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ACTIVIDAD DE LA LACTICO DEHIDROGENASA (LDH) EN EMERITA ANALOGA (STIMPSON 1857) CRUSTACEA, HIPPIDAE Gayana
Hernández,Klaudia L.; González,Rodrigo R.; Quiñones,Renato A..
Emerita analoga (Stimpson 1857) habita en la zona infralitoral de playas arenosas, desarrollando intensa movilidad en la zona de barrido y embate de las olas. Su habilidad motriz, reflejada en una constante actividad muscular explosiva, sugiere una alta actividad glicolítica (fermentativa) complementaria a la fosforilación oxidativa. En este trabajo se determinó la actividad específica aparente de la láctico dehidrogenasa (LDH EC 1.1.1.27) de E. analoga como indicador de hipoxia funcional. Además, se estudió el efecto del peso y del pH sobre la actividad LDH, así como en los valores aparentes de sus parámetros cinéticos (Vmax y Km). Los resultados indican que los organismos grandes ( a 1,5) y pequeños (< a 1,5 mm) de E. analoga, se ajustan a una curva...
Tipo: Journal article Palavras-chave: Emerita analoga; Metabolismo anaeróbico; Láctico dehidrogenasa; LDH; Actividad de la LDH; Alometría.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382003000100009
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Concentração sérica das enzimas creatinoquinase, aspartato aminotransferase e dehidrogenase lática em equinos da raça crioula Ciência Rural
Da Cás,Elisiane Lourdes; Rosauro,Aura Chaves; Silva,Carlos António Mondino; Brass,Karin Eriça.
A concentração de creatinoquinase (CK), aspartato aminotransferase (AST) e dehidrogenase lática (LDH) foi determinada em amostras de soro obtidas de 60 equinos da raça Crioula: 20 éguas mantidas no pasto (Grupo A), 20 equinos em treinamento (Grupo B) e 20 participantes da competição do "Freio de Ouro de 1997" em Esteio - RS (Grupo C), dos quais foram colhidas amostras 24 a 48 horas antes do início da competição e 24 e 48 horas após a mesma. Não houve variação significativa na LDH. O grupo B apresentou concentrações de CK e AST mais elevadas (p<0,05) que os grupos A e C, indicando uma adaptação ao exercício. Quarenta e oito horas após a competição, a CK alcançou a sua concentração mais baixa e, a AST, a mais elevada. Isso indica que a CK e AST são mais...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CK; AST; LDH; Eqüinos.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000400011
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Cytotoxic and apoptotic effects of scorpion Leiurus quinquestriatus venom on 293T and C2C12 eukaryotic cell lines J. Venom. Anim. Toxins incl. Trop. Dis.
Omran,M. A. A..
Scorpion venom toxicity is of major concern due to its influence on human activities and public health. The cytotoxicity and apoptosis induced by scorpion L. quinquestriatus venom on two established eukaryotic cell lines (293T and C2C12) were analyzed. Both cultured cell lines were incubated with varying doses (10, 20, and 50 µg/ml) of scorpion venom in serum free medium (SFM) for 0.5, 1, 2, 4, and 8 hours at 37°C. The percentage of total lactate dehydrogenase (LDH) released in the culture during venom incubation was used as an index of cell damage. Control culture was treated with an equal amount of SFM. Cell injury was recognized morphologically and apoptosis was researched by a Fluorescing Apoptosis Detection System using the principle of TUNEL...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scorpion venom; Leiurus quinquestriatus; Cytotoxicity; Apoptosis; LDH.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200008
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Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2) Genet. Mol. Biol.
Rizo,Walace Fraga; Ferreira,Luis Eduardo; Colnaghi,Vanessa; Martins,Juliana Simões; Franchi,Leonardo Pereira; Takahashi,Catarina Satie; Beleboni,Rene Oliveira; Marins,Mozart; Pereira,Paulo Sérgio; Fachin,Ana Lúcia.
Cancer has become a major public health problem worldwide and the number of deaths due to this disease is increasing almost exponentially. In the constant search for new treatments, natural products of plant origin have provided a variety of new compounds to be explored as antitumor agents. Tabernaemontana catharinensis is a medicinal plant that produces alkaloids with expressive antitumor activity, such as heyneanine, coronaridine and voacangine. The aim of present study was firstly to screen the cytotoxic activity of the indole alkaloids heyneanine, coronaridine and voacangine against HeLa (human cervix tumor), 3T3 (normal mouse embryo fibroblasts), Hep-2 (human laryngeal epithelial carcinoma) and B-16 (murine skin) cell lines by MTT...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 3T3; Apoptosis; Comet assay; Indole alkaloid; LDH.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100015
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Localization of Total Proteins and Lactate Dehydrogenase in Hamster Epididymis International Journal of Morphology
Beu,Celia C. L.; Orsi,Antonio M; Domeniconi,Raquel F; Novelli,Ethel L. B..
The concentration of total protein measured by photocolorimetric methodology and reported as units per 100 mg of tissue decreased from the initial segment to the cauda epididymidis of the Golden hamster, being significant the numeric difference observed between these two regions. This observation was related with an increased synthesis and secretion of proteins to the lumen in proximal segments of the epididymidis duct, mainly in initial segment, as proposed for other rodents. LDH activity was higher in initial segment and distal cauda than in the caput and corpus epididymidis, although no significant differences in mean values had been observed. The high LDH activity observed in initial segment and cauda epididymidis of hamster had been related to an...
Tipo: Journal article Palavras-chave: Total protein; LDH; Tissue localization; Epididymis; Hamster.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200003
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Nickel incorporation in Fe(II, III) hydroxysulfate Green Rust: effect on crystal lattice spacing and oxidation products Rev. Bras. Ciênc. Solo
Chaves,Lucia Helena Garófalo; Curry,Joan Elizabeth; Stone,David Andrew; Carducci,Michael D.; Chorover,Jon.
Ni(II)-Fe(II)-Fe(III) layered double hydroxides (LDH) or Ni-containing sulfate green rust (GR2) samples were prepared from Ni(II), Fe(II) and Fe(III) sulfate salts and analyzed with X ray diffraction. Nickel is readily incorporated in the GR2 structure and forms a solid solution between GR2 and a Ni(II)-Fe(III) LDH. There is a correlation between the unit cell a-value and the fraction of Ni(II) incorporated into the Ni(II)-GR2 structure. Since there is strong evidence that the divalent/trivalent cation ratio in GR2 is fixed at 2, it is possible in principle to determine the extent of divalent cation substitution for Fe(II) in GR2 from the unit cell a-value. Oxidation forms a mixture of minerals but the LDH structure is retained if at least 20 % of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isomorphous substitution; Layered double hydroxide; LDH; X ray diffraction.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000500005
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Retrospective study of eleven Holstein dairy cows in which bovine leukosis was suspected but ruled out OAK
猪熊, 壽; 堀内, 雅之; 古林, 与志安; INOKUMA, Hisashi; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu.
臨床的に牛白血病を疑ったものの,牛白血病ではないと確定診断されたホルスタイン種乳牛11症例の臨床所見を回顧的に整理した.11症例は体表リンパ節腫大あるいは体腔内の腫瘤触知により牛白血病との鑑別診断が必要であったが,病理解剖により乳房炎,子宮蓄膿症,膿瘍,肺炎,腸炎またはアミロイド症と診断された.牛白血病ウイルス(Bovine leukemia virus: BLV)抗体陽性を呈した4頭のうち,リンパ球増多症を呈したものが2頭であった.またこれら2頭のうち1頭はリンパ節細胞診でも大型リンパ球優勢であった.しかし,血清チミジンキナーゼ(Tymidine kinase: TK),総乳酸脱水素酵素(Lactate dehydrogenase: LDH),LDHアイソザイム2および3の活性値は4頭とも基準値以内であり,牛白血病発症は否定的であった.BLV抗体陰性牛7頭のうち,リンパ節細胞診を実施した2頭は小型リンパ球優勢であり,また,リンパ球増多症を呈した症例はいなかった.1症例では血清TK活性値が基準値をわずかに超えており,また,他の2頭は総LDH,LDHアイソザイム2および3の活性値がいずれも基準値を超えていたが,他の検査項目は牛白血病について否定的であった.腫大リンパ節の細胞診,リンパ球数算定,血清TK,総LDH,LDHアイソザイム2および3の活性値測定といった牛白血病診断のための検査を複数実施することで,牛白血病を否定するための診断精度を高めることが可能と考えられた.
Palavras-chave: 牛白血病; 鑑別診断; 血清チミジンキナーゼ; LDH; LDHアイソザイム.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4367
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Serum markers as an aid in the diagnosis of pulmonary fungal infections in AIDS patients BJID
Passos,Ana Isabela Morsch; Dertkigil,Rachel Polo; Ramos,Marcelo de Carvalho; Busso-Lopes,Ariane Fidelis; Tararan,Cibele; Ribeiro,Erivan Olinda; Schreiber,Angélica Zaninelli; Trabasso,Plinio; Resende,Mariangela Ribeiro; Moretti,Maria Luiza.
ABSTRACT Introduction: The etiology of pulmonary infections in HIV patients is determined by several variables including geographic region and availability of antiretroviral therapy. Materials and methods: A cross-sectional prospective study was conducted from 2012 to 2016 to evaluate the occurrence of pulmonary fungal infection in HIV-patients hospitalized due to pulmonary infections. Patients’ serums were tested for (1-3)-β-D-Glugan, galactomannan, and lactate dehydrogenase. The association among the variables was analyzed by univariate and multivariate regression analysis. Results: 60 patients were included in the study. The patients were classified in three groups: Pneumocystis jirovecii pneumonia (19 patients), community-acquired pneumonia (18...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pulmonary infection; HIV/AIDS; (1-3)-β-D-Glugan; LDH; LAMP; Pneumocystosis; Fungal infection.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600606
Registros recuperados: 8
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