SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Queiroz,R.N.; Ramos,E.R.P.; Alves-Do-Prado,W..

The effects induced by nitric oxide (NO) in different tissues depend on direct and/or indirect interactions with K+ channels. The indirect interaction of NO is produced by activation of guanylyl cyclase which increases the intracellular cGMP. Since NO, cGMP and 4-aminopyridine alone induce tetanic fade and increase amplitude of muscular contractions in isolated rat neuromuscular preparations, the present study was undertaken to determine whether or not the neuromuscular effects of NO and 8-Br-cGMP can be modified by 4-aminopyridine. Using the phrenic nerve and diaphragm muscle isolated from male Wistar rats (200-250 g), we observed that L-arginine (4.7 mM) and 8-Br-cGMP (18 µM), in contrast to D-arginine, induced an increase in the amplitude of muscle...

Antagonism by hemoglobin of effects induced by L-arginine in neuromuscular preparations from rats

Palavras-chave: L-arginine; Nitric oxide; CGMP; Skeletal muscle; Tetanic fade; K+ channel.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700016

Ambiel,C.R.; Alves-Do-Prado,Wilson.

Nitric oxide (NO)-synthase is present in diaphragm, phrenic nerve and vascular smooth muscle. It has been shown that the NO precursor L-arginine (L-Arg) at the presynaptic level increases the amplitude of muscular contraction (AMC) and induces tetanic fade when the muscle is indirectly stimulated at low and high frequencies, respectively. However, the precursor in muscle reduces AMC and maximal tetanic fade when the preparations are stimulated directly. In the present study the importance of NO synthesized in different tissues for the L-Arg-induced neuromuscular effects was investigated. Hemoglobin (50 nM) did not produce any neuromuscular effect, but antagonized the increase in AMC and tetanic fade induced by L-Arg (9.4 mM) in rat phrenic nerve-diaphragm...

Combined aliskiren and L-arginine treatment has antihypertensive effects and prevents vascular endothelial dysfunction in a model of renovascular hypertension

Palavras-chave: Skeletal muscle; Nitric oxide; Tetanic fade; L-arginine; D-arginine; Hemoglobin.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400017

Santuzzi,C.H.; Tiradentes,R.V.; Mengal,V.; Claudio,E.R.G.; Mauad,H.; Gouvea,S.A.; Abreu,G.R..

Angiotensin II is a key player in the pathogenesis of renovascular hypertension, a condition associated with endothelial dysfunction. We investigated aliskiren (ALSK) and L-arginine treatment both alone and in combination on blood pressure (BP), and vascular reactivity in aortic rings. Hypertension was induced in 40 male Wistar rats by clipping the left renal artery. Animals were divided into Sham, 2-kidney, 1-clip (2K1C) hypertension, 2K1C+ALSK (ALSK), 2K1C+L-arginine (L-arg), and 2K1C+ALSK+L-arginine (ALSK+L-arg) treatment groups. For 4 weeks, BP was monitored and endothelium-dependent and independent vasoconstriction and relaxation were assessed in aortic rings. ALSK+L-arg reduced BP and the contractile response to phenylephrine and improved...

Efecto de la suplementación de L-arginina y aceite de pescado en el comportamiento reproductivo de ovejas de pelo

Palavras-chave: 2K1C hypertension; Endothelial dysfunction; Aliskiren; L-arginine; RAAS and oxidative stress.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100065

Colegio de Postgraduados

Bulbarela García, Gerónimo Fermín.

El objetivo del estudio fue evaluar el efecto de la suplementación de L-arginina (ARG) y aceite de pescado (AP) en el tiempo de respuesta (TR), porcentaje de estros (PE), tasa ovulatoria (TO), porcentaje de gestión (PG) y prolificidad (PR). Los trámites fueron: P. Progestágeno (n=7), PMSG. AFG + PMSG (Gonadotropina Sérica de Yegua Preñada) 400 UI (n=15), ARG. AFG +L-argimina 300 mg kg1 (n=17), ARGAP. AFG + L-argimina 300 mg kg1 más aceite de pescado 6 % (n=16) y AP. AFG + aceite de pescado 6% (n=15). Se encontraron diferentes significativas (P≤0.05) para TR en las ovejas del tratamiento PMSG vs P, ARG y ARGAP y AP. El PE fue diferente (P≤0.05) en PMSG y AP vs P, ARG, ARGAP. La TO sólo fue diferente (P≤0.05) en las ovejas adultas de los tratamientos ARG y...

Tipo: Tesis

Palavras-chave: L-arginina; Aceite de pescado; Acetato de fluorogestona; Tasa ovulatoria; Prolificidad; L-arginine; Fish oil; Fluorogestone acetat; Ovulatory rate; Prolificity.

Ano: 2007

URL: http://hdl.handle.net/10521/1198

Effect of L-arginine, dimercaptosuccinic acid (DMSA) and the association of L-arginine and DMSA on tissue lead mobilization and blood pressure level in plumbism

Malvezzi,C.K.; Moreira,E.G.; Vassilieff,I.; Vassilieff,V.S.; Cordellini,S..

Lead (Pb)-induced hypertension is characterized by an increase in reactive oxygen species (ROS) and a decrease in nitric oxide (NO). In the present study we evaluated the effect of L-arginine (NO precursor), dimercaptosuccinic acid (DMSA, a chelating agent and ROS scavenger), and the association of L-arginine/DMSA on tissue Pb mobilization and blood pressure levels in plumbism. Tissue Pb levels and blood pressure evolution were evaluated in rats exposed to: 1) Pb (750 ppm, in drinking water, for 70 days), 2) Pb plus water for 30 more days, 3) Pb plus DMSA (50 mg kg-1 day-1, po), L-arginine (0.6%, in drinking water), and the combination of L-arginine/DMSA for 30 more days, and 4) their respective matching controls. Pb exposure increased Pb levels in the...

Effects of L-arginine on the diaphragm muscle twitches elicited at different frequencies of nerve stimulation

Palavras-chave: L-arginine; DMSA; Tissue lead mobilization; Plumbism; Blood pressure; Rat.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000016

Queiroz,R.N.; Alves-Do-Prado,Wilson.

In rats, the nitric oxide (NO)-synthase pathway is present in skeletal muscle, vascular smooth muscle, and motor nerve terminals. Effects of NO were previously studied in rat neuromuscular preparations receiving low (0.2 Hz) or high (200 Hz) frequencies of stimulation. The latter frequency has always induced tetanic fade. However, in these previous studies we did not determine whether NO facilitates or impairs the neuromuscular transmission in preparations indirectly stimulated at frequencies which facilitate neuromuscular transmission. Thus, the present study was carried out to examine the effects of NO in rat neuromuscular preparations indirectly stimulated at 5 and 50 Hz. The amplitude of muscular contraction observed at the end (B) of a 10-s...

L-arginine, a nitric oxide precursor, reduces dapsone-induced methemoglobinemia in rats

Palavras-chave: Skeletal muscle; Nitric oxide; L-arginine; Neuromuscular transmission; Tetanic fade; S-nitroso-N-acetylpenicillamine; Superoxide dismutase.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600020

BJPS

Moraes,Natália Valadares de; Bergamaschi,Mateus Machado; Bianchi,Maria de Lourdes Pires; Bragheto,Juliana Bordinassi; Malfará,Wilson Roberto; Queiroz,Regina Helena Costa.

Dapsone use is frequently associated to hematological side effects such as methemoglobinemia and hemolytic anemia, which are related to N-hydroxylation mediated by the P450 enzyme system. The aim of the present study was to evaluate the influence of L-arginine supplementation, a precursor for the synthesis of nitric oxide, as single or multiple dose regimens on dapsone-induced methemoglobinemia. Male Wistar rats were treated with L-arginine at 5, 15, 30, 60 and 180 mg/kg doses (p.o., gavage) in single or multiple dose regimens 2 hours prior to dapsone administration (40 mg/kg, i.p.). The effect of the nitric oxide synthase inhibitor L-NAME was investigated by treatment with multiple doses of 30 mg/kg (p.o., gavage) 2 hours before dapsone administration....

Liver lipid peroxidation and antioxidant capacity in cerulein-induced acute pancreatitis

Palavras-chave: Dapsone; Methemoglobinemia; L-arginine; Nitric oxide; L-NAME.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100010

Batcioglu,K.; Gul,M.; Uyumlu,A.B.; Esrefoglu,M..

The aim of this study was to evaluate the role of oxidative damage in pancreatitis-induced hepatic injury. Thirty-five rats were divided into five groups (each of 7 rats): control, cerulein (100 µg/kg body weight), cerulein and pentoxifylline (12 mg/kg body weight), cerulein plus L-NAME (10 mg/kg body weight) and cerulein plus L-arginine (160 mg/kg body weight). The degree of hepatic cell degeneration differed significantly between groups. Mean malondialdehyde levels were 7.00 ± 2.29, 20.89 ± 10.13, 11.52 ± 4.60, 18.69 ± 8.56, and 8.58 ± 3.68 nmol/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively. Mean catalase activity was 3.20 ± 0.83, 1.09 ± 0.35, 2.05 ± 0.91, 1.70 ± 0.60, and 2.85 ± 0.47 U/mg protein for...

Oral administration of L-arginine decreases blood pressure and increases renal excretion of sodium and water in renovascular hypertensive rats

Palavras-chave: Acute pancreatitis; Liver injury; Oxidative damage; Pentoxifylline; L-NAME; L-arginine.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900001

Gouvêa,S.A.; Moysés,M.R.; Bissoli,N.S.; Pires,J.G.P.; Cabral,A.M.; Abreu,G.R..

The two-kidney, one-clip renovascular (2K1C) hypertension model is characterized by a reduction in renal flow on the clipped artery that activates the renin-angiotensin system. Endothelium dysfunction, including diminished nitric oxide production, is also believed to play a role in the pathophysiology of this model. Some studies have shown an effect of L-arginine (L-Arg, a nitric oxide precursor) on hypertension. In the present study we determined the ability of L-Arg (7 days of treatment) to reduce blood pressure and alter renal excretions of water, Na+ and K+ in a model of 2K1C-induced hypertension. Under ether anesthesia, male Wistar rats (150-170 g) had a silver clip (0.20 mm) placed around the left renal artery to produce the 2K1C renovascular...

Parámetros reproductivos en ovejas de pelo suplementadas con glicerol, aceite de pescado y L-arginina.

Palavras-chave: L-arginine; Nitric oxide; Renovascular hypertension; Sodium excretion; Renal physiology; Blood pressure.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700017

Colegio de Postgraduados

Méndez Castillo, Román.

Entre los factores ambientales que influyen en la reproducción de las ovejas, el nivel de nutrición es uno de los más importantes, por ello el estimulo nutricional con alimentación focalizada es de gran utilidad para programar los eventos individuales de los procesos reproductivos. Se realizaron dos experimentos para determinar el efecto de la suplementación de glicerol (G), aceite de pescado (AP), L-arginina (AR) y L-glutamina (GLU) a ovejas Pelibuey en la época reproductiva y de anestro estacional antes de la manifestación del estro, en la incidencia del estro (IE), en la tasa ovulatoria (TO), en tasa de retorno al estro (RE), en tasa de gestación (TG) y en la prolificidad. Al inicio de los experimentos las ovejas fueron sincronizadas al estro con un...

Palavras-chave: Pelibuey; Epoca de anestro; Epoca reproductiva; Glicerol; L-arginina; Aceite de pescado; L-glutamina; Anestrous season; Reproductive season; Glycerol; L-arginine; Oil fish; L-glutamine; Ganadería; Doctorado.

Ano: 2012

URL: http://hdl.handle.net/10521/1713

Role of Natural Antioxidants in the Modulation of Plasma Amino Acid Pattern in Rats Exposed to Hemic Hypoxia

BABT

Al-Rasheed,Nouf Mohamed; Fadda,Laila Mohamed; Al-Rasheed,Nawal Mohamed; Attia,Hala; Ali,Hanaa Mahmoud; El-Agami,Hanaa.

ABSTRACTThe aim of this work was to investigate whether the free radical scavengers, L-arginine (L-arg) and/or carnosine, either alone, or in combination would modulate tissue injury induced by hypoxia by measuring Fischer's ratio [concentrations of branched chain amino acids (BCAAs)/aromatic amino acids]. Decreased Fischer's ratios and increased malondialdehyde (MDA) led to pathogeneses of many diseases. Rats were injected with sodium nitrite (60 mg/kg) to establish hypoxia. They were treated with L-arg, (200 mg/ kg) and/or carnosine (200 mg/ kg) and their combination 24 and 1 h prior to sodium nitrite intoxication. The results revealed that hypoxia significantly decreased hemoglobin, arginine, citrulline and proline and increased sLDH, MDA , ammonia ,...

Palavras-chave: Natural antioxidants; Amino acids; Carnosine; L-arginine; Fisher's ratio; Urea.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500741

The in vivo effect of L-arginine on skin elasticity in mice

BJPS

Souza,Ádria do Prado Barros de; Oliveira,Marina Miranda Rocha de; Andrade,Rafael Rocha de; Amorim,Rivadávio Fernandes Batista de; Bocca,Anamélia Lonrezetti; Borin,Maria de Fátima.

ABSTRACT The human skin aging process is a complex mechanism that can be induced both by intrinsic and extrinsic factors. Observations include a decrease in the biosynthetic and proliferative capacity of cells, increased expression of matrix metalloproteinases, reduction in collagen type I expression, and the progressive disappearance of elastic tissue in the papillary dermis. L-arginine, the substrate of nitric oxide synthesis, is involved in angiogenesis and cell proliferation, as well as an indirect precursor of collagen synthesis via the proline pathway. The aim of this study was to examine the tensile strength, histology, and immunohistochemistry of female and male mice skin receiving different concentrations of topically applied L-arginine, in order...