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Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development. Repositório Alice
IVOMOTO, S. T.; SAKURAY, L. M.; FERREIRA, L. P.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; POT, D.; LEROY, T.; VIEIRA, L. G. E.; DOMINGUES, D. S.; PEREIRA, L. F. P..
Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages...
Tipo: Artigo de periódico Palavras-chave: Cafestol; Kahweol; RT-qPCR; Café; Gene expression; High performance liquid chromatography.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1083355
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Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development. Repositório Alice
IVAMOTO, S. T.; SAKURAY, L. M.; FERREIRA, L. P.; KITZBERGER, C. S. G.; SCHOLZ, MARIA B. S.; POT, D.; LEROY, T.; VIEIRA, L. G. E.; DOMINGUES, D. S.; PEREIRA, L. F. P..
Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages...
Tipo: Artigo de periódico Palavras-chave: Cafestol; Kahweol; RT-qPCR; Expressão genética; Café; Composto químico; Lipidio; Biossíntese; Gene; Coffea Arábica.; Gene expression; High performance liquid chromatography..
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1068512
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Diterpenes profile in high-quality Robusta coffee. Repositório Alice
VIENCZ, T.; ACRE, L. B.; RAMALHO, A. R.; ROCHA, R. B.; ALVES, E. A.; BENASSI, M. T..
Studies of genetical enhancement of Coffea canephora through plant selection and cloning have done relevant contributions in agronomic parameters and in the quality of the beverage. Some Robusta coffees produced in the Amazon Region in Brazil, in addition of high productivity and disease resistance, had shown quality of beverage of specialty coffees. The diterpenes are coffee bioactive compounds associated with several benefits for human health. For Coffea arabica, there are indication that these compounds could also impact in cup quality; however, diterpenes data in C. canephora are still scarce. The aim of this study was to characterize the diterpenes profile of Robusta clones from the Amazon Region.
Tipo: Anais e Proceedings de eventos Palavras-chave: Diterpenos; Kahweol; Cafestol; Ouro Preto do Oeste (RO); Rondônia; Amazônia Ocidental; Western Amazon; Café Robusta; Coffea Canephora; Características Agronômicas; Bebida; Qualidade; Agronomic traits; Coffee (beverage); Grain quality; Diterpenoids.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138308
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Natural intervarietal hybrids of Coffea canephora have a high content of diterpenes. Repositório Alice
FRANCISCO, J. S.; DIAS, R. C. E.; ALVES, E. A.; ROCHA, R. B.; DALAZEN, J. R.; MORI, A. L. B.; BENASSI, M. de T..
The present investigation characterized the diterpene profile of Coffea canephora coffees, which are natural intervarietal hybrids of Conilon and Robusta. The impact of genetic diversity and environment on these compounds was also evaluated. Five genotypes (clones 03, 05, 08, 25, and 66) from six growing sites in the State of Rondônia in the western Amazon (Alto Alegre dos Parecis, São Miguel do Guaporé, Nova Brasilândia do Oeste, Porto Velho, Rolim de Moura, and Alto Paraíso) were analyzed. The contents of kahweol, cafestol, and 16-O-methylcafestol in light-medium roasted coffees were assessed by UPLC. Data were analyzed by ANOVA and Tukey's test (p <- 0.05). The contents of cafestol and 16-O-methylcafestol ranged from 96 to 457 mg 100 g?1 and 75 to...
Tipo: Artigo de periódico Palavras-chave: Café Conilon; Diterpeno; Intervarietal hybrids; Kahweol; Cafestol; 16-OMC; UPLC; Rondônia; Amazônia Ocidental; Western Amazon; Café; Coffea Canephora; Café Robusta; Hibrido; Melhoramento Genético Vegetal; Plant breeding; Diterpenoids.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137709
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