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Application of a Genus-Specific LAMP Assay for Schistosome Species to Detect Schistosoma haematobium x Schistosoma bovis Hybrids 5
Crego-vicente, Beatriz; Fernández-soto, Pedro; Febrer-sendra, Begoña; García-bernalt Diego, Juan; Boissier, Jérôme; Angora, Etienne K.; Oleaga, Ana; Muro, Antonio.
Schistosomiasis is a disease of great medical and veterinary importance in tropical and subtropical regions caused by different species of parasitic flatworms of the genus Schistosoma. The emergence of natural hybrids of schistosomes indicate the risk of possible infection to humans and their zoonotic potential, specifically for Schistosoma haematobium and S. bovis. Hybrid schistosomes have the potential to replace existing species, generate new resistances, pathologies and extending host ranges. Hybrids may also confuse the serological, molecular and parasitological diagnosis. Currently, LAMP technology based on detection of nucleic acids is used for detection of many agents, including schistosomes. Here, we evaluate our previously developed...
Tipo: Text Palavras-chave: LAMP; Schistosomiasis; Schistosome hybrids; Schistosoma haematobium; Schistosoma bovis; Molecular diagnosis; Species-specific LAMP; Genus-specific LAMP.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00686/79840/82659.pdf
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Development of a Rapid and Sensitive Method for Detection of African Swine Fever Virus Using Loop-Mediated Isothermal Amplification 52
Wu,Xulong; Xiao,Lu; Wang,Yin; Yang,Zexiao; Yao,Xueping; Peng,Bin.
ABSTRACT A loop-mediated isothermal amplification (LAMP) assay was developed for rapid, sensitive and specific detection of African swine fever virus (ASFV). A set of LAMP primers was designed based on the sequence of the ASFV gene K205R. Reaction temperature and time were optimized to 64 oC and 60 min, respectively. LAMP products were detected by agarose gel electrophoresis or visually with the addition of fluorescent dye. The detection limit of the LAMP assay was approximately 6 copies of the target gene per microliter, 100 times more sensitive than conventional PCR. LAMP is a simple and inexpensive molecular assay format for ASFV detection. To date, African swine fever has not been reported in China. LAMP can be used to monitor ASFV spread into China,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: African swine fever virus; LAMP; K205R gene; Molecular biology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200400
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Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes 19
Thekisoe, Oriel M.M.; Rambritch, Natasha E.; Nakao, Ryo; Bazie, Raoul S.; Mbati, Peter; Namangala, Boniface; Malele, Imna; Skilton, Robert A.; Jongejan, Frans; Sugimoto, Chihiro; Kawazu, Shin-Ichiro; Inoue, Noboru.
We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. The primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of T. parva. The detection limit of each primer set is 1 fg, which is equivalent to one copy of the PIM and p150 T. parva genes. These PIM and p150 LAMP primer sets amplify DNA of T. parva isolates from cattle and buffalo from different countries including Kenya, South...
Palavras-chave: Theileria parva; LAMP; Buffalo; Cattle; Diagnosis.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2672
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Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR 52
Ma,Min Min; Jiang,Dan; Li,Shuang Ya; Wu,Yuan; Meng,Ju; Huang,Jiang Yao.
ABSTRACT Adulterant herbal materials are threats to import and export trade and consumer safety. In this study, we established a simple and rapid examination system for the detection of Phellodendron chinense Schneid. Two detection methods, real-time fluorescence quantitative PCR (real-time PCR) and loop-mediated isothermal amplification (LAMP), were developed for traditional Chinese medicine detection, and their specificity and sensitivity were compared. The DNA of P. chinense was extracted and its special periods amplified with designed primers. Real-time PCR and LAMP experiments were conducted to test the specificity of primers in contrast to other similar species. The template concentration was diluted from 101 ng/µL to 10-5 ng/µL in order to contrast...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Traditional Chinese medicine; Real-time PCR; Phellodendron chinense Schneid; LAMP; Specificity; Sensitivity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100314
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Serum markers as an aid in the diagnosis of pulmonary fungal infections in AIDS patients 55
Passos,Ana Isabela Morsch; Dertkigil,Rachel Polo; Ramos,Marcelo de Carvalho; Busso-Lopes,Ariane Fidelis; Tararan,Cibele; Ribeiro,Erivan Olinda; Schreiber,Angélica Zaninelli; Trabasso,Plinio; Resende,Mariangela Ribeiro; Moretti,Maria Luiza.
ABSTRACT Introduction: The etiology of pulmonary infections in HIV patients is determined by several variables including geographic region and availability of antiretroviral therapy. Materials and methods: A cross-sectional prospective study was conducted from 2012 to 2016 to evaluate the occurrence of pulmonary fungal infection in HIV-patients hospitalized due to pulmonary infections. Patients’ serums were tested for (1-3)-β-D-Glugan, galactomannan, and lactate dehydrogenase. The association among the variables was analyzed by univariate and multivariate regression analysis. Results: 60 patients were included in the study. The patients were classified in three groups: Pneumocystis jirovecii pneumonia (19 patients), community-acquired pneumonia (18...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pulmonary infection; HIV/AIDS; (1-3)-β-D-Glugan; LDH; LAMP; Pneumocystosis; Fungal infection.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600606
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Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis 19
Thekisoe, Oriel M. M.; Kuboki, Noritaka; Nambota, Andrew; Fujisaki, Kozo; Sugimoto, Chihiro; Igarashi, Ikuo; Yasuda, Jun; Inoue, Noboru.
In this study, we developed loop-mediated isothermal amplification (LAMP) for the specific detection of both animal and human trypanosomosis using primer sets that are designed from 5.8S rRNA-internal transcribed spacer 2 (ITS2) gene for Trypanosoma brucei gambiense, 18S rRNA for both T. congolense and T. cruzi, and VSG RoTat 1.2 for T. evansi. These LAMP primer sets are highly sensitive and are capable of detecting down to 1 fg trypanosomal DNA, which is equivalent to 0.01 trypanosomes. LAMP is a rapid and simple technique since it can be carried out in 1 h and requires only a simple heating device for incubation. Therefore, LAMP has great potential of being used for diagnosis of trypanosomosis in the laboratory and the field, especially in countries that...
Palavras-chave: LAMP; Trypanosomosis; Trypanosoma brucei brucei; T. b. rhodesiense; T. b. gambiense; T. congolense; T. cruzi; T. evansi.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1045
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Stability of Loop-Mediated Isothermal Amplification (LAMP) Reagents and its Amplification Efficiency on Crude Trypanosome DNA Templates 19
THEKISOE, Oriel M. M; BAZIE, Raoul S. B; CORONEL-SERVIAN, Andrea M; SUGIMOTO, Chihiro; KAWAZU, Shin-ichiro; INOUE, Noboru; 井上, 昇.
This study evaluated the stability of LAMP reagents when stored at 25C and 37C, and also assessed its detection efficiency on different DNA template preparations. Accordingly, LAMP using reagents stored at 25C and 37C amplified DNA of in vitro cultured T. b. brucei (GUTat 3.1) from day 1 to day 15 of reagent storage. There were no significant differences (P>0.05) in detection sensitivity of LAMP among the reagents stored at 25C, 37C and –20C (recommended storage temperature). LAMP using the reagents stored at above-mentioned temperatures amplified serially diluted DNAs (genomic DNA extracted by phenol-chloroform method, FTA card and hemolysed blood) of T. b. gambiense (IL2343) with high sensitivity. Reactions were conducted on the reagents stored...
Palavras-chave: Diagnosis; DNA template; LAMP; Parasitic disease; Trypanosoma.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2691
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