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A comparison of paraffin and resin-based techniques used in bark anatomy Naturalis
Hamann, T.; Smets, E.; Lens, F..
Bark anatomy is an unappreciated discipline in plant systematics, despite its great potential to reveal systematically informative features. In this paper, main reasons for the lack of detailed bark anatomical data in many plant families are identified, including problems with sectioning, terminological issues, and difficulties in observation of dilated stems. We deal with these problems by focusing on two aspects: (1) compare, discuss and improve existing sectioning and maceration techniques using two species with soft and hard bark tissues; and (2) discuss the best way to collect stem bark samples. We hope that this paper will stimulate inclusion of bark anatomical data in future systematic studies.
Tipo: Article / Letter to the editor Palavras-chave: Bark anatomy; Light microscopy; Macerations; Plant systematics; Phloem; Sectioning; 42.54.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/421834
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A histological study of microsporogenesis in Tarenna gracilipes (Rubiaceae) Naturalis
Vinckier, S.; Smets, E..
We studied the microsporogenesis in Tarenna gracilipes (Hayata) Ohwi, with special attention to the mode of exine deposition and tapetum development. We based this research on light (LM), scanning electron (SEM) and transmission electron microscopic (TEM) observations of developing anthers of T. gracilipes, from the microspore mother cell stage towards anther dehiscence. Evidence is supplied that the microsporogenesis in T. gracilipes can be considered as simultaneous. Columellae, foot layer and tectum develop in a fibrillar matrix. Similar with earlier studies in Rubiaceae species, a single white line formed near the plasmalemma in the extra-apertural region. The developing endexine dilated into several white line centered lamellae at the apertures. An...
Tipo: Article / Letter to the editor Palavras-chave: Tarenna gracilipes; Rubiaceae; Microsporogenesis; Exine deposition; Tapetum development; Histology; Light microscopy; Scanning.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407305
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Anatomical and biochemical changes associated with the development and germination of Araucaria angustifolia seeds Biological Sciences
Shibata, Marília; Coelho, Cileide Maria Medeiros; Schmidt, Eder Carlos; Bouzon, Zenilda Laurita; Campos, José Marcello Salabert de; Maraschin, Marcelo.
This study aimed at assessing the cell cycle, and anatomical and biochemical changes that the embryonic axis of Araucaria angustifolia undergoes during development, focusing on the maturation stage. During all development, cells exhibited intense metabolic activity with an abundance of mitochondria, lipid bodies, and vacuolated cells. The continued accumulation of starch and protein was observed by LM and TEM and indicated by spectra of FTIR. Cell differentiation of the procambium was observed with a thickening of the cell wall and the formation of resiniferous ducts. At Stage III and IV, cells exhibited structural changes such as altered or elongated mitochondria and presence of plastoglobules. These results suggest that there is a gradual transition from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.03.00.00-0 Botânica; 2.03.03.00-9 Fisiologia Vegetal; 5.02.01.04-2 Sementes Florestais maturation; Recalcitrant seed; Light microscopy; Transmission electron microscopy; Flow cytometry Botânica.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/43381
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Anatomical and histological study of the liver and pancreas of two closely related mountain newts Neurergus microspilotus and N. kaiseri (Amphibia: Caudata: Salamandridae) Rev. Bras. Zool.
Vaissi,Somaye; Parto,Paria; Sharifi,Mozafar.
ABSTRACT Anatomical and histological examinations were conducted on the digestive glands of two closely related mountain newts, Neurergus microspilotus (Nesterov, 1916) and Neurergus kaiseri Schmidt, 1952. In N. microspilotus and N. kaiseri the major digestive glands comprise a very large liver and a small pancreas. In both species the liver has two distinct lobes, right and left. Histologically, the parenchyma of the liver of both species is contained within a thin capsule of fibroconnective tissue. Glycogen deposits and fat storage often dissolve during the routine histological process and produce considerable histological variability. Sinusoids are lined with endothelial cells forming a very thin epithelial sheet, with discontinuous basement membrane....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestive glands; Light microscopy; Hematoxylin-Eosin; Periodic acid-Schiff (PAS).
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702017000100306
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Bovine placentome preservation for light microscopy evaluation Arq. Bras. Med. Vet. Zootec.
Martins,E.; Martins,V.M.V.; Marques Júnior,A.P.; Vasconcelos,A.C.; Chiarini-Garcia,H.; Malard,P.F..
This work evaluates the preservation of bovine placentomes using paraformaldehyde and glutaraldehyde at 4% and solutions of Karnovsky, Bouin and Carnoy for 4, 12 and 24 hours fixation, at room temperature or at 4ºC, before inclusion in plastic resin and paraffin. The best preservation of the specimens was obtained after 12 hours of fixation with those solutions containing aldehydes. With Bouin solution the best time of fixation was 4 hours, while for Carnoy solution the best time of fixation was 24 hours. Inclusion in plastic resin gave better results than in paraffin, and the temperature did not influence the quality of preservation of the specimens of bovine placentomes for evaluation with light microscopy.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Placentome; Light microscopy.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000200006
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Comparative Wood Anatomy of the Primuloid Clade (Ericales s.l.) Naturalis
Lens, F.; Jansen, S.; Caris, P.; Serlet, L.; Smets, E..
The wood structure of 78 species from 27 genera representing the woody primuloids (Maesaceae, Myrsinaceae, and Theophrastaceae) was investigated using light microscopy (LM) and scanning electron microscopy (SEM). Results indicated that the ray structure, the nature of mineral inclusions, and the occurrence of breakdown areas in rays can be used to separate the three primuloid families from each other. Within Ericales, the presence of exclusively multiseriate rays is synapomorphic for Myrsinaceae and Theophrastaceae, and the occurrence of breakdown areas in rays is synapomorphic for Myrsinaceae. Within Myrsinaceae, the wood structure of the mangrove genus Aegiceras differs because it has short vessel elements that are storied, non-septate fibers, a...
Tipo: Article / Letter to the editor Palavras-chave: Primuloids; Ericales s.l.; Maesaceae; Myrsinaceae; Theophrastaceae; Wood anatomy; Comparison; Light microscopy; Scanning electron microscopy.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407307
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Diversity of foliar endophytic fungi from the medicinal plant Sapindus saponaria L . and their localization by scanning electron microscopy Biol. Res.
García,Adriana; Rhoden,Sandro A; Rubin Filho,Celso J; Nakamura,Celso V; Pamphile,João A.
Endophytic fungi inhabit vegetable tissues or organs, without causing them any harm. Endophytes can co-evolve with plant hosts and possess species-specific interactions. They can protect the plant from insect attacks and diseases, and are also able to produce substances of biotechnological interest. In folk medicine, the bark, roots and fruits of Sapindus saponaria is used to produce substances with anxiolytic, astringent, diuretic and expectorant properties, as well as tonics, blood depuratives and cough medicine. This study evaluated the diversity of endophytic fungi present in the leaves of S. saponaria L. and observed the colonization of host plants by endophytes, using light and scanning electron microscopy. We verified that these fungi are found in...
Tipo: Journal article Palavras-chave: Endophyte; Light microscopy; Phylogenetic analysis; SEM.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200006
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Endophytic interaction of Bacillus sp. in micropropagated banana plantlets Anais da ABC (AABC)
ROCHA,JOSIELE S.; NIETSCHE,SILVIA; PEREIRA,MARLON C.T.; MARIA O.,MERCADANTE-SIMÕES; SANTOS,RAYANE C.; XAVIER,ADELICA A..
Abstract: The banana tree is associated with different species of endophytic bacteria that can stimulate plant growth. However, further studies are needed to better understand the relationships between this group of bacteria and the host plant. The objective of this study was to investigate the localization of the EB-40 (Bacillus sp.) through anatomical and ultrastructural analyses in micropropagated banana plantlets. The results demonstrated the effective colonization of the EB-40 isolate in the intercellular and intracellular spaces, as well as in the rhizosphere region. The wall of endophytic bacteria contains calcium and nitrogen. The EB-40 isolate was also observed to associate with the plasma membrane and cell wall. These results further our...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Musa spp.; Light microscopy; Transmission electron microscopy..
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500855
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Histomorphological changes by epididymal lithiasis in roosters Rev. Bras. Ciênc. Avic.
Geraldo,I; Mahecha,GAB; Martins,NRS.
Epididymal lithiasis (EL) histopathology is described using light and electronic microscopy in roosters (Gallus gallus domesticus) naturally affected by EL in Minas Gerais, Brazil. The histologic and morphological changes by EL in roosters was performed regarding cellular and subcellular details through light and electron microscopy. Efferent ductules epithelium lysosomal increase in size and numbers, membrane rupture, cellular vacuolation, ciliary loss, basal membrane degeneration, inflammatory reaction with mononuclear infiltrations, edema, epithelial and vascular endothelium losses were described. All industrial and freerange chickens showed EL in varying degrees in the efferent ductules (ED). However, ED altered areas did not correlate with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epididymal lithiasis; Histopathology; Light microscopy; Electron microscopy; Gallus gallus domesticus; Infertility; Rooster; Testicular calculus.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000100004
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Light Microscopic and Ultrastructural Studies on Sarcocystis spp. Infection in Philippine Water Buffaloes (Bubalus bubalis) OAK
Claveria, Florencia G.; Cruz, Mary Jane.
Palavras-chave: Light microscopy; Ultrastructure; Sarcocystis spp.; Philippine water buffaloes.
Ano: 1999 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/312
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Light Microscopic Studies of Sarcocystis spp. Infection in Cattle Slaughtered in Three Different Abattoirs in Metro Manila OAK
Claveria, Florencia G.; Farolan, Renee Joy; Macabagdal, Miriam Rose; Criss, Alvin; Salvador, Rene; Lim, Roima San-Pedro.
Palavras-chave: Bovine sarcocystosis; Sarcocystis; Light microscopy; Philippines.
Ano: 1999 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/313
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Methods for glomerular quantification in dogs: a comparative study Ciência Rural
Costa,Caio Augusto Leles; Lima,Charles Silva de; Uscategui,Ricardo Andres Ramirez; Silva,Gyl Eanes Barros; Crivellenti,Leandro Zuccolotto.
ABSTRACT: It was evaluated the different methodologies for glomerular enumeration described in the literature in a quantitative, double-blind study on the number of glomeruli present in kidney biopsy specimens obtained using a Tru-cut-type biopsy needle. Eighteen samples were taken (n=18) from various regions of canine kidneys harvested immediately after euthanasia. Sample collection was carried out using Tru-cut-type, semi-automated, 16 gauge needles. Reading and evaluation of the specimens were performed independently by four researchers by means of eye loupe inspection, light microscopy, light microscopy with a reduced condenser aperture, and dissection microscopy. Samples were also subjected to histopathological evaluation for comparison purposes....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glomerulopathy; Histopathology; Light microscopy.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000300600
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Microscopic aspects of electrosensory system on the partially euryhaline lesser guitarfish Anais da ABC (AABC)
RANGEL,BIANCA S.; WOSNICK,NATASCHA; AMORIM,ALBERTO F.; K. JUNIOR,JOSÉ ROBERTO; RICI,ROSE E.G..
ABSTRACT The electrosensory system on elasmobranchs consists of subcutaneous electroreceptor organs known as ampullae of Lorenzini. The present study investigated the ampullae of Lorenzini morphology of the lesser guitarfish Zapteryx brevirostris, using light microscopy and scanning electron microscopy. The pore number found in the ventral skin surface is much higher than that found in the dorsal portion, characteristic of species that inhabit the euphotic zone. Under light microscopy it was possible to observe that the wall canal consists of a single layer of squamous epithelial cells. The canal features distal expansion, where the ampullae are located with up to six alveoli. The sensory epithelium of ampullae is composed by cubic cells, with oval...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Elasmobranch; Ampullae of Lorenzini; Electroreceptors; Light microscopy; Scanning electron microscopy.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100037
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Microscopic Studies on Erythrocytes of Channa punctata Exposed to Commercial Grade Lindane BABT
Bhattacharjee,Debasish; Das,Suchismita.
ABSTRACT Light and scanning electron microscopic studies of erythrocytes of Channa punctata exposed to two sublethal doses of lindane over a period of 21 days revealed myriads of anomalies. High frequency of micronucleus appeared progressively in the erythrocyte, ranging from 3.8-7.5%, when monitored weekly for 21 d at 0.25 g/L and 1.0-3.0% at 0.025 g/L lindane. Prominent structural anomalies of erythrocytes included abnormal shapes, vacuolation, membrane invagination and disintegration. At higher dose and at later duration, a unique tendency of abnormal cells to stick together was observed. It could be concluded that commercial formulation of lindane could induce genotoxicity and structural anomalies in the erythrocytes of fish.
Tipo: Info:eu-repo/semantics/article Palavras-chave: RBC; SEM; Light microscopy; Micronucleus.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100449
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MORPHOLOGICAL ALTERATIONS IN THE MASSETER MUSCLE INDUCED BY UNILATERAL EXTRACTION OF THE UPPER MOLARS IN GUINEA PIGS (Cavia porcellus) International Journal of Morphology
Mardegan Issa,João Paulo; Mizusaki Iyomasa,Mamie; Ioshie Mizusaki,Cristina; Watanabe,Ii-sei; Mandarim de Lacerda,Carlos Alberto.
The objective of the present study was to analyze morphological aspects of the structures of the stomatognathic system and their relationship with malocclusion. For this purpose, we determined the morphological alterations that occur in the masseter muscle of guinea pigs (Cavia porcellus) after the induction of malocclusion by extraction of the left upper molars. Animals were divided into two groups (malocclusion and control group) and submitted to macroscopic, biometric and light microscopic analyses. The results showed a reduction in the width of the masseter muscle mainly in left side in the malocclusion group 60 days after unilateral extraction. Microscopically, round and polygonal muscle fibers of several diameters were observed, and altered muscle...
Tipo: Journal article Palavras-chave: Masseter muscle; Morphology; Light microscopy; Muscle fibers; Guinea pig.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000300009
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Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae Neotropical Entomology
Pinheiro,Daniela O.; Quagio-Grassiotto,Irani; Gregório,Elisa A..
The sugarcane borer, Diatraea saccharalis Fabricius, is a pest to sugarcane and many other crops. This work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of D. saccharalis larvae. Fragments of the midgut (anterior, middle and posterior regions) were fixed and processed by light and scanning electron microscopy. There are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depending on their localization along the midgut. The apical surface of columnar cells shows an increase in both number and size of the apical protrusions from the anterior to the posterior midgut regions. There is an increase in the amount of PAS-positive (Periodic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphology; Sugarcane borer; Histochemistry; Scanning electron microscopy; Light microscopy.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000400009
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Organogenesis from internodal segments of yellow passion fruit Scientia Agricola
Biasi,Luiz Antonio; Falco,Maria Cristina; Rodriguez,Adriana P. M.; Mendes,Beatriz M. Januzzi.
Organogenesis induction from internodal segments of yellow passionfruit (Passiflora edulis f. flavicarpa) was obtained in a medium supplemented with 1.0-4.0 mg L-1 benzylaminopurine (BAP). Callus proliferation and adventitious shoot development were analyzed by light and scanning electron microscopy. Callus proliferation and adventitious shoots were observed simultaneously and continuously, with asynchronous development of shoots in the explants. Proliferation of callus was observed in the explant cut ends in all treatments, including the control. The results show that concentrations of 1.0 or 2.0 mg L-1 BAP gave better rate of organogenesis. Higher concentrations were efficient for the induction of adventitious buds and, were, however, detrimental for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passiflora edulis f. flavicarpa; Adventitious shoots; Light microscopy; Scanning electron microscopy; Benzylaminopurine.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400010
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Pollen morphology of Centaurea L. (Asteraceae) in Iran Acta Botanica
Shabestari,Elaheh Sadat Bagheri; Attar,Farideh; Riahi,Hosein; Sheidai,Masoud.
The genus Centaurea L. is one of the largest genera in the family Asteraceae. The number of taxa included in this genus ranges from 500 to 600 worldwide. Iran is one of the major centers of diversity for this genus. Pollen morphology has proved useful in the systematics of Asteraceae, as well as in that of some of its genera and species. The pollen morphology of 19 taxa of the genus Centaurea was investigated in detail by light and scanning electron microscopy. Examination under light microscopy showed pollen grains to be tricolporate, isopolar, radially symmetrical, oblate spheroidal, prolate spheroidal, subprolate, tectum perforate and scabrate. Based on the exine ornamentation observed under scanning electron microscopy, we identified two types of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Centaurea; Palynology; Exine ornamentation; Light microscopy; Scanning electron microscopy.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400004
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Post-seminal development and morphoanatomy of vegetative and reproductive organs in Stevia rebaudiana (Bert.) Bertoni (Asteraceae) Anais da ABC (AABC)
ROSSI,MÔNICA L.; SOUZA,EVERTON H.; GRANER,ERIKA M.; ALMEIDA,MARCÍLIO DE; MARTINELLI,ADRIANA P..
Abstract Stevia rebaudiana belongs to the Asteraceae family with high economic and medicinal potential. This article describes and illustrates morphological and histological aspects of leaves and reproductive organs, and the germination process, to provide detailed information on this species and to contribute to taxonomic, phylogenetic and pharmacobotanical projects. The fruit is a cypsela, small, simple, dry, indehiscent, monospermic, light or dark colored, with aristate pappus, and the seed presents a spatulate axile embryo. Germination is phaneroepigeal with a pivotal root system and many absorbing root hairs. The leaves are simple, elliptical to obovate, with two types of trichomes (glandular and tector), with a short petiole, exhibiting an opposite...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Germination; Light microscopy; Morphology; Scanning electron microscopy.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502167
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Pseudocyphellae ontogeny and thallus anatomy in species of Punctelia Krog (Parmeliaceae, lichenized Ascomycota) Acta Botanica
Zanetti,Camila Aparecida; Barbosa,Suzana Bissacot; Adachi,Sérgio Akira; Marcelli,Marcelo Pinto.
ABSTRACT The genus Punctelia has been traditionally characterized by the type and distribution of pseudocyphellae, medullary chemical substances and the shape of conidia, whereas its species have been distinguished using mainly the shape, location, abundance and size of pseudocyphellae. Given that Punctelia is not monophyletic, additional studies are needed to determine its current delimitation. The present study used conventional techniques for structural studies using optical and scanning electron microscopy in order to describe and compare the structure and ontogeny of the pseudocyphellae of five species of Punctelia. The main differences among the studied species involve the type of pseudocyphellae development, the presence or absence of an epicortex...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeroplectenchyma; Lichen anatomy; Light microscopy; Parmeliaceae; Plectenchyma; Pseudocyphellae; Punctelia.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100051
Registros recuperados: 24
Primeira ... 12 ... Última
 

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