus cycle due to the absence of a consensus cell culture model. A549 cell line is considered susceptible to HEV genotype 3, however, both viral strain and cell culture conditions could affect the viral isolation in vitro. The objective of this work was to isolate in vitro an HEV-3 strain obtained from human feces. To this, a genotype 3 HEV strain previously identified by genetic characterization was inoculated in A549 monolayers, and incubated for two hours at 37 °C. Five days post-infection, cells were passaged...
Tipo: Journal article Palavras-chave: A549 cells; Hepevirus; Isolation; RNA virus.
Ano: 2019 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300503
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Characterization and comparison of yeasts from different sources for some probiotic properties and exopolysaccharide production Ciênc. Tecnol. Aliment.
YILDIRAN,Hatice; BAŞYİĞİT KILIÇ,Gülden; KARAHAN ÇAKMAKÇI,Aynur Gül.
Abstract A total of 134 strains of yeasts isolated from fruits and vegetables (certain berry fruits, apples, pomegranates, carrots and grapes), free-range chicken feces and dairy products, and from 10 commercial yeast preparations were identified and subjected to analyses to determine their in vitro probiotic properties. Based on 26S rRNA sequence analysis all ten isolates from commercial products were identified as Saccharomyces cerevisiae, and the natural isolates as Candida corpophila, Candida diddensiae, Clavispora lusitaniae, Hanseniaspora opuntiae, Hanseniaspora uvarum, Kazachstania bovina, Kluyveromyces marxianus, Metschnikowia. pulcherrima, Metschnikowia sp., Meyerozyma carribbica, Pichia kluyveri and Wickerhamomyces anomalus. All isolates were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Probiotic yeasts; Autoaggregation; Production; Gastric survival; Isolation.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000600646
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Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi ArchiMer
Zappa, Sebastien; Rolland, Jean-luc; Flament, Didier; Gueguen, Yannick; Boudrant, Joseph; Dietrich, Jacques.
This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequence showed conservation of the active site and structural elements of the E. coli AP. The recombinant AP was purified and characterized. Monomeric and homodimeric active forms were detected, with a monomer molecular mass of 54 kDa. Apparent optimum pH and temperature were estimated at 11.0 and 70 degreesC, respectively. Thus far, P. abyssi AP has been demonstrated to be the most thermostable AP, with half-lives at 100 and 105 degreesC of 18 and 5 h, respectively....
Tipo: Text Palavras-chave: Escherichia coli; Characterization; Isolation; Gene; Alkaline phosphatase; Pyrococcus abyssi; Archaeon.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-1268.pdf
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Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples BJM
Singh,Harkanwaldeep; Rathore,R.S; Singh,Satparkash; Cheema,Pawanjit Singh.
In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon in DNA extracted from E. coli, Listeria, Salmonella and Staphylococcus confirmed the specificity of the primers. Of randomly collected 143 faecal samples comprising human diarrheic stools (43), cattle diarrheic faeces (48) and poultry faecal swabs (52) only 4, 3 and 8,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter jejuni; Isolation; PCR; Spiking.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100022
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Comparative study of Mycobacterium bovis primary isolation methods BJM
Issa,Marina de Azevedo; Soares Filho,Paulo Martins; Fonseca Júnior,Antônio Augusto; Hodon,Mikael Arrais; Santos,Lílian Cristian dos; Reis,Jenner Karlisson Pimenta dos; Cerqueira Leite,Rômulo.
Abstract For the definitive diagnosis of bovine tuberculosis, isolation of the etiologic agent is required. However, there is no consensus on the best methodology for isolation of Mycobacterium bovis in Brazil. This study evaluated the most used decontaminants and culture media in the country, in order to identify the best combination for the Brazilian samples. Three decontaminants - 2% sodium hydroxide (w/v), 0.75% hexadecylpiridinium chloride (w/v) and 5% sulphuric acid (v/v) and four culture media - 7H11 Middlebrook with additives and OADC supplement “A” (7H11 A), the same media with another supplement trademark (7H11 B), tuberculosis blood agar (B83) and Stonebrink's medium were compared. Regarding the isolation, there were no significant differences...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium bovis; Diagnosis; Middlebrook 7H11; Isolation; Sulphuric acid.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100139
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Comparison of methods for mycobacteria isolation from swine feces BJM
Oliveira,Eugenia Márcia de Deus; Rodriguez,César Alejandro Rosales; Rocha,Vivianne Cambuí Mesquita; Ambrosio,Simone Rodriguez; Ohara,Patrícia Miyuki; Amaku,Marcos; Ferreira,Fernando; Dias,Ricardo Augusto; Leão,Sylvia Cardoso; Ferreira Neto,José Soares.
Swine mycobacteriosis is an important cause of carcass condemnation at abattoirs. One of the best ways to recognize the etiologic agent involved, in live animals, is the fecal isolation, as 94% of the lesions are located in the digestive tract. Therefore, the goal of the present study was to compare the performance of four decontamination methods followed by inoculation in three different culture media, totalizing twelve procedures of mycobacteria search from swine fecal samples experimentally contaminated. The swine feces were artificially contaminated with 0.02 g of Mycobacterium avium, PIG-B strain, and subjected to mycobacteria isolation trial. The protocols used were: 1) modified Petroff or basic method; 2) modified Lowenstein-Jensen or acidic method;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium avium; Isolation; Feces; Swine.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400019
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Comunicação interpessoal em três assentamentos de reforma agrária de Unaí-Minas Gerais. Infoteca-e
ROCHA, F. E. de C.; ZOBY, J. L. F.; XAVIER, J. H. V.; GASTAL, M. L..
ABSTRACT: A research project on farming community development has been carried out by Embrapa Cerrados (Savannah Agricultural Research Center of the Brazilian Agricultural Research Corporation) in three Agrarian Reform Settlement Projects (PA): PA Jibóia, PA Paraíso and PA Santa Clara Furadinho, in Unaí country, Minas Gerais State, Brazil. At its initial phase, there was a diagnose and survey of information about the interpersonal interaction among group members. The objective of this article is to discuss the applicability of Moreno Sociometry in mapping the interpersonal relations between the land reform settlers to subsidize development of farming communities. initially, farmers were interviewed and requested to respond to three questions related to...
Tipo: Documentos (INFOTECA-E) Palavras-chave: Assentamento; Reforma agrária; Comunicação; Pequeno produtor; Relação interpessoal; Liderança; Isolamento; Interação; Afinidade; Sociometria; Análise; Rural settlement; Agrarian reform; Communication; Small farmers; Interpessoal relation; Leadership; Isolation; Sociometry; Affinity; Interaction; Analysis.
Ano: 2003 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/568179
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Cultivating the uncultured: limits, advances and future challenges ArchiMer
Alain, Karine; Querellou, Joel.
Since the invention of the Petri dish, there have been continuous efforts to improve efficiency in microbial cultivation. These efforts were devoted to the attainment for diverse growth conditions, simulation of in situ conditions and achievement of high-throughput rates. As a result, prokaryotes catalysing novel redox reactions as well as representatives of abundant, but not-yet cultured taxa, were isolated. Significant insights into microbial physiology have been made by studying the small number of prokaryotes already cultured. However, despite these numerous breakthroughs, microbial cultivation is still a low-throughput process. The main hindrance to cultivation is likely due to the prevailing lack of knowledge on targeted species. In this review, we...
Tipo: Text Palavras-chave: High throughput cultivation; Cell cell communication; Microbial community; Metabolism; Isolation; Culture.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6820.pdf
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Curtobacterium flaccumfaciens pv. flaccumfaciens detection in bean seeds using a semi-selective culture medium BJM
Maringoni,Antonio Carlos; Camara,Renata de Cassia.
The bacterial wilt caused by Curtobacterium flaccumfaciens pv. flaccumfaciens is currently considered one of the most important bacterial bean disease in Brazil. One of the most effective control methods against this disease is the use of healthy seeds. However, no methods are known that could be routinely used to detect this bacterium in bean seeds under Brazilian condition. The aim of this work was to evaluate qualitative and quantitative detection methods for Curtobacterium flaccumfaciens pv. flaccumfaciens in naturally-infected bean seeds, and the detection of this pathogen in thirty bean seed samples, by sowing onto a semi-selective culture medium the leachate obtained from soaked bean seeds. Both the qualitative and quantitative methods were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial wilt; Isolation; Seed pathology; Phaeolus vulgaris.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400009
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Description of Neblinichthys brevibracchium and N. echinasus from the upper Mazaruni River, Guyana (Siluriformes: Loricariidae), and recognition of N. roraima and N. yaravi as distinct species Neotropical Ichthyology
Taphorn,Donald C.; Armbruster,Jonathan W.; López-Fernández,Hernán; Bernard,Calvin R..
Neblinichthys brevibracchium and N. echinasus are new sympatric species from upland tributaries of the Mazaruni River (Essequibo River basin) of Guyana. These two new species are the first Neblinichthys reported from Guyana. Adult males of both new species have short pectoral-fin spines and several series of hypertrophied odontodes covering the entire dorsal surface and along the entire edge of the snout. They are distinguished from each other by dorsal-fin base length/standard length (18.1-22.8% in N. brevibracchium vs. 24.3-27.0% in N. echinasus), dorsal-anal distance/SL (13.7-18.3% in N. brevibracchium vs. 11.9-12.5% in N. echinasus), by having the snout decreasing in steep arc just anterior to eyes and then flattening in area anterior of nares in N....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essequibo; Guiana Shield; Isolation; Endemism; Punk Catfish.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252010000300006
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Detection of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil BJM
Alves,Clebert José; Figueiredo,Salomão Moreira de; Azevedo,Sérgio Santos de; Clementino,Inácio José; Keid,Lara Borges; Vasconcellos,Sílvio Arruda; Batista,Carolina de Sousa Américo; Rocha,Vivianne Cambuí Mesquita; Higino,Severino Silvano.
To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brucella ovis; Isolation; PCR; Ovine; Paraíba State.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200016
Registros recuperados: 63
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