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Screening of Verticillium lecanii (Lecanicillium spp.) Hybrid Strains Based on Evaluation of Pathogenicity against Cotton Aphid and Greenhouse Whitefly, and Viability on the Leaf Surface OAK
相内, 大吾; 馬場, ゆき子; 稲見, 圭吾; 新屋, 良治; 谷, 昌幸; 倉持, 勝久; 堀江, 早弥佳; 小池, 正徳.
In a previous study, protoplast fusion was performed among 3 strains of the entomopathogenic fungi Verticillium lecanii, as a result of which 174 hybrid strains were obtained. The aim of the present study was to screen desirable hybrid strains that have a wide host range or durable effects. Initially, 43 hybrid strains were used in bioassays against the cotton aphid. Of these strains, 30 induced equal or higher mortality as compared to Vertalec (41.9%). Secondly, 50 hybrid strains were used in bioassays against the greenhouse whitefly. Of these strains, 37 exhibited an equal or higher infection rate as compared to that of Mycotal (36.2%). Finally, 50 hybrid strains were applied to cucumber leaves in order to test strain viability under low humidity...
Palavras-chave: Biological control; Lecanicillium spp; Protoplast fusion; Screening; Verticillium lecanii.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2587
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Variation in growth at different temperatures and production and size of conidia in hybrid strains of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) OAK
AIUCHI, Daigo; BABA, Yukiko; INAMI, Keigo; SHINYA, Ryoji; TANI, Masayuki; KOIKE, Masanori; 相内, 大吾; 谷, 昌幸; 小池, 正徳.
In a previous study, we confirmed alterations in several fungal traits in hybrid strains of Verticillium lecanii generated via protoplast fusion. In the present study, we surveyed radial colony growth in different temperature regimes as well as conidia productivity and conidia size of hybrid strains of V. lecanii to elucidate the various primary fungal properties of these strains and to determine the correlation between these properties and virulence to insect pests. Minimum temperature thresholds for all tested strains (except Vertalec®) were below 5°C. Optimal temperatures for colony growth were 20–25°C. Colony growth at high temperature was good or poor depending on the genotype. Temperature and strain significantly affected colony growth. Conidial...
Palavras-chave: Lecanicillium spp; Phenotypic variation; Protoplast fusion; Temperature growth response; Verticillium lecanii.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2848
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