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Registros recuperados: 11
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A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4 BABT
Acevedo,Francisca; Pizzul,Leticia; Castillo,María del Pilar; Rubilar,Olga; Lienqueo,María Elena; Tortella,Gonzalo; Diez,María Cristina.
In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead . The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50ºC) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.
Tipo: Info:eu-repo/semantics/article Palavras-chave: White-rot fungi; Ligninolytic enzymes; Purification; Lignocellulosic material.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600013
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Biodegradation of polycyclic aromatic hydrocarbons by soil fungi BJM
Clemente,Andrea R.; Anazawa,Tania A.; Durrant,Lucia R..
Thirteen deuteromycete ligninolytic fungal strains were grown in media containing polycyclic aromatic hydrocarbons (PAHs), for 6 and 10 days. The PAHs were added directly with the inocula or on the third day of cultivation. A selection of the best strains was carried out based on the levels of degradation of the PAHs and also on the ligninolytic activities produced by the fungi. The selected strains were cultivated for 3, 6, 9, 12 and 15 days in the PAHs-containing media. Degradation of PAHs, as measured by reversed-phase HPLC on a C18 column, varied with each strain as did the ligninolytic enzymes present in the culture supernatants. Highest degradation of naphthalene (69%) was produced by the strain 984, having Mn-peroxidase activity, followed by strain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Ligninolytic enzymes; Polycyclic aromatic hydrocarbons; Fungi.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400001
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Characterization of fungal inoculum used in soil bioremediation BJM
Ballaminut,Nara; Matheus,Dácio R..
Studies have indicated the capacity of basidiomycetes to degrade recalcitrant organopollutants. However, the age of the fungal inoculum to obtain a more effective degradation has not been defined. The criterion used is total colonization of the substrate. Psilocybe castanella CCB444 and Lentinus crinitus CCB274 have been evaluated in soils containing hexachlorobenzene. In the present study, the physiological conditions of the fungal inocula were characterized on solid substrate (sugarcane bagasse, starch and soy flour). Colonization of the substrate, loss of organic matter, pH variation, organic carbon, total nitrogen, fungal biomass and enzymatic activity were evaluated over 30 days of incubation. Colonization of the substrate was almost complete after 20...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ligninolytic enzymes; Basidiomycetes; Fungal inoculum; Ergosterol.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200011
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Decolorization of remazol brilliant blue R with laccase from Lentinus crinitus grown in agro-industrial by-products Anais da ABC (AABC)
ALMEIDA,PATRÍCIA H.; OLIVEIRA,ANA CAROLINA C. DE; SOUZA,GENYFER P.N. DE; FRIEDRICH,JULIANA C.; LINDE,GIANI A.; COLAUTO,NELSON B.; VALLE,JULIANA S. DO.
ABSTRACT Lentinus crinitus is a white-rot fungus that produces laccase, an enzyme used for dye decolorization. Enzyme production depends on cultivation conditions, mainly agro-industrial by-products. We aimed to produce laccase from Lentinus crinitus with agro-industrial by-products for dye decolorization. Culture medium had coffee husk (CH) or citric pulp pellet (CP) and different nitrogen sources (urea, yeast extract, ammonium sulfate and sodium nitrate) at concentrations of 0, 0.7, 1.4, 2.8, 5.6 and 11.2 g/L. Enzymatic extract was used in the decolorization of remazol brilliant blue R. CH medium promoted greater laccase production than CP in all evaluated conditions. Urea provided the greatest laccase production for CH (37280 U/L) as well as for CP...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dye decolorization; Ligninolytic enzymes; Remazol brilliant blue R; White-rot fungi.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703463
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Degradation of lignosulfonic and tannic acids by ligninolytic soil fungi cultivated under icroaerobic conditions BABT
Silva,Isis Serrano; Menezes,Cristiano Ragagnin de; Franciscon,Elisangela; Santos,Eder da Costa dos; Durrant,Lucia Regina.
Soil fungi were evaluated regarding their ability to degrade lignin-related compounds by producing the ligninolytic enzymes. Lignosulfonic and tannic acids were used as sole carbon sources during 30 days under microaerobic and very-low-oxygen conditions. The fungi produced lignin-peroxidase, manganese-peroxidase and laccase . Expressive degradations was observed by C18 reversed-phase HPLC, indicating the biodegradation potential of these fungi, showing more advantages than obligate anaerobes to decontaminate the environment when present naturally.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lignosulfonic acid; Tannic acid; Ligninolytic enzymes; Microaerobic conditions; Soil fungi.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300026
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Fed-batch decolorization of Poly R-478 by Trametes versicolor BABT
Moreira,María Teresa; Viacava,César; Vidal,Gladys.
Physiological aspects were evaluated to determine optimal conditions for the decolorization of a synthetic dye, Poly R-478, by white-rot fungus Trametes versicolor # 52J. The decolorization experiments were carried out in semicontinuous operation during three cycles to improve the process efficiency. The best decolorization efficiencies (65% to 80%) were obtained in fungal cultures performed in nitrogen limited conditions under aerobic conditions.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Decolorization; Trametes versicolor; Poly R-478; Ligninolytic enzymes.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200003
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Ligninolytic fungus Polyporus sp. S133 mediated metabolic degradation of fluorene BJM
Lazim,Zainab Mat; Hadibarata,Tony.
ABSTRACT This study aimed to investigate the impact of nonionic surfactants on the efficacy of fluorine degradation by Polyporus sp. S133 in a liquid culture. Fluorene was observed to be degraded in its entirety by Polyporus sp. S133 subsequent to a 23-day incubation period. The fastest cell growth rate was observed in the initial 7 days in the culture that was supplemented with Tween 80. The degradation process was primarily modulated by the activity of two ligninolytic enzymes, laccase and MnP. The highest laccase activity was stimulated by the addition of Tween 80 (2443 U/L) followed by mixed surfactant (1766 U/L) and Brij 35 (1655 U/L). UV-vis spectroscopy, TLC analysis and mass spectrum analysis of samples subsequent to the degradation process in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metabolites; Ligninolytic enzymes; Polyporus sp. S133; Non-ionic surfactants; Solubilization.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300610
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Production of phenol-oxidases and peroxidases by fungi isolated from irrigated rice BJM
Silva,Célia Maria Maganhotto de Souza; Melo,Itamar Soares de; Vieira,Rosana Faria.
The aim of this work was to study the potential of fungus strains considered as prospective degraders for the herbicides quinclorac and propanil, for ligninolytic enzyme production. Eight fungal strains were grown in King's B liquid culture medium supplemented with 0.05% Remazol Brilliant Blue R (RBBR) and in liquid culture medium containing wheat bran as substrate. The enzymatic system assessment were: lignin peroxidase, manganese peroxidase and laccases. Results indicated differential patterns of ligninolytic enzyme production; the highest enzymatic activities were related to the production of lignin peroxidase. Among the strains, two (P3SA1F and P11SA2F) showed RBBR discoloration, suggesting the possibility of their application in bioremediation studies.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ligninolytic enzymes; Laccases; Remazol Brilliant Blue R.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500018
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Screening for ligninolytic enzymes from autochthonous fungi and applications for decolorization of Remazole Marine Blue BJM
Erden,Emre; Ucar,M. Cigdem; Gezer,Tekin; Pazarlioglu,Nurdan Kasikara.
This study presents new and alternative fungal strains for the production of ligninolytic enzymes which have great potential to use in industrial and biotechnological processes. Thirty autochthonous fungal strains were harvested from Bornova-Izmir in Turkiye. In the fresh fruitbody extracts laccase, manganese peroxidase and lignin peroxidase activities, which are the principal enzymes responsible for ligninocellulose degradation by Basidiomycetes, were screened. Spores of some of the basidiomycetes species such as Cortinarius sp., Trametes versicolor, Pleurotus ostreatus, Abortiporus biennis, Lyophyllum subglobisporium, Ramaria stricta, Ganoderma carnosum, Lactarius delicious ve Lepista nuda were isolated and investigated optimum cultivation conditions in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Basidiomycetes; Ligninolytic enzymes; Decolorization.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200026
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Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization Electron. J. Biotechnol.
Elgueta,Sebastián; Rubilar,Olga; Lima,Nelson; Diez,M. Cristina.
Six strains of white-rot fungi isolated from southern Chile were evaluated for their ergosterol/biomass correlation and ligninolytic potential in solid medium to formulate pellets for Reactive Orange 165 (RO165) decolourization. The fungus Anthracophyllum discolor was selected to formulate complex pellets (fungal mycelium, sawdust, and activated carbon), coated pellets (complex pellet + alginate) and simple pellets (fungal mycelium). The activity of ligninolytic enzymes (laccase, manganese peroxidase, manganese-independent peroxidase, and lignin peroxidase) was evaluated in both the complex and coated pellets in modified Kirk medium, and the morphology of the pellets was studied using scanning electron microscopy (SEM). Complex pellets of A. discolor...
Tipo: Journal article Palavras-chave: Complex pellets; Ligninolytic enzymes; RO165 decolourization; White-rot fungi.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600008
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Solid-state bioconversion of passion fruit waste by whiterot fungi for production of oxidative and hydrolytic enzymes. Repositório Alice
ZILLY, A.; BAZANELLA, G. C. S.; HELM, C. V.; ARAÚJO, C. A. V.; SOUZA, C. G. M.; BRACHT, A.; PERALTA, R. M..
2011
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Enzima lignocelulósica; Fermentação sólida; Laccase; Ligninolytic enzymes; White-rot fungi; Solid-state fermentation.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/912761
Registros recuperados: 11
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