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LI,Li; ZHU,Wenyi; YANG,Jingxiu; LIU,Xiaolan; DONG,Yinmao. |
Abstract Objective This study was to establish a rapid quantitative method for determination of six characteristic flavonoids, including licochalcone A (1), liquiritigenin (2), glabridin (3), isoliquiritigenin (4), liquiritoside (5), and isoliquiritoside (6) in licorice by a ultra-performance convergence chromatography (UPC2) apparatus equipped with a photodiode-array detector (PDA). Methods: The flavonoids studied were separated on an ACQUITY UPC2 TM Torus 2-PIC column (2.1 × 100 mm, 1.7 μm) using gradient elution (85:15–70:30) with a mobile phase consisting of CO2 and methanol: formic acid (99.8:0.2, v/v), back pressure of 2,000 psi, flow rate of 0.8 mL/min, and detection at 254 nm. This method was further applied for the analysis of the flavonoids... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Licorice; UPC2/PDA; Quantify; Flavonoids. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000600426 |
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