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Registros recuperados: 71
Primeira ... 1234 ... Última
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A brief inventory of sand flies (Diptera, Psychodidae) from the National Forest of the Rio Preto, state of the Espírito Santo, southeastern Brazil Biota Neotropica
Pinto,Israel de Souza; Tonini,João Filipe Riva; Ferreira,Adelson Luiz; Falqueto,Aloísio.
The inventories on sand flies are usually conducted for a long period in leishmaniasis-endemic regions. Thus, there is little data on the sand fly fauna in areas where no leishmaniasis cases have been reported. Therefore, we conducted an inventory to study the sand fly fauna in the National Forest of Rio Preto (NFRP). We carried out a brief inventory by using four CDC light traps in 2009 during winter and summer. We collected 1,403 sand flies belonging to 11 genera and 22 species. As expected, we did not find in the NFRP region sand flies species that have been reported as vectors of Leishmania. However, we found some species of sand flies with few records and that rarely have been reported in southeastern Brazil. Nevertheless, the diversity and species...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Phlebotominae; Diversity; Inventory; Atlantic Forest; Leishmaniasis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000100025
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Actividad leishmanicida y antioxidante de extractos de Piper daniel-gonzalezii Trel. (Piperaceae) Plantas Medicinales
Cardona Galeano,Wilson; Robledo Restrepo,Sara M; Rojano,Benjamín Alberto; Alzate Guarin,Fernando; Muñoz Herrera,Diana Lorena; Saez Vega,Jairo.
Introducción: la leishmaniasis es la enfermedad protozoaria responsable de la mayor morbilidad y mortalidad en la población mundial. Los medicamentos utilizados para su tratamiento presentan serios problemas, entre estos la alta toxicidad y los efectos secundarios severos. Por otro lado, la producción de radicales libres debido al estrés oxidativo ocasiona la oxidación de lípidos, proteínas, DNA y enzimas que son responsables del daño del tejido celular. Algunas especies de la familia Piperaceae presentan un amplio espectro de actividades biológicas, lo cual incita a la exploración de la propiedad antioxidante y antiprotozoaria en miembros del género Piper, como una alternativa en la búsqueda de nuevos medicamentos contra la leishmaniasis y de nuevos...
Tipo: Journal article Palavras-chave: Piper daniel-gonzalezii; Leishmaniasis; Antioxidantes.
Ano: 2013 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962013000200010
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An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran BJID
Farahmand,Mahin; Nahrevanian,Hossein; Shirazi,Hasti Atashi; Naeimi,Sabah; Farzanehnejad,Zahra.
Cutaneous leishmaniasis (CL) is a widespread tropical infection which has a high incidence rate in Iran. Leishmania tropica, the causative agent of anthroponotic cutaneous leishmaniasis (ACL), and Leishmania major, which causes zoonotic cutaneous leishmaniasis (ZCL), are endemic in various parts of Iran with a high incidence rate. The aim of this study was to evaluate the reappraisal of the diagnosis and epidemiology of CL in Iran, by different clinical, parasitological and molecular assays among patients suspected of CL referred to the Department of Parasitology, at the Pasteur Institute of Iran during 2006-2009. Two hundred samples from patients with ulcerative skin lesions were collected, clinical analyses were applied, data questionnaire was completed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Parasitological analysis; Molecular biology; Leishmaniasis; Cutaneous; Iran; Epidemiology.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100004
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Application of the mammalian glyceraldehyde-3-phosphate dehydrogenase gene for sample quality control in multiplex PCR for diagnosis of leishmaniasis J. Venom. Anim. Toxins incl. Trop. Dis.
Gonçalves,SC; Régis-da-Silva,CG; Brito,MEFC; Brandão-Filho,SP; Paiva-Cavalcanti,M.
Leishmaniasis is a neglected disease endemic in five continents. It is a severe disease that may lead to death, and its early detection is important to avoid severe damage to affected individuals. Molecular methods to detect Leishmania are considered alternatives to overcome the limitations presented by conventional methods. The aim of this study was to develop multiplex PCR systems able to detect small amounts of target DNA of Leishmania infantum and Leishmania braziliensis, and the gene coding for glyceraldehyde-3-phosphate dehydrogenase (G3PD) in mammals, enabling quality evaluation of the sample simultaneously with detection of the specific target. The systems created for G3PD recognition were combined with detection systems for L. infantum and L....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Diagnosis; Glyceraldehyde-3-phosphate dehydrogenase; Quality control.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200009
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Cardiac and pulmonary alterations in symptomatic and asymptomatic dogs infectednaturally with Leishmania (Leishmania) chagasi BJMBR
Alves,G.B.B.; Pinho,F.A.; Silva,S.M.M.S.; Cruz,M.S.P.; Costa,F.A.L..
Fifteen symptomatic and seven asymptomatic dogs infected naturally with Leishmania chagasi were examined in order to identify the presence of parasites and changes in heart and lung. Histopathological, cytological, and immunohistochemical analyses were performed on samples of heart and lung tissues. An inflammatory reaction characterized by inflammatory mononuclear, perivascular and intermuscular infiltrates was observed in both symptomatic and asymptomatic animals on histopathological analysis of the heart. In the lung, there was thickening of the alveolar septa due to congestion, edema, inflammatory infiltrate, and fibroblast proliferation. A focal reaction was observed although a diffuse reaction was present in both groups. On cytological examination,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Dog; Lungs; Heart.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300014
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Chalcones and N-acylhydrazones: direct analogues? Exploratory data analysis applied to potential novel antileishmanial agents BJPS
Rando,Daniela Gonçalves; Giarolla,Jeanine; Pasqualoto,Kerly Fernanda Mesquita; Ferreira,Elizabeth Igne.
Leishmaniasis is an important health and social problem for which there is limited effective therapy. Chalcones and N-acylhydrazones have been studied as promising antileishmanial agents in enzymatic inhibition and in vitro assays. Since these chemical classes of compounds also resemble each other structurally, it would be useful to investigate whether they share direct analogy. Exploratory data analysis was applied to a library of chalcones and nitrated N-acylhydrazones assayed against Leishmania donovani to investigate their similarity. Under the conditions applied in the present study, the two classes did not present functional or structural analogy.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chalcones; N-acylhydrazones; Antileishmanial agents; Leishmaniasis; Chemometrics.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200019
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Comparison of different primes for PCR-based diagnosis of cutaneous leishmaniasis BJID
Oliveira,Diego Molina de; Lonardoni,Maria Valdrinez Campana; Teodoro,Ueslei; Silveira,Thais Gomes Verzignassi.
OBJECTIVE: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous leishmaniasis (ACL) diagnosis. MATERIAL AND METHODS: Five pairs of primers, four of them derived from Leishmania kDNA sequences (MP3H-MP1L; B1-B2; LBF1-LBR1; 13A-13B), and one derived from the SL RNA (mini-exon) gene repeat (LU5A-LB3C), reported previously, were used. RESULTS: The MP3H-MP1L primers were the best at amplifying the DNA, detecting 2 fg of Leishmania spp. DNA. The 13A-13B primers presented the worst performance, detecting 512 x 10³ fg of DNA. CONCLUSION: The wide variation in the analytical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Polymerase chain reaction; Leishmania; DNA primers.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300004
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Comparison of the specificity of PCR and the histopathological detection of leishmania for the diagnosis of American cutaneous leishmaniasis BJMBR
Medeiros,A.C.R.; Rodrigues,S.S.; Roselino,A.M.F..
More precise and rapid diagnostic methods for American cutaneous leishmaniasis (ACL) are necessary because of the growing number of cases observed in Brazil, including the northeastern region of the State of São Paulo. We applied PCR to 54 skin or mucosal biopsies from patients with a clinical and/or laboratory diagnosis of ACL using primers 13A and 13B, with positive results being obtained for 82% of the samples. When the PCR results were compared to those of histopathological leishmania detection, PCR showed superior results with 81.5% sensitivity and 95% CI of 68.0-95.1%. The Montenegro skin test (MST) was positive in 88.7% of patients. Since MST cannot be used as a diagnostic tool in endemic areas, the present results strongly suggest the use of PCR...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Cutaneous leishmaniasis; PCR; Histopathological detection; Leishmania detection.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400002
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Contribution to the Knowledge of the Phlebotomine Sand Flies Fauna (Diptera: Psychodidae) of Timóteo Municipality, Minas Gerais, Brazil Neotropical Entomology
Souza,Cristian F; Borges,Magno A Z; Andrade,Andrey J.
An investigation of the phlebotomine sandfloy fauna in the municipality of Timóteo, Minas Gerais State, Brazil, was undertaken with New Jersey traps placed in seven neighborhoods from December 2005 to January 2006. A total of 2,289 phlebotomine sandfloy specimens were recorded. Nyssomyia whitmani (Antunes & Coutinho) (48.1%), Nyssomyia intermedia (Lutz & Neiva) (36.8%) and Micropygomyia quinquefer (Dyar) (7.1%) were the most abundant species sampled. Some sandfloy species that play a role in the transmission of Leishmania Ross in the State of Minas Gerais were recorded and their importance to public health is highlighted. Pintomyia bianchigalatiae (Andrade Filho, Aguiar, Dias & Falcão), Micropygomyia capixaba (Dias, Falcão, Silva...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sandfloy; Leishmaniasis; Phlebotominae; Pressatia choti; Public health.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2009000200016
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Cytokine profile and pathology in human leishmaniasis BJMBR
Ribeiro-de-Jesus,A.; Almeida,R.P.; Lessa,H.; Bacellar,O.; Carvalho,E.M..
The clinical spectrum of leishmaniasis and control of the infection are influenced by the parasite-host relationship. The role of cellular immune responses of the Th1 type in the protection against disease in experimental and human leishmaniasis is well established. In humans, production of IFN-<FONT FACE="Symbol">g</font> is associated with the control of infection in children infected by Leishmania chagasi. In visceral leishmaniasis, an impairment in IFN-<FONT FACE="Symbol">g</font> production and high IL-4 and IL-10 levels (Th2 cytokines) are observed in antigen-stimulated peripheral blood mononuclear cells (PBMC). Moreover, IL-12 restores IFN-<FONT FACE="Symbol">g</font> production and enhances the cytotoxic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Human leishmaniasis; Cytokines; Pathology; Immunological responses.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100020
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Description of the male of Evandromyia gaucha Andrade Filho, Souza, Falcão (Psychodidae, Phlebotominae) Rev. Bras. entomol.
Souza,Getúlio D.; Shimabukuro,Paloma H.F.; Andrade Filho,José D..
Here, we present the description of male of the sand fly species Evandromyia gaucha. Six male specimens of E. gaucha, together with conspecific females, were collected in native forest areas of the municipalities of Porto Alegre, Caçapava do Sul (type locality of E. gaucha) and Santa Cruz do Sul, in Rio Grande do Sul state, Brazil. The specimens are distinguished from other species of the rupicola series of the genus Evandromyia by the presence of dilatation in the apical quarter of the genital filaments followed by tapering of the final portion.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Phlebotominae; Rupicola series.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262015000200085
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DETECCIÓN DE ANTICUERPOS ANTI-Leishmania (Trypanosomatidae) EN POBLACIONES CANINAS DEL DEPARTAMENTO DE SUCRE, COLOMBIA Acta biol.Colomb.
PATERNINA TUIRÁN,Luís Enrique; DÍAZ-OLMOS,Yirys Arleth; PATERNINA-GÓMEZ,Margaret; CARRILLO-BONILLA,Lina María; VÉLEZ,Iván Darío; BEJARANO,Eduar Elías.
El objetivo de la investigación fue determinar la prevalencia de anticuerpos anti-Leishmania en perros del departamento de Sucre, Colombia. Se analizaron 122 perros del área rural de los municipios de Ovejas, Sampués y Sincelejo, mediante la técnica de inmunofluorescencia indirecta (IFI). Anticuerpos anti-Leishmania fueron detectados en el 69,6 % (IC95 % = 61,1 - 78,2) de la población canina estudiada. En el municipio de Sincelejo la seropositividad fue del 72 % (IC95 % = 61,1 - 82,8), en el municipio de Sampués del 68,4 % (IC95 % = 52,3 - 84,5) y en el municipio de Ovejas del 55,5 % (IC95 % = 21,2 - 86,3). Los animales seropositivos fueron principalmente caninos jóvenes y adultos. El 17,6 % de los perros seropositivos mostró signos clínicos compatibles...
Tipo: Journal article Palavras-chave: Colombia; Epidemiología; Leishmaniasis; Seroprevalencia.
Ano: 2016 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000100018
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Distribution of liposome-encapsulated antimony in dogs BJMBR
Schettini,D.A.; Costa Val,A.P.; Souza,L.F.; Demicheli,C.; Rocha,O.G.F.; Melo,M.N.; Michalick,M.S.M.; Frézard,F..
The achievement of complete cure in dogs with visceral leishmaniasis is currently a great challenge, since dogs are the main reservoir for the transmission of visceral leishmaniasis to humans and they respond poorly to conventional treatment with pentavalent antimonials. In order to improve the efficacy of treatment, we developed a novel formulation for meglumine antimoniate based on the encapsulation of this drug in freeze-dried liposomes (LMA). The aim of the present study was to evaluate the biodistribution of antimony (Sb) in dogs following a single intravenous bolus injection of LMA. Four healthy male mongrel dogs received LMA at 3.8 mg Sb/kg body weight and were sacrificed 3, 48 and 96 h and 7 days later. Antimony was determined in the blood, liver,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Liposomes; Meglumine antimoniate; Biodistribution; Leishmaniasis; Dogs.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200015
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Distribution of the assemblage of phlebotomine sandflies (Diptera: Psychodidae) along an environmental gradient Biota Neotropica
Antonini,Yasmine; Fernandes,G. Wilson; Barata,Ricardo Andrade.
Abstract The geographical distribution of leishmaniasis has undoubtedly expanded, and is now being reported to be in areas that were previously non-endemic. This study therefore aimed to investigate whether there is any variation in the structure and in the composition of phlebotomine communities along an environmental gradient - from a mesic to a xeric environment, across different climatic periods (rainy and dry seasons). Furthermore, we test whether there were differences in sex ratio of sand flies between wet and dry environments. A total of 535 specimens of sand flies belonging to 18 species were recorded. Richness, abundance, and composition of species were significatively higher in the xeric environment (semidecidual Forest) compared to the mesic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Species composition; Ecology; Leishmaniasis; Atlantic Forest; Brazil.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032017000200202
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Diversity of sand flies in domiciliary environment of Santarém, state of Pará, Brazil: species composition and abundance patterns in rural and urban areas Acta Amazonica
Feitosa,Marlisson Augusto Costa; Julião,Genimar Rebouças; Costa,Manoel Djalma Pereira; Belém,Braulio; Pessoa,Felipe Arley Costa.
This paper aimed to evaluate the richness, abundance and frequency of sand fly occurrence in rural and urban areas American visceral Leishmaniasis -AVL is endemic in the study area of Santarém municipality, Pará state. Sand flies were collected during 1995-2000, using CDC light traps placed in neighborhoods and rural areas of the municipality. A total of 53.454 individuals and 26 species of sand flies were collected. The most abundant species in both urban and rural environments was Lutzomyia longipalpis, vector of AVL in the area. The highest species richness by capture was in rural area. In all years sampled, the largest number of species of sand fly collected was always in rural areas. The species of sand flies in urban and rural area were similar in 11...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Anthropic impact; Amazon.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672012000400008
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Dosis diagnóstica y umbral de resistencia de Lutzomyia evansi (Diptera: Psychodidae), a dos insecticidas utilizados en salud pública en Colombia: deltametrina y lambdacihalotrina Rev. Soc. Entomol. Argent.
Henriquez,Caterine; Pereira,Yaneth; Cochero,Suljey; Bejarano,Eduar Elías.
Los insecticidas son una herramienta importante para el control de los insectos transmisores de microorganismos patógenos. El objetivo de este estudio fue determinar la dosis diagnóstica de deltametrina y lambdacihalotrina en el flebotomíneo Lutzomyia evansi (Núñez-Tovar), vector de Leishmania infantum en Colombia. Los insectos se recolectaron en la Estación Experimental de Fauna Silvestre de Colosó, Sucre, un área de reserva natural que no ha sido sometida a presión con insecticidas. Los bioensayos se realizaron en botellas de vidrio, siguiendo el método simplificado de determinación de resistencia del CDC. En los experimentos, se usaron hembras silvestres de L. evansi que fueron expuestas a diferentes concentraciones de los insecticidas por espacio de 80...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecticidas; Control vectorial; Leishmaniasis; Colombia.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802009000200006
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Environmental sanitation and peri-domiciliar organisation as auxiliary practices for the control of phlebotomines in Paraná state, southern Brazil BABT
Teodoro,Ueslei; Balduíno,João; Thomaz-Soccol,Vanete; Barbosa,Orlando C; Ferreira,Maria E. M. C; Lozovei,Ana Leuch; Verzignassi,Thaís G; Roberto,Andrea C. B. Silva.
Studies were conducted to compare the results of the collections of phlebotomines, in two distinct periods, i.e. before and after changed environmental conditions in Jussara county, Paraná State, Brazil. Collections were made near to a forest in Jussara farm, between February and April 1992. Six Falcão light traps were installed in houses and animals' shelters. Catches were made once a month and 35,783 phlebotomines were captured. Based on these data, sanitation was done in peridomiciliary area and domestic animals shelters were separated from domiciles in January 1994. Then during February to April 1994 using the same methodology new phlebotomines catches were made. The results showed that an average of just 150 (6,311/42) phlebotomines per hour were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Psychodidae; Phlebotomine; Sandflies; Ecology; Lutzomyia.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300007
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Estudio del perfil electroforético en sueros de caninos con leishmaniasis visceral de Posadas, provincia de Misiones, Argentina InVet
Ramayo,L.G.; Estevez,J.O.; Nevot,M.C.; Stempler,A; Goldman,L.; Jar,A.M.; Mundo,S.L..
La leishmaniasis visceral es una enfermedad zoonótica producida por el protozoario Leishmania infantum (syn. chagasi) transmitida por el flebótomo Lutzomyia longipalpis y que tiene al canino como principal reservorio en áreas urbanas y periurbanas. En el presente trabajo se realizó un estudio electroforético retrospectivo de 40 sueros de caninos enfermos con diagnóstico parasitológico confirmado de leishmaniasis obtenidos entre los años 2006 y 2008 en la ciudad de Posadas, provincia de Misiones, Argentina. El 80 % (32 de 40) de las muestras presentaron alteraciones en el perfil electroforético caracterizadas por la disminución de la relación albúmina/globulina y la presencia de hipergammaglobulinemia de tipo policlonal en el 52,5 % de los casos (21 de 40)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canino; Electroforesis; Leishmaniasis; Hipergammaglobulinemia.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982011000200008
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Estudos Sobre Leishmaniose: I. Primeiros Casos de Leishmaniose Espontânea Observados em Cobáios BABT
Medina,Heitor.
The author describes the first cases of spontaneous leishmaniasis in guinea-pigs, as verified for the first time, in Curitiba, at the Institute of Biology and Technological Research. Studying the inoculability of Paracoccidioide brasiliensis in guinea-pigs, he found animals which presented tumoral lesions in the ears and peritesticular skin. The examination of these tumors, mixed with a small drop of Giemsa's original solution, plus the exam of the rubbings of the same material, coloured by the May-Grunwald Giemsa after fixation by Heidenhein's solution and the histological exams of the cuts in the tumoral lesions revealed the presence of elements which possessed the same morphology and aspect of the LEISHMAN-DONOVANI corpuscles. In the meanwhile, a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Guinea-pig.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500003
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Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction J. Venom. Anim. Toxins incl. Trop. Dis.
Braga,Audrey Rennó Campos; Langoni,Hélio; Lucheis,Simone Baldini.
Background: This study aimed to evaluate the occurrence of Leishmaniaspp. in dogs and cats from Botucatu, São Paulo state, and Campo Grande, Mato Grosso do Sul state, Brazil, by the association of three diagnostic tests: blood culture in liver infusion tryptose medium, immunofluorescent antibody test and polymerase chain reaction. Fifty blood samples of dogs and cats from the Center for Zoonosis Control in Campo Grande, an area endemic for canine visceral leishmaniasis, were collected randomly, as well as canine and feline blood samples from the Municipal Kennel and Animal Protection Association in Botucatu, currently considered a transmission-free, non-endemic area.Results: Of the 50 dog blood cultures from Botucatu, three (6%) were positive and of the 50...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Cat; Diagnosis; Leishmaniasis; Zoonosis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200323
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