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A C-type lectin from Bothrops jararacussu venom can adhere to extracellular matrix proteins and induce the rolling of leukocytes J. Venom. Anim. Toxins incl. Trop. Dis.
Elífio-Esposito,S. L.; Hess,P. L.; Moreno,A. N.; Lopes-Ferreira,M.; Ricart,C. A. O.; Souza,M. V.; Hasselman-Zielinski,F.; Becker,J. A.; Pereira,L. F..
Purification of a lectin from Bothrops jararacussu venom (BjcuL) was carried out using agarose-D-galactose affinity gel. MALDI-TOF gave a major signal at m/z 32028, suggesting the presence of a dimmer composed of two identical subunits. Divalent cations were required for the lectin activity, as complete absence of such ions reduced hemagglutination. BjcuL was more effective at neutral pH and showed total loss of activity at pH values below 4.0 and above 9.0. Its agglutinating activity remained stable at 25°C until 60min, but increased when at 35°C for at least 15min. Adhesion assays to extracellular matrix (ECM) glycoproteins showed that the biotinylated lectin (0.039-5.0µg/100µl) was capable of binding to fibronectin and vitronectin in a dose-dependent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Venoms; Fibronectins; Vitronectin; Snakes; Intravital microscopy; Leukocytes.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400009
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Acute intoxication by deltamethrin in jundia: emphasis on clinical, biochemical and haematological effects. Repositório Alice
GALEB, L. do A. G.; GANECO, L. N.; FREDIANELLI, A. C.; WAGNER, R.; FAM, A. L. P. D'A; ROCHA, D. C. C.; KIRSCHNIK, P. G.; PIMPÃO, C. T..
Deltamethrin (DM) is a synthetic pyrethroid pesticide highly toxic to aquatic organisms. Because of its lipophilic feature it can be highly absorbed by the fish gills, which partially explains the high sensitivity of these animals to DM exposure in concentrations up to a thousand times lower than in mammals. This study compared clinical, biochemical and haematological observations between DM intoxicated and nonintoxicated fish. The experiment involved five exposure conditions (control and four concentrations of DM) and was executed in triplicates with 4 animals per set (n=60). The first behavior changes represented by rapid operculum movement and irregular or on the surface swimming were observed shortly after exposure to the concentrations of 0.5, 1.0 and...
Tipo: Artigo de periódico Palavras-chave: Peixe; Toxidez; Rhamdia quelen; Enzima; Glóbulo branco; Piretrina; Fish; Leukocytes; Pyrethrins; Toxicity.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/960389
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Arachidonic acid triggers an oxidative burst in leukocytes BJMBR
Pompeia,C.; Cury-Boaventura,M.F.; Curi,R..
The change in cellular reducing potential, most likely reflecting an oxidative burst, was investigated in arachidonic acid- (AA) stimulated leukocytes. The cells studied included the human leukemia cell lines HL-60 (undifferentiated and differentiated into macrophage-like and polymorphonuclear-like cells), Jurkat and Raji, and thymocytes and macrophages from rat primary cultures. The oxidative burst was assessed by nitroblue tetrazolium reduction. AA increased the oxidative burst until an optimum AA concentration was reached and the burst decreased thereafter. In the leukemia cell lines, optimum concentration ranged from 200 to 400 µM (up to 16-fold), whereas in rat cells it varied from 10 to 20 µM. Initial rates of superoxide generation were high,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachidonic acid; NADPH oxidase; Nitroblue tetrazolium; Leukocytes; Reactive oxygen species.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100013
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Características hematológicas do tambaqui Colossoma macropomum Cuvier (Osteichthyes, Characidae) em sistema de monocultivo intensivo: II. Leucócitos Zoologia
Tavares-Dias,Marcos; Sandrim,Elziane F. Silva; Campos-Filho,Eugênio de.
The leukocytes parameters in approximately one-year-old freshwater fish Colossoma macropomum Cuvier, 1818 (tambaqui) kept in an intensive monobreeding system as well as the correlation among these parameters and the biometric data (total weight and standard length) were investigated. The mean value of the white blood cell count (WBC) in the peripheral blood of tambaqui was 2663.3±1288µl and in the differential count, the following means were observed: neutrophils (1566.2±754µl); lymphocytes (973.6±447µl); monocytes (86.7±123µl) and special granulocitic cells (7.8±144µl). The blood parameters studied were positively correlated among one another, but were negatively correlated with the standard length. However, no correlation was with the weigth of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Characidae; Colossoma macropomum; Freshwater fish; Leukocytes; Tambaqui.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000100012
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Comparative study on hematological parameters of farmed matrinxã, Brycon amazonicus Spix and Agassiz, 1829 (Characidae: Bryconinae) with others Bryconinae species Acta Amazonica
Tavares-Dias,Marcos; Affonso,Elizabeth Gusmão; Oliveira,Sarah Ragonha; Marcon,Jaydione Luiz; Egami,Mizue Imoto.
The aim of this study was not only to determine the red blood cells parameters, thrombocyte and leukocyte counts in farmed Brycon amazonicus (matrinxã), to compare these parameters among Bryconinae species from literature, and also to investigate the presence of special granulocytic cells in these fish. The results of the blood cells parameters here established for farmed B. amazonicus, a species of great economic importance in Brazilian aquaculture, could help a better understanding of the blood features in natural populations of this Amazon species. Blood parameters varied between Bryconinae species investigated, mainly the red blood cell counts, hemoglobin, hematocrit and mean corpuscular volume (MCV). The presence of the blood granulocytes, neutrophils...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leukocytes; Freshwater fish; Blood; Amazon; Brycon amazonicus; Bryconinae.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000400026
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Composto homeopático reduz a liberação de ânion superóxido pelas células mononucleares de ema (Rhea americana) Arq. Bras. Med. Vet. Zootec.
Bertoldo,W.R; França,J.L; Fernandes,L.T.O; França-Botelho,A.C.
The action of the "Stress Factor Ostrich (Arenales - Fauna and Flora)" was tested in the release of superoxide anion by cells in the peripheral blood of rhea (Rhea americana). Sixteen samples of 0.5mL of venous blood were collected through the jugular vein in the morning and placed in heparinized tubes. The leukocytes were separated into polymorphonuclear (PMN) and mononuclear (MN). The production of superoxide anion by phagocytes of peripheral blood was determined using the chromogen ferricytochrome C. There was a reduction of superoxide by MN cells in the presence of "Stress Factor Ostrich" indicating a positive influence of product against oxidative stress. Furthermore, future researches, such as the evaluation of other reactive oxygen intermediates and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ema; Estresse; Superóxido; Leucócitos; Rhea americana; Stress; Superoxide; Leukocytes.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500027
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Contribution of CD4+ T cells to the early mechanisms of ischemia- reperfusion injury in a mouse model of acute renal failure BJMBR
Pinheiro,H.S.; Camara,N.O.S.; Noronha,I.L.; Maugeri,I.L.; Franco,M.F.; Medina,J.O.A.P.; Pacheco-Silva,A..
Renal ischemia-reperfusion (IR) injury is the major cause of acute renal failure in native and transplanted kidneys. Mononuclear leukocytes have been reported in renal tissue as part of the innate and adaptive responses triggered by IR. We investigated the participation of CD4+ T lymphocytes in the pathogenesis of renal IR injury. Male mice (C57BL/6, 8 to 12 weeks old) were submitted to 45 min of ischemia by renal pedicle clamping followed by reperfusion. We evaluated the role of CD4+ T cells using a monoclonal depleting antibody against CD4 (GK1.5, 50 µ, ip), and class II-major histocompatibility complex molecule knockout mice. Both CD4-depleted groups showed a marked improvement in renal function compared to the ischemic group, despite the fact that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ischemia-reperfusion; Kidney inflammation; Leukocytes; CD4+ T lymphocytes; Kidney transplantation; Acute renal failure.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400015
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Disintegrins: integrin selective ligands which activate integrin-coupled signaling and modulate leukocyte functions BJMBR
Barja-Fidalgo,C.; Coelho,A.L.J.; Saldanha-Gama,R.; Helal-Neto,E.; Mariano-Oliveira,A.; Freitas,M.S. de.
Extracellular matrix proteins and cell adhesion receptors (integrins) play essential roles in the regulation of cell adhesion and migration. Interactions of integrins with the extracellular matrix proteins lead to phosphorylation of several intracellular proteins such as focal adhesion kinase, activating different signaling pathways responsible for the regulation of a variety of cell functions, including cytoskeleton mobilization. Once leukocytes are guided to sites of infection, inflammation, or antigen presentation, integrins can participate in the initiation, maintenance, or termination of the immune and inflammatory responses. The modulation of neutrophil activation through integrin-mediated pathways is important in the homeostatic control of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Integrin; Disintegrin; Leukocytes; Activation; Proliferation; Signal transduction.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000008
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Ecotoxicity of natural insecticide based on tobacco plant extract and hematological effects on the Nile tilapia (Oreochromis niloticus). Biological Sciences
Millan, Debora Cristina; Centro Universitário Central Paulista; Shiogiri, Natália Sayuri; Universidade Federal de São Carlos; Souza, Naiara Elisabete da Silva de; Universidade Federal de São Carlos; Silva, Hugo Ribeiro da; Universidade Federal de São Carlos; Fernandes, Marisa Narciso; Universidade Federal de São Carlos.
Natural insecticides derived from plant extracts have been used as an alternative to synthetic products in order to reduce environmental contamination. The present study aimed to examine the effects of Fumydro®, a natural insecticide based in the tobacco plant Nicotiana tabacum, on the Nile tilapia (Oreochromis niloticus) by determining the 48-h LC50 and evaluating their effects on hematological variables. Adult specimens of O. niloticus were exposed to four Fumydro® concentrations (200, 300, 400 and 500 μL L-1). The 48-h LC50 of Fumydro® was determined as 370 ± 50 μL L-1. Surviving fish showed increasing in the red blood cells, hemoglobin concentration, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration. The thrombocytes did not...
Palavras-chave: Ecologia toxicity; Erythrocytes; Leukocytes; Teleost; Thrombocytes 2.05.03.00-8.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14131
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Effect of fatty acids on leukocyte function BJMBR
Pompéia,C.; Lopes,L.R.; Miyasaka,C.K.; Procópio,J.; Sannomiya,P.; Curi,R..
Fatty acids have various effects on immune and inflammatory responses, acting as intracellular and intercellular mediators. Polyunsaturated fatty acids (PUFAs) of the omega-3 family have overall suppressive effects, inhibiting lymphocyte proliferation, antibody and cytokine production, adhesion molecule expression, natural killer cell activity and triggering cell death. The omega-6 PUFAs have both inhibitory and stimulatory effects. The most studied of these is arachidonic acid that can be oxidized to eicosanoids, such as prostaglandins, leukotrienes and thromboxanes, all of which are potent mediators of inflammation. Nevertheless, it has been found that many of the effects of PUFA on immune and inflammatory responses are not dependent on eicosanoid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fatty acids; Immune system; Inflammation; Dietary lipids; Leukocytes.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100001
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Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss) Gayana
Gómez,Leonardo A; Cortés,Raúl; Smith,Carlos T.
This work studied the effect of the lectin phytohaemagglutinin (PHA) and bacterial lipopolysaccharide (LPS) on the activity of nonspecific cytotoxic cells (NCCs) and macrophages of peripheral blood in the rainbow trout. NCCs are cells of the innate immunity, homologues to Natural Killer (NK) cells of mammals, capable of eliminating cells infected with intracellular pathogens and also tumoral cells. Their lytic activity is mediated by the perforin/granzyme system and the Fas receptor-Fas ligand (FasR-FasL) system. Activity of NCCs was evaluated by their capacity to lyse HL-60 tumor cells. On the other hand, macrophages eliminate microorganisms by several mechanisms, including the production of reactive oxygen species (ROS) and nitric oxide (NO). Results...
Tipo: Journal article Palavras-chave: Interleukins; Innate immunity; FasR-FasL; Lectins; Leukocytes.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382013000100007
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Evaluation of genotoxic and cytotoxic effects of hydroalcoholic extract of Euphorbia tirucalli (Euphorbiaceae) in cell cultures of human leukocytes Anais da ABC (AABC)
Machado,Michel M.; Oliveira,Luis F.S. de; Zuravski,Luisa; Souza,Raul O. de; Fischer,Paula; Duarte,Jonathaline A.; Rocha,Manoelly O.; Güez,Camila M.; Boligon,Aline A.; Athayde,Margareth L..
ABSTRACT Euphorbia tirucalli (L.), commonly known as aveloz, has been indiscriminately used in popular medicine to treat various illnesses. However, some components can have devastating consequences. Injury to a cell's genetic material can cause mutations, cancer, and cell death. Our main goal in this work was to evaluate the genotoxic and cytotoxic effects of E. tirucalli extract on human leukocytes. For this purpose, we performed a phytochemical analysis to evaluate the plant's components. In the second step, we treated cultured human leukocytes with different concentrations of the dry extract of the plant and then evaluated the oxidative and genotoxic profiles of these leukocytes. We found that at 1% and 10% concentrations, the aveloz extract acted as a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aveloz; Euphorbia tirucalli; Leukocytes; Genotoxic; Cytotoxic.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100017
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Expression of the chemokine receptor CXCR4 on lymphocytes of leprosy patients BJMBR
Mendonça,V.A.; Alvim de Melo,G.E.B.; Araújo,M.G.; Borges,V.O.; Costa,R.D.; Martins-Filho,O.A.; Teixeira- Carvalho,A.; Sathler-Avelar,R.; Teixeira,M.M.; Teixeira,A.L..
Leprosy is caused by Mycobacterium leprae, which induces chronic granulomatous infection of the skin and peripheral nerves. The disease ranges from the tuberculoid to the lepromatous forms, depending on the cellular immune response of the host. Chemokines are thought to be involved in the immunopathogenesis of leprosy, but few studies have investigated the expression of chemokine receptors on leukocytes of leprosy patients. In the present study, we evaluated 21 leprosy patients (M/F: 16/5) with a new diagnosis from the Dermatology Outpatient Clinic of the University Hospital, Federal University of Minas Gerais. The control group was composed of 20 healthy members (M/F: 15/5) of the community recruited by means of announcements. The expression of CCR2,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Leprosy; Chemokine receptors; CXCR4; Leukocytes.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200009
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FISH IMMUNE SYSTEM Gayana
Olabuenaga,Susana E..
The defence against infections is the essential function of the immune system of all Vertebrates. Fish present an efficient and developed immunologic response and in the case of Teleosts, with some similarities to the higher vertebrates. It can be divided into two types: innate or non specific and adquired or specific, both with a humoral and cellular compartments. Their relative importance can change according to the age of fish and is always under the influence of different factors. The most relevant components are mentioned here. There are two factors in fishes which strongly influence the immune response and antibody production, seasonal changes and temperature. The main lymphoid organs are described here: the thymus, the kidney and the spleen, as well...
Tipo: Journal article Palavras-chave: Antigen; Antibody; Leukocytes; Phagocytosis; Lymphokines; Inflammation; Immunostimulants.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200010
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Haematological assessment of four amazonian ornamental armoured catfish (Teleostei, Loricariidae) Biological Sciences
Neves, Mikaelle de Souza; Barbas, Luis André Luz; Couto, Márcia Valeria Silva do; Lopes, Josiane Nazaré Silva; Tavares-Dias, Marcos; Fujimoto, Rodrigo Yudi.
The aim of this study was to characterize the haematological profile of four Amazonian ornamental freshwater armoured catfish: acari-bola (Peckoltia oligospila - L06), acari-pleco (Cochliodon sp. - L145), acari-canoa (Lasiancistrus saetiger - L323) and acari-assacu (Pseudocanthicus spinosus - L160). The highest blood glucose levels (72.47 ± 28.7 mg dL-1) and erythrocyte counts (0.51 ± 0.2 x 106 cel. μL-1) were recorded for acari-canoa. The acari-bola and acari-pleco presented similar concentrations of total plasma protein (TPP) (7.96 ± 1.8 and 7.93 ± 1.8 g dL-1, respectively) against lower TPP concentrations observed in acari-canoa (4.87 ± 1.5 g dL-1) and acari-assacu (6.55 ± 1.5 g dL-1). The acari-assacu had lower total haemoglobin concentration (5.88 ±...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquicultura; Hematológia de peixes blood profile; Leukocytes; Fish; Pleco. aquicultura.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/39554
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Hank’s balanced salt solution: an alternative resuspension medium to label autologous leukocytes. Experience in inflammatory bowel disease BABT
Martin-Comin,Joseph; Cardoso,Valbert Nascimento; Plaza,Pedro; Roca,Manoel.
In this work Hank's balanced salt solution (HBSS) has been used, as resuspension medium, instead of leukocyte poor plasma (LPP) to label autologous white blood cells in 28 patients with suspicion af active inflammatory bowel disease.Labelled cells were reinjected and anterior and caudo-craneal views were obtained at 30 min, 2 h and 6 h p.i. Regions of interest were outlined on liver, spleen, lung, bone marrow (spine), background and lesions and the organ/background activity ratios were calculated in all scans. Patients were classified into 2 groups: Group 1: LPP, 30 patients and Groups 2: HBSS, 28 patients. Labelling efficiency was higher in HBBS group (89.0 ± 3.2 %) than in the LPP group (6.5 ± 6.3%). Organ/background activity ratios were similar in both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 99mTechnetium; Leukocytes; Hank' s solution; Inflammatory.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500006
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Hematological and biochemical responses of pirarucu (Arapaima gigas, Arapaimidae) fed with diets containing a glucomannan product derived from yeast and algae Acta Amazonica
HOSHINO,Maria Danielle Figueiredo Guimarães; MARINHO,Renata das Graças Barbosa; PEREIRA,Diogle Farias; YOSHIOKA,Eliane Tie Oba; TAVARES-DIAS,Marcos; OZORIO,Rodrigo Otavio de Almeida; RODRIGUEZ,Anselmo Fortunato Ruiz; RIBEIRO,Ricardo Amaral; FARIA,Fernando Sérgio Escócio Drummond Viana de.
ABSTRACT The hematological and biochemical responses of pirarucu fingerlings (Arapaima gigas) fed with diets containing different concentrations of a glucomannan product derived from yeast and algae were evaluated in order to ascertain the effect of these diets on fish physiology. Four treatments were conducted, with three replications, with 12 fish in each tank. The product evaluated (MycosorbA+(r)) was incorporated into the commercial diet, at four concentrations: 0, 1, 2 and 4 g.kg-1, called M0%, M0.1%, M0.2% and M0.4%, respectively. After 45 days of feeding, blood samples from six fish in each replicate were collected to perform the analyses. Their weight and length were determined to calculate the condition factor and weight gain, but no differences...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorella vulgaris; Saccharomyces cerevisiae; Leukocytes; Albumin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000200087
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Hematological and biometric traits of tuvira Gymnotus inaequilabiatus (Valenciennes, 1839) (Gymnotiformes: Gymnotidae) from the Brazilian Pantanal Anais da ABC (AABC)
RODRIGUES,ROBSON A.; SILVA,EDUARDA S.; MARCONDES,SANDRIELY F.; GALINDO,GIZELA M.; OLIVEIRA,GUSTAVO G. DE; SOUZA,ALDA I. DE; RAGUSA-NETTO,JOSÉ; FERNANDES,CARLOS E..
ABSTRACT This study describes the hematological and biometric characteristics of male and female Gymnotus species from the Pantanal, Mato Grosso do Sul state, Brazil. Fifty adult specimens of Gymnotus inaequilabiatus were weighed, measured, and then euthanized. Blood was collected by puncturing the celiac mesenteric vein to determine the hematocrit, hemoglobin content, number of erythrocytes, mean corpuscular volume, mean corpuscular hemoglobin concentration, glucose level, absolute value of leukocytes, and relative value of leukocytes and thrombocytes. Body weight and relative condition factor did not differ (P > 0.05) between the sexes, as well as erythrogram and the blood glucose values. Hematocrit ranged from 18.0% to 54.0%; hemoglobin from 1.1 to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood glucose; Erythrogram; Leukocytes; Sex differences; Thrombocytes.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100049
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Hematological profile of beef cattle with divergent residual feed intake, following feed deprivation PAB
Gomes,Rodrigo da Costa; Siqueira,Renata Farinelli de; Ballou,Michael Alan; Stella,Tiago Roberto; Leme,Paulo Roberto.
The objective of this work was to characterize the hematological profiles of steers and bulls, according to residual feed intake (RFI), after feed deprivation. Twenty‑month‑old Nellore steers and bulls were fed feedlot diets for 70 and 56 days, respectively. RFI was calculated as the difference between actual feed intake and expected feed intake. More and less efficient steers and bulls, according to RFI, were subjected to 24 hours of food deprivation. Blood was sampled prior to and following the withdrawal period. Hematological analyses included total and differential white blood cell count, red blood cell count and morphology, and plasma glucose concentration. Variation in RFI did not influence plasma glucose concentration or blood cell profiles. Glucose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bos indicus; Fasting; Feed efficiency; Leukocytes; Stress.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000900019
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Hepatocytes, rather than leukocytes reverse DNA damage in vivo induced by whole body y-irradiation of mice, as shown by the alkaline comet assay Biol. Res.
PINCHEIRA,JUANA; CARRERA,PILAR; MARCELAIN,KATHERINE; DE LA TORRE,CONSUELO.
DNA damage repair was assessed in quiescent (G0) leukocytes and in hepatocytes of mice, after 1 and 2 hours recovery from a single whole body y-irradiation with 0.5, 1 or 2 Gy. Evaluation of single-strand breaks (SSB) and alkali-labile sites together were carried out by a single-cell electrophoresis at pH>13.0 (alkaline comet assay). In non-irradiated (control) mice, the constitutive, endogenous DNA damage (basal) was around 1.5 times higher in leukocytes than in hepatocytes. Irradiation immediately increased SSB frequency in both cell types, in a dose-dependent manner. Two sequential phases took place during the in vivo repair of the radio-induced DNA lesions. The earliest one, present in both hepatocytes and leukocytes, further increased the SSB...
Tipo: Journal article Palavras-chave: Alkaline comet assay; Ionizing radiation; Hepatocytes; Leukocytes; In vivo DNA repair.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200011
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